14 results on '"LI Fu-gui"'
Search Results
2. Gill remodeling in response to hypoxia and temperature occurs in the hypoxia sensitive blunt snout bream (Megalobrama amblycephala)
- Author
-
Wu, Cheng-Bin, Liu, Zi-Yin, Li, Fu-Gui, Chen, Jie, Jiang, Xia-Yun, and Zou, Shu-Ming
- Published
- 2017
- Full Text
- View/download PDF
3. Construction of tissue‐engineered lymphatic vessel using human adipose derived stem cells differentiated lymphatic endothelial like cells and decellularized arterial scaffold: A preliminary study.
- Author
-
Yang, Yi, Yang, Jian‐tao, Chen, Xiao‐hu, Qin, Ben‐gang, Li, Fu‐gui, Chen, Yun‐xian, Gu, Li‐qiang, Zhu, Jia‐kai, and Li, Ping
- Subjects
STEM cell transplantation ,ADIPOSE tissue transplantation ,ENDOTHELIAL cells ,TISSUE engineering ,LYMPHOID tissue ,TRANSPLANTATION of organs, tissues, etc. - Abstract
Abstract: We have previously demonstrated that human adipose‐derived stem cells (hADSCs) can be differentiated into lymphatic endothelial like cells. The purpose of this study was to investigate the feasibility of utilizing the induced lymphatic endothelial like cells and decellularized arterial scaffold to construct the tissue‐engineered lymphatic vessel. The hADSCs were isolated from adipose tissue in healthy adults and were characterized the multilineage differentiation potential. Decellularized arterial scaffold was prepared using the Triton x‐100 method. ADSCs were differentiated into lymphatic‐like endothelial cells, and the induced cells were then seeded onto the decellularized arterial scaffold to engineer the lymphatic vessel. The histological analyses were performed to examine the endothelialized construct. The decellularized arterial scaffold was successfully obtained and was able to maintain its vessel morphology. The isolated ADSCs can be differentiated into osteocytes and adipocytes. After seeding onto the scaffold, the seeded cells attached and grew well on the decellularized arterial scaffold. Our preliminary results demonstrated that the induced lymphatic endothelial like cells combined with decellularized arterial scaffold could be utilized to successfully engineer the lymphatic vessel. Our findings may be helpful for the development of tissue‐engineering of the lymphatic graft. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
4. Functional conservation and divergence of duplicated fibroblast growth factor receptor 1 (fgfr1) genes in blunt snout bream (Megalobrama amblycephala).
- Author
-
Zhang, Qian-Qian, Li, Fu-Gui, Qin, Bo, Chen, Jie, Jiang, Xia-Yun, and Zou, Shu-Ming
- Subjects
- *
FIBROBLAST growth factor receptors , *CYPRINIDAE , *CELL migration , *CELL proliferation , *CELL differentiation , *FISH embryology , *MESSENGER RNA , *FISHES - Abstract
Fgfr1 is a fibroblast growth factor receptor involved in regulating cell growth, proliferation, differentiation and migration. Here, we report the isolation and characterization of duplicated fgfr1 genes in blunt snout bream ( Megalobrama amblycephala ). Blunt snout bream fgfr1a and - 1b cDNAs were found to share a relatively high sequence identity of 82%. During embryogenesis, both fgfr1a and - 1b mRNAs were highly detected at zygotes but gradually decreased and then constantly expressed after 16 hpf, besides a strong expression for the fgfr1b mRNA at 12 hpf. Whole-mount in situ hybridization demonstrated that fgfr1a mRNA was transcribed at the eyes, mid-hindbrain boundary (MHB), brain, posterior somites and tailbud at 16 hpf, while the fgfr1b mRNA was only detected at the eyes and posterior somites at the same period. At 28 hpf embryos, both fgfr1a and - 1b mRNAs were expressed in the eyes, brain, pharyngeal arches and tailbud, and in the eyes, brain, pharyngeal arches and notochord at 55 hpf. In adult fish, fgfr1a mRNA was strongly expressed in the gill, gonad, brain and midgut, but examined relatively low in the skin and kidney. In contrast, the fgfr1b mRNA was highly detected in the brain and liver and quite low in the skin, gill and kidney. During starvation, both fgfr1a and - 1b mRNAs were significantly up-regulated in the intestine and liver, but down-regulated in the brain. Moreover, duplicated fgfr1 mRNAs were differentially inhibited in tissues with exogenous recombinant hGH. Our results suggest that two fgfr1 genes play important roles in regulating growth and development in blunt snout bream. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
5. Transcriptome Analysis of Blunt Snout Bream (Megalobrama amblycephala) Reveals Putative Differential Expression Genes Related to Growth and Hypoxia.
