1. Panax Notoginseng Saponins Protect H9c2 Cells From Hypoxia-reoxygenation Injury Through the Forkhead Box O3a Hypoxia-inducible Factor-1 Alpha Cell Signaling Pathway
- Author
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Jing-Jing Liu, Xin-Wen Liu, Yong-Ping Fu, Hui-Ting Zhong, and Meng-Kai Lu
- Subjects
autophagy ,PNS ,HIF-1α ,Panax notoginseng ,Myocardial Reperfusion Injury ,Cell Line ,Mitochondrial Proteins ,chemistry.chemical_compound ,Hypoxia-Inducible Factor 1-Alpha ,Animals ,LY294002 ,Myocytes, Cardiac ,FOXO3a ,PI3K/AKT/mTOR pathway ,Pharmacology ,chemistry.chemical_classification ,Reactive oxygen species ,biology ,Cell growth ,Plant Extracts ,Autophagy ,Forkhead Box Protein O3 ,apoptosis ,Membrane Proteins ,Cardiovascular Agents ,Saponins ,biology.organism_classification ,Hypoxia-Inducible Factor 1, alpha Subunit ,Cell biology ,Rats ,chemistry ,Apoptosis ,Original Article ,Phosphatidylinositol 3-Kinase ,Cardiology and Cardiovascular Medicine ,Reactive Oxygen Species ,Proto-Oncogene Proteins c-akt ,Signal Transduction - Abstract
Panax notoginseng saponins (PNS) are commonly used in the treatment of cardiovascular diseases. Whether PNS can protect myocardial ischemia-reperfusion injury by regulating the forkhead box O3a hypoxia-inducible factor-1 alpha (FOXO3a/HIF-1α) cell signaling pathway remains unclear. The purpose of this study was to investigate the protective effect of PNS on H9c2 cardiomyocytes through the FOXO3a/HIF-1α cell signaling pathway. Hypoxia and reoxygenation of H9C2 cells were used to mimic MIRI in vitro, and the cells were treated with PNS, 2-methoxyestradiol (2ME2), and LY294002.” Cell proliferation, lactate dehydrogenase, and malonaldehyde were used to evaluate the degree of cell injury. The level of reactive oxygen species was detected with a fluorescence microscope. The apoptosis rate was detected by flow cytometry. The expression of autophagy-related proteins and apoptosis-related proteins was detected by western blot assay. PNS could reduce H9c2 hypoxia-reoxygenation injury by promoting autophagy and inhibiting apoptosis through the HIF-1α/FOXO3a cell signaling pathway. Furthermore, the protective effects of PNS were abolished by HIF-1α inhibitor 2ME2 and PI3K/Akt inhibitor LY294002. PNS could reduce H9c2 hypoxia-reoxygenation injury by promoting autophagy and inhibiting apoptosis through the HIF-1α/FOXO3a cell signaling pathway.
- Published
- 2021