1. Descriptor ΔG C-O Enables the Quantitative Design of Spontaneously Blinking Rhodamines for Live-Cell Super-Resolution Imaging.
- Author
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Chi W, Qiao Q, Wang C, Zheng J, Zhou W, Xu N, Wu X, Jiang X, Tan D, Xu Z, and Liu X
- Subjects
- Fluorescent Dyes metabolism, HeLa Cells, Humans, Microscopy, Fluorescence methods, Rhodamines metabolism
- Abstract
Herein, we reported a simple, fast, and quantitative theoretical descriptor ΔG
C-O that allows accurate predictions of a wide range of spontaneously blinking rhodamines. ΔGC-O denotes the Gibbs free energy differences between the closed and open forms of rhodamines and has a good linear relationship with experimental pKcycl values. This correlation affords an effective guide for the quantitative designs of spontaneously blinking rhodamines and eliminates trial-and-error. We have validated the predictive power of ΔGC-O via the development of two spontaneously blinking rhodamines of different colors and enhanced brightness. We also demonstrated their super-resolution imaging utilities in dynamic live-cell imaging. We expect that ΔGC-O will greatly facilitate the efficient creations of spontaneously blinking fluorophores and aid the advancements of super-resolution bioimaging techniques., (© 2020 Wiley-VCH GmbH.)- Published
- 2020
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