1. Porous composite scaffolds of hydroxyapatite/silk fibroin via two-step method.
- Author
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Liu, Lin, Liu, Jinying, Kong, Xiangdong, Cai, Yurong, and Yao, Juming
- Subjects
HYDROXYAPATITE ,COMPOSITE materials research ,BIOCOMPATIBILITY ,POROSITY ,TISSUE engineering - Abstract
A two-step method was used to fabricate the hydroxyapatite (HAP)/silk fibroin (SF) scaffolds, i.e. the nano-sized HAP/SF composite powders were prepared by co-precipitation, which were then blended with SF solution to fabricate the HAP/SF composite scaffolds. The obtained scaffolds showed a 3D porous structure. The porosity was higher than 90% with the average macropore size of 214.2 µm. Moreover, the nano-sized HAP/SF composite powders were uniformly dispersed in the silk fibroin matrix, which provided the scaffolds enhanced compressive properties. The cell culture assay showed that the scaffolds fabricated by the two-step method could improve the cell proliferation and osteogenic differentiation when compared with those prepared by the conventional one-step blending method. The results suggested that the two-step method could promote the uniform dispersion of HAP in the SF matrix and efficient combination between the HAP and the matrix, which may provide a potential application in the composite scaffold preparation for tissue engineering. Copyright © 2009 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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