8 results on '"He, Feng"'
Search Results
2. Stabilization and Ribosome Association of Unspliced Pre-mRNAs in a Yeast upf1 - Mutant
- Author
-
He, Feng, Peltz, Stuart W., Donahue, Janet L., Rosbash, Michael, and Jacobson, Allan
- Published
- 1993
3. Targeted sequencing identifies a novel SH2D1A pathogenic variant in a Chinese family: Carrier screening and prenatal genetic testing
- Author
-
Jun-Yu Zhang, Chenming Xu, Songchang Chen, Yu-Qian Xiang, Yinghua Shen, He-Feng Huang, Chunxin Chang, Yiyao Chen, Lanlan Zhang, and Shuyuan Li
- Subjects
Male ,0301 basic medicine ,Proband ,Genetic Screens ,Molecular biology ,Maternal Health ,Gene Identification and Analysis ,lcsh:Medicine ,Artificial Gene Amplification and Extension ,Biochemistry ,Polymerase Chain Reaction ,Sequencing techniques ,0302 clinical medicine ,Pregnancy ,Prenatal Diagnosis ,Medicine and Health Sciences ,DNA sequencing ,Signaling Lymphocytic Activation Molecule Associated Protein ,Frameshift Mutation ,lcsh:Science ,Immunodeficiency ,Sanger sequencing ,Genetics ,Multidisciplinary ,medicine.diagnostic_test ,Messenger RNA ,Obstetrics and Gynecology ,High-Throughput Nucleotide Sequencing ,Genomics ,Pedigree ,Nucleic acids ,030220 oncology & carcinogenesis ,Amniocentesis ,symbols ,Female ,Research Article ,Adult ,Immunology ,Prenatal diagnosis ,Frameshift mutation ,Immune Deficiency ,03 medical and health sciences ,symbols.namesake ,Genomic Medicine ,Diagnostic Medicine ,medicine ,Humans ,Genetic Testing ,RNA, Messenger ,Genetic testing ,Clinical Genetics ,business.industry ,lcsh:R ,Dideoxy DNA sequencing ,Biology and Life Sciences ,Human Genetics ,Reverse Transcriptase-Polymerase Chain Reaction ,medicine.disease ,Lymphoproliferative Disorders ,Nonsense Mediated mRNA Decay ,Research and analysis methods ,Molecular biology techniques ,030104 developmental biology ,Mutation ,Primary immunodeficiency ,Women's Health ,RNA ,Clinical Immunology ,lcsh:Q ,Clinical Medicine ,business - Abstract
X-linked lymphoproliferative disease type 1 (XLP1) is a rare primary immunodeficiency characterized by a clinical triad consisting of severe EBV-induced hemophagocytic lymphohistiocytosis, B-cell lymphoma, and dysgammaglobulinemia. Mutations in SH2D1A gene have been revealed as the cause of XLP1. In this study, a pregnant woman with recurrence history of birthing immunodeficiency was screened for pathogenic variant because the proband sample was unavailable. We aimed to clarify the genetic diagnosis and provide prenatal testing for the family. Next-generation sequencing (NGS)-based multigene panel was used in carrier screening of the pregnant woman. Variants of immunodeficiency related genes were analyzed and prioritized. Candidate variant was verified by using Sanger sequencing. The possible influence of the identified variant was evaluated through RNA assay. Amniocentesis, karyotyping, and Sanger sequencing were performed for prenatal testing. We identified a novel de novo frameshift SH2D1A pathogenic variant (c.251_255delTTTCA) in the pregnant carrier. Peripheral blood RNA assay indicated that the mutant transcript could escape nonsense-mediated mRNA decay (NMD) and might encode a C-terminal truncated protein. Information of the variant led to success prenatal diagnosis of the fetus. In conclusion, our study clarified the genetic diagnosis and altered disease prevention for a pregnant carrier of XLP1.
