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Abstract Source rocks are found in the Eocene Liushagang Formation (El 1: bottom , El 2 and El 3:top members), in the Weixi'nan sag, Beibuwan Basin. The source rocks are mainly mudrocks, except El 2 , which consists of organic-rich shales at the bottom and mudrocks in the middle-upper part. Total organic carbon (TOC) and Rock-Eval analyses of 146 samples indicate that El 2 rocks have the best hydrocarbon generating potential, especially for organic-rich shales that contain up to 10% TOC with HI > 800 mg/g. The vitrinite reflectance analysis suggests that El source rocks are marginally mature to mature. Geochemical analyses were conducted on 18 El rock extracts and 18 oil samples from the WZ12 field. El 2 and El 3 source rock have similar organic facies and are distinct from El 1. El 2 and El 3 have higher C 27 βα (20R + 20S) diasteranes/C 27 αββ (20R + 20S) steranes (C 27 DiaS/C 27 S), Gammacerane/Hopane (G/H) and (C 28 + C 29) tricyclic terpane/((C 28 + C 29) tricyclic terpane + Ts) (ETR), and low C 23 tricyclic terpane/hopane (C 23 /H) and C 24 tetracyclic terpanes/C 26 tricyclic terpane (C 24 /C 26). These biomarker distributions indicate that El 2 and El 3 rocks were deposited under more alkaline-saline environment and had less import of terrestrial organic matter relative to El 1. El 2 can be distinguished from El 3 as having higher 4-methylsteranes/C 29 ααα steranes (4MSI) and G/H. WZ12 oils all exhibit similar compositions and display biomarker characteristics that correlate with the El 2 and El 3 rock extracts. 1D thermal and burial history model shows that El 2 and El 3 began to generate a great deal of hydrocarbon at ∼11.5 Ma and 23 Ma, respectively. At this stage, traps formed and faults actively extended downwards into the El 2 and likely into El 3. Oils expelled from El 2 and El 3 migrated upward into shallow intervals along faults then laterally along the structural slopes and accumulated in areas with good fault sealing primarily in the Miocene age Nx and Nj strata. Meanwhile, under the effect of overpressure in the middle of organic-rich shales, oils from El 2 may have migrated downward into reservoirs in the upper part of El 3. Lithologic traps in El 1 reservoir may be charged with relatively immature oil derived from El 1. Highlights • Source rocks in El 2 , especially organic-rich shales in the bottom of El 2 are the most important source rocks. • Sedimentary environment of different intervals of El have been distinguished. • WZ12 oils generated from oil El 2 and El 3 source rocks based on biomarkers parameters of saturated hydrocarbon. • Faults and overpressure may be responsible for the distribution of WZ12 oils. [ABSTRACT FROM AUTHOR]

Abstract As lanthanum oxide (La 2 O 3) has a large bandgap, it exhibits excellent optical properties. In this work, La 2 O 3 thin films are prepared by the sol-gel method from a lanthanum chloride (LaCl 3) precursor, on quartz and sapphire (0001) substrates. The effect of the annealing temperature (400–800 °C) on the optical properties of the La 2 O 3 films is investigated. The structure and optical properties are analyzed by X-ray diffraction, atomic force microscopy, scanning electronic microscopy, X-ray photoemission spectroscopy, Raman spectroscopy, ultraviolet (UV)–visible spectroscopy, Fourier transform infrared spectroscopy, and infrared variable angle spectroscopic ellipsometry. Results indicate that with an annealing temperature of ~600 °C, the roughness and optical properties of La 2 O 3 films can be significantly improved. Highlights • La 2 O 3 thin films are prepared by sol-gel method on quartz and sapphire substrates. • Effect of annealing temperature on their optical properties is investigated. • Results indicate that the best annealing temperature is around 600 °C. • The roughness and optical properties of films are significantly improved. [ABSTRACT FROM AUTHOR]

