23 results on '"Song, Haoming"'
Search Results
2. MHC-1B carried exosomes derived from tubular epithelial cell induced by the EGFR mimotope inhibit macrophage activation in renal fibrosis
- Author
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Guo, Jin, Liu, Xuanqi, Song, Haoming, Gu, Yong, Niu, Jianying, and Yang, Lin
- Published
- 2023
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3. Immune function of peripheral T cells in patients with venous thromboembolism or coronary artery atherosclerosis
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Zhou, Lin, Song, Haoming, Xu, Wenjun, Xu, Jiahong, Jiang, Jinfa, Gong, Zhu, Liu, Yang, Yan, Wenwen, and Wang, Lemin
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- 2014
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4. Simulation and Key Physical Drivers of Primary Productivity in a Temperate Lake during the Ice-Covered Period: Based on the VGPM Model.
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Zhang, Jie, Xie, Fei, Song, Haoming, Meng, Jingya, and Zhang, Yiwen
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BODIES of water ,LAKES ,GLOBAL warming ,WATER temperature ,ECOSYSTEMS ,FOREST productivity - Abstract
The primary productivity of seasonal ice-covered water bodies is an important variable for understanding how temperate lake ecosystems are changing due to global warming. But there have been few studies on the complete change process of primary productivity during the ice-covered period, and the connection between ice physical and associated biological production has not been fully understood. In this study, a Vertically Generalized Production Model (VGPM) suitable for the ice-covered period was used to calculate the primary productivity of a temperate lake, and the key physical controlling factor was analyzed in the process of primary productivity change in the ice-covered period. The results showed that there was a high level of primary productivity, (189.1 ± 112.6) mg C·m
−2 ·d−1 , under the ice in the study site, Hanzhang Lake. The phytoplankton production under the ice was not as severely restricted by light as commonly thought. The water temperature played a more crucial role in the changes of primary productivity than the light beneath the ice. The study highlighted the variability in primary productivity covering the whole ice-covered age, and provided a better understanding of how the aquatic environment of lakes in seasonal ice-covered areas was affected by warmer temperatures. [ABSTRACT FROM AUTHOR]- Published
- 2023
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5. LncRNA UCA1, miR‐26a, and miR‐195 in coronary heart disease patients: Correlation with stenosis degree, cholesterol levels, inflammatory cytokines, and cell adhesion molecules.
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Li, Jie, Chen, Zhisong, Wang, Xiaoyan, and Song, Haoming
- Published
- 2022
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6. Acute Pulmonary Embolism and Dysfunction of CD3+ CD8+ T Cell Immunity
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Wang, Lemin, Song, Haoming, Gong, Zhu, Duan, Qianglin, and Liang, Aibin
- Published
- 2011
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7. Motor Learning Improves the Stability of Large-Scale Brain Connectivity Pattern.
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Yu, Mengxia, Song, Haoming, Huang, Jialin, Song, Yiying, and Liu, Jia
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MOTOR learning ,FUNCTIONAL magnetic resonance imaging ,FUNCTIONAL connectivity ,MOTOR ability ,MOTOR cortex - Abstract
Repeated practice is fundamental to the acquisition of skills, which is typically accompanied by increasing reliability of neural representations that manifested as more stable activation patterns for the trained stimuli. However, large-scale neural pattern induced by learning has been rarely studied. Here, we investigated whether global connectivity patterns became more reliable as a result of motor learning using a novel analysis of the multivariate pattern of functional connectivity (MVPC). Human participants were trained with a finger-tapping motor task for five consecutive days and went through Functional magnetic resonance imaging (fMRI) scanning before and after training. We found that motor learning increased the whole-brain MVPC stability of the primary motor cortex (M1) when participants performed the trained sequence, while no similar effects were observed for the untrained sequence. Moreover, the increase of MVPC stability correlated with participants' improvement in behavioral performance. These findings suggested that the acquisition of motor skills was supported by the increased connectivity pattern stability between the M1 and the rest of the brain. In summary, our study not only suggests global neural pattern stabilization as a neural signature for effective learning but also advocates applying the MVPC analysis to reveal mechanisms of distributed network reorganization supporting various types of learning. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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8. Comparing mortality and myocardial infarction between coronary artery bypass grafting and drug-eluting stenting in patients with diabetes mellitus and multivessel coronary artery disease: a meta-analysis
