104 results on '"Zhang, Mingqing"'
Search Results
2. Per- and polyfluoroalkyl substances in Chinese surface waters: A review
- Author
-
Zhang, Lucheng, Wang, Minyue, Zhang, Mingqing, and Yang, Dejun
- Published
- 2023
- Full Text
- View/download PDF
3. Transcriptome and hypoxia-responsive gene expression analyses reveal the physiological reaction to acute hypoxia and reoxygenation in Epinephelus coioides
- Author
-
Wu, Yuxin, Lai, Xingxing, Lin, Bing, Lin, Yiran, Yang, Yang, Zhang, Mingqing, Liu, Su, Wu, Jinghui, Fu, Xiaoqin, and Shu, Hu
- Published
- 2023
- Full Text
- View/download PDF
4. Functional genomic characterization unveils probiotic features of Bacillus cereus G1-11 isolated from the gut of the hybrid grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂)
- Author
-
Xie, Guangting, Zhu, Yating, Zhong, Zhongxuan, Du, Qianping, Wu, Yuxin, Xing, Ke, Zhang, Mingqing, and Shu, Hu
- Published
- 2023
- Full Text
- View/download PDF
5. Long term effects of crop rotation and fertilization on crop yield stability in southeast China
- Author
-
Zhang, Licheng, Yuan, Jie, Zhang, Mingqing, Zhang, Yongchun, Wang, Limin, and Li, Juan
- Published
- 2022
- Full Text
- View/download PDF
6. Democratization Prospects and Challenges in Central and Eastern Europe after the Cold War: A Focus on the Visegrad Group in the Context of European Integration
- Author
-
Zhang Longtai and Zhang Mingqing
- Subjects
Social Sciences - Abstract
This research focuses on democratization and consolidation in Central and Eastern Europe, specifically examining the Visegrad Group (Poland, Hungary, Slovakia, and the Czech Republic) after the Cold War and EU Eastern Enlargement. Using different democratization and European Integration theories, it explores the factors influencing democratization, consolidation, and stability in this region. This study analyzes the transition from communism to democracy, the impact of European Integration on democratization, and the role of civil society organizations in promoting democratic values. It acknowledges the challenges posed by populism and illiberalism and suggests practical policy solutions to strengthen democratic values and stability in Central and Eastern Europe.
- Published
- 2023
- Full Text
- View/download PDF
7. Trends in colorectal cancer screening compliance and incidence among 60‐ to 74‐year‐olds in China.
- Author
-
Zhang, Mingqing, Zhang, Yongdan, Guo, Lu, Zhao, Lizhong, Jing, Haoren, Yang, Xiao, Zhang, Wen, Zhang, Yong, Nie, Zhenguo, Zhu, Siwei, Zhang, Shiwu, and Zhang, Xipeng
- Subjects
- *
EARLY detection of cancer , *COLORECTAL cancer , *OLDER people , *LOGISTIC regression analysis , *ADENOMA - Abstract
Background: Compliance with colonoscopy among elderly individuals participating in colorectal cancer (CRC) screening programs is unsatisfactory, despite a high detection rate of bowel‐related diseases. In this study, our aim was to analyze the impact of risk factors on the trends of compliance and detection rates in colonoscopy among high‐risk individuals aged 60–74. Methods: A retrospective study was conducted on the high‐risk individuals aged 60–74 participating in the 2021 CRC screening program in Tianjin, China. Logistic regression analyses, including both univariate and multivariate analyses, were performed to explore the impact of different risk factors on colonoscopy compliance among the high‐risk individuals. Besides, the study investigated the influence of various risk factors on the detection rates of bowel‐related diseases among the high‐risk individuals who underwent colonoscopy. Results: A total of 24,064 high‐risk individuals were included, and 5478 individuals received a free colonoscopy, with an overall compliance of 22.76%. Among them, the adenoma detection rate was 55.46%. Males and individuals with a positive FIT had high compliance and detection rates for CRC, advanced adenomas (AA), advanced colorectal neoplasia (ACN), and colorectal neoplasm (CN). Individuals aged 70–74 were associated with low compliance but high CRC, ACN, and CN detection rates. Individuals who reported a history of chronic constipation, bloody mucous, and CRC in first‐degree relative showed high compliance but no significantwere associated with the detection rates of CRC, AA, and CN. Conclusion: This study reported several risk factors associated with the screening behaviors for CRC. Patterns and trends in CRC, AA, ACN, and CN compliance and detection rates correlate with risk factors. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
8. Premedication with reformulated simethicone and sodium bicarbonate improves mucosal visibility during upper gastrointestinal endoscopy: a double-blind, multicenter, randomized controlled trial
- Author
-
Chen, Xueqin, Dai, Ning, Deng, Yanyong, Sun, Xin, Zhang, Mingqing, Pan, Jie, Huang, Zhiming, Ye, Guoliang, Si, Jianmin, Wang, Lan, and Chen, Shujie
- Published
- 2021
- Full Text
- View/download PDF
9. Application of ultrasound-guided intranodal lymphangiography and embolisation in cancer patients with postoperative lymphatic leakage
- Author
-
Sun, Xingwei, Zhou, Feng, Bai, Xuming, Yuan, Qiang, Zhang, Mingqing, Ma, Liang, and Jin, Yong
- Published
- 2021
- Full Text
- View/download PDF
10. Interface Engineering to Drive High‐Performance MXene/PbS Quantum Dot NIR Photodiode.
- Author
-
Di, Yunxiang, Ba, Kun, Chen, Yan, Wang, Xudong, Zhang, Mingqing, Huang, Xinning, Long, Yi, Liu, Mengdi, Zhang, Shukui, Tang, Weiyi, Huang, Zhangcheng, Lin, Tie, Shen, Hong, Meng, Xiangjian, Han, Meikang, Liu, Qi, and Wang, Jianlu
- Subjects
QUANTUM dots ,SEMICONDUCTOR nanocrystals ,INDIUM oxide ,INDIUM tin oxide ,OPTOELECTRONIC devices ,LEAD sulfide ,ELECTRIC conductivity - Abstract
The realization of a controllable transparent conducting system with selective light transparency is crucial for exploring many of the most intriguing effects in top‐illuminated optoelectronic devices. However, the performance is limited by insufficient electrical conductivity, low work function, and vulnerable interface of traditional transparent conducting materials, such as tin‐doped indium oxide. Here, it is reported that two‐dimensional (2D) titanium carbide (Ti3C2Tx) MXene film acts as an efficient transparent conducting electrode for the lead sulfide (PbS) colloidal quantum dots (CQDs) photodiode with controllable near infrared transmittance. The solution‐processed interface engineering of MXene and PbS layers remarkably reduces the interface defects of MXene/PbS CQDs and the carrier concentration in the PbS layer. The stable Ti3C2Tx/PbS CQDs photodiodes give rise to a high specific detectivity of 5.51 × 1012 cm W−1 Hz1/2, a large dynamic response range of 140 dB, and a large bandwidth of 0.76 MHz at 940 nm in the self‐powered state, ranking among the most exceptional in terms of comprehensive performance among reported PbS CQDs photodiodes. In contrast with the traditional photodiode technologies, this efficient and stable approach opens a new horizon to construct widely used infrared photodiodes with CQDs and MXenes. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
11. The Surgical Timing and Prognoses of Elderly Patients with Hip Fractures: A Retrospective Analysis
- Author
-
Sun,Lili, Wang,Cong, Zhang,Mingqing, Li,Xiang, and Zhao,Bin
- Subjects
Clinical Interventions in Aging - Abstract
Lili Sun, Cong Wang, Mingqing Zhang, Xiang Li, Bin Zhao Department of Emergency, Beijing Jishuitan Hospital, Beijing, Peopleâs Republic of ChinaCorrespondence: Bin Zhao, Department of Emergency, Beijing Jishuitan Hospital, No. 31 of Xinjiekou East Street, Xicheng District, Beijing, 100035, Peopleâs Republic of China, Tel +86-1058516417, Fax +86-1058516931, Email binzhao1492@163.comBackground: Guidelines exist for the surgical treatment of hip fractures, but the association between the surgical timing and the incidence of postoperative complications and other important outcomes in elderly patients with hip fracture remains controversial.Objective: This study aims to explore the association between the surgical timing and the prognoses in elderly patients with hip fracture.Methods: A total of 701 elderly patients (age ⥠65 years) with hip fractures who were treated in our hospital from June 2020 to June 2021 were selected. Patients who underwent surgery within 2 d of admission were assigned to the early surgery group, and those who underwent surgery after 2 d of admission were assigned to the delayed surgery group. The prognosis indices of the patients in the two groups were recorded and compared.Results: The length of postoperative hospitalisation in the early surgery group was significantly lower than that in the delayed surgery group (P < 0.001). The European quality of life questionnaire (EQ-5D) utility in the delayed surgery group was significantly lower than that in the early surgery group at 30 days and 6 months after operation (P< 0.05). Compared with the delayed surgery group, the incidence of pulmonary infection, urinary tract infection (UTI) and deep vein thrombosis (DVT) in the early surgery group were significantly lower. There were no significant differences between the two groups in terms of mortality and excellent rates of the HHS at six months after the operation. In addition, the early surgery group had a lower readmission rate than the delayed surgery group [34 (9.5%) vs 56 (16.3%), P = 0.008].Conclusion: Earlier surgery can reduce the incidence of pulmonary infections, UTI, DVT and readmission rate among elderly patients with hip fractures, shorten postoperative hospitalisation.Keywords: Hip fracture, elderly patients, timing of surgery, prognosis
- Published
- 2023
12. A Novel Vermiculite/TiO 2 Composite: Synergistic Mechanism of Enhanced Photocatalysis towards Organic Pollutant Removal.
