1. Haemocyte Apoptosis of the Tiger Shrimp Penaeus monodon Exposed to Cadmium.
- Author
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Xian, Jian-An, Li, Bin, Guo, Hui, Miao, Yu-Tao, Ye, Jian-Min, Feng, Li-Na, Ye, Chao-Xia, Pan, Xun-Bin, Wang, An-Li, and Hao, Xuan-Ming
- Subjects
BLOOD cells ,APOPTOSIS ,PENAEUS japonicus ,CADMIUM poisoning ,PHYSIOLOGICAL effects of cadmium ,FLOW cytometry ,REACTIVE oxygen species - Abstract
This study investigated the effect of ambient Cadmium (Cd) on haemocyte apoptosis of the shrimp, Penaeus monodon. Cellular response was determined in Cd-exposed (0, 0.05, 0.5 and 5 mg L) shrimp. Results showed that 0.05 mg L Cd had no significant effect on the haemocyte parameters during the 48 h exposure. Cadmium at doses of 0.5 and 5 mg L depressed the total haemocyte count (THC), and increased reactive oxygen species (ROS) production and apoptosis ratio in haemocytes. Esterase activity increased in shrimp exposed to 0.5 mg L Cd for 6 h, and decreased to the initial level later. Depressed esterase activity could be observed in shrimp after 24 and 48 h exposure to 5 mg L Cd. These results demonstrated that Cd modified esterase activity and induced ROS generation, which led to haemocyte apoptosis and THC reduction. Oxidative stress is one of the induction mechanisms for Cd-caused apoptosis of shrimp haemocytes. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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