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Apple pomace is a by-product from the apple processing industry and can be used for the production of many value-added compounds such as enzymes, proteins, and nutraceuticals, among others. An investigation was carried out to study the improvement in the protein content in apple pomace by solid-state fermentation using the fungus Phanerochaete chrysosporium by tray fermentation method. The effect of this protein in terms of how it enriched apple pomace as animal feed for pigs has also been studied. There was a 36% increase in protein content in the experimental diet with 5% w/w fermented apple pomace. The efficiency of conversion of ingested food was increased from 43.5 ± 2.5 to 83.1 ± 4.4 in the control group and the efficiency of conversion of feed increased from 55.4 ± 4.5 to 92.1 ± 3.6 in the experimental group during the animal feed experiment. Similarly, the effect of a protein enriched diet on odor emission and greenhouse gas emission has also been studied. The results demonstrated that the protein enrichment of apple pomace by solid state cultivation of the fungus P. chrysosporium makes it possible to use it as a dietary supplement for pigs.

Biobutanol has conventionally been generated by fermentation of carbohydrates derived from biomass (starch or sugar-based feedstock, such as corn) using Clostridia strains (mainly C. beijerinckii and C. acetobutylicum) under anaerobic conditions in batch mode. Under these premises, it has been tough for the acetone-butanol-ethanol fermentation to compete with petro-butanol production from an energy efficiency and material consumption standpoint. Challenges for butanol production from biomass comprised high cost of feedstock, scarcity of hyper-butanol producing bacteria and low butanol yield, volumetric productivity and titre, leading to high water usage and separation-purification costs. This article is an up-to-date review on several under explored sections, such as optimization of fermenter feed, microbial culture responsible for solvent production (co-culture techniques and electro-biochemical process), latest recovery techniques and the studies integrating in situ continuous fermentation processes. Biobutanol refinery way forward should build upon the use of low-cost lignocellulosic matter and zero cost organic wastes and by-products from food, agriculture, forestry, fermentation and paper industries as feedstock; optimized fermentation of such diversified feed with appropriate hyper-butanol producing strains in biofilm reactors and integration of fermentation step with hybrid high butanol-selective recovery techniques. Copyright © 2015 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]

Abstract: Conidiation and lytic enzyme production by Trichoderma viride at different solids concentration of pre-treated municipal wastewater sludge was examined in a 15-L fermenter. The maximum conidia concentration (5.94×107 CFUmL−1 at 96h) was obtained at 30gL−1 suspended solids. The maximum lytic enzyme activities were achieved around 12–30h of fermentation. Bioassay against a fungal phytopathogen, Fusarium sp. showed maximum activity in the sample drawn around 96h of fermentation at 30gL−1 suspended solids concentration. Entomotoxicity against spruce budworm larvae showed maximum value ≈17290SBUμL−1 at 30gL−1 suspended solids concentration at the end of fermentation (96h). Plant bioassay showed dual action of T. viride, i.e., disease prevention and growth promotion. The rheological analyses of fermentation sludges showed the pseudoplastic behaviour. In order to maintain required dissolved oxygen concentration ≥30%, the agitation and aeration requirements significantly increased at 35gL−1 compared to 30 and 25gL−1. The oxygen uptake rate and volumetric oxygen mass transfer coefficient, k L a at 35gL−1 did not increase in comparison to 30gL−1 due to rheological complexity of the broth during fermentation. Thus, the successful fermentation operation of the biocontrol fungus T. viride is a rational indication of its potential for mass-scale production for agriculture and forest sector as a biocontrol agent. [Copyright &y& Elsevier]

Pulp and paper solid waste (PPSW) originating from paper industry, was experimented for the production of fumaric acid (FA) through submerged and solid state fermentation by utilizing the filamentous fungus Rhizopus oryzae 1526 . Physicochemical characterization, pH and moisture content analysis of PPSW was carried out. Pre-treatment of PPSW by size reduction resulted in particles of different size ranges (1.7 mm < x ≤ 3.35 mm, 850 μm < x ≤ 1.7 mm, 300 μm < x ≤ 850 μm, 75 μm < x ≤ 300 μm and 33 μm < x ≤ 75 μm). In submerged fermentation with all particle size ranges, a maximum of 23.47 ± 0.70 g/L of FA was obtained with 33 μm < x ≤ 75 μm under the fermentation conditions of 30 °C, 200 rpm, 5% pre-cultured inoculum ( v/v ) and at 48 h. Viscosity measurement and analysis of by-product of the fermented broths were performed. Microwave-phosphoric acid mediated hydrolysis of 33 μm < x ≤ 75 μm particle size produced hydrolysate with maximum glucose (11.2 ± 0.8 g/L) and xylose (20.22 ± 0.85 g/L) contents. Submerged fermentation with this hydrolysate confirmed the utilization of xylose for both FA production and fungal growth. Solid state fermentation with 75 μm < x ≤ 300 μm particle size resulted in highest FA production (41.45 g/kg dry weight) after 21 days. Scanning electron microscopy revealed the morphological features of the fungus grown on the particles. The results of the present study confirmed the utilization of PPSW as a source of carbon and trace elements by the fungus R. oryzae 1526 and also the bioconversion into FA during fermentation. FA being a high value platform chemical, its bioproduction from the low cost PPSW, is a value addition approach. [ABSTRACT FROM AUTHOR]

The paper discusses the potential of different waste biomaterials for biosorption. Waste biomaterials after citric acid fermentation and chitosan extraction using Aspergillus niger were evaluated for biosorption of toxic metals (Cu, Cr and As) from leachates of chromated copper arsenate woods. The different waste BMs, such as fungal biomass (living and dead), alkali insoluble material and acid and alkali insoluble material were used in this study. The effect of different parameters, such as biosorbent concentration, metal concentration and contact time were investigated. The fitness of biosorption data for Freundlich and Langmuir adsorption models was investigated through batch adsorption technique. Among the adsorption isotherm tested, Langmuir isotherm gave the best fit with correlation coefficients (R 2 ) value ranging from 0.89 to 0.97; 0.96–0.99 and 0.76–0.95 for As, Cr and Cu, respectively using solid state fermented biomass. Similarly, the significant removal of metals (>60% in leachate 2) from waste CCA wood leachate was achieved with the different BMs. Therefore, this study demonstrates the potential of CA fermentation derived waste BMs for biosorption of toxic metals from waste waters. [ABSTRACT FROM AUTHOR]

Butanol produced from renewable feedstock is defined as an emerging biofuel and biochemical. Research efforts made during the last three decades on biochemical production of butanol via conventional ABE (acetone-ethanol-butanol) fermentation has tried to bring biobutanol close to competition with petrobutanol. However, each new effort of development has been often countered by new challenges, confining biobutanol production mostly to the laboratory scale. This review provides a systematic, comparative analysis of different steps in biochemical production of butanol and identifies the counteractive aspects and challenges to overcome. A special emphasis is given on process inhibitors, applied detoxification techniques, chemical supplements and research & development in industry in order to enhance and update ABE fermentation and make it cost effective. Biobutanol future lies in utilization of inexpensive cellulose enriched lignocellulosic hydrolysates and hyper-butanol producing bacteria, combined with specific detoxification techniques and followed by efficient continuous fermentation technologies together with in situ product recovery. [ABSTRACT FROM AUTHOR]