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Qi, Xiaolong, Xu, Mingxin, Yang, Haitao, Zhou, Lin, Mao, Yu, Song, Haoming, Li, Quan, and Yang, Changqing
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Systematic review/Meta-analysis ,coronary artery diseases ,surgical procedures, operative ,percutaneous coronary intervention ,coronary artery bypass grafting ,cardiovascular diseases - Abstract
Introduction We aim to compare the midterm outcomes between coronary artery bypass grafting (CABG) and percutaneous coronary intervention (PCI) in diabetic patients who had multivessel coronary artery diseases (CAD). Material and methods A comprehensive literature search was conducted to identify the related clinical studies with a follow-up for 1 year at least. The endpoints were death, myocardial infarction, and major adverse cardiac and cerebrovascular events (MACCE). Results Finally, the analysis of ten studies involving 5,264 patients showed that patients with CABG had worse baseline characteristics, a higher rate of stable angina pectoris, a higher percentage of triple-vessel disease, higher incidence of chronic total occlusion and a higher SYNTAX score. However, there was no significant difference in mortality between the two groups. Additionally, the rates of myocardial infarction and MACCE were markedly decreased in the CABG group. Conclusions The strategy of CABG is better than PCI for diabetic patients with multivessel CAD. The CABG can significantly reduce the rates of myocardial infarction and MACCE and is comparable in mortality despite the worse baseline characteristics.
- Published
- 2014
9. Characterization of immune cells and perforin mutations in familiar venous thromboembolism
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Duan, Qianglin, Lv, Wei, Yang, Minjun, Yang, Fan, Zhu, Yongqiang, Kang, Hui, Song, Haoming, Wang, Shengyue, Dong, Hui, and Wang, Lemin
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Original Article - Abstract
Aim: This study was to carry out exome sequencing in a Han Chinese family with venous thromboembolism. Methods: Three venous thromboembolism (VTE) patients and five members from a Han Chinese family were evaluated by exome sequencing. Results: Among the 3 VTE patients, mutations of 2 genes including PRF1 and HTR2A were identified and predicted to be functionally damaged to their encoded proteins. In addition, the PRF1 mutation and the HTR2A mutation identified in our study were absent in 100 non-related controls, indicating that venous thromboembolism has a genetic component. The R357W mutation is located in the membrane attack complex/perforin domain of PRF1 protein, which exists in both the perforin. The steps of killing foreign or pathological antigen cells by NK cells, CD8 +T cells and the membrane attack complex include membrane perforation and release of the granzyme, either of which is abnormal can lead to immune dysfunction. Conclusions: The mutations of immune related genes in familial VTE might provide new understanding of the pathogenesis of familial venous thromboembolism.
- Published
- 2015
10. Relationship between increase of serum homocysteine caused by smoking and oxidative damage in elderly patients with cardiovascular disease
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Chen, Shengfang, Wu, Ping, Zhou, Lin, Shen, Yuqin, Li, Yunjie, and Song, Haoming
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Original Article - Abstract
Background: To investigate the mechanism of smoking on cardiovascular diseases. Material and methods: 576 elderly patients with cardiovascular diseases in stable condition were consecutive recruited, asked about the living habits and smoking history in a way of face to face. Results: Of all the enrolled patients, current smoking rate was 34.8% for males and 3.4% for females. Average smoking quantity was 17 cigarettes per day and incidence of hyperhomocysteinemia was 38.0%. The homocysteine level in current smokers was significantly higher than that in never smokers (P = 0.004); while the serum folic acid and serum superoxide dismutase (SOD) level were significantly lower those in never smokers (P = 0.012; P = 0.004). The daily smoking consumption and the pack-years of smoking were significantly positively correlated with total homocysteine (tHcy) level (P = 0.020; P = 0.003). The reduced serum SOD level might be associated with increased risk of hypertension (P = 0.023), coronary heart disease (P = 0.018), and stroke (P = 0.035). However, the elevated serum tHcy level was not correlated with increased risk of hypertension and coronary heart disease, while may increase the risk of ischemic stroke (P = 0.075). Conclusions: Smoking status is still prevalent among Chinese elderly patients with cardiovascular diseases, which causes the increase of serum tHcy and the decrease of serum folate as well as SOD; smoking consumption per day and pack-years of smoking have indirect effects on tHcy. And decrease of serum SOD is a risk factor for cardiovascular diseases, increase of serum tHcy may be associated with changes of metabolism caused by oxidative damage.
