1. Genetically modified rice seeds accumulating GLP-1 analogue stimulate insulin secretion from a mouse pancreatic beta-cell line.
- Author
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Sugita K, Endo-Kasahara S, Tada Y, Lijun Y, Yasuda H, Hayashi Y, Jomori T, Ebinuma H, and Takaiwa F
- Subjects
- Animals, Base Sequence, Cell Line, Genetic Vectors genetics, Globulins genetics, Globulins metabolism, Glucagon genetics, Glucagon-Like Peptide 1, Insulin Secretion, Islets of Langerhans cytology, Mice, Oryza genetics, Peptide Fragments genetics, Protein Precursors genetics, Recombinant Fusion Proteins genetics, Recombinant Fusion Proteins metabolism, Recombinant Fusion Proteins pharmacology, Seeds genetics, Glucagon metabolism, Glucagon pharmacology, Insulin metabolism, Islets of Langerhans drug effects, Islets of Langerhans metabolism, Oryza metabolism, Peptide Fragments metabolism, Peptide Fragments pharmacology, Protein Precursors metabolism, Protein Precursors pharmacology, Seeds metabolism
- Abstract
Glucagon-like peptide-1 (7-36) amide (GLP-1) is the most potent physiological insulinotropic hormone in humans. We produced large amounts of a GLP-1 analogue, [Ser8, Gln26, Asp34]-GLP-1, which is resistant to trypsin-digestion, as part of a chimeric rice seed storage protein, a 26 kDa globulin, in genetically modified rice seeds. Junction sites between GLP-1 analogue and globulin were replaced by tryptic cleavage sites. The highest level of GLP-1 analogue accumulation was approximately 20-50 microg per seed. We found that GLP-1 analogue derived from trypsin-digested genetically modified rice seeds stimulated insulin secretion from a mouse pancreatic beta-cell line, MIN6.
- Published
- 2005
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