- Author
-
Han, Lin, Yue, Xiaoju, Wen, Liying, Zhang, Mingqing, and Wang, Shifeng
- Subjects
VERMICULITE ,PHOTOCATALYSIS ,HETEROJUNCTIONS ,TITANIUM dioxide ,POLLUTANTS ,PHOTOCATALYSTS ,BAND gaps ,SUPERABSORBENT polymers - Abstract
There has been increasing concern over water pollution, which poses a threat to human life and health. Absorption by low-cost absorbents is considered to be a cost-effective and efficient route. However, the non-reusability of absorbents greatly limits their applications. In this study, a novel vermiculite/TiO
2 composite combining the inexpensive absorbent with the commonly used photocatalyst was firstly synthesized via the sol-gel method. On the one hand, the organic pollutants are absorbed by vermiculite and then decomposed through the photocatalysis process, enabling the next round of absorption and creating an absorption–decomposition reusable cycle. On the other hand, the modulation effect of optical and electronic structure on the prepared TiO2 photocatalyst by the vermiculite incorporation could significantly improve the photocatalytic activity and eventually enhance the aforementioned cyclic degradation capacity. The layer-structured vermiculite (Vt) supports a uniform coverage of TiO2 at an optimized ratio, providing an optimal adsorption environment and contact area between the photocatalyst and methylene blue (MB) molecules. Vt/TiO2 heterojunction is formed with Si-O-Ti bonding, at which electrons transfer from Vt to TiO2 , enriching electron density in TiO2 and favoring its photocatalytic activity. Furthermore, the incorporation of Vt increases the light absorption of TiO2 in the visible range by narrowing the optical band gap to 1.98 eV, which could promote the generation of photo-excited carriers. In addition, PL measurements revealed that the carrier recombination is substantially suppressed, and the charge separation and migration are greatly enhanced by a factor of 3. As a result, the decomposition rate of MB is substantially increased 5.3-fold, which is ascribed to the synergistic effects of the elevated photocatalysis and the large absorption capacity governed by the chemisorption mechanism of the intra-particle diffusion. These results pave the way for composite design towards efficient, economical, and pragmatic water pollution treatment. [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF
13. Extreme phenophase delays and their relationship with natural forcings in Beijing over the past 260 years
- Author
-
Liu, Yang, Zhang, Mingqing, and Fang, Xiuqi
- Published
- 2018
- Full Text
- View/download PDF
14. 5-Fluorouracil Suppresses Colon Tumor through Activating the p53-Fas Pathway to Sensitize Myeloid-Derived Suppressor Cells to FasL + Cytotoxic T Lymphocyte Cytotoxicity.
- Author
-
Yang, Yingcui, Zhang, Mingqing, Zhang, Yongdan, Liu, Kebin, and Lu, Chunwan
- Subjects
- *
COLON tumors , *IN vitro studies , *FLOW cytometry , *IN vivo studies , *SEQUENCE analysis , *ANALYSIS of variance , *CANCER chemotherapy , *ANIMAL experimentation , *WESTERN immunoblotting , *BLOOD collection , *FLUOROURACIL , *MYELOID-derived suppressor cells , *COMPARATIVE studies , *DESCRIPTIVE statistics , *GENE expression profiling , *HISTOLOGICAL techniques , *RESEARCH funding , *TUMOR markers , *T cells , *CELL surface antigens , *CELL lines , *IMMUNODIAGNOSIS , *MICE , *RECOMBINANT proteins , *CELL death - Abstract
Simple Summary: 5-Fluorouracil (5-FU), in combination with various therapeutic agents, is the main strategy for patients with high risk of stage 2, and advanced stages of, human colorectal cancer. Although 5-FU-based chemotherapy causes myeloid cell suppression, which has long been considered as a major adverse effect in certain cancer patients, recent studies determined that 5-FU selectively kills myeloid-derived suppressor cells (MDSCs), to increase cytotoxic T lymphocyte activation. However, the molecular mechanism underlying 5-FU's suppression of MDSCs is incompletely understood. We report here that 5-FU activates p53, to upregulate Fas expression in MDSCs, to increase MDSC sensitivity to FasL-induced apoptosis in vitro. 5-FU therapy upregulates Fas expression, to suppress MDSC accumulation, to increase CTL tumor infiltration in tumor-bearing mice. In human colorectal cancer patients, 5-FU-based chemotherapy suppresses MDSCs and increases CTLs level. Our findings determine that the p53-Fas pathway links 5-FU to MDSC suppression. Myelosuppression is a major adverse effect of 5-fluorouracil (5-FU) chemotherapy. However, recent findings indicate that 5-FU selectively suppresses myeloid-derived suppressor cells (MDSCs), to enhance antitumor immunity in tumor-bearing mice. 5-FU-mediated myelosuppression may thus have a beneficial effect for cancer patients. The molecular mechanism underlying 5-FU's suppression of MDSCs is currently unknown. We aimed at testing the hypothesis that 5-FU suppresses MDSCs through enhancing MDSC sensitivity to Fas-mediated apoptosis. We observed that, although FasL is highly expressed in T cells, Fas is weakly expressed in myeloid cells in human colon carcinoma, indicating that downregulation of Fas is a mechanism underlying myeloid cell survival and accumulation in human colon cancer. 5-FU treatment upregulated expression of both p53 and Fas, and knocking down p53 diminished 5-FU-induced Fas expression in MDSC-like cells, in vitro. 5-FU treatment also increased MDSC-like cell sensitivity to FasL-induced apoptosis in vitro. Furthermore, we determined that 5-FU therapy increased expression of Fas on MDSCs, suppressed MDSC accumulation, and increased CTL tumor infiltration in colon tumor-bearing mice. In human colorectal cancer patients, 5-FU chemotherapy decreased MDSC accumulation and increased CTL level. Our findings determine that 5-FU chemotherapy activates the p53-Fas pathway, to suppress MDSC accumulation, to increase CTL tumor infiltration. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
15. Improving Rice Modeling Success Rate with Ternary Non-structural Fertilizer Response Model
- Author
-
Li, Juan, Zhang, Mingqing, Chen, Fang, and Yao, Baoquan
- Published
- 2018
- Full Text
- View/download PDF
16. Endoscopic Transcecal Appendectomy With a Novel Detachable Over-the-Scope Clip
- Author
-
Guan, Fu, Zhang, Mingqing, and Wang, Shengbing
- Published
- 2024
- Full Text
- View/download PDF
17. The Prognostic Value and the Oncogenic and Immunological Roles of Vacuolar Protein Sorting Associated Protein 26 A in Pancreatic Adenocarcinoma.
- Author
-
Hou, Jihuan, Wu, Han, Xu, Beibei, Shang, Jin, Xu, Xuechun, Li, Guixia, Zhang, Haoran, Zhang, Wenqing, Deng, Yabin, Hong, Xiaoting, Hu, Tianhui, Zhang, Mingqing, and Zhan, Yanyan
- Subjects
PROGNOSIS ,GENE expression ,CANCER prognosis ,REGULATION of growth ,CELLULAR control mechanisms ,CELL adhesion - Abstract
The identification of the prognostic markers and therapeutic targets might benefit the diagnosis and treatment of pancreatic adenocarcinoma (PAAD), one of the most aggressive malignancies. Vacuolar protein sorting associated protein 26 A (VPS26A) is a candidate prognosis gene for hepatocellular carcinoma, but its expression and function in PAAD remain unknown. The mRNA and protein expression of VPS26A in PAAD was explored and validated by bioinformatics and immunohistochemical analysis. The correlation between VPS26A expression and various clinical parameters, genetic status, diagnostic and prognostic value, survival and immune infiltration were evaluated, and the co-expressed gene-set enrichment analysis for VPS26A was performed. Cytologic and molecular experiments were further carried out to investigate the role and potential mechanism of VPS26A in PAAD. The mRNA and protein levels of VPS26A were elevated in PAAD tissues. High VPS26A expression was associated with the advanced histological type, tumor stage simplified, smoking status and tumor mutational burden score, and the poor prognosis of PAAD patients. VPS26A expression was significantly correlated with immune infiltration and immunotherapy response. VPS26A-co-expressed genes were mainly enriched in the regulation of cell adhesion and actin cytoskeleton and the immune-response-regulating signaling pathway. Our experiments further demonstrated that VPS26A promoted the proliferation, migration and invasion potentials of PAAD cell lines through activating the EGFR/ERK signaling. Our study suggested that VPS26A could be a potential biomarker and a therapeutic target for PAAD through comprehensive regulation of its growth, migration and immune microenvironment. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
18. Reply: Request for clarification on symptom assessment methodology in high‐risk population colonoscopy study.
- Author
-
Zhang, Mingqing, Zhang, Yongdan, Zhang, Wen, Zhao, Lizhong, Jing, Haoren, Wu, Xiaojing, Guo, Lu, Zhang, Haixiang, Zhang, Yong, Zhu, Siwei, Zhang, Shiwu, and Zhang, Xipeng
- Subjects
- *
IRRITABLE colon , *HEALTH facilities , *SYMPTOMS , *COLONOSCOPY , *LIFE change events - Abstract
We would like to express our gratitude to Safari, Farzad, and Noursina, Ali, for their insightful comments about our article regarding the questionnaire procedure and the assessment of symptoms, including a history of chronic constipation, chronic diarrhea, and mucous blood stools. This questionnaire has been included in national screening programs since 2007 and has been used in many cities in China.[[1], [3]] Since 2012, CRC screening in community allied third-grade class-A hospitals has been initiated in Tianjin City, China. As stated in the manuscript, only 39.41% of the screening population completed a colonoscopy within 1 month, so those with symptoms at the time of initial screening were advised to seek a definitive diagnosis promptly and were excluded from screening. [Extracted from the article]
- Published
- 2023
- Full Text
- View/download PDF
19. Prognosis of Patients Over 60 Years Old With Early Rectal Cancer Undergoing Transanal Endoscopic Microsurgery – A Single-Center Experience.