- Published
- 2015
11. Internal relationship between symptomatic venous thromboembolism and risk factors: up-regulation of integrin β1, β2 and β3 levels
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Duan, Qianglin, Wang, Lemin, Yang, Fan, Li, Jue, Song, Yanli, Gong, Zhu, Li, Guiyuan, Song, Haoming, Zhang, Xiaoyu, Shen, Zugang, and Dart, Anthony
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Original Article ,cardiovascular diseases ,equipment and supplies - Abstract
Background: To compare different expression of core proteins among venous thromboembolism (VTE) and those with risk factor groups and analyze the relative risk for VTE after integrating integrin β1, β2 and β3 expression. Methods: A total of 1006 subjects were recruited and divided into VTE group, risk factor groups and control (non- risk factor) group. Flow cytometry was performed to detect the expression of integrin β1, β2 and β3. The relative risk for VTE was evaluated with independent, parallel and serial methods. Results: The expression of integrin β1 increased markedly in VTE patients, and those with risk factors (acute infection, malignancy, and autoimmune diseases), respectively (P < 0.001 or 0.01). The expression of integrin β1 in trauma/surgery group was not significantly different with control group (P > 0.05). The expression of integrin β2 or β3 significantly increased in VTE group, but that in risk factor groups was not significantly increased (P > 0.05). Multivariate analysis revealed the trauma/surgery groups had no significantly increased risk for VTE. Conclusions: VTE group patients have significantly increased expression of integrin β1, β2 and β3, and risk factor groups (acute infection, malignancy and autoimmune disease) have significantly increased expression of integrin β1. The significant increase in integrin β2, β3 expression is a marker differentiating of VTE group patients with other risk factor groups. Trauma/surgery group has no increased expression of integrin β1, β2 and β3 as other risk factors. Thus, that trauma/surgery may be not the “true” risk factor for VTE.
- Published
- 2015
12. Poly-l-lactic acid/amorphous calcium phosphate bioabsorbable stent causes less inflammation than poly-l-lactic acid stent in coronary arteries
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Kong, Lingting, Liu, Wenbo, Yan, Ge, Li, Qiang, Yang, Haiyan, Yu, Fengxia, and Song, Haoming
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Original Article - Abstract
Aim: Poly-l-lactic acid (PLLA) based bioabsorbable stents with or without amorphous calcium phosphate (ACP) were implanted and compared the inflammation in coronary arteries. Methods: 6 PLLA and 6 PLLA/ACP based paclitaxel-eluting stents were randomly implanted into the coronary arteries of 12 healthy mini-pigs. The segments with stent were used to evaluate inflammation score and endothelialization score by hematoxylin-eosin staining. Results: At the 28th day after stent implantation, no in-stent restenosis or stent thrombosis was found in both PLLA and PLLA/ACP group. Histological analysis indicated that the inflammation score in PLLA/ACP group was less than that of in PLLA group (2.20±0.42 vs. 2.80±0.48, P
- Published
- 2014
13. Upconversion luminescence properties of the novel garnet structure CaLaZrGaO:Yb,Er/Tm phosphors.
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Shi, Yurong, Yang, Lixia, Song, Haoming, Bai, Yumei, Li, Chunyang, and Wang, Zhenling
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LUMINESCENCE ,PHOSPHORS ,SOLID state chemistry ,X-ray diffraction ,CHEMICAL synthesis ,ENERGY transfer - Abstract
Yb-Er and Yb-Tm activated novel garnet structure CaLaZrGaO phosphors were synthesized by solid state reaction and its upconversion luminescence properties were investigated. X-ray diffraction patterns confirmed the synthesized samples were indexed to the garnet structure. The upconversion emission spectra revealed that under 980 nm laser excitation, bright green and blue light had been achieved in CaLaZrGaO:Yb,Er and CaLaZrGaO:Yb,Tm phosphors, respectively. Yb acted as sensitizers in the upconversion process and did not show its characteristic emission peak. Strong Er and Tm emission peaks were obtained via energy transfer from Yb. The possible energy transfer processes of Yb to Er and Tm were discussed. The results indicated that Yb-Er and Yb-Tm activated CaLaZrGaO phosphors exhibited excellent upconversion luminescence properties. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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14. Release of Matrix Metalloproteinases-2 and 9 by S-Nitrosylated Caveolin-1 Contributes to Degradation of Extracellular Matrix in tPA-Treated Hypoxic Endothelial Cells.
