1. Optimization of liquid fermentation of microbial consortium WSD-5 followed by saccharification and acidification of wheat straw.
- Author
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Wen B, Yuan X, Cao Y, Liu Y, Wang X, and Cui Z
- Subjects
- Anaerobiosis, Biodegradation, Environmental, Biodiversity, Denaturing Gradient Gel Electrophoresis, Enzymes metabolism, Polymerase Chain Reaction, RNA, Ribosomal genetics, Substrate Specificity, Acids chemistry, Biotechnology methods, Carbohydrate Metabolism, Fermentation physiology, Microbial Consortia genetics, Triticum chemistry, Waste Products analysis
- Abstract
The microbial consortium WSD-5 is composed of bacteria and fungi, and the cooperation and symbiosis of the contained microbes enhance the degradation ability of WSD-5. Experiment results showed that the highest cellulase and hemicellulase were obtained when ventilation volume was 4 L/min, stirring rate was 0 rpm, and substrate loading rate was 3%. After 6 days of cultivation, a 67.60% loss in wheat straw dry weight was observed. The crude enzyme secreted from WSD-5 after optimization was evaluated by experiments of saccharification and acidification. The maximum concentration of reducing sugars was 3254 mg/L after 48 h saccharification. The concentration of sCOD peaked on day 2 with a value of 4345 mg/L during acidification, and the biogas yield and methane yield were 22.3% and 32.3% higher than un-acidified samples. This study is the first attempt to explore both the saccharification and the acidification ability of crude enzymes secreted by microbial consortium., (Copyright © 2012 Elsevier Ltd. All rights reserved.)
- Published
- 2012
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