- Author
-
Zhang, Mingqing, Zhang, Yongdan, Jing, Haoren, Zhao, Lizhong, Xu, Mingyue, Xu, Hui, Zhu, Siwei, and Zhang, Xipeng
- Subjects
RECTAL cancer ,MICROSURGERY ,OLDER patients ,PRESERVATION of organs, tissues, etc. ,CANCER relapse - Abstract
Aim: Transanal endoscopic microsurgery (TEM) is widely performed in early rectal cancer. This technique offers greater organ preservation and decreases the risk of subsequent surgery. However, postoperative local recurrence and distant metastasis remain challenges for patients with high-risk pathological factors. This single-center study reports the prognosis of early rectal cancer patients over 60 years old after TEM. Methods: The data of the patients over 60 years old who underwent local anal resection were collected retrospectively. Moreover, the 5-year follow-up data were analyzed to determine the 5-year DFS and OS. Results: 47 early rectal cancer patients over 60 years old underwent TEM. There were 27 patients with high-risk factors and 20 patients without high-risk factors. Two patients underwent radical surgery after TEM and ten patients received adjuvant treatment. Local recurrence occurred in 7 patients, of which 4 underwent salvage surgery. The 5-year progression-free survival rate was 75.6%, which was lower in the high-risk patients group (69.6%) than in the non-high-risk patients group (83.3%) (P >0.05). The 5-year OS was 90.2%, but there was no statistically significant difference between the two groups (high-risk patients 87.0%, non-high-risk patients 94.4%). Furthermore, there was no significant difference in DFS and OS between people over and under 70 years old. Conclusion: Some high-risk factor patients over 60 years old do not have inferior 5-year DFS and OS to the non-high-risk patients. TEM is an option for old patients with high surgical risks. Even if postoperative pathology revealed high-risk factors, timely surgical treatment after local recurrence would be beneficial to improve the 5-year DFS and OS. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
20. Construction of Bronze TiO 2 /Ti 3 C 2 MXene/Ag 3 PO 4 Ternary Composite Photocatalyst toward High Photocatalytic Performance.
- Author
-
Li, Yong, Zhang, Mingqing, Liu, Yanfang, Zhao, Qinghua, Li, Xin, Zhou, Qianyu, Chen, Yuanfu, and Wang, Shifeng
- Subjects
- *
SILVER phosphates , *TITANIUM dioxide , *ELECTRON-hole recombination , *HETEROJUNCTIONS , *BRONZE , *TITANIUM composites , *ELECTRIC conductivity - Abstract
Research has demonstrated that the formation of composites of titanium dioxide (TiO2) with silver phosphate (Ag3PO4) through the construction of heterojunctions can expand its light absorption range and suppress the recombination of photogenerated electron–hole pairs, thereby improving the photocatalytic performance. However, this method offers only limited performance improvements, and the composite photocatalysts are costly due to the expensive Ag3PO4. In this study, Ti3C2 MXene, which has good hydrophilicity and excellent electrical conductivity, is first used to form Schottky junction composites with bronze TiO2 (TiO2(B)) via electrostatic self-assembly. Then, Ag3PO4 quantum dots were further formed on the surface of the TiO2(B)/Ti3C2 MXene by in situ self-growth, and Ag3PO4 formed heterojunctions and Schottky junctions with TiO2(B) and Ti3C2 MXene, respectively. Finally, a ternary composite photocatalyst TiO2(B)/Ti3C2 MXene/Ag3PO4 was jointly constructed by these functional junctions. Under the synergistic effect of these functional junctions, the mobility and fast separation performance of photogenerated electron–hole pairs of the composite photocatalyst were significantly improved, the recombination of photogenerated electron–hole pairs was effectively suppressed, and the light absorption performance was enhanced. As a result, the composite photocatalyst exhibited excellent photocatalytic performances. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
21. Colorectal Cancer Screening With High Risk-Factor Questionnaire and Fecal Immunochemical Tests Among 5, 947, 986 Asymptomatic Population: A Population-Based Study.
- Author
-
Zhang, Mingqing, Zhao, Lizhong, Zhang, Yongdan, Jing, Haoren, Wei, Lianbo, Li, Zhixuan, Zhang, Haixiang, Zhang, Yong, Zhu, Siwei, Zhang, Shiwu, and Zhang, Xipeng
- Subjects
EARLY detection of cancer ,COLORECTAL cancer ,INTESTINAL diseases ,MEDICAL screening ,ADENOMATOUS polyps ,CITIES & towns - Abstract
Background: The recent uptrend in colorectal cancer (CRC) incidence in China is causing an increasingly overwhelming social burden. And its occurrence can be effectively reduced by sensitizing CRC screening for early diagnosis and treatment. However, a large number of people in China do not undergo screening due to multiple factors. To address this issue, since 2012, a CRC screening program has been initiated in Tianjin. Methods: Residents aged 40-74 years were eligible for CRC screening. The first was to complete the high-risk factor questionnaire (HRFQ) and undergo fecal immunochemical test (FIT). Then those with a positive result in any of the two screening methods were recommended for a free colonoscopy. Results: The detection rate of intestinal diseases increased with age, had a male predominance, and was higher in residents from central urban areas and those with primary school above education level. The sensitivity of predicting CRC after colonoscopy in the high-risk group was 76.02%; the specificity was 25.33%.A significant decrease in the detection rate of intestinal disease, CRC and advanced adenoma was observed from positive FIT, the high-risk group and positive HRFQ, 47.13%, 44.79%, 42.30%; 3.15%, 2.44%, 1.76%; 7.72%, 6.42%, 5.08%, in that order, while no inter-group difference was found for the detection of polyps. In addition, the different combinations of HRFQ and FIT can enroll more high-risk population than FIT or (and) HRFQ only, and thus detect more intestinal diseases (include CRC/AA/Polyp). Conclusion: The superimposition of different screening method for HRFQ and FIT is an effective strategy for the detection of CRC, AA, and Polyp, compared to HRFQ or FIT alone. However, further improvements in screening and interventions are needed to promote colonoscopy compliance. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
22. A signature based on 11 autophagy genes for prognosis prediction of colorectal cancer.
- Author
-
Chen, Shuo, Wang, Yan, Wang, Boxue, Zhang, Lin, Su, Yinan, Xu, Mingyue, and Zhang, Mingqing
- Subjects
COLORECTAL cancer ,RECEIVER operating characteristic curves ,NOMOGRAPHY (Mathematics) ,PROGNOSIS ,TUMOR-infiltrating immune cells ,IMMUNE checkpoint proteins ,GENE expression profiling - Abstract
Aim: To develop an autophagy-gene-based signature that could help to anticipate the therapeutic effects of Colorectal Cancer (CRC). Methods: We downloaded the gene expression profiles of CRC samples from the Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) datasets. Genes with significant prognostic value in CRC were screened through univariate Cox regression analysis, while the LASSO Cox regression method was applied to screen optimal genes to construct the autophagy‐related prognostic signature. Results: 11 autophagy genes were identified and selected for the establishment of prognosis prediction model for CRC patients. The CRC patients were classified into the low- and high-risk groups according to the optimal cutoff value. The time-dependent ROC curves indicated the good performance of this model in prognosis prediction, with AUC values of 0.66, 0.66, and 0.67 at 1, 3 and 5 years for TCGA samples, as well as AUC values of 0.63, 0.65 and 0.64 for GEO samples, respectively. The multivariate Cox regression analysis results confirmed risk score as the independent marker for prognosis prediction in CRC. Besides, the constructed nomogram also had high predictive value. The results analysis on the tumor infiltrating immune cells (TIICs) relative ratios and mRNA levels of key immune checkpoint receptors indicated the signature was closely related to immune microenvironment of CRC in the context of TIICs and immune checkpoint receptors' mRNA level. The proportion of MSI-L + MSI-H in the high-risk group was higher than that in the low-risk group. Moreover, the tumor purity was evaluated by estimate function package suggested that lower tumor purity in CRC might lead to a poorer prognosis. Conclusion: The autophagy-related features obtained in this study were able to divide the CRC patients into low- and high-risk groups, which should be contribute to the decision-making of CRC treatment. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
23. The Fecal Microbiota Transplantation: A Remarkable Clinical Therapy for Slow Transit Constipation in Future.
- Author
-
Liu, Jiafei, Gu, Liqiang, Zhang, Mingqing, Zhang, Shiwu, Wang, Min, Long, Yu, and Zhang, Xipeng
- Subjects
FECAL microbiota transplantation ,CONSTIPATION ,INTESTINES ,INTESTINAL diseases ,SHORT-chain fatty acids - Abstract
Slow transit constipation is a common condition that would be difficult to treat in clinical practice with a widespread incidence in the population. Pharmacotherapy and surgery are common treatment modalities. However, the clinical effect is limited, and patients still suffer from it. As the researchers strived in this field for decades, the profound relationship between slow transit constipation and fecal microbiota transplantation has comprehensively been sustained. It is very pivotal to maintain intestinal homeostasis, the structure function and metabolic function of symbiotic bacteria, which can inhibit the engraftment of intestinal pathogens. This mini review explains the treatment effects and possible mechanisms of the fecal microbiota transplantation in treating slow transit constipation. Simultaneously, it is found that there is significant improvement in the disease by adjusting the intestinal microbes like fecal microbiota transplantation. Fecal microbiota transplantation has efficient therapeutic effects in slow transit constipation compared with traditional therapies. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
24. Effects of Clostridium butyricum on growth, digestive enzyme activity, antioxidant capacity and gut microbiota in farmed tilapia (Oreochromis niloticus).