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Song, Haoming, Cheng, Youjun, Bi, Gang, Zhu, Yihui, Jun, Wei, Ma, Wenlin, and Wu, Huimin
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MATRIX metalloproteinases , *NITROSYLATION , *CAVEOLINS , *BIODEGRADATION , *EXTRACELLULAR matrix , *HYPOXEMIA , *ENDOTHELIAL cells - Abstract
Intracranial hemorrhage remains the most feared complication in tissue plasminogen activator (tPA) thrombolysis for ischemic stroke. However, the underlying molecular mechanisms are still poorly elucidated. In this study, we reported an important role of caveolin-1 (Cav-1) s-nitrosylation in matrix metalloproteinase (MMP)-2 and 9 secretion from tPA-treated ischemic endothelial cells. Brain vascular endothelial cells (bEND3) were exposed to oxygen-glucose deprivation (OGD) for 2 h before adding recombinant human tPA for 6 h. This treatment induced a significant increase of MMP2 and 9 in the media of bEND3 cells and a simultaneous degradation of fibronectin and laminin β-1, the two main components of extracellular matrix (ECM). Inhibition of MMP2 and 9 with SB-3CT completely blocked the degradation of fibronectin and laminin β-1. ODG+tPA treatment led to Cav-1 shedding from bEND3 cells into the media. Notably, OGD triggered nitric oxide (NO) production and S-nitrosylationof Cav-1 (SNCav-1). Meanwhile tPA induced activation of ERK signal pathway and stimulates the secretion of SNCav-1. Pretreatment of bEND3 cells with C-PTIO (a NO scavenger) or U0126 (a specific ERK inhibitor) significantly reduced OGD-induced S-nitrosylation of Cav-1 in cells and blocked the secretion of Cav-1 and MMP2 and 9 into the media as well as the degradation of fibronectin and laminin β-1 in OGD and tPA-treated cells. These data indicate that OGD-triggered Cav-1 S-nitrosylation interacts with tPA-induced ERK activation to augment MMP2 and 9 secretion and subsequent ECM degradation, which may account for the exacerbation of ischemic blood brain barrier damage following tPA thrombolysis for ischemic stroke. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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15. The Prognostic Value of Peak Cardiac Power Output in Chinese Patients with Chronic Heart Failure.
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Shen, Yuqin, Song, Haoming, Ma, Wenlin, Gong, Zhu, Ni, Yi, Zhang, Xiaoyu, Xu, Wenjun, Jiang, Jinfa, Che, Lin, Xu, Jiahong, Yan, Wenwen, Zhou, Lin, Li, Guanghe, Zhang, Qiping, and Wang, Lemin
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CARDIAC output , *HEART failure , *EXERCISE , *OXYGEN consumption , *ETIOLOGY of diseases ,HEALTH of Chinese people - Abstract
Background: Cardiopulmonary exercise testing has been widely used to risk stratify patients with chronic heart failure (CHF). Peak oxygen consumption (peakVO2) was regarded as a powerful predictor of survival, as it is a surrogate for peak cardiac output (CO), which by most is considered the “true” measure of heart failure. Therefore, it is reasonable to hypothesize that CO is an even stronger predictor than peak VO2. The present study is aimed to investigate the prognostic value of peak cardiac power output (peak CPO) in comparison with peakVO2 in Chinese patients with CHF. Methods: Participants provided written informed consent to participate in this study. Totally 129 patients with CHF underwent symptom-limited cardiopulmonary exercise testing (CPET), with mean age 59.1±11.4 years, 87.6% male, 57.4% ischemic etiology, body mass index (BMI) 24.7±3.7 kg/m2 and LVEF 38±9%. CO was measured using an inert gas rebreathing method. The primary endpoints are cardiac deaths. Results: Over median 33.7-month follow-up, 19 cardiac deaths were reported. Among peak VO2,VE/VCO2 slope and Peak CPO, their area under ROC were 0.64, 0.67, 0.68, respectively (Ρ<0.05).The optimal thresholds for predicting cardiac deaths were peak VO2≤13.4 ml.kg-1.min-1, and VE/VCO2 slope≥39.3 and peak CPO≤ 1.1 respectively by ROC analysis. Finally, in patients with a peak VO2≤13.4 ml.kg-1.min-1 those with peak CPO>1.1W had better survival than those with peak CPO ≤ 1.1W. However, by multivariate analysis adjusted for age, sex, BMI, resting heart rate, LVMI, LVEF, Peak CPO was not an independent predictor of cardiac deaths (P> 0.05). Conclusions: Peak CPO was not a predictor of cardiac death in Chinese CHF patients. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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16. The High Expression of Adhering and Circulating Integrin Serves as a Diagnostic Marker in Venous Thromboembolism.
