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Chromium (Cr) is a heavy metal that poses a grave threat to the ecosystem including plants. Chromium is very harmful to plants due to its effects on many physiological and metabolic pathways culminating in a negative impact on plant's growth, development, and ability to take up nutrients. Plants have developed physiological, biochemical, and molecular ways of defense against Cr, such as by augmenting antioxidant potential to reduce reactive oxygen species (ROS). A number of genes have been discovered to play a significant role in the defense mechanisms of plants against Cr, for example, genes associated with the activation of phytochelatins, metallothioneins, and those of enzymes like glutathione-S-transferases. Along with this, a few miRNAs have been found to be associated in alleviating Cr stress and, to augment plant tolerance by controlling transcription factors, HSPs, and the expression of a few proteins and hormones. Defense pathway genes and miRNAs have been used for the generation of transgenic phytoremediator plants. Not only do the transgenic plants have a higher tolerance to Cr, but they also act as hyperaccumulators for Cr and have the potential to remediate other heavy metals. This article describes about environmental Cr contamination, Cr effects on plants, different genes and miRNAs involved in Cr stress mitigation and use of candidate genes, microRNAs for creating transgenic plant systems for phytoremediation, and the applications of CRISPR technology. It is expected that the integration of omics approach and advanced genomics will offer scope for more effective phytoremediation of Chromium in the coming years., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

The presence of high concentrations of arsenic (As) in agricultural soils and its subsequent accumulation in rice crop is a serious issue threatening sustainability of agriculture and human health. In the present work, remediation of As contaminated field in Nadia, West Bengal, India was done through the cultivation of Vetiver (Vetiveria zizanoides L. Nash) and the same field was subsequently used for rice (Oryza sativa L.) cultivation. The results showed that V. zizanoides could reduce As concentrations in the field to bring it lower than the maximum permissible limit (20 mg kg -1 ) in 11 months' time. The rice plants grown in remediated field showed improvement in growth and photosynthesis parameters as compared to that of contaminated field. Importantly, yield related parameters (filled seed, 1000 grain weight, number of panicles etc.) were also significantly higher in remediated field than that in contaminated field. Arsenic concentration in roots, shoot, husk and grains of rice was found to be significantly lower in remediated field than in contaminated field. Grain As decreased from 0.75 to 0.77 μg g -1 dw in contaminated field to 0.15-0.18 μg g -1 dw. In conclusion, replacing rice for single year with V. zizanoides crop can significantly remediate the field and can be a viable option., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The present study analyzed the effects of individual microbes and their consortium (Priestia endophytica NDAS01F, Bacillus licheniformis NDSA24R, and P. flexa NDAS28R) either alone or in interaction with thiourea (TU) on growth and responses of rice plants subjected to As stress (50 mg kg -1 in soil) in a pot experiment. The bacteria used in the experiment were isolated from As contaminated fields of Nadia, West Bengal and showed significant As removal potential in in vitro experiment. The results revealed significant growth improvement, biomass accumulation, and decline in malondialdehyde levels in rice plants in bacterial and TU treatments as compared to control As treatment. The best results were observed in a bacterial consortium (B1-2-3), which induced a profound increase of 65%, 43%, 127% and 83% in root length, shoot length, leaf width and fresh weight, respectively. Sulfur metabolism and cell wall synthesis were stimulated upon bacterial and TU amendment in plants. The maximum reduction in As concentration was observed in B2 in roots (-55%) and in B1-2-3 in shoot (-83%). The combined treatment of B1-2-3 + TU proved to be less effective as compared to that of B1-2-3 in terms of As reduction and growth improvement. Hence, the usage of bacterial consortium obtained in the present work is a sustainable approach, which might find relevance in field conditions to achieve As reduction in rice grains and to attain higher growth of plants without the need for additional TU supplementation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Masson SAS. All rights reserved.)

