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Regional imbalance in development is amongst the most important challenges for China. As the country’s leading economic engines, three major economic circles representing 6.5% of the national land territory contributed to more than 2/5 of national gross domestic product (GDP). This paper focuses on the impact of these three growth poles in China’s regional development, particularly for their embodied energy uses in light of multi-regional input-output analysis. Significantly, the three developed areas generated huge demands for embodied energy, 1237.52 Mtce and 40.1% of the total domestic supply in 2010, but far from self-sufficient. In Yangtze-River-Delta , Pearl-River-Delta and Jing-Jin-Ji , 93.1%, 85.1% and 63.4% of their embodied energy uses respectively can be traced back to the energy-supply provinces such as Shanxi, Inner Mongolia and Shaanxi and other trade partners. Over a half of the national interregional transfers of embodied energy via domestic trade were induced by the three economic circles’ final demand, largely resulted from their large-scale infrastructure construction, upgraded consumption structures, high export dependency and ongoing urbanization process. The results indicate that demand-driven embodied energy is a comprehensive indicator to reflect the real energy use patterns of developed regions or megacities and reveal their industrial positions in domestic and even global supply chains. Understanding the temporal and spatial energy transition along with regional development from consumption-based insights is a vital step toward appropriately targeted policy making for the nationwide energy saving and emission reduction. [ABSTRACT FROM AUTHOR]

Abstract Consumption demands are final drivers for the extraction and allocation of natural resources. This paper investigates demand-driven natural resource requirements and spatial outsourcing within China in 2012 by using the latest multi-regional input-output model. Exergy is adopted as a common metric for natural resources input. The total domestic resource exergy requirements amounted to 125.5 EJ, of which the eastern area contributed the largest share of 44.5%, followed by the western area (23.9%), the central area (23.0%) and the northeastern area (8.6%). Investment was the leading final demand category, accounting for 52.9% (66.4 EJ) of national total embodied resource use (ERU). The total trade volumes of embodied resource were equivalent to 69.6% of the total direct resource input (DRI), mostly transferred from the central and western regions such as Inner Mongolia, Shanxi, Shaanxi and Xinjiang to the eastern regions such as Jiangsu, Zhejiang, Guangdong and Shanghai. The northeastern and eastern areas had physical net imports of 1213.5 PJ and 38452.6 PJ, while the central and western inland areas had physical net exports of 6364.5 PJ and 33301.5 PJ, respectively. Shanghai, Beijing, Zhejiang, Jiangsu and Guangdong had prominent ERUs which respectively were 101.6, 12.6, 11.7, 8.4 and 4.3 times of their DRIs. The ERUs of Inner Mongolia, Shaanxi, Shanxi, Ningxia and Guizhou were equal to only 17.6%, 25.3%, 27.9%, 46.0% and 50.2% of their DRIs, respectively. Regional uneven development resulted in imbalanced resource requirements across China. The findings can provide a deep understanding of China's resource-driven economic development mode, and contribute to reducing regional resource footprints and their environment outcomes under the "new normal economy". Highlights • Outsourcing resource requirements within China 2012 is systematically revealed. • Exergy is adopted as a common metric for various natural resources input. • 69.6% of national total resource requirements were transferred via domestic trade. • Regional resource use patterns under the "new normal economy" are identified. [ABSTRACT FROM AUTHOR]