- Author
-
Zhang, Mingqing, Dong, Bo, Lai, Xingxing, Chen, Zhuang, Hou, Liping, Shu, Rui, Huang, Yanhua, and Shu, Hu
- Subjects
- *
DIGESTIVE enzymes , *NILE tilapia , *CLOSTRIDIUM butyricum , *GUT microbiome , *OXIDANT status , *TILAPIA - Abstract
The effects of a commercial probiotic (Clostridium butyricum) on growth, gut microbiota, digestion‐related enzyme activity, antioxidant capacity and water quality in genetically improved, farmed tilapia (GIFT; Oreochromis niloticus) were assessed in a closed‐circuit, container culture system. A basal diet containing commercially available C. butyricum at 3.0 × 1010, 1.5 × 1011 and 3.0 × 1011 CFU/kg, and a control diet were fed to tilapia fries for 90 days. Growth performances and water quality were improved by probiotic treatment. Activities of digestive enzymes (amylase, lipase and trypsin) were improved, especially with 1.5 × 1011 CFU/kg (p < 0.05). After feeding for 45 days and 90 days, antioxidant capacity in the liver, spleen and head kidney, and the plasma immunity in the treatment groups improved. The analysis of 16S rRNA indicated that C. butyricum did not significantly increase its abundance in the hindgut, but could modulate gut microbial communities and significantly increase functions relating to nitrogen metabolism, phosphorylation and proteinases. These results support the administration of C. butyricum, especially at 1.5 × 1011 CFU/kg. As a probiotic for high‐density tilapia, C. butyricum improves water quality, growth performance, antioxidant capacity and the immune response of GIFT under crowding stress. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
25. Formation of Polyploid Giant Cancer Cells Involves in the Prognostic Value of Neoadjuvant Chemoradiation in Locally Advanced Rectal Cancer.
- Author
-
Fei, Fei, Zhang, Mingqing, Li, Bo, Zhao, Lizhong, Wang, Hui, Liu, Lina, Li, Yuwei, Ding, Po, Gu, Yanjun, Zhang, Xipeng, Jiang, Tao, Zhu, Siwei, and Zhang, Shiwu
- Subjects
- *
RECTAL cancer , *CANCER cells , *CANCER stem cells , *CHEMORADIOTHERAPY , *COLON cancer , *SABBATH - Abstract
We previously reported that polyploid giant cancer cells (PGCCs) exhibit cancer stem cell properties and can generate daughter cells with the epithelial-mesenchymal transition phenotype. This study investigated the role of PGCC formation in the prognostic value of neoadjuvant chemoradiation therapy (nCRT) in locally advanced rectal cancer (LARC). The morphological characteristics were observed in patients with LARC after nCRT. Colorectal cancer cell lines were treated with irradiation or chemotherapeutic drugs, and the metastasis-related proteins were detected. 304 nCRT cases and 301 paired non-nCRT cases were collected for analysis. More PGCCs and morphologic characteristics related to invasion and metastasis appeared in tumor tissue after nCRT. Irradiation or chemicals could induce the formation of PGCCs with daughter cells exhibiting strong migratory, invasive, and proliferation abilities. In patients after nCRT, pathologic complete remission, partial remission, stable disease, and progressive disease were observed in 29 (9.54%), 125 (41.12%), 138 (45.39%), and 12 (3.95%) patients, respectively. Mucinous adenocarcinomas (MCs) occurred more frequently in nCRT than in non-nCRT patients (χ2 = 29.352, P=0.001), and the prognosis in MC patients was worse than that in non-MC patients (χ2 = 24.617, P=0.001). The difference in survival time had statistical significance for 60 days (χ2 = 5.357, P=0.021) and 70 days (χ2 = 18.830, P=0.001) rest interval time. On multivariable analysis, 60 days rest interval, Duke's stage, and recurrence and/or distant metastasis remained significant predictors of survival. In conclusion, irradiation or chemicals induce the formation of PGCCs and PGCCs produce daughter cells with strong migration and invasion abilities after a long incubation period. Appropriate rest interval (incubation period) is very important for patients with LARC who will receive nCRT. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
26. The role of mSEPT9 in screening, diagnosis, and recurrence monitoring of colorectal cancer.
- Author
-
Sun, Jie, Fei, Fei, Zhang, Mingqing, Li, Yuwei, Zhang, Xipeng, Zhu, Siwei, and Zhang, Shiwu
- Subjects
COLORECTAL cancer ,FECAL occult blood tests ,ADENOMATOUS polyps ,INTESTINAL polyps ,TUMOR markers ,CARCINOEMBRYONIC antigen - Abstract
Background: The application of circulating, cell-free, methylated Septin9 (mSEPT9) DNA in screening and recurrence monitoring is highly promising. CpG island methylator phenotype (CIMP) is associated with microsatellite instability (MSI). The present study was performed to determine the diagnostic accuracy of mSEPT9 for colorectal cancer (CRC) and to evaluate its utility in CRC screening and recurrence monitoring.Methods: For screening and diagnosis of CRC, peripheral mSEPT9 detection and fecal occult blood test (FOBT) were performed in 650 subjects, then the level of CEA, CA19-9 and CA724 was quantified in 173 subjects. Clinicopathological parameters and mismatch repair protein were detected among subjects with CRC. For recurrence monitoring of CRC, the sensitivity of mSEPT9 of 70 subjects was compared with tumor markers and contrast enhanced computed tomography (CECT).Results: Seventy-three percent of CRC patients were mSEPT9-positive at 94.5% specificity, and 17.1% of patients with intestinal polyps and adenoma were mSEPT9-positive at 94.5% specificity, which were higher than FOBT for the screening of CRC. The sensitivity and specificity of mSEPT9 for diagnosis and recurrence monitoring were higher than that of CEA, CA19-9 and CA724. The combined detection of mSEPT9 and CECT enhanced the sensitivity for recurrence monitoring. Pre-therapeutic levels of mSEPT9 were strongly associated with TNM stage, Dukes stages and mismatch repair deficiency (dMMR).Conclusions: mSEPT9 analysis might be popularized as a routine biomarker for CRC screening. The combined detection of mSEPT9 and CECT can play an important role for recurrence monitoring. CIMP was highly associated with the pathological stage of CRC and dMMR. [ABSTRACT FROM AUTHOR]- Published
- 2019
- Full Text
- View/download PDF
27. Effect of fasting and subsequent refeeding on the transcriptional profiles of brain in juvenile Spinibarbus hollandi.
- Author
-
Yang, Yang, Zhou, Huiqiang, Shu, Hu, Zhong, Dongming, Zhang, Mingqing, and Xia, Jun Hong
- Subjects
PHYSIOLOGY ,COMPUTATIONAL biology ,DNA replication ,CARBOHYDRATE metabolism ,CYTOLOGY ,LIPID metabolism - Abstract
Starvation is a common stress in fish. The underlying molecular mechanisms associated with growth depression caused by feeding restriction and compensatory growth are not well understood. We investigated the effect of fasting and refeeding on the transcriptome profiles of brain in juvenile S. hollandi using RNA-seq. A total of 4.73 × 10
8 raw reads were obtained from nine brain samples. De novo transcriptome assembly identified 387,085 unigenes with 2.1×109 nucleotides. A total of 936 annotated unigenes showed significantly differential expression among the control, fasting, and fasting-refeeding groups. The down-regulated differentially expressed genes (DEGs) during fasting were mainly associated with cell cycle, DNA replication, and mitosis. The up-regulated DEGs were mainly related to glucose and lipid metabolism, material transportation, and transcription factors. Most decreased DEGs during fasting were restored to normal levels after refeeding. Comparing with the control group, genes associated with protein synthesis, stimulus response, and carbohydrate metabolism were significantly over-expressed and pro-opio melanocortin (POMC) was down-regulated during the refeeding period. In conclusion, fish mobilized stored energetic materials and reduced energy consumption to prolong survival during fasting. After refeeding, the down-regulation of DEGs, e.g., POMC may be associated with compensatory growth. Up-regulation of DEGs related to ribosomal protein, stimulus response, and carbohydrate metabolism may contribute to eliminate negative effect of starvation on brain. This study provided the first transcriptome data related with impact of short-time starvation and refeeding in S. hollandi brains. [ABSTRACT FROM AUTHOR]- Published
- 2019
- Full Text
- View/download PDF
28. Composition and distribution of bacterial communities and antibiotic resistance genes in fish of four mariculture systems.