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Yang, Fan, Song, Yanli, Song, Haoming, Zheng, Weiping, and Wang, Lemin
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THROMBOEMBOLISM , *ENZYME-linked immunosorbent assay , *VENOUS thrombosis , *BLOOD platelet aggregation - Abstract
Objective. To investigate the diagnostic value of circulating integrins β1, 2, and 3 in venous thrombosis (VTE). Materials and Methods. A total of 474 VTE patients and 306 patients with nonhigh risk for VTE as the control group were studied. Levels of adhering integrins β1, 2, and 3 were detected by flow cytometry. Levels of circulating integrins β1, 2, and 3 in serum were measured by enzyme-linked immunosorbent assay. Results. We found that integrins β1, 2, and 3 were expressed highly both in serum and on the surface of leukocytes and platelets in venous thromboembolism. The levels of circulating integrins β1, 2, and 3 are positively correlated with adhering integrins. It showed excellent clinical diagnostic performance of circulating integrins β1, 2, and 3 in venous thromboembolism. Conclusions. Integrin subunit β can be used as a diagnostic marker with high sensitivity and specificity for venous thromboembolism. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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17. Prevention of bleomycin-induced pulmonary fibrosis by vaccination with the Tocilizumab mimotope.
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Guo J, Yang L, Song H, and Bai L
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- Animals, Interleukin-6, Bleomycin adverse effects, Bleomycin metabolism, Lung pathology, Vaccination, Pulmonary Fibrosis chemically induced, Pulmonary Fibrosis prevention & control, Antibodies, Monoclonal, Humanized
- Abstract
Mimotope, a kind of peptide vaccine, is developed to bind natural receptor and inhibit the downstream signaling. We have demonstrated that the vaccination of Tocilizumab mimotopes could alleviate the renal fibrosis by interfering with both IL-6 and ferroptosis signaling. However, the effect of the vaccination of Tocilizumab mimotopes on the fibroblast was not investigated in previous study. Thus, we sought to explore the changes in the fibroblast induced by the Tocilizumab mimotopes vaccination. Bleomycin instillation was performed to construct the pulmonary fibrosis model after the immunization of Tocilizumab mimotopes. Lung histological analysis showed that the Tocilizumab mimotopes could significantly reduce the maladaptive repairment and abnormal remodeling. Immunoblotting assay and fluorescence staining showed that Immunization with the Tocilizumab mimotopes reduces the accumulation of fibrosis-related proteins. High level of lipid peroxidation product was observed in the animal model, while the Tocilizumab mimotopes vaccination could reduce the generation of lipid peroxidation product. Mechanism analysis further showed that Nrf-2 signaling, but not GPX-4 and FSP-1 signaling, was upregulated, and reduced the lipid peroxidation. Our results revealed that in the BLM-induced pulmonary fibrosis, high level of lipid peroxidation product was significantly accumulation in the lung tissues, which might lead to the occurrence of ferroptosis. The IL-6 pathway block therapy could inhibit lipid peroxidation product generation in the lung tissues by upregulating the Nrf-2 signaling, and further alleviate the pulmonary fibrosis.
- Published
- 2024
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18. MiR-155 acts as an inhibitory factor in atherosclerosis-associated arterial pathogenesis by down-regulating NoxA1 related signaling pathway in ApoE -/- mouse.