The shale from the Cretaceous Taizhou Formation is an important source rock in the Gaoyou Sag, Northern Jiangsu Basin. Fifty-three shale core samples analyzed for total organic carbon (TOC) and Rock-Eval pyrolysis analyses were used to evaluate the geochemistry of this shale. Twenty-one samples of them were analyzed using solvent extraction, extract fractionation, Gas Chromatography-Mass Spectrometer (GC-MS) of the saturated fraction, and organic petrology analyses. Based on the test results, the shale of the Taizhou Formation contains mainly Type II-III organic matter and is chiefly oil-prone and oil-gas-prone, as a fair to good oil-source rock with a small quantity of gas-source rock. Vitrinite reflectance, Rock-Eval pyrolysis, and biomarker suggest that in this sag, the shale of the Taizhou Formation is mainly in the early mature stage and within the oil window, so the shale generates chiefly liquid oil. The Gaoyou Sag is an oil-generating sag. The saturated fraction and biomarker composition of the shale provide evidence that the Taizhou Formation shale was formed mainly in a reductive sedimentary environment with anoxic fresh to low salinity water. The organic matter came chiefly from plankton and partly from land plants and bacterial organisms. [ABSTRACT FROM AUTHOR]

The shales in the Baijiantan Formation are considered to be an important potential source rock in the Junggar Basin. To evaluate this basin’s source-rock characteristics, 61 core samples from 14 boreholes were analyzed using total organic carbon and total sulfur analyses and Rock-Eval pyrolysis. Thirty-two core samples were analyzed using solvent extraction, column fractionation, biomarker analysis and the organic petrology of kerogen. The results suggest that the shales in the Baijiantan Formation have fair to excellent hydrocarbon generation potential, and the organic matter types include both Type II and Type III. The shales in the Baijiantan Formation are mainly oil-generating rocks, and better source rocks are mainly distributed in the middle–lower section of the Baijiantan Formation. The total organic carbon and total sulfur analyses, Rock-Eval pyrolysis, and biomarkers indicate that the shales in the Baijiantan Formation formed in a low-salinity to freshwater lacustrine sedimentary environment under reducing conditions. Biomarkers indicate that the organic matter input of the shales exhibits varying contributions from plankton, algae and terrestrial organisms. The biomarker molecular composition of the source rock is related to the organic matter source, salinity, redox conditions and maturation. [ABSTRACT FROM AUTHOR]

Hybridization and polyploidization can be major mechanisms for plant evolution and speciation. Thus, the process of polyploidization and evolutionary history of polyploids is of widespread interest. The chloroplast DNA regions trnH - psbA was used to analyze to phylogenetic relationships and maternal donor of Elymus species and their closely related species. The Neighbor-Joining phylogenetic reconstructions partitioned the Elymus species into two groups. All the Elymus species were related to species of Pseudoroegneria . These results indicated that (1) Pseudoroegneria (St genome) was the maternal donor of the polyploidy Elymus ; (2) the St genome of Elymus had several origins and diverse species of Pseudoroegneria might have taken part in the formation of polyploid species of Elymus ; (3) high degree genome differentiation exists among the Pseudoroegneria species. [ABSTRACT FROM AUTHOR]

The Zingiberales is an ancient monocot order in the angiosperms. It has undergone radiation since the Cretaceous period. However, the relationships among primary lineages (families) are difficult to resolve. Additional support for phylogenetic relationships within this order have been obtained using plastid genome data from 76 coding genes and 4 rDNA loci from 17 species represented eight families. We reconstructed ancestral geographic areas using the Bayesian Binary Markov Chain Monte Carlo (BMM) method. These analyses indicated several major radiations of Zingiberales occurring in Africa, Neotropical America and Australia. [ABSTRACT FROM AUTHOR]

Functional screening of a metagenomic library of termite hindgut identified an overlapping gene cluster encoding the phosphotransferase system (PTS) components, which consisted of a glucoside specific PTS enzyme II gene ( glu1923 ) and a glycoside hydrolase gene ( glu1392 ). Hydrolytic experiments revealed that the combined effect of Glu1923 and Glu1392 was responsible for the utilization of glucosidic substrates in recombinant Escherichia coli ( E. coli ) strains. Yeast two hybrid analysis suggested that there was an interaction between Glu1923 and Glu1392, and the domain EIIA of Glu1923 played an important role for the interaction. In addition, the hydrolase Glu1392 displayed hydrolysis ability toward cellobiose and maltose, and had a very high tolerance to glucose with a K i value of 2.25 M. These properties make Glu1392 an interesting candidate in biotechnology applications after further study. [ABSTRACT FROM AUTHOR]