- Author
-
Li, Fu-Gui, Chen, Jie, Jiang, Xia-Yun, and Zou, Shu-Ming
- Subjects
- *
TRANSCRIPTION factors , *GENE expression , *HYPOXEMIA , *NUCLEOTIDE sequencing , *AQUACULTURE , *ANTISENSE DNA - Abstract
The blunt snout bream (Megalobrama amblycephala) is an important freshwater aquaculture species, but it is sensitive to hypoxia. No transcriptome data related to growth and hypoxia response are available for this species. In this study, we performed de novo transcriptome sequencing for the liver and gills of the fast-growth family and slow-growth family derived from ‘Pujiang No.1’ F10 blunt snout bream that were under hypoxic stress and normoxia, respectively. The fish were divided into the following 4 groups: fast-growth family under hypoxic stress, FH; slow-growth family under hypoxic stress, SH; fast-growth family under normoxia, FN; and slow-growth family under normoxia, SN. A total of 185 million high-quality reads were obtained from the normalized cDNA of the pooled samples, which were assembled into 465,582 contigs and 237,172 transcripts. A total of 31,338 transcripts from the same locus (unigenes) were annotated and assigned to 104 functional groups, and 23,103 unigenes were classified into seven main categories, including 45 secondary KEGG pathways. A total of 22,255 (71%) known putative unigenes were found to be shared across the genomes of five model fish species and mammals, and a substantial number (9.4%) of potentially novel genes were identified. When 6,639 unigenes were used in the analysis of differential expression (DE) genes, the number of putative DE genes related to growth pathways in FH, SH, SN and FN was 159, 118, 92 and 65 in both the liver and gills, respectively, and the number of DE genes related to hypoxic response was 57, 33, 23 and 21 in FH, FN, SH and SN, respectively. Our results suggest that growth performance of the fast-growth family should be due to complex mutual gene regulatory mechanisms of these putative DE genes between growth and hypoxia. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
6. Two follistatin-like 1 homologs are differentially expressed in adult tissues and during embryogenesis in grass carp (Ctenopharyngodon idellus).
- Author
-
Sun, Yi-Wen, Li, Fu-Gui, Chen, Jie, Jiang, Xia-Yun, and Zou, Shu-Ming
- Subjects
- *
FOLLISTATIN , *FISH embryology , *GENE expression in fishes , *HOMOLOGY (Biochemistry) , *CTENOPHARYNGODON idella , *MYOBLASTS - Abstract
Follistatin-like 1 (Fstl1) peptides play important roles in inhibiting myoblast proliferation and differentiation. Here, we characterized and examined the expression patterns of fstl1a and -b in grass carp ( Ctenopharyngodon idellus ). These genes encode 314 aa and 310 aa peptides, respectively, sharing a sequence identity of 83%. Except for the existence of the follistatin-N-terminal (FOLN) and Kazal-type 2 serine protease inhibitor (Kazal 2) domains, grass carp Fstl1a and -b do not share amino acid sequence similarity with Fst1 and -b. Both fstl1a and -b mRNAs were widely expressed in adult tissues. During embryogenesis, grass carp fstl1a and -b mRNA was detected in the presomitic mesoderm and somites at 12 h post fertilization (hpf). At 24 hpf, fstl1a mRNA was expressed in the hindbrain, somites, notochord and tailbud, while fstl1b mRNA was only detected in the tailbud. At 36 hpf, fstl1a mRNA was detected in the hindbrain and notochord, and fstl1b was also expressed in the notochord. Furthermore, fstl1a and -b were downregulated in brain and liver tissue following injection with 10 or 50 μg hGH, while fstl1b was significantly up-regulated in muscle tissue after 10 μg hGH treatment. Both fstl1a and -b were significantly up-regulated at 2, 4 or 6 days of nutrient restriction, and fstl1a was still highly expressed in the liver and muscle after 3 days of refeeding, as was fstl1b in the brain and muscle. The expression of these genes returned to near control levels following 6 days of refeeding. Our findings suggest that the two fstl s play important but divergent roles in embryonic development and tissue growth regulation in grass carp. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
7. Duplicated connective tissue growth factor genes in hypoxia-sensitive blunt snout bream Megalobrama amblycephala and their in vivo expression.
