1. miR-4757-3p Inhibited the Migration and Invasion of Lung Cancer Cell via Targeting Wnt Signaling Pathway.
- Author
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Zhao, Pei, Zhao, Qian, Chen, Chen, Lu, Song, and Jin, Li
- Subjects
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IN vitro studies , *CANCER invasiveness , *LUNG tumors , *MICRORNA , *WNT proteins , *CELLULAR signal transduction , *BIOINFORMATICS , *GENE expression , *CELL motility , *CELL migration inhibition , *GENE expression profiling , *DATA analysis software , *POLYMERASE chain reaction , *CELL lines - Abstract
Lung cancer accounts for the vast majority of cancer-related deaths worldwide, and aberrant miRNA expression is commonly observed as the disease progresses. The current study aimed to determine the role of miR-4757-3p in the development of lung cancer. The real-time PCR test was performed to determine the expression of miR-4757-3p in lung cancer cell lines. miR-4757-3p was downregulated in A549 cells. CCK8 and transwell assays demonstrated that overexpression of miR-4757-3p significantly reduced A549 cell invasion and migration. Bioinformatic analysis by the TargetScan database predicted the possible targets of miR-4757-3p. A luciferase activity test was used to determine the direct relationship between miR-4757-3p, Wnt5a, and Wnt8b. The overexpression of miR-4757-3p drastically inhibited the expression of Wnt5a and Wnt8b. Furthermore, we discovered that silencing Wnt5a and Wnt8b significantly lowered β-catenin expression and hampered invasion and migration. Finally, miR-4757-3p inhibited lung cancer cell migration and invasion by inhibiting the activation of the Wnt signaling pathway. Our study provided evidence that miR-4757-3p could be developed as an indicator or an anticancer target in the clinical application. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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