1. Functional relationship between CgMyD88-1 and CgMyD88-2 in the Pacific oyster.
- Author
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Tang, Xueying, Huang, Baoyu, Lin, Siheng, Wang, Wei, Zhang, Guofan, and Li, Li
- Subjects
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PACIFIC oysters , *ADAPTOR proteins , *TRANSCRIPTION factors , *INVERTEBRATE diversity , *TOLL-like receptors - Abstract
MyD88 is a universal adapter protein for the Toll-like receptor/interleukin-1 receptor (TLR/IL-1R) signaling pathway. Since invertebrates are believed to lack MyD88-independent pathways, MyD88 appears more critical in oyster TLR signaling pathway. In the Pacific oyster (Crassostrea gigas), two complete paralogues, named as CgMyD88-1 and CgMyD88-2 , have been identified. In the current study, we indicated that CgMyD88-1 and CgMyD88-2 might act synergistically to increase the efficiency of immune signaling by activating NF-κB transcription factor. However, we found that upon stimulation with lipopolysaccharide (LPS) or polyinosinic:polycytidylic acid [poly (I:C)], CgMyD88-1 and CgMyD88-2 show differences in their response: CgMyD88-1 accumulated as large spots in the cytoplasm, while CgMyD88-2 assembled in the cytoplasm and in the membrane. Our results support the theory that expansion of these immune genes is associated with functional diversity. • CgMyD88-1 and CgMyD88-2 synergistically interact in CgTLR3-mediated immunity pathway. • CgMyD88-1 and CgMyD88-2 activate NF-κB transcription factor. • CgMyD88-1 and CgMyD88-2 show differential response upon LPS or poly (I:C) stimulation. • Expansion of MyD88 genes is associated with functional diversity in invertebrates. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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