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Abstract The aim of this study was to evaluate the impact of salt stress on morphological, yield, biochemical, and molecular attributes of different barley genotypes. Ten genotypes were cultivated at Fayoum Research Station, El-Fayoum Governorate, Egypt, during two seasons (2020–2021 and 2021–2022), and they were exposed to two different salt concentrations (tap water as a control and 8000 ppm). The results showed that genotypes and salt stress had a significant impact on all morphological and physiological parameters. The morphological parameters (plant height) and yield attributes (spike length, number of grains per spike, and grain yield per plant) of all barley genotypes were significantly decreased under salt stress as compared to control plants. Under salt stress, the total soluble sugars, proline, total phenol, total flavonoid, ascorbic acid, malondialdehyde, hydrogen peroxide, and sodium contents of the shoots of all barley genotypes significantly increased while the potassium content decreased. L1, which is considered a sensitive genotype was more affected by salinity stress than the tolerance genotypes L4, L6, L9, and Giza 138. SDS-PAGE of seed proteins demonstrated high levels of genetic variety with a polymorphism rate of 42.11%. All genotypes evaluated revealed significant variations in the seed protein biochemical markers, with new protein bands appearing and other protein bands disappearing in the protein patterns of genotypes cultivated under various conditions. Two molecular marker techniques (SCoT and ISSR primers) were used in this study. Ten Start Codon Targeted (SCoT) primers exhibited a total of 94 fragments with sizes ranging from 1800 base pairs to 100 base pairs; 29 of them were monomorphic, and 65 bands, with a polymorphism of 62.18%, were polymorphic. These bands contained 21 unique bands (9 positive specific markers and 12 negative specific markers). A total of 54 amplified bands with molecular sizes ranging from 2200 to 200 bp were produced using seven Inter Simple Sequence Repeat (ISSR) primers; 31 of them were monomorphic bands and 23 polymorphic bands had a 40.9% polymorphism. The techniques identified molecular genetic markers associated with salt tolerance in barley crop and successfully marked each genotype with distinct bands. The ten genotypes were sorted into two main groups by the unweighted pair group method of arithmetic averages (UPGMA) cluster analysis based on molecular markers and data at a genetic similarity coefficient level of 0.71.

Barley production is essential in Egypt. In the present study, 15 different six-rowed Egyptian barley cultivars were studied. To differentiate between the different cultivars under study in terms of morphological characteristics and ISSR, molecular characterization reactions were carried out. Moreover, four cultivars (Giza 123, Giza 126, Giza 136, and Giza 138) were selected for further studies using scanning electron microscopy (SEM). Computational analysis of the DNA barcoding sequences of the two plastid markers rbcL and matK was executed, and the results were deposited in the NCBI database. The morphological traits showed low statistical significance among the different cultivars under study via the data collected from two seasons, suggesting that the mean field performance of these Egyptian cultivars may be equal under these conditions. The results showed that the phylogenetic tree was divided into four groups, one of which contained the most closely related genotypes in the genetic distance, including Giza 124, Giza 130, Giza 138, Giza 136, and Giza 137, which converge in the indicative uses of farmers. The seed coat of the studied cultivars was “rugose”. The elevation folding of the rugose pattern ranged from 11 ± 1.73 µm (Giza 126) to 14.67 ± 2.43 µm (Giza 123), suggesting variation in seed quality and its uses in feed and the food industry. According to the similarity matrix of ISSR analysis, the highest similarity value (93%) was recorded between Giza 133 and Giza 132, as well as between Giza 2000 and Giza 126. On the other hand, the lowest similarity value (80%) was recorded between Giza 130 and (Giza 133 and Giza 132), indicating that these cultivars were distantly related. Polymorphism information content (PIC) ranged from 0.26 for the primer ISSR UBC 835 to 0.37 for the primers ISSR UBC 814 and ISSR UBC 840. The current study showed that the matK gene is more mutable than the rbcL gene among the tested cultivars.

Electronic absorption and fluorescence spectra of anthracene-9-carboxylic acid (ANCA) were studied in different homogeneous solvents, binary protic/aprotic solvent mixtures and in heterogeneous solutions of the cationic cetyltrimethyl ammonium bromide (CTAB) micelle. Different chemical species of ANCA were identified spectroscopically in different media. The results are discussed on the basis of a mechanism that involves two equilibria: acid-base equilibrium and monomer-dimer equilibrium. These equilibria were found to be very sensitive to the nature of the medium and the concentration of ANCA.

Faults can seriously damage high-voltage (HV) power systems, particularly if they occur on the long overhead transmission line (OHTL) that connects the nuclear power plant (NPP) to the electrical grid. Finding OHTL problems quickly and accurately is essential for the economy, safety, and dependability of the HV power systems. It is essential to pinpoint the problematic phase to avoid unneeded power outages. Thus, one of the most crucial research challenges is now how to identify, classify, and locate OHTL faults. In this study, transient current with high frequency oscillations that arise immediately after a defect at the sending end is investigated in a single-circuit, single-side fed Egyptian 500-kV HV long OHTL. Asymmetric and symmetric faults and locations are also represented in the Alternative Transients Program-Electro Magnetic Transients Program (ATP/EMTP) simulation model under varying fault resistance and inception angles. The proposed solution in this paper is an Optimized Support Vector Machine (OSVM), whose characteristics are optimized via a mutant particle swarm optimization (MPSO) method to detect 500 kV long OHTL faults. The localizer model is also built for practical applications, including power system noise contaminating fault signals. The findings prove that the suggested approach locates the fault in 0.012 seconds from the start of the event, with a 0.0098 percent average percentage error, and without being impacted by differences in fault distance, fault resistance, noise, or fault inception angle. Additionally, the optimised classifier reaches a 99.85% accuracy rate, enhancing line system dependability and advancing nuclear system development.

This paper presents LQR and GA controllers which applied to control the speed of a DC motor and to maintain the rotation of the motor shaft with particular step response. In the state space, the control strategy is the states feedback and the most used techniques are the LQR. Liner quadratic regulator (LQR) provides an optimal control law for a linear system. It’s a control strategy based on minimizing a quadratic performance index. In despite of the good results obtained from these method, the control design is not a straight forward task due to the trial and error method involved in the definition of weight matrices. In such cases, may be hard tuning the controller parameters in order to obtain the optimal behavior of the system. In this work, it proposes a states feedback technique in which there are no trial and error processes involved and the control design is carried out to fulfill specifications, for minimize overshoot and minimize settling and rising times. The proposed technique is based on the use a genetic algorithms. The obtained results show that is possible to design controllers which fulfill design specifications.