- Published
- 2017
4. Sequential expression of long noncoding RNA as mRNA gene expression in specific stages of mouse spermatogenesis
- Author
-
Hui Tian, Yulei Li, Fei Sun, Lu Wang, He-Feng Huang, Wenqing Li, Yu Lin, Meng Liang, and Tao Hu
- Subjects
Genetic Markers ,Male ,Biology ,Article ,Mice ,Spermatocytes ,Animals ,RNA, Messenger ,Spermatogenesis ,Regulation of gene expression ,Genetics ,Messenger RNA ,Multidisciplinary ,Microarray analysis techniques ,Gene Expression Profiling ,RNA ,Gene Expression Regulation, Developmental ,Microarray Analysis ,Spermatids ,Long non-coding RNA ,Spermatogonia ,Cell biology ,Gene expression profiling ,Mice, Inbred C57BL ,Adult Stem Cells ,RNA, Long Noncoding ,Biogenesis - Abstract
Many long noncoding RNA (lncRNA) species have been identified in gametes. However, the biogenesis and function of other categories of lncRNAs in gametes is poorly understood. Here, we profiled the expression of lncRNAs and mRNAs in spermatogonial stem cells (SSC), type A spermatogonia (A), pachytene spermatocytes (PS) and round spermatids (RS) by microarray analysis. We analyze the total expression of lncRNA/mRNA in these four germ cells and found that the maximum number of lncRNAs expression is in A (22127), and the minimum is in PS (14456). Also, the maximum number of mRNAs is in A (19923), and the minimum is in PS (13941). Furthermore, the trend in the number of specific lncRNAs was similar to the number of specific mRNAs in each type of germ cells (e.g., maximum in A and minimum in PS). The trend in the number of lncRNAs was similar to the number of mRNAs in two continued types of germ cells (e.g., maximum in SSC to A and minimum in PS to RS). The correlation analysis showed a high correlation coefficient of lncRNAs/mRNAs expression (R = 0.992). The results suggested that the sequential expression of long noncoding RNA as mRNA gene expression exhibits coordinated changes in male spermatogenesis.
- Published
- 2014
- Full Text
- View/download PDF
5. Conserved mRNA-granule component Scd6 targets Dhh1 to repress translation initiation and activates Dcp2-mediated mRNA decay in vivo.
- Author
-
Zeidan, Quira, Zhang, Fan, Zhang, Hongen, Hinnebusch, Alan G., He, Feng, and Jacobson, Allan
- Subjects
MESSENGER RNA ,PROTEINS ,RNA-protein interactions ,PROTEIN expression ,RIBOSOMES ,RNA sequencing - Abstract
Scd6 protein family members are evolutionarily conserved components of translationally silent mRNA granules. Yeast Scd6 interacts with Dcp2 and Dhh1, respectively a subunit and a regulator of the mRNA decapping enzyme, and also associates with translation initiation factor eIF4G to inhibit translation in cell extracts. However, the role of Scd6 in mRNA turnover and translational repression in vivo is unclear. We demonstrate that tethering Scd6 to a GFP reporter mRNA reduces mRNA abundance via Dcp2 and suppresses reporter mRNA translation via Dhh1. Thus, in a dcp2Δ mutant, tethered Scd6 reduces GFP protein expression with little effect on mRNA abundance, whereas tethered Scd6 has no impact on GFP protein or mRNA expression in a dcp2Δ dhh1Δ double mutant. The conserved LSm domain, but not the RGG domain, of Scd6 is required for translational repression and mRNA turnover by tethered Scd6. Both functions are enhanced in a ccr4Δ mutant, suggesting that the deadenylase function of Ccr4-Not complex interferes with a more efficient repression pathway enlisted by Scd6. Ribosome profiling and RNA-Seq analysis of scd6Δ and dhh1Δ mutants suggests that Scd6 cooperates with Dhh1 in translational repression and turnover of particular native mRNAs, with both processes dependent on Dcp2. Our results suggest that Scd6 can (i) recruit Dhh1 to confer translational repression and (ii) activate mRNA decapping by Dcp2 with attendant degradation of specific mRNAs in vivo, in a manner dependent on the Scd6 LSm domain and modulated by Ccr4. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
6. Genome-wide maps of ribosomal occupancy provide insights into adaptive evolution and regulatory roles of uORFs during Drosophila development.