- Author
-
Wang, Yao, Li, Fu-Gui, Qin, Bo, Chen, Jie, Jiang, Xia-Yun, and Zou, Shu-Ming
- Subjects
- *
CONNECTIVE tissue growth factor , *HYPOXEMIA , *GENE expression in fishes , *CYPRINIDAE , *FISH development , *MESSENGER RNA - Abstract
Connective tissue growth factor (CTGF) is a peptide involved in tissue growth and development, and can be regulated by hypoxia stress. This study aimed to isolate and characterize duplicate Ctgf genes in blunt snout bream Megalobrama amblycephala , and determine their expression patterns and response to hypoxia. The blunt snout bream Ctgfa and Ctgfb were found to be highly divergent, sharing a relatively low sequence identity of 57%. During embryogenesis, Ctgfa mRNA expression levels were low, gradually decreased from zygotes to 12 h post-fertilization (hpf), markedly increased from 16 hpf, and then stabilized from 32 to 40 hpf. Ctgfb expression levels were constant but low from zygotes to 20 hpf, then gradually increased from 24 to 40 hpf. Ctgfa mRNA was expressed in the adaxial cells of the somites, floor plate, and tailbud at 24 hpf, and in the notochord and ethmoid plate at 36 hpf, whereas Ctgfb mRNA was weakly expressed in the adaxial cells and floor plate at 24 hpf, and in the notochord at 36 hpf. In adult fish, Ctgfa mRNA was strongly expressed in the kidney, brain, intestine, muscles, and skin, while Ctgfb mRNA was detected in all examined tissues. During hypoxic treatment, the mRNA levels of both Ctgfa and - b were significantly upregulated in the gill and liver, whereas Ctgfa mRNAs in the brain and kidney and Ctgfb mRNAs in the kidney significantly decreased. These results provide new insights into the functional conservation and divergence of Ctgf genes and reveal their responses to hypoxia. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
8. In Vitro Induction of Human Adipose-Derived Stem Cells into Lymphatic Endothelial-Like Cells.
- Author
-
Yang, Yi, Chen, Xiao-hu, Li, Fu-gui, Chen, Yun-xian, Gu, Li-qiang, Zhu, Jia-kai, and Li, Ping
- Subjects
LYMPHEDEMA treatment ,HUMAN stem cells ,VASCULAR endothelial growth factor receptors ,IMMUNOSTAINING ,MEDICAL protocols ,MEDICAL statistics ,CANCER cells - Abstract
Human adipose-derived stem cells (hADSCs) may provide a suitable number of progenitors for the treatment of lymphatic edema; however, to date the protocols for inducing hADSCs into this tissue type have not been standardized. We wished to investigate the induction of hADSCs into lymphatic endothelial-like cells using vascular endothelial growth factor-C156S (VEGF-C156S) and other growth factors in vitro. hADSCs from healthy adult adipose tissue were purified using enzyme digestion. Differentiation was induced using medium containing VEGF-C156S and bovine fibroblast growth factor (bFGF). Differentiation was confirmed using immunostaining for lymphatic vessel endothelial hyaluronan receptor (LYVE-1) and fms-related tyrosine kinase 4 (FLT-4), two lymphatic endothelial cell markers. The expression levels of LYVE-1, prospero homeobox 1 (PROX-1), and FLT-4 throughout induction were assessed using reverse transcriptase quantitative polymerase chain reaction. hADSCs were successfully obtained by trypsin digest and purification. Flow cytometry showed these cells were similar to mesenchymal stem cells, with a high positive rate of CD13, CD29, CD44, and CD105, and a low positive rate of CD31, CD34, CD45, and HLA-DR. Induction to lymphatic endothelial-like cells was successful, with cells expressing high levels of LYVE-1, PROX-1, and FLT-4. Adipose-derived stem cells can be induced to differentiate into lymphatic endothelial-like cells using a medium containing VEGF-C156S, bFGF, and other growth factors. This population of lymphatic endothelial-like cells may be useful for lymphatic reconstruction in the future. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