Shale oil exploration and development have been conducted in the Permian Lucaogou Formation in the Jimusaer Sag, Junggar Basin. The upper (P 2 l 2) and lower (P 2 l 1) members of the Lucaogou Formation developed with sand layers, which are the shale oil development sections in the Lucaogou Formation. In order to clarify the enrichment law of shale oil for these members, the geological conditions of the P 2 l 2 and P 2 l 1 members were analyzed. The focus of this study were oil-bearing sandstone and mudstone samples from the Lucaogou Formation. These samples were studied by casting thin section observation, total organic carbon, pyrolysis, and gas chromatography–mass spectrometry. The results showed that the upper and lower members of the Lucaogou Formation are mainly characterized as interbedded source rock and reservoir. An oil-source correlation showed that the crude oil in the upper member mainly originated from its adjacent source rock from a deeper position through secondary migration. However, no evidence of secondary migration was observed in the crude oil in the lower member which mainly originated from the adjacent source rock. For the sandbodies in the upper member, when burial is shallower than peak oil generation, the physical properties of the middle part of the sandbody are better than the top and bottom. When burial is deeper than peak oil generation, relatively good physical properties developed in the sandstone in the vicinity the source rock. The lower member has less reservoir thickness, poorer lateral continuity and horizontal permeability than the upper member. The high-quality reservoirs are represented in a single sandbody in the lower member that is mainly developed in the middle part. After the crude oil was discharged from the source rock enters the sandbody, and long-distance lateral migration was restricted. Thus, crude oil in the lower member is enriched and accumulated in situ. • The characteristics of the source rocks and reservoir of the upper and lower members of the Lucaogou Formation are described. • The oil-source correlation in the upper and lower reservoirs of the Lucaogou Formation have been discussed. • The oil accumulation model of the upper and lower member of the Lucaogou Formation is established. [ABSTRACT FROM AUTHOR]

Abstract: A new triterpenoid saponin, named semenoside A (1), was isolated from Semen Celosia cristatae. Its structure was elucidated on the basis of 1D, 2D NMR, HR-FAB-MS and ESI-MS techniques, and physicochemical properties. The hepatoprotective activity of semenoside A with an oral dose of 1.0, 2.0 and 4.0mg/kg, respectively, were investigated by carbon tetrachloride (CCl4)-induced hepatotoxicity in mice. The results indicated that it had significant hepatoprotective effects (p <0.01). [Copyright &y& Elsevier]

Abstract: Constituents of the fruits of Peatasites tatewakianus were investigated. A new compound, namely bakkenolide-VI (1), was isolated. The structures was elucidated on the basis of 1D, 2D NMR, TOF-MS and ESI-MS techniques, and physicochemical properties. The neuroprotective activity of the new compound was assayed with primary cultured neurons exposed to oxygen–glucose deprivation and oxidative insults. [Copyright &y& Elsevier]

• Transcriptome and proteome profiles revealed global view of ramie regulation mechanism responding to hydroponic culturing compared to soiled culturing. • 24 BnALDH genes grouped in 8 ALDH superfamily, mainly functioned in roots, is responsible for the quick and stable adaptation of ramie to hypoxic aquatic environment through acetaldehyde detoxification and osmotic protection. • BnALDH18 is a promising and untapped gene for improving oxygen deficiency tolerance in ramie under hydroponic culturing, which was proved by its heterologously overexpression in Escherichia coli cells. It is an increasing interest in developing a hydroponic culture system for ramie propagation, which provide a promising method for blocking-up the soilborne diseases. However, there is an urgent need for better understanding of ramie adaptation mechanism during hydroponic culturing under low-oxygen condition. In this study, a genome-wide study plus transcriptomic and proteomic analysis was conducted to profile the molecular responses related to ramie oxidative adaption. The results showed that aldehyde dehydrogenases (ALDHs) played important roles in molecular oxygen deficiency and anaerobic metabolism in ramie. Totally, 24 ALDH genes in ramie genome were identified and grouped into 8 superfamily. All of these 24 BnALDH genes, which are unevenly distributed on the 12 chromosomes, exhibited differential expression patterns under hydroponic and soiled culturing. The expression level of almost all the BnALDHs in leaves are lower than in the roots. The BnALDHs classified into family 2 and family 10 were up-regulated in the hydroponic cultured ramie roots. Especially, BnALDH18 belonging to family 10 showed the strongest response to low-oxygen stress. In addition, heterologous overexpression analysis of BnALDH18 in Escherichia coli showed its function for elevating tolerance to oxidative stress. The genome-wide identification and analysis of evolution and expression manner demonstrated that BnALDH s are promising and untapped genetic resources for improving oxygen deficiency tolerance of ramie under hydroponic culturing. [ABSTRACT FROM AUTHOR]