Primary energy requirements have close interaction with resource, technology, environment, infrastructure, as well as the socio-economic development. This study links the entire supply chain of the Chinese economy from energy extraction to final consumption by using input-output analysis and structural path analysis. The results show that the domestic primary energy input amounted to 3318.7 Mtce in 2012, of which 49.5% was induced by investment demands. Despite being one of the world's largest energy importers, embodied energy uses (EEUs) in China’s exports were equivalent to about one fourth of its total domestic supply. All Manufacturing sectors accounted for 44.3% of the total EEUs, followed by Construction for 33.3%, Services for 11.6% and Power & Heat for 3.9%. After examining the embodied energy paths, critical economic sectors such as Construction of Buildings , Construction Installation Activities , Transport Via Road , Production and Supply of Electricity and Steam and Processing of Steel Rolling Processing , and supply chain routes starting from final uses to resource extraction such as “Capital formation → Construction of Buildings → Production and Supply of Electricity and Steam → Production and Supply of Electricity and Steam → Mining and Washing of Coal ”, were identified as the main contributors to China’s raw coal and other primary energy requirements. Restructuring Chinese economy from manufacturing industries to construction and services with huge economic costs cannot fundamentally conserve energy, owing to their almost identical structures in higher production tiers; more appropriate policies on technology efficiency gains, energy mix improvement, economic structure adjustment and green consumption deserve to be considered in the light of upstream and downstream responsibilities from a systematic viewpoint. [ABSTRACT FROM AUTHOR]

Owing to uneven development and unbalanced resource endowments within China, ensuring reliable energy, water and food supply is a core challenge to regional socio-economic development. This study makes a first attempt to examine and compare demand-driven energy, water and arable land (E-W-L) resource outsourcings within China based on the latest multi-regional input-output model. Results show that interprovincial trade reallocated 73.4%, 33.9% and 38.1% of the national total E-W-L resource inputs in 2012, respectively. Investment was the dominant final demand category for driving energy requirements, while consumption was the leading final demand category for water and arable land requirements. Important provincial regions and critical transmission sectors for the trade of embodied E-W-L resources are identified. Substantial E-W-L resources were transferred from the central and western regions to the eastern regions. Especially, Inner Mongolia was the top interregional net exporter of embodied energy, while Jiangsu topped the net importer list. Regarding virtual water transfer, Xinjiang and Shandong were the biggest interregional net exporters and net importers, respectively, while Heilongjiang and Guangdong stood out in the net trade of embodied arable land. Owing to the impact of interprovincial trade, the resource occupancy levels of the eastern developed area were much higher than those of the northeastern, central and western areas. The imbalances in the levels of socio-economic development amongst provincial regions are mirrored by their patterns of E-W-L uses and related trade transfer. Understanding the synchronal outsourcings of E-W-L resource requirements provides important implications for targeted resource management in Chinese interprovincial supply chains. • Energy, water and arable land resource outsourcings within China are examined. • Consumption-based accounting contributes to revealing resource inequality issue. • Eastern developed regions induce large amounts of resource transfers via trade. • Regional uneven development leads to the unequal access to resources. • Managing interprovincial supply chains can reduce resource footprint. [ABSTRACT FROM AUTHOR]

Background: In situ hybridization (ISH) is a general molecular biological technique used to determine the spatiotemporal expression of genes in many species. In the past few years, numerous ISH protocols have been established in many species. Turnip (Brassica rapa var. rapa) is an important crop in the world, especially in the Plateau area of China, and is a traditional Tibetan medicine. However, ISH protocol in turnip has not been established. Results: We explored and established an optimal workflow for mRNA ISH system for turnip which has been evaluated using BrrCLV3 and BrrWUSa. The optimal methods include: (1) fixation method, (2) protease K pretreatment time, (3) probe length and concentration, (4) washing temperature. We also provide advice on weakening background and improving the efficiency of RNA transcription in vitro. The expression of the BrrCLV3 gene in turnip was detected by the optimized system, and the applicability of the system was confirmed by using BrrWUSa. Conclusions: In this study, we established and optimized the mRNA ISH system for turnip. We explored and found that (1) FAA fixative was the optimized fixation method, (2) 30 min was the optimized protease K pretreatment time, (3) 100 bp, 100 ng/ml probe had good hybridization signal, (4) the optimized washing temperature was 52 °C. It provides a powerful method to locate mRNA in the tissue, which can study the expression and function of turnip's genes. As such, it has considerable advantages in terms of time and cost. [ABSTRACT FROM AUTHOR]