9. Study on air-to-ground missile with strap-down imaging infrared seeker against moving target.
- Author
-
Li, Fu-gui, Xia, Qun-li, Xiong, lin-qing, and Yao, Yuan-xu
- Published
- 2013
10. Application of robust public-key watermarking in coal mining information construction process.
- Author
-
Hu Yan-Jun, Cao Xin-De, Li Peng, and Li Fu-Gui
- Published
- 2009
- Full Text
- View/download PDF
11. PPARγ suppression inhibits adipogenesis but does not promote osteogenesis of human mesenchymal stem cells
- Author
-
Yu, Wei-Hua, Li, Fu-Gui, Chen, Xiao-Yong, Li, Jian-Tao, Wu, Yan-Heng, Huang, Li-Hua, Wang, Zhen, Li, Panlong, Wang, Tao, Lahn, Bruce T., and Xiang, Andy Peng
- Subjects
- *
MESENCHYMAL stem cells , *ALKALINE phosphatase , *PEROXISOME proliferator-activated receptors , *ADIPOGENESIS , *BONE growth , *LENTIVIRUSES - Abstract
Abstract: Mesenchymal stem cells (MSCs) are the common progenitors of osteoblasts and adipocytes. A reciprocal relationship exists between osteogenesis and adipogenesis in the bone marrow, and the identification of signaling pathways that stimulate MSC osteogenesis at the expense of adipogenesis is of great importance from the viewpoint of developing new therapeutic treatments for bone loss. The adipogenic transcription factor peroxisome proliferator-activated receptor γ (PPARγ) has been reported to play a vital role in modulating mesenchymal lineage allocation within the bone marrow compartment, stimulating adipocyte development at the expense of osteoblast differentiation. Hence, PPARγ may be a valuable target for drugs intended to enhance bone mass. However, little direct evidence is available for the role played by PPARγ in human mesenchymal lineage allocation. In this study, using human MSCs as an in vitro model, we showed that the two isoforms of PPARγ, PPARγ1 and PPARγ2, were differentially induced during hMSC adipogenesis, whereas only PPARγ1 was detected during osteogenesis. BADGE and GW9662, two potential antagonists of PPARγ, as well as lentivirus-mediated knockdown of PPARγ, inhibited hMSC adipogenesis but did not significantly affect osteogenesis. PPARγ knockdown did not significantly influence the expression level of the osteogenic transcription factor Runx2. Together, these results suggest that PPARγ is not the master factor regulating mesenchymal lineage determination in human bone marrow. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
- View/download PDF
12. Effect of Seeker Disturbance Rejection Rate Parasitic Loop on Line of Sight Rate Extraction.
- Author
-
LI Fu-gui, XIA Qun-li, CUI Xiao-xi, and QI Zai-kang
- Subjects
- *
ASTRONAUTICAL research , *TRANSFER functions , *CONTROL theory (Engineering) , *MATHEMATICAL functions , *MATHEMATICAL analysis - Abstract
To choose a reasonable line of sight rate extraction scheme of seeker in missile general design, a useful method is present based on analysis of characteristics of seeker disturbance rejection rate parasitic loop. A model of parasitic loop were established for a typical gimbaled seeker. Then using frequency domain methods the characteristic difference of disturbance rejection rate transfer function and parasitic loop as well as guidance system are analyzed for two kinds of extraction scheme. The effect of guidance parameters on the parasitic loop stability region is studied with Routh criteria. Using an infrared image seeker's parameters, simulation is done under typical flight condition to confirm the theoretical analysis. The study shows that the parasitic loop can change characteristics of guidance system, and line of sight rate extraction schemes also have essential effect on stability and stable performance of guidance system due to parasitic loop. To identify characteristics of seeker disturbance rejection rate is useful for determining line of sight rate extraction scheme. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