- Author
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Tang Y, Song H, Shen Y, Yao Y, Yu Y, Wei G, Long B, and Yan W
- Abstract
Background: To investigate the protective efficacy of miR-155 on down regulating NADPH oxidase isoform subunit A1 (NoxA1) gene expression, resulting in inhibition of VSMC migration and over proliferation and thus ameliorating the progression of arterial atherosclerosis in AS mouse model. Therefore, to further explore the regulatory effect of miR-155 on neointima formation in AS and locate potential anti-atherosclerosis target., Methods: The mouse vascular aorta smooth muscle cell (MOVAS) was cultured and transfected with recombinant Pad2YFG adenovirus fluorescent vector with miR-155 fragment into 4 groups. Western blotting and RT-PCR were performed to identify the expression of NoxA1 under different circumstances. Fluorescence microscope was applied to observe the transfection rate of miR-155 into adenovirus. Twelve-week fatty food induced atherosclerotic ApoE
-/- mouse model was established as host to accept miR-155 transfected adenovirus transplantation to observe its effect on VSMC in AS progression. Carotid and thoracic artery were extracted at 1 month after dosing. Distribution of miR-155 was quantified via expression levels of protein and RNA to detect NoxA1, Nox1, p47phox and NADPH expression. Immunohistochemistry, fluorescence imaging and other methods were performed in arteries section to compare the thickness of neointima and assess the severity of AS in each group., Results: Luciferase reporter gene assay showed significant expression of miR-155 in mimic group indicating that miR-155 had target binding effect with NoxA1 gene. Western blotting and RT-PCR results both showed significantly decreased NoxA1 expression in miR-155 mimic group while increased with its inhibitor. The miR-155 distribution was observed varied at 1 month after in control, miR-155 mimic and inhibitor groups. The NoxA1, NADPH, Nox1 and pp47phox protein expression in VSMC was decreased in mimic group vs control and inhibitor groups (P<0.05); no significant difference of NADPH expression was observed in all groups. The NoxA1, Nox1 and p47phox gene expression in VSMC were both found reduced compared with those of control group at week 4 (P<0.05). Immunohistochemistry staining of artery frozen sections figured out that the thickness of neointima of carotid artery in miR-155 mimic group was significantly lower vs control and inhibitor groups (P<0.01) at week 4., Conclusions: miR-155 played an important role in NoxA1-related signaling pathway. miR-155 transfection into VSMC may have anti-inflammatory regulatory effect on NoxA1 expression in vivo and resulting in amelioration of atherosclerotic lesion in AS mouse model. In summary, miR-155 specifically plays in a negative feedback loop and demonstrates a protective role during atherosclerosis-associated VSMC proliferation and neointima formation through the miR-155-NoxA1-p47phox complex signaling pathway., Competing Interests: Conflicts of Interest: All authors have completed the ICMJE uniform disclosure form (available at http://dx.doi.org/10.21037/cdt-20-518). The authors have no conflicts of interest to declare., (2021 Cardiovascular Diagnosis and Therapy. All rights reserved.)- Published
- 2021
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19. Immune Cell Repertoire and Their Mediators in Patients with Acute Myocardial Infarction or Stable Angina Pectoris.
