1. miR-4757-3p Inhibited the Migration and Invasion of Lung Cancer Cell via Targeting Wnt Signaling Pathway.
- Author
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Zhao, Pei, Zhao, Qian, Chen, Chen, Lu, Song, and Jin, Li
- Subjects
IN vitro studies ,CANCER invasiveness ,LUNG tumors ,MICRORNA ,WNT proteins ,CELLULAR signal transduction ,BIOINFORMATICS ,GENE expression ,CELL motility ,CELL migration inhibition ,GENE expression profiling ,DATA analysis software ,POLYMERASE chain reaction ,CELL lines - Abstract
Lung cancer accounts for the vast majority of cancer-related deaths worldwide, and aberrant miRNA expression is commonly observed as the disease progresses. The current study aimed to determine the role of miR-4757-3p in the development of lung cancer. The real-time PCR test was performed to determine the expression of miR-4757-3p in lung cancer cell lines. miR-4757-3p was downregulated in A549 cells. CCK8 and transwell assays demonstrated that overexpression of miR-4757-3p significantly reduced A549 cell invasion and migration. Bioinformatic analysis by the TargetScan database predicted the possible targets of miR-4757-3p. A luciferase activity test was used to determine the direct relationship between miR-4757-3p, Wnt5a, and Wnt8b. The overexpression of miR-4757-3p drastically inhibited the expression of Wnt5a and Wnt8b. Furthermore, we discovered that silencing Wnt5a and Wnt8b significantly lowered β-catenin expression and hampered invasion and migration. Finally, miR-4757-3p inhibited lung cancer cell migration and invasion by inhibiting the activation of the Wnt signaling pathway. Our study provided evidence that miR-4757-3p could be developed as an indicator or an anticancer target in the clinical application. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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