- Author
-
Zhang, Mingqing, Hou, Liping, Zhu, Yating, Zhang, Cuiping, Li, Wen, Lai, Xingxing, Yang, Jinlin, Li, Shuisheng, and Shu, Hu
- Subjects
DRUG resistance in bacteria ,MARICULTURE ,BACTERIAL communities ,FISH communities ,WHITELEG shrimp ,GENES ,GROUPERS - Abstract
Fish-related antibiotic resistance genes (ARGs) have attracted attention for their potentially harmful effects on food safety and human health through the food chain transfer. However, the potential factors affecting these ARGs have not been fully explored. In this study, ARGs and bacterial communities in the fish gut, mucosal skin, and gill filaments in fish were comprehensively evaluated in four different mariculture systems formed by hybrid grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂), Gracilaria bailinae , and Litopenaeus vannamei using different combinations. The results showed that 9 ARGs were detected in the gut and mucosal skin and 6 ARGs in the gill filaments. The detection rate of aphA1 was the highest, and the abundance was 1.91 × 10
−3 - 6.30 × 10−2 copies per 16 S rRNA gene. Transposase gene (tnpA-04) was detected in all samples with the abundance of 3.57 × 10−3 - 3.59 × 10−2 copies per 16 S rRNA gene, and was strongly correlated with multiple ARGs (e.g., aphA1 , tet(34) , mphA-02). Proteobacteria, Deinococcus-Thermus, Firmicutes, and Bacteroidetes were the dominant phyla in the four mariculture systems, accounting for 65.1%–96.2% of the total bacterial community. Notably, the high relative abundance of Stenotrophomonas , a potential human pathogen, was elevated by 20.5% in the hybrid grouper gut in the monoculture system. In addition, variation partitioning analysis (VPA) showed that the difference in bacterial communities between mariculture systems was the main driving factor of ARGs distribution differences in hybrid groupers. This study provides a new comprehensive understanding of the characterization of fish-related ARGs contamination in different mariculture systems and facilitates the assessment of potential risks of ARGs and pathogen taxa to human health. [Display omitted] • The mucosal skin and gill in IMTA systems had higher ARGs abundance. • High abundance of the pathogen Stenotrophomonas in grouper monoculture system. • Bacterial differences between aquaculture systems drove ARGs differences. • AphA1 / tnpA-04 result suggests aminoglycoside resistance/transmission risk. • IMTA of fish-algae is a recommended production method for groupers. [ABSTRACT FROM AUTHOR]- Published
- 2022
- Full Text
- View/download PDF
29. Cloning, expression and cellular localization of the Doublesex gene in the water flea, Daphnia carinata, during different developmental stages.
- Author
-
Zhang, Mingqing, Li, Haixia, Liu, Ajing, Wu, Donglei, Wang, Danli, and Zhao, Yunlong
- Subjects
- *
MOLECULAR cloning , *GENE expression , *DOUBLESEX gene , *CLADOCERA , *INSECT development , *GENE amplification , *SEX differentiation (Embryology) - Abstract
In this study, one of Doublesex genes from the common freshwater cladoceran Daphnia carinata , designated DapcaDsx1 , was cloned using primers based on homologous sequences and rapid amplification of cDNA ends (RACE). qPCR was employed to quantify differences in DapcaDsx1 expression between the different sexual phases, with expression levels being higher in sexual females. The role of DapcaDsx1 in the reproductive transformation was further investigated in parthenogenetic-phase females and sexual-phase females using whole-mount in situ hybridization. This cellular localization study showed specific expression of DapcaDsx1 in the thoracic segments, second antenna and part of the ventral carapace. Higher expression levels were exhibited in sexual females compared to parthenogenetic females. This suggests that the DapcaDsx1 gene plays significant roles in switching modes of reproduction and during sexual differentiation. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
30. Preparation of Ag 3 PO 4 /TiO 2 (B) Heterojunction Nanobelt with Extended Light Response and Enhanced Photocatalytic Performance.
- Author
-
Li, Yong, Liu, Yanfang, Zhang, Mingqing, Zhou, Qianyu, Li, Xin, Chen, Tianlu, and Wang, Shifeng
- Subjects
HETEROJUNCTIONS ,SOLAR radiation ,ELECTRON-hole recombination ,PHOTONS ,POWER resources ,PHOTODEGRADATION ,QUANTUM efficiency - Abstract
Photocatalytic degradation, as an emerging method to control environmental pollution, is considered one of the most promising environmental purification technologies. As Tibet is a region with some of the strongest solar radiation in China and even in the world, it is extremely rich in solar energy resources, which is ideal for applying photocatalytic technology to its ecological environment protection and governance. In this study, Na
2 Ti3 O7 nanobelts were prepared via a hydrothermal method and converted to TiO2 ∙xH2 O ion exchange, which was followed by high-temperature calcination to prepare TiO2 (B) nanobelts ("B" in TiO2 (B) means "Bronze phase"). A simple in situ method was used to generate Ag3 PO4 particles on the surface of the TiO2 nanobelts to construct a Ag3 PO4 /TiO2 (B) heterojunction composite photocatalyst. By generating Ag3 PO4 nanoparticles on the surface of the TiO2 (B) nanobelts to construct heterojunctions, the light absorption range of the photocatalyst was successfully extended from UV (ultraviolet) to the visible region. Furthermore, the recombination of photogenerated electron–hole pairs in the catalyst was inhibited by the construction of the heterojunctions, thus greatly enhancing its light quantum efficiency. Therefore, the prepared Ag3 PO4 /TiO2 (B) heterojunction composite photocatalyst greatly outperformed the TiO2 (B) nanobelt in terms of photocatalytic degradation. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF
31. Differential Mucosal Microbiome Profiles across Stages of Human Colorectal Cancer.
- Author
-
Zhang, Mingqing, Lv, Yongming, Hou, Shaobin, Liu, Yanfei, Wang, Yijia, and Wan, Xuehua
- Subjects
- *
COLORECTAL cancer , *TUMOR markers , *GUT microbiome , *FUSOBACTERIUM , *DIAGNOSIS - Abstract
Emerging evidences link gut microbiota to colorectal cancer (CRC) initiation and development. However, the CRC stage- and spatial-specific bacterial taxa were less investigated, especially in a Chinese cohort, leading to our incomplete understanding of the functional roles of gut microbiota in promoting CRC progression and recurrence. Here, we report the composition and structure of gut microbiota across CRC stages I, II and III, by analyzing the gut mucosal microbiomes of 75 triplet-paired samples collected from on-tumor, adjacent-tumor and off-tumor sites and 26 healthy controls. We observed tumor-specific pattern of mucosal microbiome profiles as CRC progressed and identified ten bacterial taxa with high abundances (>1%) as potential biomarkers for tumor initiation and development. Peptostreptococcus and Parvimonas can serve as biomarkers for CRC stage I. Fusobacterium, Streptococcus, Parvimonas, Burkholderiales, Caulobacteraceae, Delftia and Oxalobacteraceae can serve as biomarkers for CRC stage II, while Fusobacterium, Burkholderiales, Caulobacteraceae, Oxalobacteraceae, Faecalibacterium and Sutterella can serve as biomarkers for CRC stage III. These biomarkers classified CRC stages I, II and III distinguished from each other with an area under the receiver-operating curve (AUC) > 0.5. Moreover, co-occurrence and co-excluding network analysis of these genera showed strong correlations in CRC stage I, which were subsequently reduced in CRC stages II and III. Our findings provide a reference index for stage-specific CRC diagnosis and suggest stage-specific roles of Peptostreptococcus, Fusobacterium, Streptococcus and Parvimonas in driving CRC progression. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
32. Cloning, expression and cellular localization of Daphnia pulex senescence-associated protein, DpSAP.
- Author
-
Liu, Ajing, Kong, Ling, Zhang, Mingqing, Wu, Donglei, Wang, Danli, and Zhao, Yunlong
- Subjects
- *
MOLECULAR cloning , *GENE expression , *DAPHNIA pulex , *PROTEIN genetics , *POLYMERASE chain reaction , *RNA probes - Abstract
Abstract: Daphnia (water fleas) are small crustaceans that undergo an unusual switch from asexual to sexual reproduction that is dependent on environmental conditions. In this study, a senescence-associated protein (SAP) from the common freshwater species Daphnia pulex was cloned using primers based on homologous sequences and rapid amplification of cDNA ends (RACE). Real-time PCR was employed to quantify the expression of D. pulex SAP (DpSAP) in individual organisms. The role of DpSAP in the reproductive transformation was further investigated in both parthenogenetic and sexual females by using digoxin-labeled SAP RNA probes and RNA whole-mount in situ hybridization. DpSAP was more highly expressed in sexual females, indicating a role in growth and reproduction. Cellular localization studies using RNA whole-mount in situ hybridization showed specific expression in the second tentacle joints. These expression patterns suggest an important role for DpSAP in the reproductive transformation of D. pulex. [Copyright &y& Elsevier]
- Published
- 2014
- Full Text
- View/download PDF
33. Case report: Successful treatment of advanced colon cancer in an eighty-year-old man with long-term and multi-stage endoscopic minimally invasive therapy.
- Author
-
Zhang N, Zhu L, Liu Y, Chen X, Zhang B, Wen C, Zhang H, Tang Q, and Zhang M
- Abstract
Background: No previous studies have reported on the use of minimally invasive endoscopic therapy for colon cancer in older patients., Case Presentation: An 80-year-old man was admitted to our hospital with haematochezia and diagnosed with advanced colon cancer in 2018. Traditional surgical care was rejected by his family. We successfully treated the patient with multiple minimally invasive endoscopic therapies, such as argon plasma coagulation, from 2018 to 2021., Conclusion: Invasive endoscopic therapy is a feasible way to treat colon cancer in older patients., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Zhang, Zhu, Liu, Chen, Zhang, Wen, Zhang, Tang and Zhang.)