According to the geochemistry, organic petrography, stable carbon isotopic compositions and biomarker analyses of the Late Miocene Zeit Formation shale samples in the Tokar area, Red Sea Basin, the paleoenvironment and hydrocarbon-generating potential were analyzed. The Zeit Formation shales from the Wells S. Suakin-1 and Tokar-1 contain mostly Type III kerogens with minor Type II organic matter (OM), indicating more favorable gas-generating potential. OM of the shale samples from the Late Miocene Zeit Formation are predominantly of terrigenous higher plants. Kerogens from the Well Tokar-1 show abundant brown liptinites, indicating the mixed origin of planktonic/bacterial and terrigenous OM. The Late Miocene Zeit Formation was deposited in dysoxic/suboxic, reducing and salt water environments. According to the results of Tmax, Ro and biomarker maturity parameters, the most shale samples from the Zeit Formation are in early mature to mature stages. The shales from the Late Miocene Zeit Formation are generally fair to good source rock. The shales from wells Suakin-2, Talla-2 and Tokar-1 have high hydrocarbon-generating potential. • OMs from the Zeit Fm. Are predominantly of terrigenous higher plants. • Shales were deposited in dysoxic/suboxic, reducing and salt water environments. • Shales from the Zeit Formation are in early mature to mature stages. • Shales from the Zeit Formation are generally fair to good source rock. [ABSTRACT FROM AUTHOR]

The Lower Cretaceous Xiagou Formation in Qingxi Sag consists of more than 1000 m of lacustrine sediments, including dolomitic mudstone/argillaceous dolomite and sandstone/mudstone, which is considered to be the most important source rock in the Jiuquan Basin. Power spectra, evolutionary fast Fourier transform (FFT), and correlation coefficient analyses of the gamma-ray (GR) series from four wells, including Liu 8 well, Long 14 well, Q2-36 well and Liu 9 well, through the Xiagou Formation reveal strong astronomical signals of long eccentricity (405 kyr), short eccentricity (100 kyr), obliquity (37 kyr) and precession (22 kyr). These provide an excellent opportunity to establish a floating astronomical time scales (ATS) then to determine variations in the sediment accumulation rate (SAR). Based on the floating ATS of the Xiagou Formation, the Xiagou Formation covers a 10.3 myr period depositional duration, and depositional duration of three members in the Xiagou Formation are 3.5 ± 0.1 myr, 3.1 ± 0.1 myr and 3.65 ± 0.15 myr, respectively. The high-resolution SARs of the Xiagou Formation vary from 8.5 cm/kyr to 19.8 cm/kyr. The different lithologies in the Xiagou Formation exhibit the same variation tendency in SARs, but the mechanisms of astronomical forcing that affect the SARs are different. In addition, the correlation between total organic carbon (TOC) and SARs can be used to investigate the impact of the SAR on organic matter abundance and conclude that relatively high SAR can protect organic matter and prevent oxidation, but rapid SAR can also dilute organic matter abundance of source rock. [ABSTRACT FROM AUTHOR]

In order to study the organic matter source in stylolite and its effect on hydrocarbon migration, carbonate rock core samples containing stylolites in the eastern Sichuan Basin were collected. The stylolite and matrix in the cores were analyzed by total organic carbon (TOC), Rock-Eval pyrolysis and gas chromatography-mass spectrometer (GC–MS) for the saturated fractions. The organic matter is more enriched in the stylolite than the matrix, and the stylolite have higher hydrocarbon expulsion efficiency than the matrix, which can be used as a supplement to the later generation of the source rock. The hydrocarbons formed by the matrix aggregated near the stylolites and cause the Hydrocarbon Index of the matrix which adjacent to the stylolites to become higher, this indicates that the stylolite is an important channel for hydrocarbon discharge of carbonate rocks. The ratios of close molecular biomarker in stylolite indicate that the deposition environment, biological origin and maturity of organic matter in the stylolites are consistent with the matrix, therefore, the organic matter in the stylolite should be inherited from the matrix, but the light to heavy molecular biomarker ratios of the stylolite indicate that component fractionation occurred when the hydrocarbons generated in the matrix migrated to the stylolite. Especially, a series of light to heavy molecule ratios of C 19 to C 23 tricyclic terpanes and C 14 to C 19 normal alkane of stylolite are lower than that of matrix, this indicates that the hydrocarbon fractionation in the course of hydrocarbon migration along the stylolite is more obvious than that in the matrix. • The organic-rich stylolite in low-abundance carbonate source rocks is an important supplement to the parent material for hydrocarbon generation. • The stylolite is important pathway for fluid migration in carbonate rocks. • The hydrocarbons in the matrix underwent a fractionation effect as they migrated from the matrix to the stylolite. • The hydrocarbon fractionation in the course of hydrocarbon migration along the stylolite is more obvious than that in the matrix. [ABSTRACT FROM AUTHOR]