Helianthus annuus is a potential metal accumulator plant, which can find application in cadmium (Cd) phytoremediation and provide economic gains in terms of oil yield. This study is focused on Cd accumulation analysis, physiological and biochemical responses of five varieties of H. annuus (DRSF-108, DRSF-113, LSFH-171, Phule Bhaskar and KBSH-44). Plantlets of all varieties were treated with various Cd concentrations (10, 50, 100, 300 and 500 mg kg -1 ) for 20, 40 and 60 days. DRSF-108 showed the maximum total Cd accumulation (430.52 mg kg -1 ) in whole plant while minimum accumulation was observed in KBSH-44 (150.66 mg kg -1 ) at 500 mg kg -1 Cd after 60 days. The highest level of proline and polyphenol in DRSF-108 were 27.206 µmol g -1 fw and 6.86 mg g -1 fw, respectively. Antioxidant enzymes (catalase, ascorbate peroxidase and glutathione reductase) also showed increased activity in response to Cd treatment. SEM-EDX analysis of potential accumulator genotype, DRSF-108, showed the distribution of intracellular Cd into plant tissues. Therefore, it is concluded that among five varieties, DRSF-108 was the most potential Cd accumulator and had a higher capacity for Cd tolerance compared to other varieties. Our findings may allow us to extend variety DRSF-108 for sustainable farming and Cd remediation.

Arsenic (As) is a major threat to the environment and human health due to its toxicity and carcinogenicity. Occurrence of alarming concentrations of As in water and soil leads to its bioaccumulation in crops which is a major health concern globally. Rice ( Oryza sativa ) is a staple food for a large population staying in As contaminated areas so, it is of utmost importance to reduce As levels in rice, especially grains. Amongst several strategies in practice, biotechnology may provide an effective option to reduce As accumulation in rice grains. Genetic engineering can be a viable approach to exploit potential genes playing roles in As metabolism pathway in plants. Besides, developing low As accumulating rice varieties through breeding is also an important area. Identifying genotypic variation in rice is a crucial step toward the development of a safe rice cultivar for growing in As-affected areas. Significant genotypic variation has been found in rice varieties for As accumulation in grains and that is attributable to differential expression of transporters, radial oxygen loss, and other regulators of As stress. This review provides recent updates on the research advances leading to transgenic and breeding approaches adopted to reduce As levels in rice, especially grains.

The present study analysed the levels of potentially toxic elements along with physico-chemical properties of agricultural soil samples (n = 59) collected from fields situated along the path of river Ganga in the middle Gangetic floodplain in two districts, Ballia and Ghazipur. Arsenic (As), chromium (Cr), copper (Cu), nickel (Ni), zinc (Zn), lead (Pb), iron (Fe) and manganese (Mn) levels were analysed by Wavelength Dispersive-X-Ray Fluorescence Spectroscopy (WD-XRF) and the associated health risks along with diverse indices were calculated. The mean concentrations of As, Cu, Cr, Pb, Zn and Ni were found to be 15, 42, 85, 18, 87 and 47 mg kg -1 , respectively in Ballia and 13, 31, 73, 22, 77 and 34 mg kg -1 , respectively in Ghazipur. Physico-chemical properties like pH, ORP and organic matter were found to be 7.91, 209 and 1.20, respectively in Ballia and 8.51, 155 and 1.25, respectively in Ghazipur. The calculated health quotient (HQ) for all the elements was observed to be within the threshold value of one, however with few exemptions. Therefore, the present study showcases the contamination of potentially toxic elements in agricultural fields and possible health hazards for people., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Thiourea (TU) is a chemo-priming agent and non-physiological reactive oxygen species (ROS) scavenger whose application has been found to reduce As accumulation in rice grains along with improved growth and yield. The present field study explored TU-mediated mechanistic changes in silicon (Si) assimilation in root/shoot, biochemical and molecular mechanisms of arsenic (As) stress amelioration in rice cultivars. Gosai and Satabdi (IET-4786) rice cultivars were selected for field experiment at three different places; control field and two other As contaminated experimental fields (EF1 and EF2) in West Bengal, India. The average As reduction was observed to be 9.5% and 19.8% whereas the yield increment was 8.8% and 17.7% for gosai and satabdi, respectively among all the three experimental fields. The positive interrelation was also observed between improved internal ultrastructure anatomy and enhanced Si assimilation (36%-423%) upon TU application. The level of photosynthetic pigments was increased by 29.8%-99.2%. Further, activities of antioxidant enzymes were harmonically altered in TU supplemented plants. The expression of various As related transporter genes in flag leaf and developing grains (inflorescence) was changed in both the rice cultivars (gosai and satabdi). It was also presumably responsible for observed As reduction in grains. Thus, TU application was found to be an efficient and sustainable agronomic practice for amelioration of As toxicity in rice plants in As contaminated field conditions., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