- Published
- 2024
- Full Text
- View/download PDF
34. Fecal Immunochemical Testing and the Risk of Advanced Colorectal Neoplasia: A Difference-In-Difference Analysis.
- Author
-
Zhang G, Wang Y, Zhao L, Zhang M, Zhang W, Zhang W, Zhang S, Zhang H, Wang D, Wang Y, Xie L, Qian B, and Zhang X
- Subjects
- Humans, Adult, Middle Aged, Aged, Prospective Studies, Occult Blood, China epidemiology, Colonoscopy, Colorectal Neoplasms diagnosis, Colorectal Neoplasms epidemiology
- Abstract
Purpose: To evaluate the effectiveness of fecal immunochemical testing (FIT) in colorectal cancer screening., Methods: We conducted a prospective cohort study among 5,598 participants age 40-74 years between 2012 and 2020 in Tianjin, China. Inverse probability weighting was adopted to adjust for potential imbalanced factors between groups. A Cox proportional hazards model was used to estimate the weighted associations between FIT screening and advanced colorectal neoplasia. A difference-in-difference (DID) model was adopted to compare the incidence rates of advanced colorectal neoplasia between groups., Results: In DID analysis, the rate of incidence was reduced by 0.34 cases per person-years in the screening group as compared with the historical FIT screening group (rate ratio [RR], 0.08 [95% CI, 0.07 to 0.10]) and by 0.06 cases per person-years in the non-FIT screening group as compared with the historical non-FIT screening group (RR, 0.37 [95% CI, 0.29 to 0.48]; P < .001 for both comparisons), with a relative reduction of 0.28. Similar benefit effect from FIT screening was observed in sex and age subgroups., Conclusion: FIT screening was associated with a reduction in incidence density from advanced colorectal neoplasia.
- Published
- 2024
- Full Text
- View/download PDF
35. Postponing colonoscopy for 6 months in high-risk population increases colorectal cancer detection in China.
- Author
-
Zhang M, Zhang Y, Zhang W, Zhao L, Jing H, Wu X, Guo L, Zhang H, Zhang Y, Zhu S, Zhang S, and Zhang X
- Subjects
- Humans, Retrospective Studies, Colonoscopy, Risk Factors, Early Detection of Cancer methods, China epidemiology, Mass Screening methods, Colorectal Neoplasms diagnosis, Colorectal Neoplasms epidemiology, Adenoma diagnosis, Adenoma epidemiology
- Abstract
Background and Aims: Colonoscopy is an important colorectal cancer (CRC) screening modality; however, not all high-risk groups identified by fecal immunochemical test (FIT) and/or high-risk factor questionnaire (HRFQ) undergo colonoscopy in time. The impact of delays in colonoscopy on CRC detection among high-risk populations remains poorly understood, warranting further clarification., Methods: A retrospective study was conducted among CRC high-risk population identified by Tianjin CRC screening program. According to the colonoscopy results after HRFQ and FIT, patients were classified into CRC, advanced adenoma, non-advanced adenoma, and normal groups. The time interval between CRC screening and colonoscopy was investigated and its relationship with colonoscopy results. Logistic regression was performed to explore the risk factors of CRC detection., Results: Among the high-risk population without a history of CRC or polyps, 49,810 underwent HRFQ, FIT, and colonoscopy, and a time interval of fewer than 6 months was found for 79.56% of patients (n = 39,630). People with positive FIT were more likely to undergo colonoscopy within 6 months, and detection rates of CRC and/or advanced adenoma were positively related to time intervals. Similar results were found in people with a negative FIT but positive HRFQ. A time interval longer than 6 months was a significant predictor of CRC detection in high-risk populations., Conclusion: For high-risk people identified by CRC screening, especially those with a positive FIT, a time interval of 6 months was associated with an increased probability of CRC detection. Our findings emphasize that populations at high risk should undergo colonoscopy at least within 6 months., (© 2023 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.)
- Published
- 2023
- Full Text
- View/download PDF
36. Downregulated Circulating Long Non-coding RNA GAS6-AS1 Screens and Predicts Acute Myocardial Infarction.
- Author
-
Wang Z, Zhang M, and Fu Y
- Subjects
- Humans, Creatine, Creatine Kinase, Down-Regulation, RNA, Long Noncoding, Myocardial Infarction
- Abstract
Background: Acute myocardial infarction seriously threatens human health and life quality, which needs novel biomarkers to improve its early detection and development prediction. This study aimed to assess the potential of long non-coding RNA GAS6-AS1 in discriminating acute myocardial infarction patients and predicting patients' outcomes., Methods: The circulating expression of GAS6-AS1 in 83 acute myocardial infarction patients and 62 healthy individuals was evaluated using polymerase chain reaction. The value of GAS6-AS1 in the distinguishing acute myocardial infarction patients was evaluated with receiver operating characteristic analysis, and its prognosis predictive potential was assessed by Kaplan-Meier and Cox analysis. Additionally, the correlation of GAS6-AS1 with patients' critical features was evaluated by Spearman's correlation analysis., Results: Significant downregulation of GAS6-AS1 was observed in the plasma of acute myocardial infarction patients relative to healthy individuals. Reduced GAS6-AS1 could discriminate acute myocardial infarction patients from healthy controls and indicate patients' unoptimistic prognosis. Moreover, GAS6-AS1 was found to be negatively cor-related with the levels of creatine kinase, creatine kinasemyocardial bland, lactic dehy-drogenase, hydroxybutyrate dehydrogenase, troponin T, and positively correlated with the ejection fraction of acute myocardial infarction patients., Conclusion: Changes in circulating GAS6-AS1 in acute myocardial infarction served as a potential diagnostic and prognostic biomarker of acute myocardial infarction.
- Published
- 2023
- Full Text
- View/download PDF
37. RNA-seq and qRT-PCR analyses reveal the physiological response to acute hypoxia and reoxygenation in Epinephelus coioides .
- Author
-
Lai X, Zhong Z, Lin B, Wu Y, Ma Y, Zhang C, Yang Y, Zhang M, Qin W, Fu X, and Shu H
- Abstract
Hypoxia is a critical problem in intensive Epinephelus coioides aquaculture systems. In the present study, the physiological responses of E. coioides muscle to acute hypoxic stress (DO = 0.6 ± 0.1 mg/L) and reoxygenation (DO = 6.0 ± 0.1 mg/L) were analyzed by transcriptome sequencing (RNA-seq) and quantitative real-time PCR (qRT-PCR). RNA-seq was conducted on the muscle tissues of E. coioides in the hypoxia-tolerant (EMS), hypoxia-sensitive (EMW), and normoxic (CM) groups. Among the three groups, a total of 277 differentially expressed genes (DEGs) were identified. KEGG analysis revealed that the pathways significantly enriched after hypoxic stress are involved in the immune response, glycolysis/gluconeogenesis, energy metabolism, vasodilation and proliferation, cell proliferation, and apoptosis. qRT‒PCR verified that the differentially expressed genes FIH-1, PHD-2, PPARα , BCL-XL , LDH-A , and Flt-1 were significantly upregulated after hypoxic stress and returned to normal levels after reoxygenation, suggesting that these DEGs play important roles in responding to hypoxia treatment. In addition, the HIF-1 signaling pathway was also activated under hypoxic stress, and qRT‒PCR confirmed that the expression level of HIF-1α was significantly elevated under acute hypoxic stress, indicating that the HIF-1 signaling pathway is the central pathway in the E. coioides hypoxic response mechanism and activates other related pathways to adapt to hypoxic stress. These pathways jointly regulate energy metabolism, substance synthesis, blood vessel proliferation, cell proliferation, and differentiation and prolong survival time. These results provide ideas for understanding physiological regulation after hypoxic stress and reoxygenation and provide basic insights for the future breeding of hypoxia-tolerant E. coioides ., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Lai, Zhong, Lin, Wu, Ma, Zhang, Yang, Zhang, Qin, Fu and Shu.)
- Published
- 2022
- Full Text
- View/download PDF
38. Predictive models based on machine learning for bone metastasis in patients with diagnosed colorectal cancer.
- Author
-
Li T, Huang H, Zhang S, Zhang Y, Jing H, Sun T, Zhang X, Lu L, and Zhang M
- Subjects
- Algorithms, Area Under Curve, Humans, Machine Learning, Artificial Intelligence, Colorectal Neoplasms diagnosis
- Abstract
Background: This study aimed to develop an artificial intelligence predictive model for predicting the probability of developing BM in CRC patients., Methods: From SEER database, 50,566 CRC patients were identified between January 2015 and December 2019 without missing data. SVM and LR models were trained and tested on the dataset. Accuracy, area under the curve (AUC), and IDI were used to evaluate and compare the models., Results: For bone metastases in the entire cohort, SVM model with poly as kernel function presents the best performance, whose accuracy is 0.908, recall is 0.838, and AUC is 0.926, outperforming LR model. The top three most important factors affecting the model's prediction of BM include extraosseous metastases (EM), CEA, and size., Conclusion: Our study developed an SVM model with poly as kernel function for predicting BM in CRC patients. SVM model could improve personalized clinical decision-making, help rationalize the bone metastasis screening process, and reduce the burden on healthcare systems and patients., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Li, Huang, Zhang, Zhang, Jing, Sun, Zhang, Lu and Zhang.)
- Published
- 2022
- Full Text
- View/download PDF
39. PLK4 is a key molecule in the formation of PGCCs and promotes invasion and migration of progeny cells derived from PGCCs.
