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Background: Circular RNAs (circRNAs) have been implicated in the pathogenesis of atherosclerosis (AS) and the migration and proliferation of vascular smooth muscle cells (VSMCs) under oxidized low-density lipoprotein (ox-LDL). Here, we defined the exact action of human circ_0007478 in VSMC migration and proliferation induced by ox-LDL. Methods: Human VSMCs (HVSMCs) were exposed to ox-LDL. Circ_0007478, microRNA (miR)-638, and rho-associated protein kinase 2 (ROCK2) levels were gauged by quantitative real-time PCR (qRT-PCR) and western blot. Cell viability and proliferation were assessed by MTT and EdU assays, respectively. Transwell assays were used to detect cell migration and invasion. Dual-luciferase reporter and RNA immunoprecipitation (RIP) assays were used to evaluate the direct relationship between miR-638 and circ_0007478 or ROCK2. Results: Our data indicated that circ_0007478 expression was augmented in AS serum samples and ox-LDL-treated HVSMCs. Depletion of circ_0007478 attenuated HVSMC proliferation, migration, and invasion induced by ox-LDL. Mechanistically, circ_0007478 targeted miR-638 by directly pairing to miR-638. Reduction of miR-638 reversed the effects of circ_0007478 depletion on ox-LDL-evoked proliferation, migration, and invasion in HVSMCs. ROCK2 was a direct miR-638 target and miR-638-mediated inhibition of ROCK2 relieved ox-LDL-evoked HVSMC proliferation, migration, and invasion. Furthermore, circ_0007478 was identified as a competing endogenous RNA (ceRNA) for miR-638 to modulate ROCK2 expression. Conclusion: Our present study establishes an undescribed ceRNA regulatory network, in which circ_0007478 targets miR-638 to upregulate ROCK2, thereby contributing to ox-LDL-induced proliferation and migration in HVSMCs. [ABSTRACT FROM AUTHOR]

In the present study, we aim to examine the mediating roles of the two types of perceived parental influence in the relation between the dual filial piety model and adult children's long-term mate preferences. A survey was administered to 499 Chinese adult children on their filial piety beliefs, perceptions about parental influence, and long-term mate preferences. Structural equation modeling revealed the following findings. (a) For both genders, reciprocal filial piety was positively correlated with good father/mother traits, and authoritarian filial piety was positively correlated with good gene traits. (b) For both genders, sensitivity from the perceived parental influence mediated the link between authoritarian filial piety and good provider traits. (c) For females, sensitivity mediated the link between authoritarian filial piety and good gene traits. (d) For males, parental monitoring from perceived parental influence mediated the link between authoritarian filial piety and good gene and good mother traits. In addition, authoritarian filial piety was positively correlated with good provider and good mother traits. In summary, filial piety can link both directly and indirectly to mate preference through parental sensitivity for males and females and through parental monitoring mainly for males. [ABSTRACT FROM AUTHOR]