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[Display omitted] • A novel ratio fluorescence probe of BN-L-CDs@[Ru(bpy) 3 ]2+ was designed. • The ratio fluorescent probes could sensitively detect ATP and CK. • The mechanism is that CK converts ATP to ADP while catalyzing Cr to CrP. • Smart phone platform could distinguish subtle colors for precise detection. Creatine kinase (CK), as an important biochemical index of cardiac injury, is very significant for early and accurate diagnosis of acute myocardial infarction or cardiac arrest. However, the sensitivity and specificity of CK detection still face great challenges. Herein, a ratio fluorescence probe was designed and prepared by electrostatic interaction between boron and nitrogen doped carbon dots (BN-L-CDs) as a response signal and Ru(bpy) 3 Cl 2 ·6H 2 O as independent reference signal. Notably, the BN-L-CDs@[Ru(bpy) 3 ]2+ displayed an ultra-fast fluorescence response to CK in ATP medium and an excellent linear relationship was observed between the concentration of CK and the fluorescence recovery ratio with a detection limit as low as 1.20 U/L. Mechanism investigates demonstrated that the "ON-OFF-ON" progress could be attributed to the formation of the non-luminescent ground state complex BN-L-CDs-ATP by the interaction of BN-L-CDs with ATP and the dissociation of complex under the catalysis of CK, respectively. Besides, the ratio fluorescence probe was further applied to living cell imaging due to its excellent biocompatibility. Importantly, a portable visual sensing platform was developed by combining a ratio fluorescence probe with a mobile phone color recognition application. Therefore, this new sensor provided a new perspective for the convenient monitoring of CK and paved the way for the relevant applications of clinical diagnostics. [ABSTRACT FROM AUTHOR]

Potassium ferricyanide (K 4 [Fe(CN) 6 ]) as anti-caking agent plays an important role in avoiding the formation of chunks for fine particulate solids. However, inappropriate and excessive addition and decomposition of K 4 [Fe(CN) 6 ] are detrimental to physical health. At present, appropriate strategies for convenient and accurate analysis of K 4 [Fe(CN) 6 ] in table salt and pickled food are desirable. Herein, an efficient "ON-OFF-ON" fluorescent sensor based on chiral carbon dots was prepared by a simple one-step hydrothermal method. The chiral CDs with L-Tryptophan and D-Tryptophan as chiral source were named as L-CDs and D-CDs. Notably, the bright fluorescence of L/D-CDs could be effectively quenched by K 4 [Fe(CN) 6 ] through dynamic quenching mechanism. This fluorescent sensor achieved excellent sensitive and selective detection of K 4 [Fe(CN) 6 ] with a limit of detection (LOD) of 25.0 ng·mL−1. In addition, the L/D-CDs could be applied not only for selective fluorescent recognition of K 4 [Fe(CN) 6 ] by the methods of portable filter paper and hydrogels, but also as fluorescent dye for repeated message encryption and decryption. [Display omitted] • An efficient "ON-OFF-ON" fluorescent sensor based on chiral carbon dots was prepared. • This method has high sensitivity and excellent selectivity for detecting K 4 [Fe(CN) 6 ]. • The detection limit is much lower than those of other methods. • The L-CDs have been successfully applied to solid state sensors and multiple information encryption. Potassium ferricyanide (K 4 [Fe(CN) 6 ]) as anti-caking agent plays an important role in avoiding the formation of chunks for fine particulate solids. However, inappropriate and excessive addition and decomposition of K 4 [Fe(CN) 6 ] are detrimental to physical health. At present, appropriate strategies for convenient and accurate analysis of K 4 [Fe(CN) 6 ] in table salt and pickled food are desirable. Herein, an efficient "ON-OFF-ON" fluorescent sensor based on chiral carbon dots was prepared by a simple one-step hydrothermal method. The chiral CDs with L-Tryptophan and D-Tryptophan as chiral source were named as L-CDs and D-CDs. Notably, the bright fluorescence of L/D-CDs could be effectively quenched by K 4 [Fe(CN) 6 ] through dynamic quenching mechanism. This fluorescent sensor achieved excellent sensitive and selective detection of K 4 [Fe(CN) 6 ] with a limit of detection (LOD) of 25.0 ng·mL−1. In addition, the L/D-CDs could be applied not only for selective fluorescent recognition of K 4 [Fe(CN) 6 ] by the methods of portable filter paper and hydrogels, but also as fluorescent dye for repeated message encryption and decryption. [ABSTRACT FROM AUTHOR]