- Author
-
Fu F, Chen L, Yang X, Fan L, Zhang M, Chen S, Zheng M, Gao M, and Zhang S
- Abstract
Purpose: Cancer stem cells (CSCs) are the evil source of tumor metastasis and recurrence. Polyploid giant cancer cells (PGCCs) that exhibit the characteristics of CSCs produced daughter cells via asymmetric division. The molecular mechanisms of daughter cells derived from PGCCs with high migration, invasion, and proliferation abilities in colorectal cancer (CRC) are explored in this paper based on the bioinformatics analysis. Materials and Methods: We characterized the expression of CSC-related genes in CRCs by analyzing the mRNAsi of The Cancer Genome Atlas and survival time. Weighted gene co-expression network analysis was performed to identify the modules of the hub and key genes. The migration, invasion, and proliferation abilities of cells, the expression of epithelial-mesenchymal transition (EMT)-related proteins and polo-like kinase 4 (PLK4) were compared in LoVo and Hct116 cells with and without bufalin treatment. In addition, the expression and subcellular location of cell division cycle 25C (CDC25C) in cells before and after PLK4 knockdown were assessed. Results: Eight hub genes were screened out and positively association with mRNAsi in CRCs based on bioinformatic analysis. Among them, checkpoint Kinase-1 ( CHEK1 ), budding uninhibited by benzimidazoles 1 Homolog Beta ( BUB1B ) and PLK4 were closely associated with the prognosis of CRC patients. Bufalin could induce the formation of PGCCs in LoVo and Hct116 cell lines. PLK4 was overexpressed in PGCCs with progeny cells and progeny cells derived from PGCCs had strong migration and invasion abilities by expressing epithelial-mesenchymal transition (EMT)-related proteins. PLK4 could interact with CDC25C and promote CDC25C phosphorylation which was associated with the formation of PGCCs. Decreasing CDC25C expression in both LoVo and Hct116 PGCCs with progeny cells, while levels of pCDC25C-ser216 and pCDC25C-ser198 were increased in LoVo and decreased in Hct116 PGCCs with progeny cells. pCDC25C-ser216 located in the cytoplasm and pCDC25C-ser198 located in the nucleus in cells after bufalin treatment. Furthermore, expression of CDC25C, pCDC25C-ser216, and pCDC25C-ser198 was downregulated after PLK4 knockdown. Furthermore, the expression level of PLK4 was associated with differentiated degree, and lymph node metastasis in human CRC tissues. Conclusion: PLK4 contributes to the formation of PGCCs by regulating the expression of CDC25C and is associated with the expression and subcellular location of CDC25C, pCDC25C-ser216 and pCDC25C-ser198., Competing Interests: Competing Interests: The authors have declared that no competing interest exists., (© The author(s).)
- Published
- 2022
- Full Text
- View/download PDF
40. Bladder epithelial cell phosphate transporter inhibition protects mice against uropathogenic Escherichia coli infection.
- Author
-
Pang Y, Cheng Z, Zhang S, Li S, Li X, Li X, Zhang X, Li X, Feng Y, Cui H, Chen Z, Liu L, Li Q, Huang J, Zhang M, Zhu S, Wang L, and Feng L
- Subjects
- Animals, Epithelial Cells metabolism, Mice, Phosphate Transport Proteins metabolism, Phosphates metabolism, Phospholipases metabolism, Urinary Bladder, Escherichia coli Infections microbiology, Uropathogenic Escherichia coli metabolism
- Abstract
Urinary tract infections are predominantly caused by uropathogenic Escherichia coli (UPEC). UPEC infects bladder epithelial cells (BECs) via fusiform vesicles, escapes into the cytosol to evade exocytosis, and establishes intracellular bacterial communities (IBCs) for the next round of infection. The UPEC vesicle escape mechanism remains unclear. Here we show that UPEC senses host immune responses and initiates escape by upregulating a key phospholipase. The UPEC phospholipase PldA disrupts the vesicle membrane, and pldA expression is activated by phosphate reduction in vesicles. The host phosphate transporter PIT1 is located on the fusiform vesicle membrane, transporting phosphate into the cytosol. UPEC infection upregulates PIT1 via nuclear factor κB (NF-κB), resulting in phosphate reduction. Silencing PIT1 blocks UPEC vesicle escape in BECs, inhibits IBC formation in mouse bladders, and protects mice from UPEC infection. Our results shed light on pathogenic bacteria responding to intracellular phosphate shortage and tackling host defense and provide insights for development of new therapeutic agents to treat UPEC infection., Competing Interests: Declaration of interests The authors declare no competing interests., (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
41. [Corrigendum] Long Non-Coding RNA SOX2 Overlapping Transcript Aggravates H9c2 Cell Injury via the miR-215-5p/ZEB2 Axis and Promotes Ischemic Heart Failure in a Rat Model.
- Author
-
Tu J, Ma L, Zhang M, and Zhang J
- Published
- 2022
- Full Text
- View/download PDF
42. Effects of early hemodynamics, oxygen metabolism, and lactate dynamics on prognosis of post-cardiac arrest syndrome.
- Author
-
Zhang M, Zhang Q, Yu Y, An L, Qi Z, and Li C
- Subjects
- Hemodynamics, Humans, Lactic Acid, Oxygen, Prognosis, Cardiopulmonary Resuscitation, Hypothermia, Induced, Out-of-Hospital Cardiac Arrest therapy, Post-Cardiac Arrest Syndrome
- Published
- 2021
- Full Text
- View/download PDF
43. Long Non-Coding RNA SOX2 Overlapping Transcript Aggravates H9c2 Cell Injury via the miR-215-5p/ZEB2 Axis and Promotes Ischemic Heart Failure in a Rat Model.
- Author
-
Tu J, Ma L, Zhang M, and Zhang J
- Subjects
- Animals, Apoptosis genetics, Cell Survival genetics, Rats, Heart Failure genetics, MicroRNAs genetics, RNA, Long Noncoding genetics
- Abstract
Heart failure is a common cardiovascular disease, which has been regarded as one of the highest health care costs with high morbidity and mortality in the western countries. Long noncoding RNAs have been widely reported to regulate the initiation or progression of cardiovascular diseases. However, the specific role of SOX2 overlapping transcript (SOX2-OT) in ischemic heart failure remains uncharacterized. The present study aimed to explore the function and mechanism of SOX2-OT in ischemic heart failure. The starBase website was used to predict potential miRNAs or target mRNAs. Western blot assay was implemented to test collagen protein levels. Functional assays were conducted to evaluate the effects of SOX2-OT on H9c2 cell viability and apoptosis. RNA pull down and luciferase reporter assays were used to confirm the combination between miR-215-5p and SOX2-OT. We found out that SOX2-OT level was increased by oxygen glucose deprivation/reoxygenation treatment in H9c2 cells. Silencing of SOX2-OT ameliorated cell injury by promoting cell viability, inhibiting cell apoptosis and reducing productions of collagens. Mechanistically, miR-215-5p was confirmed to bind with SOX2-OT after prediction and screening. In addition, we discovered that miR-215-5p negatively regulated zinc finger E-box binding homeobox 2 (ZEB2) protein level by directly binding with ZEB2 3' untranslated region. Finally, we verified that SOX2-OT aggravated cell injury by targeting ZEB2 in H9c2 cells. In conclusion, SOX2-OT aggravated heart failure in vivo and promoted H9c2 cell injury via the miR-215-5p/ZEB2 axis in vitro, implying a novel insight into heart failure treatment.
- Published
- 2021
- Full Text
- View/download PDF
44. The role of m SEPT9 in screening, diagnosis, and recurrence monitoring of colorectal cancer.
- Author
-
Sun J, Fei F, Zhang M, Li Y, Zhang X, Zhu S, and Zhang S
- Subjects
- Adult, Aged, Cell-Free Nucleic Acids genetics, Colorectal Neoplasms genetics, Colorectal Neoplasms pathology, Early Detection of Cancer, Epigenesis, Genetic, Female, Humans, Male, Middle Aged, Neoplasm Recurrence, Local genetics, Neoplasm Recurrence, Local pathology, Neoplasm Staging, Sensitivity and Specificity, Septins blood, Tomography Scanners, X-Ray Computed, Colorectal Neoplasms diagnosis, DNA Methylation, Neoplasm Recurrence, Local diagnosis, Septins genetics
- Abstract
Background: The application of circulating, cell-free, methylated Septin9 (
m SEPT9) DNA in screening and recurrence monitoring is highly promising. CpG island methylator phenotype (CIMP) is associated with microsatellite instability (MSI). The present study was performed to determine the diagnostic accuracy ofm SEPT9 for colorectal cancer (CRC) and to evaluate its utility in CRC screening and recurrence monitoring., Methods: For screening and diagnosis of CRC, peripheralm SEPT9 detection and fecal occult blood test (FOBT) were performed in 650 subjects, then the level of CEA, CA19-9 and CA724 was quantified in 173 subjects. Clinicopathological parameters and mismatch repair protein were detected among subjects with CRC. For recurrence monitoring of CRC, the sensitivity ofm SEPT9 of 70 subjects was compared with tumor markers and contrast enhanced computed tomography (CECT)., Results: Seventy-three percent of CRC patients werem SEPT9-positive at 94.5% specificity, and 17.1% of patients with intestinal polyps and adenoma werem SEPT9-positive at 94.5% specificity, which were higher than FOBT for the screening of CRC. The sensitivity and specificity ofm SEPT9 for diagnosis and recurrence monitoring were higher than that of CEA, CA19-9 and CA724. The combined detection ofm SEPT9 and CECT enhanced the sensitivity for recurrence monitoring. Pre-therapeutic levels ofm SEPT9 were strongly associated with TNM stage, Dukes stages and mismatch repair deficiency (dMMR)., Conclusions:m SEPT9 analysis might be popularized as a routine biomarker for CRC screening. The combined detection ofm SEPT9 and CECT can play an important role for recurrence monitoring. CIMP was highly associated with the pathological stage of CRC and dMMR.- Published
- 2019
- Full Text
- View/download PDF
45. Unary Non-Structural Fertilizer Response Model for Rice Crops and Its Field Experimental Verification.
- Author
-
Zhang M, Li J, Chen F, and Kong Q
- Subjects
- Crop Production methods, Crops, Agricultural metabolism, Models, Theoretical, Nitrogen analysis, Soil chemistry, Agriculture methods, Fertilizers analysis, Oryza metabolism
- Abstract
The quadratic polynomial fertilizer response model (QPFM) is the primary method for implementing quantitative fertilization in crop production, but the success rate of this model's recommended fertilization rates in China is low because the model contains a high setting bias. This paper discusses a new modelling method for expanding the applicability of QPFM. The results of field experiments with 8 levels of N, P, or K fertilization showed that the dynamic trend between rice yield increases and fertilizer application rate exhibited a typical exponential relationship. Therefore, we propose a unary non-structural fertilizer response model (NSFM). The responses of 18 rice field experiments to N, P, or K fertilization indicated that the new models could significantly predict rice yields, while two experimental fitting results using the unary QPFM did not pass statistical significance tests. The residual standard deviations of 13 new models were significantly lower than that of the unary QPFM. The linear correlation coefficient of the recommended application rates between the new model and the unary QPFM reached a significant level. Theoretical analysis showed that the unary QPFM was a simplified version of the new model, and it had a higher fitting precision and better applicability.
