1. The effect of miR-10b on growth hormone in pituitary cells of Yanbian yellow cattle by somatostatin receptor 2.
- Author
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Ji J, Jin T, Lou A, Zhang R, Chen Y, Xiang S, Cui C, Yu L, and Guan L
- Subjects
- Animals, Cells, Cultured, MicroRNAs metabolism, Cattle genetics, Cattle metabolism, Gene Expression genetics, Gene Expression Regulation genetics, Growth Hormone genetics, Growth Hormone metabolism, MicroRNAs genetics, MicroRNAs physiology, Pituitary Gland cytology, Pituitary Gland metabolism, RNA, Messenger genetics, RNA, Messenger metabolism, Receptors, Somatostatin metabolism, Transfection
- Abstract
This study aimed to evaluate the effect of miR-10b on growth hormone (GH) in pituitary cells of Yanbian yellow cattle. According to analysis of GH and somatostatin receptor 2 (SSTR2) mRNA and protein expression levels, we found that miR-10b targeted 3'UTR of SSTR2. Compared with the negative control (NC) group, GH mRNA transcription and protein expression in pituitary cells of Yanbian yellow cattle were significantly increased by adding miR-10b mimics (p < .01), while these were significantly decreased by adding miR-10b inhibitor (p < .05); compared with the NC group, SSTR2 mRNA transcription and protein expression were significantly inhibited by the addition of miR-10b mimics (p < .01), while these were significantly increased by the addition of miR-10b inhibitor compared with the iNC group (p < .05). This study suggested that miR-10b could regulate GH level by regulating SSTR2 gene expression in pituitary cells of Yanbian yellow cattle., (© 2020 Japanese Society of Animal Science.)
- Published
- 2020
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