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Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:40:50Z No. of bitstreams: 1 S1516-89132006000300002.pdf: 70841 bytes, checksum: 62f2a3f4ab69dfee30b7cff44ce2e09f (MD5) Made available in DSpace on 2013-08-22T18:40:50Z (GMT). No. of bitstreams: 1 S1516-89132006000300002.pdf: 70841 bytes, checksum: 62f2a3f4ab69dfee30b7cff44ce2e09f (MD5) Previous issue date: 2006-03-01 Made available in DSpace on 2013-09-30T19:26:59Z (GMT). No. of bitstreams: 2 S1516-89132006000300002.pdf: 70841 bytes, checksum: 62f2a3f4ab69dfee30b7cff44ce2e09f (MD5) S1516-89132006000300002.pdf.txt: 29552 bytes, checksum: be02c05efaa7808d4998bbef255392b5 (MD5) Previous issue date: 2006-03-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:07:31Z No. of bitstreams: 2 S1516-89132006000300002.pdf: 70841 bytes, checksum: 62f2a3f4ab69dfee30b7cff44ce2e09f (MD5) S1516-89132006000300002.pdf.txt: 29552 bytes, checksum: be02c05efaa7808d4998bbef255392b5 (MD5) Made available in DSpace on 2014-05-20T15:07:31Z (GMT). No. of bitstreams: 2 S1516-89132006000300002.pdf: 70841 bytes, checksum: 62f2a3f4ab69dfee30b7cff44ce2e09f (MD5) S1516-89132006000300002.pdf.txt: 29552 bytes, checksum: be02c05efaa7808d4998bbef255392b5 (MD5) Previous issue date: 2006-03-01 O trabalho avaliou o enraizamento de estacas de Pinus caribaea var. hondurensis Morelet sob a ação de diferentes níveis de reguladores vegetais. As estacas foram feitas de brotações de 4 a 6cm de comprimento de mudas de P. caribaea var. hondurensis Morelet com corte bisel na base sendo as acículas basais eliminadas. A base das estacas foram submetidas aos tratamentos por 2 segundos com os seguintes tratamentos: 1- NAA 2000mg L-1; 2- NAA 4000mg L-1; 3- NAA 6000mg L-1; 4- NAA 2000mg L-1 + PBZ 100mg L-1; 5- NAA 4000mg L-1 + PBZ; 6- NAA 6000mg L-1 + PBZ; 7- IBA 2000mg L-1; 8- IBA 4000mg L-1; 9- IBA 6000mg L-1; 10-IBA 2000mg L-1 + PBZ; 11- IBA 4000mg L-1 + PBZ; 12- IBA 6000mg L-1 + PBZ e testemunha. Após os tratamentos as estacas foram plantadas em tubetes contendo 50% de palha de arroz carbonizada e 50% de vermiculita. As avaliações realizadas aos 60 dias após o plantio mostraram que estacas de P. caribaea tratadas com IBA levaram a maior porcentagem de estacas enraizadas que aquelas tratadas com NAA, sendo o mais efetivo, IBA a 4000mg L-1 em conjunto com 100mg L-1 de paclobutrazol. This work evaluated the rooting of Pinus caribaea var. hondurensis Morelet cuttings under the action of different levels of plant growth regulators. The cuttings consisted of 4-6 cm long shoots of P. caribaea var. hondurensis Morelet with their basal needles removed. The basal part of the cuttings were treated for 2 seconds with the following treatments: 1- NAA 2000mg L-1; 2- NAA 4000mg L-1; 3- NAA 6000mg L-1; 4- NAA 2000mg L-1 + PBZ 100mg L-1; 5- NAA 4000mg L-1 + PBZ; 6- NAA 6000mg L-1 + PBZ; 7- IBA 2000mg L-1; 8- IBA 4000mg L-1; 9- IBA 6000mg L-1; 10-IBA 2000mg L-1 + PBZ; 11- IBA 4000mg L-1 + PBZ; 12- IBA 6000mg L-1 + PBZ; and a control. After receiving the treatment, the cuttings were planted in tubes containing 50% carbonized rice hulls and 50% vermiculite. The evaluations, performed 60 days after planting, showed that P. caribaea var. hondurensis cuttings treated with IBA produced a higher percentage of rooted cuttings than those treated with NAA; the most effective treatment was IBA 4000mg L-1 plus 100mg L-1 paclobutrazol. Aracruz Celulose S.A. Centro de Pesquisa e Tecnologia International Paper do Brasil Ltda. Universidade Estadual Paulista Instituto de Biociências Departamento de Botânica Universidade Estadual Paulista Instituto de Biociências Departamento de Bioestatística Universidade Estadual Paulista Instituto de Biociências Departamento de Botânica Universidade Estadual Paulista Instituto de Biociências Departamento de Bioestatística

Background Drug susceptible clinical isolates of Candida albicans frequently become highly tolerant to drugs during chemotherapy, with dreadful consequences to patient health. We used RNA sequencing (RNA-seq) to analyze the transcriptomes of a CDR (Candida Drug Resistance) strain and its isogenic drug sensitive counterpart. Results RNA-seq unveiled differential expression of 228 genes including a) genes previously identified as involved in CDR, b) genes not previously associated to the CDR phenotype, and c) novel transcripts whose function as a gene is uncharacterized. In particular, we show for the first time that CDR acquisition is correlated with an overexpression of the transcription factor encoding gene CZF1. CZF1 null mutants were susceptible to many drugs, independently of known multidrug resistance mechanisms. We show that CZF1 acts as a repressor of β-glucan synthesis, thus negatively regulating cell wall integrity. Finally, our RNA-seq data allowed us to identify a new transcribed region, upstream of the TAC1 gene, which encodes the major CDR transcriptional regulator. Conclusion Our results open new perspectives of the role of Czf1 and of our understanding of the transcriptional and post-transcriptional mechanisms that lead to the acquisition of drug resistance in C. albicans, with potential for future improvements of therapeutic strategies.