- Published
- 2018
- Full Text
- View/download PDF
46. Exploration of the mechanism of colorectal cancer metastasis using microarray analysis.
- Author
-
Chen S, Wang Y, Zhang L, Su Y, Zhang M, Wang J, and Zhang X
- Abstract
The aim of the present study was to investigate the mechanism of metastasis in colorectal cancer (CRC) using microRNA (miRNA) and mRNA expression profiles. The mRNA and miRNA expression profiles of the GSE2509 and GSE56350 datasets were obtained from the Gene Expression Omnibus database. The differentially expressed genes (DEGs) and differentially expressed miRNAs (DEMs) were identified using the limma software package. The Database for Annotation, Visualization and Integrated Discovery was used to perform Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the DEGs. The predicted target genes associated with the DEMs were identified using the miRWalk database and the enrichment analysis was conducted using the clusterProfiler package. The miRNA-gene molecular interaction network was visualized using the Cytoscape software platform. A total of 544 DEGs and 42 DEMs were identified. DEGs were annotated in 320 GO terms and 11 KEGG pathways. Overall, 366 miRNA-gene pairs were identified and the miRNA-gene network was visualized. Furthermore, the predicted target genes were mainly classified in 12 pathways. The results of the present study suggest that fibronectin type III domain-containing 3B, cysteine rich transmembrane BMP regulator 1 and forkhead box J2 may be potential therapeutic and prognostic targets of metastatic CRC. In addition, pathways in cancer, the Wnt signaling pathway and extracellular matrix-receptor interaction may play a critical role in CRC metastasis.
- Published
- 2017
- Full Text
- View/download PDF
47. Downregulated miRNA-1269a variant (rs73239138) decreases the susceptibility to gastric cancer via targeting ZNF70.
- Author
-
Li W, Zhang H, Min P, Zhu J, Xu D, Jiang W, Ma Y, Qiu J, Xu W, Chen J, Zhang M, Li M, Yang D, Shi J, Zhang J, and Liu J
- Abstract
Although emerging evidence has indicated that single nucleotide polymorphisms (SNPs) in microRNAs (miRNAs) are associated with susceptibility to gastric cancer, a limited number of studies have revealed the underlying molecular mechanisms. In the present study, the results suggested that miR-1269a rs73239138 has a role in decreasing the risk of gastric cancer. The level of miR-1269a variant expression was significantly downregulated compared with the wild-type miR-1269a in the gastric cells (Fig. 1). Furthermore, overexpression of miR-1269a inhibited apoptosis of gastric cancer cells. Expression of the miR-1269a variant inhibited the function of miR-1269a by increasing the apoptotic rate and the expression of Bik, Bim and Bak was upregulated consistently. In addition, zinc-finger protein 70 (ZNF70) was identified to be a target gene of miR-1269a, which was downregulated by miR-1269a and upregulated by miR-1269a variant. ZNF70 was indicated to exert a role as a tumor suppressor in gastric cancer. To the best our knowledge, the present study for the first time highlights a critical role of miR-1269a variant rs73239138 in decreasing the susceptibility to gastric cancer by downregulating its expression and targeting ZNF70, which promotes apoptosis of gastric cancer cells. This SNP is indicated to serve as a potential biomarker and therapeutic target for gastric cancer.
- Published
- 2017
- Full Text
- View/download PDF
48. S100A4 in cancer progression and metastasis: A systematic review.
- Author
-
Fei F, Qu J, Zhang M, Li Y, and Zhang S
- Abstract
Metastasis is the leading cause of cancer-related death and directly associates with cancer progression, resistance to anticancer therapy, and poor patient survival. Current efforts focusing on the underlying molecular mechanisms of cancer metastasis attract a special attention to cancer researchers. The epithelial-mesenchymal transition is a complex of molecular program during embryogenesis, inflammation, tissue fibrosis, and cancer progression and metastasis. S100A4, an important member of S100 family proteins, functions to increase the tumor progression and metastasis. The molecular mechanisms of S100A4 involving in the progression and metastasis are diverse in various malignant tumors. Detection of S100A4 expression becomes a promising candidate biomarker in cancer early diagnosis and prediction of cancer metastasis and therefore, S100A4 may be a therapeutic target. This review summarized up to date advancement on the role of S100A4 in human cancer development, progression, and metastasis and the underlying molecular events and then strategies to target S100A4 expression experimentally., Competing Interests: CONFLICTS OF INTEREST No potential conflicts of interest were disclosed.
- Published
- 2017
- Full Text
- View/download PDF
49. Association of microRNA-933 variant with the susceptibility to gastric cancer.
- Author
-
Zhang Y, Ma Y, Xu W, Li W, Min P, Qiu J, Li M, Tang F, Zhang M, Yang D, and Zhang J
- Subjects
- Asian People genetics, Carcinoembryonic Antigen genetics, Case-Control Studies, Female, Genotype, Humans, Male, Middle Aged, Prognosis, Risk Factors, alpha-Fetoproteins genetics, Genetic Predisposition to Disease genetics, MicroRNAs genetics, Polymorphism, Single Nucleotide genetics, Stomach Neoplasms genetics
- Abstract
Purpose: Common single-nucleotide polymorphisms (SNPs) in microRNAs (miRs) have been shown to be associated with susceptibility to several types of human cancer. However, the association of miR-933 rs79402775 with gastric cancer (GC) has not been explored., Methods: The association between rs79402775 in miR- 933 and the risk of GC was explored in Chinese population based on MassARRAY technology. A total 374 GC patients and 999 cancer-free controls were enrolled in this study., Results: Compared with the wild-type GG, GA genotype was associated with a significantly decreased risk of GC (OR=0.542, 95% CI=0.299-0.983, p=0.044) in female subjects. Moreover, the variant was also associated with the expression of alpha fetoprotein (AFP) and carcinoembryonic antigen (CEA)., Conclusions: miR-933(rs79402775) may contribute to decreased susceptibility to GC and this SNP could be developed as a biomarker for GC prognosis.
- Published
- 2017
50. Daughter Cells and Erythroid Cells Budding from PGCCs and Their Clinicopathological Significances in Colorectal Cancer.
- Author
-
Zhang D, Yang X, Yang Z, Fei F, Li S, Qu J, Zhang M, Li Y, Zhang X, and Zhang S
- Abstract
Purpose: We previously reported that polyploid giant cancer cells (PGCCs) induced by cobalt chloride (CoCl
2 ) exhibit cancer stem cell properties. Daughter cells generated by PGCCs possess epithelial mesenchymal transition (EMT) phenotype changes and EMT plays an important role in cancer development and progression. This study investigated the characteristics of PGCCs from LoVo and HCT116 induced by CoCl2 and the clinicopathological significances of PGCCs in colorectal cancer (CRC). Materials and Methods: Western blotting and immunocytochemical staining were used to compare the expression levels of EMT-related proteins between PGCCs with budding daughter cells and the control cells. In addition, tissue samples were collected from 159 patients with CRC for analysis of PGCCs, vasculogenic mimicry (VM), and single stromal PGCCs with budding, as well as immunohistochemical staining for cathepsin B, vimentin, and hemoglobin A. Results: Single PGCCs induced by CoCl2 formed spheroids in vitro . Poorly differentiated CRCs showed the highest numbers of PGCCs and VM, and expression of cathepsin B. There was greater expression of EMT-related proteins in PGCCs with budding daughter cells than in control cells. The expression of vimentin located in PGCC nuclei. Single stomal PGCCs with budding were detected in 27.45% of well differentiated, 50% of moderately differentiated, and 90.20% of poorly differentiated CRC samples. PGCCs can generate erythroid cells that express delta-hemoglobin to form VM. Erythroid cells generated by PGCCs were positive for hemoglobin A immunocytochemical staining. Conclusion: PGCCs from LoVo and HCT116 treated by CoCl2 exhibited cancer stem cell properties. The number of PGCCs and VM were associated with CRC differentiation and daughter cells budded from PGCCs may promote the lymph node metastasis via expression of EMT-related proteins. PGCCs and their newly generated erythroid cells form VM structures., Competing Interests: Conflicts of Interest: The authors declare no conflict of interest.- Published
- 2017
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.