1. Toward homeostasis: regulatory dendritic cells from the bone marrow of mice with inflammation of the airways and peritoneal cavity.
- Author
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Scott NM, Ng RL, Strickland DH, Bisley JL, Bazely SA, Gorman S, Norval M, and Hart PH
- Subjects
- Alum Compounds, Animals, B7-2 Antigen metabolism, Bone Marrow Cells drug effects, CD11c Antigen metabolism, Cell Differentiation drug effects, Cell Movement immunology, Cross-Priming immunology, Dendritic Cells drug effects, Dendritic Cells enzymology, Granulocyte-Macrophage Colony-Stimulating Factor pharmacology, Haptens immunology, Homeostasis drug effects, Hypersensitivity complications, Hypersensitivity immunology, Hypersensitivity pathology, Immunization, Indomethacin pharmacology, Inflammation complications, Inflammation immunology, Interleukin-4 pharmacology, Lung drug effects, Lung immunology, Lung Diseases complications, Lung Diseases immunology, Lung Diseases pathology, Lymph Nodes drug effects, Lymph Nodes pathology, Membrane Proteins pharmacology, Mice, Mice, Inbred BALB C, Myelopoiesis drug effects, Ovalbumin immunology, Bone Marrow Cells immunology, Bone Marrow Cells pathology, Dendritic Cells immunology, Homeostasis immunology, Inflammation pathology, Lung pathology, Peritoneal Cavity pathology
- Abstract
Inflammatory mediators from peripheral tissues may control dendritic cell (DC) development in the bone marrow. In this study, DCs (CD11c(+) cells) differentiated from the bone marrow of mice with inflammation of the airways, or the peritoneal cavity had poor priming ability resulting in reduced, long-lived responses to that antigen in vivo. This indicates enhancement of regulatory mechanisms of immune responses through a peripheral tissue-bone marrow axis. If CD11c(+) cells, expanded from the bone marrow of mice with tissue inflammation were antigen pre-loaded and injected into mice already sensitized to that antigen, then subsequent contact hypersensitivity responses were significantly reduced. The effects of inflammation were imprinted in vivo and were independent of in vitro culture conditions for DC differentiation. The effect of tissue inflammation on the bone marrow DC precursors was not detected in mice treated subcutaneously with slow-release indomethacin pellets, suggesting a role for prostanoids, including prostaglandin E(2), in differentiation of regulatory CD11c(+) cells from bone marrow. Our study represents an important homeostatic process with potential for therapeutic use in the future., (Copyright © 2012 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.)
- Published
- 2012
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