The present study assessed the utility of Allium cepa based cyto-genotoxicity bioassays in evaluating the arsenic toxicity and remediation potential of Pteris vittata on contaminated soil of Lakhimpur-Kheri district. Untreated and P. vittata treated soil extracts were used for cyto-genotoxicity tests in A. cepa. Results showed that P. vittata extracted high concentration of arsenic, which ranged from 220 to 1420 mgkg -1 in different soils. Cyto-genotoxic assessment of A. cepa showed that extract of P. vittata treated soil had lower cyto-genotoxic effects as compared to untreated soil. A higher mitotic index (10%) while lower mitotic depression (29%), relative abnormality rate (10%), chromosomal aberrations (1%) and micronuclei (2%) were detected in root meristematic cells of A. cepa exposed to remediated soil extract in comparison to untreated soil. The studies provide a simple, rapid and economic cyto-genotoxicity bioassay tool for evaluating toxicity of contaminated soils of contaminated soils as well as revealed the phytoremdiation property of P. vittata against arsenic toxicity., (© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

Arsenic (As) assessment in agricultural soils and corresponding crops is necessary from the global health safety perspective. To the best of our knowledge, we are reporting for the first time, As flux determining parametric equations for paddy field with seasonal rice cultivation under conventional flooding and dry-wet irrigation approaches. Rigorous field experiments and measuring quantitative parameters, flushed out or percolated into the deeper soil As flux was assessed. A wintery (boro)-monsoonal (aman) study from 2016 to 2019 has been conducted showing the efficiency of dry-wet irrigation on reduction of soil As bioavailability. The reduction in boro was 52.4% in 2016 to 64.8% in 2019 while in aman, it was 61% in 2016 to 74.9% in 2019. Low bioavailability was correlated to plant's internal vascular structure that was found more rigid and firm in dry-wet field grown plants. Observed soil physico-chemical parameters clearly influenced As bioavailability as well as soil microbial community. Assessment of microbial diversity using metagenomics under altered water regime was done by population analysis, relative abundance, species richness, Krona chart comparison. Dry-wet field was found to be more diverse and enriched in microbial community than that of the flooded soil indicating an affective reduction of As bioavailability under biotic-abiotic factors., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Arsenic (As) is a ubiquitous environmental carcinogen that enters the human food chain mainly through rice grains. In the present study, we evaluated the potential of thiourea (TU; non-physiological reactive oxygen species scavenger) in mitigating the negative effects of arsenic (As) stress in indica rice variety IR64, with the overall aim to reduce grain As accumulation. At seedling stage, As + TU treatment induced the formation of more numerous and longer crown roots compared with As alone. The As accumulation in main root, crown root, lower leaf and upper leaf was significantly reduced to 0.1-, 0.14-, 0.16-, 0.14-fold, respectively in As + TU treated seedlings compared with those of As alone. This reduced As accumulation was also coincided with light-dependent suppression in the expression levels of aquaporins and photosynthesis-related genes in As + TU treated roots. In addition, the foliar-supplemented TU under As-stress maintained reducing redox conditions which decreased the rate of As accumulation in flag leaves and, eventually grain As by 0.53-fold compared with those of As treatment. The agronomic feasibility of TU was validated under naturally As contaminated sites of Nadia (West Bengal, India). The tiller numbers and crop productivity (kg seed/ha) of TU-sprayed plants were increased by 1.5- and 1.18-fold, respectively; while, grain As accumulation was reduced by 0.36-fold compared with those of water-sprayed control. Thus, this study established TU application as a sustainable solution for cultivating rice in As-contaminated field conditions., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

The present study delineates the interactions of arsenic (As), a carcinogenic metalloid, and thiourea (TU), a non-physiological reactive oxygen species (ROS) scavenger, in rice plants grown in As contaminated fields in West Bengal, India. The study was performed for four consecutive seasons (two boro and two aman) in 2016 and 2017 with two local rice cultivars; Gosai and Satabdi (IET-4786) in a control and two As contaminated experimental fields. Thiourea (0.05% wt/vol) treatment was given in the form of seed priming and foliar spray. Thiourea significantly improved growth and yield of rice plants and reduced As concentration in root, shoot, husk and grains in both cultivars and fields. The reduction in As concentration ranged from 10.3% to 27.5% in four seasons in different fields. The average (four seasons) increase in yield was recorded about ~8.1% and ~11.5% in control, ~20.2% and ~18.6% in experimental field 1, and ~16.2% and ~24.1% in experimental field 2, for gosai and satabdi, respectively. Mean hazard quotient (HQ) and incremental lifetime cancer risk (ILCR) values of As reduced upon TU supplementation for both cultivars as compared to that of non-TU plants. Hence, TU can be effectively used to cultivate rice safely in As contaminated fields., (Copyright © 2020 Elsevier B.V. All rights reserved.)

