1. Detection and molecular characterization of a novel piscine-myocarditis-like virus from baitfish in the USA.
- Author
-
Mor, Sunil and Phelps, Nicholas
- Subjects
- *
MYOCARDITIS , *CARDIOMYOPATHIES , *MYOSITIS , *BAITFISH - Abstract
During a survey of apparently healthy baitfish from the state of Minnesota, a novel piscine-myocarditis-like virus (PMCLV) was detected in golden shiners ( Notemigonus crysoleucas). The nearly complete genome sequence is 5819 nt long, including a partial 5' untranslated region (UTR) of 100 nt. The sequence is divided into three ORFs: the complete ORF1 and ORF2, encoding proteins of 818 and 831 amino acids, respectively, and a partial ORF3 encoding 248 amino acids of the corresponding protein. This novel virus sequence was most closely related to piscine myocarditis virus (PMCV), with a 49.0 % and 58.2 % amino acid identity in the ORF1 (YP005481249)- and ORF2 (YP004581250)-encoded proteins, respectively. Six of 56 retail outlets (e.g., bait shops) were positive during the 2014-2015 survey, indicating a 10.7 % prevalence of the novel virus in this population of golden shiners. Currently, there is no disease that is known to be associated with this virus. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF