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Your search keyword '"Zhang, Mingqing"' showing total 2 results
Start Over Author "Zhang, Mingqing" Topic daphnia carinata Language english
2 results on '"Zhang, Mingqing"'

2. Cloning, expression and cellular localization of the Doublesex gene in the water flea, Daphnia carinata, during different developmental stages.

Author

Zhang, Mingqing, Li, Haixia, Liu, Ajing, Wu, Donglei, Wang, Danli, and Zhao, Yunlong

Subjects
*MOLECULAR cloning, *GENE expression, *DOUBLESEX gene, *CLADOCERA, *INSECT development, *GENE amplification, *SEX differentiation (Embryology)
Abstract

In this study, one of Doublesex genes from the common freshwater cladoceran Daphnia carinata , designated DapcaDsx1 , was cloned using primers based on homologous sequences and rapid amplification of cDNA ends (RACE). qPCR was employed to quantify differences in DapcaDsx1 expression between the different sexual phases, with expression levels being higher in sexual females. The role of DapcaDsx1 in the reproductive transformation was further investigated in parthenogenetic-phase females and sexual-phase females using whole-mount in situ hybridization. This cellular localization study showed specific expression of DapcaDsx1 in the thoracic segments, second antenna and part of the ventral carapace. Higher expression levels were exhibited in sexual females compared to parthenogenetic females. This suggests that the DapcaDsx1 gene plays significant roles in switching modes of reproduction and during sexual differentiation. [ABSTRACT FROM AUTHOR]

Published
2014
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