1. Expression of TCR-Vβ peptides by murine bone marrow cells does not identify T-cell progenitors.
- Author
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Abbey, Janice L., Karsunky, Holger, Serwold, Thomas, Papathanasiou, Peter, Weissman, Irving L., and O'Neill, Helen C.
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PEPTIDES ,BONE marrow cells ,HEMATOPOIETIC system ,PROTEINS ,T cells - Abstract
Germline transcription has been described for both immunoglobulin and T-cell receptor ( TCR) genes, raising questions of their functional significance during haematopoiesis. Previously, an immature murine T-cell line was shown to bind antibody to TCR-Vβ8.2 in absence of anti-Cβ antibody binding, and an equivalent cell subset was also identified in the mesenteric lymph node. Here, we investigate whether germline transcription and cell surface Vβ8.2 expression could therefore represent a potential marker of T-cell progenitors. Cells with the TCR phenotype of Vβ8.2
+ Cβ− are found in several lymphoid sites, and among the lineage-negative (Lin− ) fraction of hematopoietic progenitors in bone marrow ( BM). Cell surface marker analysis of these cells identified subsets reflecting common lymphoid progenitors, common myeloid progenitors and multipotential progenitors. To assess whether the Lin− Vβ8.2+ Cβ− BM subset contains hematopoietic progenitors, cells were sorted and adoptively transferred into sub-lethally irradiated recipients. No T-cell or myeloid progeny were detected following introduction of cells via the intrathymic or intravenous routes. However, B-cell development was detected in spleen. This pattern of restricted in vivo reconstitution disputes Lin− Vβ8.2+ Cβ− BM cells as committed T-cell progenitors, but raises the possibility of progenitors with potential for B-cell development. [ABSTRACT FROM AUTHOR]- Published
- 2015
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