The present experiment was designed to evaluate physico-chemical characteristics and phyto-genotoxicity of arsenic (As) contaminated soil collected from different sites of Lakhimpur, Uttar Pradesh (UP), India by employing Vicia faba L. The analyses included various biochemical as well as cyto-genotoxicity assays. The results showed that soil pH was slightly acidic to neutral in nature. The bulk density (1.18-1.23 gcm -3 ), particle density (2.51-2.59 gcm -3 ) and porosity (44-53%) varied in different places. The level of available nutrients, nitrogen, phosphorus and potassium was found to vary as 124-165 mgkg-1, 173-186 mgkg-1 and 48-98 mgkg-1, respectively. The maximum As levels were found in soil of Fulvareya (27.13 mgkg-1) and Atareya (24.12 mgkg-1), the level of As in water samples of these sites were 0.19 mgl -1 and 0.21 mgl -1 , respectively. Phytotoxicity of the As present in soils was evident through significant increases in stress metabolites, hydrogen peroxide (H 2 O 2 ), malondialdehyde (MDA) and carbonyl groups in root and shoot of V. faba. Cyto-genotoxic effects were also seen through reduced mitotic index (MI) and increased mitotic depression (MD), relative abnormality rate (RAR) as well as other chromosomal abnormalities along with micronuclei in root meristematic cells of V. faba. The phytotoxicity and cyto-genotoxicity assessment suggests the harmful soil properties that might affect biota., (Copyright © 2019. Published by Elsevier Ltd.)

The present study measured arsenic (As) concentrations in soil, groundwater and rice grain samples in two villages, Sarapur and Chinili, under Chakdaha block, Nadia district, West Bengal, India. This study also included a survey of the two villages to understand the knowledge among villagers about the As problem. Soil and groundwater samples were collected from fields in two villages while rice grain samples were collected from villagers' houses. The results revealed the presence of As in higher concentrations than the maximum permissible limit of As in drinking water (10 µg L -1 and 50 µg L -1 by WHO and Indian standard, respectively) in groundwater [124.50 ± 1.11 µg L -1 (Sarapur) and 138.20 ± 1.34 µg L -1 (Chinili)]. The level of As in soil was found to range from 47.7 ± 0.14 to 49.3 ± 0.19 mg Kg -1 in Sarapur and from 57.5 ± 0.25 to 62.5 ± 0.44 mg Kg -1 in Chinili which are also higher than European Union maximum acceptable limit in agricultural soil (i.e. 20 mg Kg -1 ). The analysis of As in rice grains of five varieties, collected from residents of two villages, showed the presence of higher than recommended safe level of As in rice by FAO/WHO (0.2 mg Kg -1 ). The As concentration order was Gosai (0.95 ± 0.044 mg kg -1 ), Satabdi (0.79 ± 0.038 mg kg -1 ), Banskathi (0.60 ± 0.026 mg kg -1 ), Kunti (0.47 ± 0.018 mg kg -1 ) and Ranjit (0.29 ± 0.021 mg kg -1 ). Importantly, Gosai and Satabdi were the most popular varieties being consumed by local people. The data of consumption of rice per day in the survey was used for the measurement of average daily dose and hazard quotient. It was seen that the As hazard was negatively correlated to the age of residents. Therefore, children and toddlers were at higher risk of As exposure than elderly people. In addition, people with skin related As toxicity symptoms were also cited in the two villages. The study emphasized the severity of As problem in remote areas of West Bengal, India where people consume As tainted rice due to lack of awareness about the As problem and associated health issues.