One-pot synthesis of N -CDs was applied as a highly efficient "ON-OFF-ON" fluorescent probe for the rapid detection of CAL and l -Arg/ l -Lys in living cells. As a consequence, these N -CDs as a powerful analysis tool displayed more prominent and influential ability in real-time monitoring CAL and l -Arg/ l -Lys, and might offer a new opportunity for exploring the physiological and pathological processes of amino acid metabolism diseases. [Display omitted] • The nitrogen-doped carbon dots (N -CDs) were synthesized via a simple one-step hydrothermal method. • These CDs could rapidly and selectively detect CAL and l -Arg/ l -Lys with the detection limits as low as 58 nM, 18 nM/16 nM, respectively. • The N -CDs as a fluorescent probe displayed low toxicity and were suitable for sensing CAL and l -Arg/ l -Lys in living cells. The disorder of amino acid metabolism and the abuse of small molecule drugs pose serious threats to public health. However, due to the limitations of existing detection technologies in sensing cinnamaldehyde (CAL) and l -Arginine/ l -Lysine (l -Arg/ l -Lys), there is an urgent need to develop new sensing strategies to meet the severe challenges currently facing. Herein, nitrogen-doped carbon dots (N -CDs) were developed using a simple one-pot hydrothermal carbonization method. These N -CDs exhibited numerous distinctive characteristics such as excellent photoluminescence, high water dispersibility, favorable biocompatibility, and superior chemical inertness. Strikingly, the as-prepared CDs as a highly efficient fluorescent probe possessed significant sensitivity and selectivity toward CAL and l -Arg/ l -Lys over other analytes with a low detection limit of 58 nM and 16 nM/18 nM, respectively. The fluorescence of N -CDs could be quenched by CAL through an electron transfer process. Then, the strong electrostatic interaction between l -Arg/ l -Lys and N -CDs induced the efficient fluorescence recovery. More importantly, the outstanding biosafety and excellent analyte-responsive fluorescence characteristics of N -CDs have also been verified in living cells as well as in serum and urine. Overall, the N -CDs had a wide application prospect in the diagnosis of amino acid metabolic diseases and small molecule drug sensing. [ABSTRACT FROM AUTHOR]

One-pot synthesis of N -CDs was applied as a highly efficient "ON-OFF-ON" fluorescent probe for the rapid detection of CAL and l -Arg/ l -Lys in living cells. As a consequence, these N -CDs as a powerful analysis tool displayed more prominent and influential ability in real-time monitoring CAL and l -Arg/ l -Lys, and might offer a new opportunity for exploring the physiological and pathological processes of amino acid metabolism diseases. [Display omitted] • The nitrogen-doped carbon dots (N -CDs) were synthesized via a simple one-step hydrothermal method. • These CDs could rapidly and selectively detect CAL and l -Arg/ l -Lys with the detection limits as low as 58 nM, 18 nM/16 nM, respectively. • The N -CDs as a fluorescent probe displayed low toxicity and were suitable for sensing CAL and l -Arg/ l -Lys in living cells. The disorder of amino acid metabolism and the abuse of small molecule drugs pose serious threats to public health. However, due to the limitations of existing detection technologies in sensing cinnamaldehyde (CAL) and l -Arginine/ l -Lysine (l -Arg/ l -Lys), there is an urgent need to develop new sensing strategies to meet the severe challenges currently facing. Herein, nitrogen-doped carbon dots (N -CDs) were developed using a simple one-pot hydrothermal carbonization method. These N -CDs exhibited numerous distinctive characteristics such as excellent photoluminescence, high water dispersibility, favorable biocompatibility, and superior chemical inertness. Strikingly, the as-prepared CDs as a highly efficient fluorescent probe possessed significant sensitivity and selectivity toward CAL and l -Arg/ l -Lys over other analytes with a low detection limit of 58 nM and 16 nM/18 nM, respectively. The fluorescence of N -CDs could be quenched by CAL through an electron transfer process. Then, the strong electrostatic interaction between l -Arg/ l -Lys and N -CDs induced the efficient fluorescence recovery. More importantly, the outstanding biosafety and excellent analyte-responsive fluorescence characteristics of N -CDs have also been verified in living cells as well as in serum and urine. Overall, the N -CDs had a wide application prospect in the diagnosis of amino acid metabolic diseases and small molecule drug sensing. [ABSTRACT FROM AUTHOR]