Shale oil generated by the lower member of the Lucaogou Formation, which was deposited under saline lacustrine conditions within the Jimusaer Sag of the Junggar Basin (NW China), is generally characterized by high density and viscosity. However, a strong spatial heterogeneity of physical properties is observed as well. In this paper, these spatial variations and their controlling mechanisms are evaluated from the perspective of the primary depositional environment, source rock thermal evolution and fractionation effects of primary migration. Based on a detailed organic petrological and geochemical characterization of the target interval within the key well JHBE, hydrous pyrolysis experiments conducted on representative source rock samples from well JHBE, as well as established methods of oil-source correlation subjected to oil samples from several regionally distributed producing shale oil wells, a predictive model of density and viscosity changes of crude oil was established. According to the findings of this study, the thermal evolution and sedimentary environment of source rocks are the main factors affecting the quality of crude oil, and the fractionation effect of primary migration has little effect. Crude oil density and viscosity at first increase up to peak oil maturity, followed by a decrease with further increasing thermal evolution of the source rock. Furthermore, if the source rocks experienced similar thermal histories, it was discovered that relatively low density and corresponding viscosity values are characteristic for crudes from source rocks which were formed in a relatively high salinity environment. In contrast, hydrocarbon generation of source rocks formed under relatively low salinity environments is slightly retarded in direct comparison, and the density and viscosity of the generated crude oil are both relatively high. This highlights that a detailed organic petrographical and geochemical evaluation of the target formation is crucial for the assessment of shale oil potential and production sweet spots in alginite-dominated lacustrine sedimentary environments. • Oil-source correlation of the lower member in the Lucaogou Formation are conducted. • Crude oil density and viscosity control by thermal evolution are discussed from a new sight. • The higher salinity is, the lower density of crude oil is. [ABSTRACT FROM AUTHOR]

[Display omitted] • 30 β -carboline-(phenylsulfonyl)furoxan hybrids were designed and synthesized. • Target molecules showed potent anti-breast cancer proliferative activity. • 13h displayed potent antiproliferative activity against MDA-MB-231 cells. • 13h suppressed the migration and invasion of MDA-MB-231 cells. • ROS generation and activating DNA damage involved in 13h -induced apoptosis. The cytotoxicity properties of the β -carboline alkaloids have been broadly investigated. However, the potential application of β -carbolines was hindered due to the moderate activity in cancer. In the present study, thirty β -carboline-(phenylsulfonyl)furoxan hybrids (11a–j , 12a–j and 13a–j) were designed and synthesized through esterification and amidation reaction strategy, and their inhibitory activities against the human breast cancer cell lines MCF-7 and MDA-MB-231 were evaluated by CCK-8 assay. Biological evaluation presented that the most promising amide derivative 13h , substituted with p -methoxyphenyl group at position 1, generated high concentration of NO and evidently depressed the MCF-7 (IC 50 = 0.89 μM) and MDA-MB-231 (IC 50 = 0.62 μM) cells proliferation. Particularly, the wound healing and transwell assays demonstrated that 13h significantly inhibited the migration and invasion of MDA-MB-231cells. Furthermore, the preliminary mechanisms studies indicated that 13h induced G2/M phase arrest and apoptosis possibly causing by ROS accumulation and ROS-mediated DNA damage. Based on these considerations, 13h may be a promising antimetastatic agent for breast cancer, which is noteworthy for further exploration. [ABSTRACT FROM AUTHOR]