13. Microproteinuria for detecting calcineurin inhibitor-related nephrotoxicity after liver transplantation.
- Author
-
Li J, Liu B, Yan LN, Wang LL, Lau WY, Li B, Wang WT, Xu MQ, Yang JY, and Li FG
- Subjects
- Adult, Aged, Female, Humans, Immunosuppressive Agents therapeutic use, Kidney Function Tests, Liver Transplantation immunology, Male, Middle Aged, Young Adult, Calcineurin Inhibitors, Immunosuppressive Agents toxicity, Kidney Failure, Chronic blood, Kidney Failure, Chronic complications, Kidney Failure, Chronic etiology, Liver Transplantation adverse effects, Proteinuria chemically induced
- Abstract
Aim: To investigate whether microproteinuria could be used as an early and sensitive indicator to detect calcineurin inhibitor (CNI)-related nephrotoxicity after liver transplantation., Methods: All liver transplant recipients with normal serum creatinine (SCr) and detectable microproteinuria at baseline were included in this study. The renal function was monitored by the blood clearance of (99m)Tc-diethylenetriaminepentaacetic acid every 6 mo. Microproteinuria, SCr and blood urea nitrogen (BUN) were measured at entry and at subsequent follow-up visits. The patients were divided into different groups according to the mean values of glomerular filtration rate (GFR) at the follow-up time points: Group 1, GFR decreased from baseline by 0%-10%; Group 2, GFR decreased from baseline by 11%-20%; Group 3, GFR decreased from baseline by 21%-40%; Group 4, GFR decreased from baseline by > 40% and/or SCr was increasing., Results: A total of 143 patients were enrolled into this study (23 females and 120 males). The mean follow-up was 32 mo (range 16-36 mo). Downward trends in renal function over time were observed in the study groups. SCr and BUN increased significantly only in Group 4 patients (P < 0.001). beta2-microglobulin (beta2m) and alpha1-microglobulin (alpha1m) significantly increased with the subtle change of renal function in recipients who were exposed to CNI-based immunosuppression regimens. The reductions in GFR were closely correlated with elevated alpha1m (r(2) = -0.728, P < 0.001) and beta2m (r(2) = -0.787, P < 0.001)., Conclusion: beta2m and alpha1m could be useful as early and sensitive indicators of CNI-induced nephrotoxicity.
- Published
- 2009
- Full Text
- View/download PDF
14. Donor safety in adult living donor liver transplantation using the right lobe: single center experience in China.
- Author
-
Li FG, Yan LN, Zeng Y, Yang JY, Lin QY, Jiang XZ, and Liu B
- Subjects
- Adolescent, Adult, Alanine Transaminase blood, Aspartate Aminotransferases blood, China epidemiology, Donor Selection, Female, Follow-Up Studies, Hepatectomy methods, Humans, Length of Stay, Liver diagnostic imaging, Liver enzymology, Male, Middle Aged, Postoperative Complications epidemiology, Retrospective Studies, Time Factors, Tomography, X-Ray Computed, Hepatectomy adverse effects, Liver surgery, Liver Transplantation statistics & numerical data, Living Donors statistics & numerical data
- Abstract
Aim: To evaluate the safety of donors in adult living donor liver transplantation (LDLT) using the right lobe in a single liver transplantation center in China., Methods: We investigated retrospectively 52 living donor liver resections performed from October 2003 to July 2006. All patients were evaluated by blood tests and abdominal CT. The mean donor age was 28.2 +/- 7.4 years. Residual liver volume was 42.1% +/- 4.7%. Mean operative time was 420 +/- 76.2 min; mean ICU stay, less than 36 h; mean hospital stay, 16.4 +/- 8.6 d; and mean follow-up period, 6 mo., Results: There was no mortality. The overall complication rate was 40% (21 donors). Major complications included biliary leak in two, and pneumonia in 2 donors. Minor complications included mild pleural effusion in 12 donors, transient ascites in 6, mild depression in 4, intra-abdominal collections in 2, and wound infections in 1 donor. Residual liver volume did not affect the complication rate. None required re-operation. Return to pre-donation activity occurred within 5-8 wk., Conclusion: Right hemi-hepatectomy can be performed safely with minimal risk in cases of careful donor selection. Major complications occurred in only 7.7% of our series.
- Published
- 2007
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.