- Author
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Yan W, Song Y, Zhou L, Jiang J, Yang F, Duan Q, Che L, Shen Y, Song H, and Wang L
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- Adaptive Immunity immunology, Adaptive Immunity physiology, Aged, Angina, Stable blood, CD4-Positive T-Lymphocytes immunology, CD8-Positive T-Lymphocytes immunology, Female, Humans, Interferon-gamma blood, Interleukin-2 blood, Interleukin-4 blood, Interleukin-6 blood, Male, Middle Aged, Myocardial Infarction blood, Angina, Stable immunology, Myocardial Infarction immunology
- Abstract
Background: To evaluate the natural innate and adaptive immunity through gene expression and cytology levels in peripheral blood mononuclear cells in patients with acute myocardial infarction (AMI), stable angina pectoris (SAP) and controls. Methods : 210 patients with AMI, 210 with SAP, and 250 clinical controls were recruited. Whole human genome microarray analysis was performed in 20 randomly chosen subjects per group were examined to detect the expressions of complement markers, natural killer cells, T cells and B cells. The quantity of these cells and related cytokines as well as immunoglobulin levels were measured in all subjects. Results: In AMI group, the mRNA expressions of late complement component, markers of natural killer cells, CD3+, CD8+ T cells and B cells were down-regulated, while those of early complement component and CD4+T cells were up-regulated (p<0.05). In both AMI and SAP patients, the quantity of natural killer cells, CD3+, CD8+ T cells, B cells, IgM and IgG were significantly lower than those of the controls. CD4+ T cells, CH50, C3, C4, IL-2, IL-4, IL-6 and IFN-γ were significantly higher (p<0.05). Conclusions : In AMI patients, both of gene expressions related to complement, natural killer cells, CD3+, CD8+ T cells, B cells and the quantity of these immune cells decreased while cell number reduced in SAP patients. Immune function in both AMI and SAP patients decreased especially in AMI patients with declined gene and protein levels. To improve the immune system is a potential target for medical interventions and prevention in AMI., Competing Interests: Competing Interests: The authors have declared that no competing interest exists.
- Published
- 2017
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20. VE/VCO 2 slope and its prognostic value in patients with chronic heart failure.
- Author
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Shen Y, Zhang X, Ma W, Song H, Gong Z, Wang Q, Che L, Xu W, Jiang J, Xu J, Yan W, Zhou L, Ni YI, Li G, Zhang Q, and Wang L
- Abstract
The minute ventilation/carbon dioxide production (VE/VCO
2 ) slope has been widely demonstrated to have strong prognostic value in patients with chronic heart failure (CHF), and the risk of mortality is believed to increase when the VE/VCO2 slope is >32.8; however, there is little evidence concerning the prognostic value of the VE/VCO2 slope in Chinese patients. In the present study, the prognostic value of the VE/VCO2 slope was investigated in patients with CHF. A total of 258 subjects underwent symptom-limited cardiopulmonary exercise testing (CPET) and were divided into CHF (113 males and 16 females; LVEF <0.49) and control (106 males and 23 females) groups. The cardiac-related events over a median 33.7-month follow-up period subsequent to the CPET were evaluated using receiver operating characteristic curve analysis. The VE/VCO2 slope was significantly different between the CHF and control groups (P<0.001). The area under the curve (AUC) for the VE/VCO2 slope in predicting cardiac-related mortalities in the patients with CHF was 0.670 (P<0.05), and the sensitivity and specificity of the VE/VCO2 slope were 0.667 and 0.620, respectively. The optimal threshold of the VE/VCO2 slope for predicting cardiac-related mortalities in patients with CHF was ≥39.3. The AUC for the VE/VCO2 slope in predicting cardiac-related hospitalizations in patients with CHF was 0.682 (P<0.05), and the sensitivity and specificity of the VE/VCO2 slope were 0.631 and 0.778, respectively. The optimal threshold of the VE/VCO2 slope for predicting cardiac-related hospitalizations in patients with CHF was ≥32.9. In conclusion, ventilatory efficiency decreases in patients with CHF. The VE/VCO2 slope is a strong predictor of cardiac-related mortalities in the patients with CHF analyzed.- Published
- 2015
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21. Serum gamma-glutamyl transferase: a novel biomarker for coronary artery disease.
- Author
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Mao Y, Qi X, Xu W, Song H, Xu M, Ma W, and Zhou L
- Subjects
- Adult, Biomarkers blood, Coronary Angiography, Coronary Artery Disease diagnostic imaging, Female, Humans, Male, Middle Aged, Multivariate Analysis, Risk Factors, Coronary Artery Disease blood, Coronary Artery Disease enzymology, gamma-Glutamyltransferase blood
- Abstract
Background: Atherosclerosis is a chronic inflammatory process, in which oxidative stress is the key event. Gamma-glutamyl transferase (GGT) is a cellular production of oxidants. We aimed to elucidate the relationship of serum GGT levels and coronary artery disease (CAD) in a Chinese population., Material and Methods: A total of 513 adult subjects who had undergone coronary angiography were enrolled in the study. Clinical characteristics, coronary angiography, and serum samples were collected from all the patients and analyzed for the serum GGT, blood lipids, and cardiovascular risk factors., Results: Subjects with CAD had significantly increased activity of serum GGT (p=0.003). Serum GGT levels exhibited positive correlations with alcohol intake (β=0.177, p<0.001), coronary complexity (β=0.068, p<0.001), and triacylglycerol (β=0.058, p<0.001). High-density lipoprotein cholesterol levels (β=0.157, p=0.008) and age (β=0.004, p=0.002) were negatively correlated with serum GGT in the CAD group. The coronary complexity presented a negative correlation with Ig-apolipoprotein AI (β=-2.517, p=0.001) and positive correlations with smoking (β=0.640, p<0.001), Ig-GGT (β=0.613, p=0.004), Ig high sensitivity-C reactive protein (β=0.320, p<0.001), and hypertension (β=0.286, p<0.026)., Conclusions: The study showed a positive correlation between serum GGT and CAD in a Chinese population. Serum GGT levels may be a potential biomarker for CAD.