The study implements a periodical intermittent water cycle during rice cultivation providing insight potential in minimizing soil bio-available arsenic. Soil As concentrations were 34 ± 0.49 and 72.03 ± 0.54 mg kg-1 As respectively in two selected fields with rice cultivars gosai and satabdi, in comparison to 42.26 ± 0.37 and 83.69 ± 0.48 mg kg-1 in continuously flooded field soil, determined through ICP-MS. The study found higher translocation of silicon from soil to rice plant parts under intermittent irrigation having pH range of 7.6-9.4 and greater availability of soil organic content that in turn release more labile silicon from soil to aqueous phase for plant accumulation. This increased uptake of silicon strengthens rice shoots, nodes and leaf xylem-phloem integrity compared to conventional continuously flooded rice cultivation approach, suppressing the arsenic translocation, as observed under FE-SEM real-time imaging. Fresh plants were analysed for bioaccumulation and translocation factors of arsenic and silicon to justify the enhanced silicon uptake under proposed practice. Plant stress regulator enzymes viz. malondialdehyde (MDA), total protein, superoxide dismutase (SOD), guaiacol peroxidase (GPX) and ascorbate peroxidase (APX) from both conditions and found to be better in intermittent method over conventional practice with higher productivity., (Copyright © 2019 Elsevier Inc. All rights reserved.)

In the present study, the effects of nitrogen supply (low nitrogen: LN and high nitrogen: HN) on As stress (25 μM) responses of rice seedlings were monitored for 7 d. The mean length of primary, adventitious and lateral roots and number of adventitious and lateral roots were significantly improved in LN+As, while further reduced in HN+As, as compared to As alone treatment at 7 d. The LN+As treatment resulted in significant decline in As (848 μg g -1 DW) than that in As alone treatment (1434 μg g -1 DW) in roots but no significant effect was seen in shoot. In contrast, HN+As treatment showed significant increase in shoot As (6.86 μg g -1 DW) as compared to As alone treatment (3.43 μg g -1 DW). The level of nitrate was increased in roots but declined in shoots in As alone treatment. Surprisingly, no improvement in nitrate level was seen in HN+As as compared to that in As alone treatment in both root and shoot. The expression analysis of nitrate transporters (NRT2;1, NRT2;3a, NRT2;4) showed significant differences in expression patterns in As, LN+As and HN+As treatments. In conclusion, nitrogen supply had profound influences on responses of rice plants to As., (Copyright © 2019 Elsevier B.V. All rights reserved.)

The rapid pace of industrial, agricultural and anthropogenic activities in the 20 th century has resulted in contamination of heavy metals across the globe. Arsenic (As) is a ubiquitous, naturally occurring toxic metalloid, contaminating the soil and water and affecting human health in several countries. Several physicochemical methods exist for the cleanup of As contamination but these are expensive and disastrous to microbes and soil. Plant based remediation approaches are low cost and environmentally safe. Hence, extensive biochemical, molecular and genetic experiments have been conducted to understand plants' responses to As stress and have led to the identification of potential genes. The available knowledge needs to be utilized to either reduce As accumulation in crop plants (rice) or to enhance As levels in shoots of hyperaccumulators (Pteris vittata). Gene manipulation using biotechnological tools can be an effective approach to exploit the potential genes (plasmamembrane and vacuolar transporters, glutathione and phytochelatin biosynthetic enzymes, etc.) playing pivotal roles in uptake, translocation, transformation, complexation, and compartmentalization of As in plants. The transgenic plants with increased tolerance to As and altered (increased/decreased) As accumulation have been developed. The need, however, exists to design plants with altered expression of two or more genes for harmonizing various events (like arsenate reduction, arsenite complexation, sequestration and translocation) so as to achieve desirable reduction (crop plants) or increase (phytoremediator plants) in As content. This review sheds light on transgenic approaches adopted to modulate As levels in plants and proposes future directions to achieve desirable results., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Eichhornia crassipes (water hyacinth), imparts deficiency of soluble arsenic and other toxic metal (loid)s through rhizofiltration and phytoaccumulation. Without proper management strategy, this phytoremediation of metal (loid)s might fail and get reverted back to the environment, contaminating the nearby water bodies. This study, focused on bio-conversion of phytoremediating hyacinths, spiked with 100 times and greater arsenic, lead and cadmium concentrations than the average water contamination, ranging in 58.81 ± 0.394, 16.74 ± 0.367, 12.18 ± 0.153 mg Kg -1 arsenic, 18.95 ± 0.212, 9.53 ± 0.054, 6.83 ± 0.306 mg kg -1 lead and 2.79 ± 0.033, 1.39 ± 0.025, 0.92 ± 0.045 mg kg -1 cadmium, respectively in root, shoot and leaves, proving it's phytoaccumulation capacity. Next, these hyacinths has been used as a source of organic supplement for preparing vermicompost using Eisenia fetida following analysis of total metal content and sequential extraction. Control soil was having 134.69 ± 2.47 mg kg -1 arsenic in compare to 44.6 ± 0.91 mg kg -1 at premature stage of compost to 23.9 ± 1.55 mg kg -1 at mature compost indicating sustainable fate of phytoremediated vermicompost. This vermiremediation of arsenic and other toxic elements, restricted the bioavailability of soil pollutants. Furthermore, processed compost amended as organic fertilizer, growing chickpea, coriander, tomato and chilli plant, resulted in negligible metal(loid)s in treated samples, enhancing also plant's growth and production., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