This research reveals the depositional environment can affect the organic matter enrichment. The major elements and trace elements are important indexes to reconstruct the depositional environment of Ordovician subsalt marine formations in the central-east Ordos Basin, combining organic parameters with inorganic parameters. Based on the microfacies, the study area is divided into gypsum-bearing dolomite flat, dolomite flat, gypsum-salt depression and limy dolomite flat. The higher Ti/Al ratios and the characteristics of trace elements show that there were detrital inputs in the sedimentation process. When the amounts of detrital inputs are large enough, they can obviously adsorb the elements and dilute the organic matter. Therefore, gypsum-bearing dolomite flat and dolomite flat have lower organic matter accumulation with higher detrital input. Lower P/Ti and Ba excess indicate the deficiency of paleoproductivity. Sr/Ba is higher than 0.6, indicating marine-continental transitional and marine environment. Even the paleosalinity is higher, previous researches have demonstrated the descending sea levels and strong hydrodynamic conditions are unfavorable for organic matter preservation. Lower paleoclimate index "C" and higher Sr/Cu illustrate arid and hot conditions. The value of V/(V + Ni) suggests that the limy dolomite flat is under the oxidizing conditions, and the three other environments are reductive to a certain extent. The Mo-U covariation shows that the water body is under strongly restricted conditions. These conditions are not conducive to biological growth and the accumulation of organic matter, causing low ancient productivity. The partial correlation analysis shows: the organic matter enrichment is affected by multiple factors; paleoproductivity, paleosalinity and paleoclimate have greater influence on the results of organic matter enrichment, and detrital input and oxidation conditions can decrease the paleoproductivity, indicating multiple factors are interrelated and interact with each other. • Major and trace elements are used to reconstructs the depositional environment of four types microfacies. • Paleoproductivity, paleosalinity and paleoclimate have greater influence on the organic matter enrichment. • Depositional environment has direction relationship with low paleoproductivity, causing low organic matter enrichment. [ABSTRACT FROM AUTHOR]

Herein, highly transparent and conductive In 2 O 3 films were deposited by reactive high power impulse magnetron sputtering from a metal target without thermal assistance. Film deposition was performed at room temperature using oxygen flow rates selected based on target voltage hysteresis, and the obtained films were compared in terms of structure and electrical/optical properties. Notably, the use of high plasma density, peak power, and ionization efficiency resulted in the formation of highly crystalline films featuring a small grain size and a columnar structure with a preferred orientation. The minimal carrier concentration of 1.6 × 1021 cm−3 was observed for films deposited in metallic mode; the carrier mobility of these films increased from 23 to 51 cm2 V−1 s−1 as the oxygen flow rate increased from 7 to 10 sccm. Moreover, the oxygen flow rate significantly affected the conductivity of In 2 O 3 films, which ranged from semiconductor-like to insulator. The visible-light transmittance of In 2 O 3 films exceeded 85% when oxygen flow rate larger than 7sccm. The absorption edge of as-prepared films was located close to 350 nm and shifted to lower wavelengths with increasing oxygen flow rate, which was explained by the Burstein–Moss effect. The infrared transmittance of In 2 O 3 films was strongly dependent on the deposition mode. In agreement with Drude's theory, films deposited in transition mode at room temperature were highly transparent and conductive, which was ascribed to the use of controlled In:O ratios, whereas films deposited in other modes were shown to exhibit either excellent conductivity (metallic mode) or good optical transmittance (poisoning mode). Image 1 [ABSTRACT FROM AUTHOR]

Ramie is an ideal crop for remediation of cadmium (Cd) contaminated soil. However, there is a lack of rapid and effective evaluation system for Cd tolerance of ramie germplasms, and also a lack of systematic and in-depth research under Cd contaminated field conditions. This study innovatively developed a rapid screening system of "hydroponics-pot planting", and 196 core germplasms were used to quickly and effectively identify their Cd tolerance and Cd enrichment capacity. Then, two excellent varieties were selected to carry out a 4 years of field experiment under Cd contaminated field to study the remediation model, evaluation of reuse after repair and the mechanism of microbial regulation. The results showed that ramie adopted the cycle mode of "Absorption-activating soil Cd-Migration-Absorption" to remediate on Cd contaminated field, and the application of ramie for remediation had good ecological and economic benefits. Ten dominant genera such as Pseudonocardiales , as well as the key functional genes (mdtC, mdtB, mdtB/yegN, actR, rpoS, and ABA transporter gene) in rhizosphere soil, were identified to participate in activating Cd in rhizosphere soil and promoting ramie to enrich Cd. This study provides a technical route and practical production experience for the research field of phytoremediation of heavy metal pollution. [Display omitted] • A rapid screening system was innovatively developed to identify Cd tolerant ramie germplasms. • A cycle remediation on ramie was revealed base on a field long-term remediation experiment. • Microbial regulation mechanism was revealed on field experiment. • Key genes in rhizosphere soil were identified to involve in regulating ramie to absorb Cd. [ABSTRACT FROM AUTHOR]