- Published
- 2014
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22. Symptomatic venous thromboembolism is a disease related to infection and immune dysfunction.
- Author
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Duan Q, Gong Z, Song H, Wang L, Yang F, Lv W, and Song Y
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- Antigens, CD19 genetics, CD3 Complex genetics, CD4-CD8 Ratio, CD56 Antigen genetics, Humans, Killer Cells, Natural metabolism, Receptors, IgG genetics, T-Lymphocyte Subsets metabolism, Oligonucleotide Array Sequence Analysis methods, Venous Thromboembolism genetics, Venous Thromboembolism immunology
- Abstract
The characteristics of human genomics and cellular immune function between clinically symptomatic venous thromboembolism (VTE) and controls were systematically compared to explore the immunologic pathogenesis of VTE. Microarray assay showed the mRNA expressions of genes related to non-specific cellarer immune and cytokines were significantly down-regulated. Abnormal expressions of CD3+, CD4+, CD8+, NK marker CD16+56+, CD19 and aberrant CD4+/CD8+ ratio were detected in 54 among 56 patients. In PE patients, microarray assay revealed the imbalance in the expressions of genes related to the immune system. The expressions of genes related to non-specific immune cells and cytokines were markedly up-regulated and those associated with cellular immune were dramatically down-regulated. In VTE patients, cytological examination indicated the functions of NK cells were significantly compromised, and the antigen recognition and killing function of T cells markedly decreased. The consistence between genomic and cytological examination suggests the symptomatic VTE is closely associated with the infection and immune dysfunction.
- Published
- 2012
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23. Analysis on the pathogenesis of symptomatic pulmonary embolism with human genomics.
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Wang H, Duan Q, Wang L, Gong Z, Liang A, Wang Q, Song H, Yang F, and Song Y
- Subjects
- Adult, Aged, Aged, 80 and over, Female, Humans, Male, Middle Aged, Genomics methods, Pulmonary Embolism genetics, Pulmonary Embolism pathology
- Abstract
Background: In the present study, the whole human genome oligo microarray was employed to investigate the gene expression profile in symptomatic pulmonary embolism (PE)., Methods: Twenty patients with PE and 20 age and gender matched patients without PE as controls were enrolled into the present study in the same period. The diagnosis of PE was based on the clinical manifestations and findings on imaging examinations. Acute arterial and/or venous thrombosis was excluded in controls. The whole human genome oligo microarray was employed for detection. Statistical analysis was performed with t test following analysis of very small samples of repeated measurements and Gene Ontology (GO) analysis., Results: Genomic data showed no damage to vascular endothelial cells in PE patients. Genomic data only found increased mRNA expression of a small amount of coagulation factors in PE patients. In the PE group, anticoagulant proteins, Fibrinolytic system and proteins related to platelet functions only played partial roles in the pathogenesis of PE. In addition, the mRNA expressions of a fraction of adhesion molecules were markedly up-regulated. Gene Ontology analysis showed the genes with down-regulated expressions mainly explain the compromised T cell immunity. Symptomatic VTE patients have compromised T cell immunity., Conclusion: The damage to vascular endothelial cells is not necessary in the pathogenesis of VTE, and only a fraction of factors involved in the shared coagulation cascade are activated. Genomic results may provide a new clue for clinical diagnosis, treatment and prevention of VTE.
- Published
- 2012
- Full Text
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