In the present study, the effect of arsenate (AsV) exposure either alone or in combination with calcium (Ca) was investigated in callus cultures of Brassica juncea (L.) Czern. cv. Pusa Bold grown for a period up to 24 h. The AsV (250 μM) + Ca (10 mM) treatment resulted in a significantly higher level of As (464 μg g(-1) dry weight (DW)) than AsV without Ca (167 μg g(-1) DW) treatment at 24 h. Furthermore, AsV + Ca-treated calli had a higher percent of AsIII (24-47%) than calli subjected to AsV treatment (12-14%). Despite this, AsV + Ca-treated calli did not show any signs of hydrogen peroxide (H(2)O(2)) accumulation or cell death upon in vivo staining, while AsV-exposed calli had increased H(2)O(2), shrinkage of cytoplasmic contents, and cell death. Thus, AsV treatment induced oxidative stress, which in turn elicited a response of antioxidant enzymes and metabolites as compared with control and AsV + Ca treatment. The positive effects of Ca supplementation were also correlated to an increase in thiolic constituents', viz., cysteine, reduced glutathione, and glutathione reductase in AsV + Ca than in AsV treatment. An analysis of selected signaling related genes, e.g., mitogen-activated protein kinases (MAPK3 and MAPK6) and jasmonate ZIM-domain (JAZ3) suggested that AsV and AsV + Ca followed variable pathways to sense and signal the As stress. In AsV-alone treatment, jasmonate signaling was seemingly activated, while MAPK3 was not involved. In contrast, AsV + Ca treatment appeared to specifically inhibit jasmonate signaling and activate MAPK3. In conclusion, Ca supplementation may hold promise for achieving increased As accumulation in plants without compromising their tolerance.

Arsenic (As) is a widespread environmental and food chain contaminant and class I, non-threshold carcinogen. Plants accumulate As due to ionic mimicry that is of importance as a measure of phytoremediation but of concern due to the use of plants in alternative medicine. The present study investigated As accumulation in native plants including some medicinal plants, from three districts [Chinsurah (Hoogly), Porbosthali (Bardhman), and Birnagar (Nadia)] of West Bengal, India, having a history of As pollution. A site-specific response was observed for Specific Arsenic Uptake (SAU; mg kg(-1) dw) in total number of 13 (8 aquatic and 5 terrestrial) collected plants. SAU was higher in aquatic plants (5-60 mg kg(-1) dw) than in terrestrial species (4-19 mg kg(-1) dw). The level of As was lower in medicinal plants (MPs) than in non-medicinal plants, however it was still beyond the WHO permissible limit (1 mg kg(-1) dw). The concentration of other elements (Cu, Zn, Se, and Pb) was found to be within prescribed limits in medicinal plants (MP). Among the aquatic plants, Marsilea showed the highest SAU (avg. 45 mg kg(-1) dw), however, transfer factor (TF) of As was the maximum in Centella asiatica (MP, avg. 1). Among the terrestrial plants, the maximum SAU and TF were demonstrated by Alternanthera ficoidea (avg. 15) and Phyllanthus amarus (MP, avg. 1.27), respectively. In conclusion, the direct use of MP or their by products for humans should not be practiced without proper regulation. In other way, one fern species (Marsilea) and some aquatic plants (Eichhornia crassipes and Cyperus difformis) might be suitable candidates for As phytoremediation of paddy fields.