- Author
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Zhang, Hong, Dou, Shengqian, He, Feng, Luo, Junjie, Wei, Liping, and Lu, Jian
- Subjects
DROSOPHILA development ,INSECT genomes ,RIBOSOMAL RNA ,ANIMAL life cycles ,NUCLEOTIDE sequence - Abstract
Upstream open reading frames (uORFs) play important roles in regulating the main coding DNA sequences (CDSs) via translational repression. Despite their prevalence in the genomes, uORFs are overall discriminated against by natural selection. However, it remains unclear why in the genomes there are so many uORFs more conserved than expected under the assumption of neutral evolution. Here, we generated genome-wide maps of translational efficiency (TE) at the codon level throughout the life cycle of Drosophila melanogaster. We identified 35,735 uORFs that were expressed, and 32,224 (90.2%) of them showed evidence of ribosome occupancy during Drosophila development. The ribosome occupancy of uORFs is determined by genomic features, such as optimized sequence contexts around their start codons, a shorter distance to CDSs, and higher coding potentials. Our population genomic analysis suggests the segregating mutations that create or disrupt uORFs are overall deleterious in D. melanogaster. However, we found for the first time that many (68.3% of) newly fixed uORFs that are associated with ribosomes in D. melanogaster are driven by positive Darwinian selection. Our findings also suggest that uORFs play a vital role in controlling the translational program in Drosophila. Moreover, we found that many uORFs are transcribed or translated in a developmental stage-, sex-, or tissue-specific manner, suggesting that selective transcription or translation of uORFs could potentially modulate the TE of the downstream CDSs during Drosophila development. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
7. Targeted sequencing identifies a novel SH2D1A pathogenic variant in a Chinese family: Carrier screening and prenatal genetic testing.
- Author
-
Zhang, Jun-Yu, Chen, Song-Chang, Chen, Yi-Yao, Li, Shu-Yuan, Zhang, Lan-Lan, Shen, Ying-Hua, Chang, Chun-Xin, Xiang, Yu-Qian, Huang, He-Feng, and Xu, Chen-Ming
- Subjects
LYMPHOPROLIFERATIVE disorders ,RARE diseases ,GENETIC testing ,B cell lymphoma ,CHINESE people ,NUCLEOTIDE sequence ,GENETIC mutation ,GENETICS ,DISEASES - Abstract
X-linked lymphoproliferative disease type 1 (XLP1) is a rare primary immunodeficiency characterized by a clinical triad consisting of severe EBV-induced hemophagocytic lymphohistiocytosis, B-cell lymphoma, and dysgammaglobulinemia. Mutations in SH2D1A gene have been revealed as the cause of XLP1. In this study, a pregnant woman with recurrence history of birthing immunodeficiency was screened for pathogenic variant because the proband sample was unavailable. We aimed to clarify the genetic diagnosis and provide prenatal testing for the family. Next-generation sequencing (NGS)-based multigene panel was used in carrier screening of the pregnant woman. Variants of immunodeficiency related genes were analyzed and prioritized. Candidate variant was verified by using Sanger sequencing. The possible influence of the identified variant was evaluated through RNA assay. Amniocentesis, karyotyping, and Sanger sequencing were performed for prenatal testing. We identified a novel de novo frameshift SH2D1A pathogenic variant (c.251_255delTTTCA) in the pregnant carrier. Peripheral blood RNA assay indicated that the mutant transcript could escape nonsense-mediated mRNA decay (NMD) and might encode a C-terminal truncated protein. Information of the variant led to success prenatal diagnosis of the fetus. In conclusion, our study clarified the genetic diagnosis and altered disease prevention for a pregnant carrier of XLP1. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
8. YRA1 Autoregulation Requires Nuclear Export and Cytoplasmic Edc3p-Mediated Degradation of Its Pre-mRNA
- Author
-
Dong, Shuyun, Li, Chunfang, Zenklusen, Daniel, Singer, Robert H., Jacobson, Allan, and He, Feng
- Subjects
- *
MESSENGER RNA , *NUCLEIC acids , *EUKARYOTIC cells , *RNA - Abstract
Summary: Autoregulatory loops often provide precise control of the level of expression of specific genes that encode key regulatory proteins. Here we have defined a pathway by which Yra1p, a yeast mRNA export factor, controls its own expression. We show that YRA1 exon 1 sequences in cis and Yra1p in trans inhibit YRA1 pre-mRNA splicing and commit the pre-mRNA to nuclear export. Mex67p and Crm1p jointly promote YRA1 pre-mRNA export, and once in the cytoplasm, the pre-mRNA is degraded by a 5′ to 3′ decay mechanism that is dependent on the decapping activator Edc3p and on specific sequences in the YRA1 intron. These results illustrate how common steps in the nuclear processing, export, and degradation of a transcript can be uniquely combined to control the expression of a specific gene and suggest that Edc3p-mediated decay may have additional regulatory functions in eukaryotic cells. [Copyright &y& Elsevier]
- Published
- 2007
- Full Text
- View/download PDF
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