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This work is an exploration into visualizing measurement of putrescine specific adsorption by photographing colorized molecule imprinting chromogenic hydrogel. Membranes are prepared with imitate templates 1,4-butylene glycol, adipic acid or succinic acid, respectively and ninhydrin is used as chromogenic agent for target molecule putrescine. The adsorbing concentration on MIP is reflected in the form of visible violet-colored stain differed in shape and shade, which are recorded by hyper spectral camera and proceeded into intuitive 3D isohypse surfaces plot. By quantifying the height of surface peaks, imprinting efficiency is estimated in accordance with polyvinyl alcohol concentration and crosslinking degree. The imprinting efficiency of three imitate molecule templates is also discussed. [ABSTRACT FROM AUTHOR]

Bovine serum albumin imprinted polyethylene glycol 600 (PEG600) grafted Calcium alginate (CaA) hydrogel microspheres were prepared and characterized. The adsorption and recognition properties of PEG600 grafted calcium alginate (CaA- g-PEG600) microspheres were evaluated and the results showed that the crosslinking structure of CaA- g-PEG600 microspheres exerted an obvious effect on the adsorption capacity and imprinting properties for bovine serum albumin. The adsorption isotherms and recognition properties indicated that the imprinted modified microspheres had excellent rebinding affinity toward target proteins and the imprinting efficiency varied according to PEG600 grafting degree. The adsorption capacity and the imprinting factor were 5.5 mg g and 3.6, respectively. Adsorption kinetics of CaA- g-PEG600 microspheres in accordance with the molecular weight between crosslinks ( Mc) was investigated and the structural influence on protein selective rebinding was discussed. Furthermore, the binary solution separation performance of CaA- g-PEG600 microspheres with different Mc was investigated by selective binding bovine serum albumin from protein mixture solution. [ABSTRACT FROM AUTHOR]

Electrospinning is an important tool for generating continuous fibers with large surface area and high pore ratio. The combination of nanofiber membrane and molecular imprinting can effectively measure specific adsorption of putrescine with coloring procedure. In this work, molecularly imprinted electrospun chromogenic membrane was synthesized with 1,4‐butanediol (BDO) as imitate template. Poly(vinyl alcohol) (PVA) is used as spinning solution and ninhydrin as chromogenic agent for visualizing detection of target molecular putrescine. The adsorption concentration on molecularly imprinted polymer is illustrated by blue‐purple colored stains and recorded by interactive three‐dimensional surface plot of ImageJ. The results indicated that a uniform and bead‐free PVA nanofiber was obtained using 10% (w/w) PVA solution, 15 kV voltage, 15 cm spinning distance, and 600 rpm roller speed. Also, the adsorption performance of molecular imprinting chromogenic membrane was investigated in detail, showing a higher adsorption capacity of 33.16 μg cm−2 and maximum imprinting efficiency of 2.30 under the best recognition conditions of 315 μL ninhydrin, 0.25% (w/w) BDO, and 0.4 cm sensing distance, as well as a short adsorption equilibrium time of 40 min. In addition, three amine competitors were discussed to reveal the better selectivity and higher specific recognition ability in the imprinted membrane. HIGHLIGHTS: The molecular imprinting and coloring structure is constructed in nanofiber membrane with an enhanced specific surface area.The adsorption on imprinted membrane is visualized by chromogenic reaction between putrescine and ninhydrin to form a three‐dimensional interactive surface proceeded using the ImageJ software.The imprinted nanofiber membrane with large area and high pore ratio is applied in detecting target in trace concentration within shortened time. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 48186. [ABSTRACT FROM AUTHOR]