Sesuvium portulacastrum (L.) L., a facultative halophyte, is considered a suitable candidate for the phytoremediation of metals. An investigation of As accumulation and tolerance was conducted in Sesuvium plants upon exposure to As(V) (100-1000 μM) for 30 d. Plants demonstrated a good growth even after prolonged exposure (30 d) to high As(V) concentrations (1000 μM) and a significant As accumulation (155 μg g⁻¹ dry weight) with a bioaccumulation factor of more than ten at each concentration. The results of shoot and root dry weight, malondialdehyde accumulation, photosynthetic pigments, and total soluble proteins demonstrated that plants did not experience significant toxicity even at 1000 μM As(V) after 30 d. However, metabolites (total non-protein thiols and cysteine) and enzymes (serine acetyltransferase, cysteine synthase and γ-glutamylcysteine synthetase) of thiol metabolism, in general, remained either unaffected or showed slight decline. Hence, plants tolerated high As(V) concentrations without an involvement of thiol metabolism as a major component. Taken together, the results indicate that plants are potential As accumulator and may find application in the re-vegetation of As contaminated sites., (Copyright © 2010 Elsevier Ltd. All rights reserved.)

In the present study, Hydrilla verticillata plants were exposed to arsenate (AsV; 50 microM) and arsenite (AsIII; 5 microM) under variable S supply: deficient (2 microM S, -S), normal (1 mM S, +S) and excess (2 mM S, +HS). Arsenic accumulation (microg g(-1) dw) in +HS plants was about 2-fold higher upon exposure to both AsV (30) and AsIII (50) than that observed in +S (12 & 24) and -S (14 & 26) plants. Despite lower As accumulation, -S plants experienced the maximum oxidative stress owing to an inadequate response of enzymatic and molecular antioxidants and significant decline in total thiols and the ratio of reduced glutathione (GSH) to oxidized glutathione (GSSG). By contrast +HS plants had significant increase in total thiols and an improved redox status, did not demonstrate any negative impact to antioxidants except catalase and hence experienced the least increase in oxidative stress parameters. In conclusion, an increase in S supply to plants may improve their accumulation capacity for As through enhanced tolerance caused by a positive effect on thiol metabolism and antioxidant status of the plants., (Copyright (c) 2009 Elsevier Inc. All rights reserved.)

The exposure of paddy fields to arsenic (As) through groundwater irrigation is a serious concern that may not only lead to As accumulation to unacceptable levels but also interfere with mineral nutrients in rice grains. In the present field study, profiling of the mineral nutrients (iron (Fe), phosphorous, zinc, and selenium (Se)) was done in various rice genotypes with respect to As accumulation. A significant genotypic variation was observed in elemental retention on root Fe plaque and their accumulation in various plant parts including grains, specific As uptake (29-167 mg kg(-1) dw), as well as As transfer factor (4-45%). Grains retained the least level of As (0.7-3%) with inorganic As species being the dominant forms, while organic As species, viz., dimethylarsinic acid and monomethylarsonic acid, were non-detectable. In all tested varieties, the level of Se was low (0.05-0.12 mg kg(-1) dw), whereas that of As was high (0.4-1.68 mg kg(-1) dw), considering their safe/recommended daily intake limits, which may not warrant their human consumption. Hence, their utilization may increase the risk of arsenicosis, when grown in As-contaminated areas.

Arsenic toxicity has become a global concern owing to the ever-increasing contamination of water, soil and crops in many regions of the world. To limit the detrimental impact of arsenic compounds, efficient strategies such as phytoremediation are required. Suitable plants include arsenic hyperaccumulating ferns and aquatic plants that are capable of completing their life cycle in the presence of high levels of arsenic through the concerted action of arsenate reduction to arsenite, arsenite complexation, and vacuolar compartmentalization of complexed or inorganic arsenic. Tolerance can also be conferred by lowering arsenic uptake by suppression of phosphate transport activity, a major pathway for arsenate entry. In many unicellular organisms, arsenic tolerance is based on the active removal of cytosolic arsenite while limiting the uptake of arsenate. Recent molecular studies have revealed many of the gene products involved in these processes, providing the tools to improve crop species and to optimize phytoremediation; however, so far only single genes have been manipulated, which has limited progress. We will discuss recent advances and their potential applications, particularly in the context of multigenic engineering approaches.