1. In vitro toxicity of nitrite on haemocytes of the tiger shrimp, Penaeus monodon, using flow cytometric analysis
- Author
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Xian, Jian-An, Wang, An-Li, Hao, Xuan-Ming, Miao, Yu-Tao, Li, Bin, Ye, Chao-Xia, and Liao, Shao-An
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PHYSIOLOGICAL effects of nitrites , *BLOOD cells , *PENAEUS japonicus , *PENAEUS monodon , *FLOW cytometry , *REACTIVE oxygen species , *PHYSIOLOGICAL effects of nitrogen oxides , *ESTERASES - Abstract
Abstract: This study investigated the in vitro effects of nitrite on reactive oxygen species (ROS) production, NO production, esterase activity and cell apoptosis of Penaeus monodon haemocytes. Haemocytes were in vitro exposed to different dose of nitrite (0, 0.1, 0.5, 1, 5 and 10μM). Cellular responses of nitrite-treated haemocytes were determined by flow cytometry. The results revealed that haemocytes treated by nitrite in vitro showed conspicuous time- and dose-dependent decreases in ROS and NO production as well as esterase activity. Additionally, 0.1 and 0.5μM nitrite did not affect the apoptotic cell ratio during the 3h experimental time, while significant increases in apoptotic cells were observed after haemocyte exposure to nitrite at 1μM for 3h, and at 5 or 10μM for 1h. These results indicated that nitrite suppresses cellular functions, including production of ROS and NO, and activity of esterase. Cell apoptosis of haemocytes would be induced by extracellular nitrite as doses exceed 1μM. [Copyright &y& Elsevier]
- Published
- 2012
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