23 results on '"Allert, Stefanie"'
Search Results
2. Secretion of the fungal toxin candidalysin is dependent on conserved precursor peptide sequences
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Müller, Rita, König, Annika, Groth, Sabrina, Zarnowski, Robert, Visser, Corissa, Handrianz, Tom, Maufrais, Corinne, Krüger, Thomas, Himmel, Maximilian, Lee, Sejeong, Priest, Emily L., Yildirim, Deniz, Richardson, Jonathan P., Blango, Matthew G., Bougnoux, Marie-Elisabeth, Kniemeyer, Olaf, d’Enfert, Christophe, Brakhage, Axel A., Andes, David R., Trümper, Verena, Nehls, Christian, Kasper, Lydia, Mogavero, Selene, Gutsmann, Thomas, Naglik, Julian R., Allert, Stefanie, and Hube, Bernhard
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- 2024
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3. Candida albicans translocation through the intestinal epithelial barrier is promoted by fungal zinc acquisition and limited by NFκB-mediated barrier protection.
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Sprague, Jakob L., Schille, Tim B., Allert, Stefanie, Trümper, Verena, Lier, Adrian, Großmann, Peter, Priest, Emily L., Tsavou, Antzela, Panagiotou, Gianni, Naglik, Julian R., Wilson, Duncan, Schäuble, Sascha, Kasper, Lydia, and Hube, Bernhard
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CANDIDA albicans ,INTESTINES ,ZINC ,INTESTINAL infections ,TEMPOROPARIETAL junction ,EPITHELIAL cells ,PEPTIDES - Abstract
The opportunistic fungal pathogen Candida albicans thrives on human mucosal surfaces as a harmless commensal, but frequently causes infections under certain predisposing conditions. Translocation across the intestinal barrier into the bloodstream by intestine-colonizing C. albicans cells serves as the main source of disseminated candidiasis. However, the host and microbial mechanisms behind this process remain unclear. In this study we identified fungal and host factors specifically involved in infection of intestinal epithelial cells (IECs) using dual-RNA sequencing. Our data suggest that host-cell damage mediated by the peptide toxin candidalysin-encoding gene ECE1 facilitates fungal zinc acquisition. This in turn is crucial for the full virulence potential of C. albicans during infection. IECs in turn exhibit a filamentation- and damage-specific response to C. albicans infection, including NFκB, MAPK, and TNF signaling. NFκB activation by IECs limits candidalysin-mediated host-cell damage and mediates maintenance of the intestinal barrier and cell-cell junctions to further restrict fungal translocation. This is the first study to show that candidalysin-mediated damage is necessary for C. albicans nutrient acquisition during infection and to explain how IECs counteract damage and limit fungal translocation via NFκB-mediated maintenance of the intestinal barrier. Author summary: Candida albicans populations colonizing the intestine serve as the main source for systemic infections. Though normally commensal, under certain conditions, C. albicans can translocate across the intestine and into the bloodstream, leading to systemic candidiasis. Here we dissect the fungal and host activities involved in this process. We find that damage to host cells, which supports efficient translocation, is associated with active acquisition of host-cell zinc by C. albicans. At the same time, intestinal epithelial cells foster barrier integrity to limit fungal translocation independently of host damage. [ABSTRACT FROM AUTHOR]
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- 2024
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4. FSP1-specific SMAD2 knockout in renal tubular, endothelial, and interstitial cells reduces fibrosis and epithelial-to-mesenchymal transition in murine STZ-induced diabetic nephropathy
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Loeffler, Ivonne, Liebisch, Marita, Allert, Stefanie, Kunisch, Elke, Kinne, Raimund W., and Wolf, Gunter
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- 2018
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5. From environmental adaptation to host survival: Attributes that mediate pathogenicity of Candida auris.
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Allert, Stefanie, Schulz, Daniela, Kämmer, Philipp, Großmann, Peter, Wolf, Thomas, Schäuble, Sascha, Gianni Panagiotou, Brunke, Sascha, and Hube, Bernhard
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- 2022
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6. Candidalysin Is the Hemolytic Factor of Candida albicans.
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Mogavero, Selene, Höfs, Sarah, Lauer, Alexa N., Müller, Rita, Brunke, Sascha, Allert, Stefanie, Gerwien, Franziska, Groth, Sabrina, Dolk, Edward, Wilson, Duncan, Gutsmann, Thomas, and Hube, Bernhard
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CANDIDA albicans ,PEPTIDES ,PURINERGIC receptors ,IMMUNOGLOBULINS ,ARTIFICIAL membranes ,HEMOLYSIS & hemolysins - Abstract
Candida albicans produces an important virulence factor, the hypha-associated Ece1-derived secreted peptide toxin candidalysin, which is crucial for the establishment of mucosal and systemic infections. C. albicans has also long been known to be hemolytic, yet the hemolytic factor has not been clearly identified. Here, we show that candidalysin is the hemolytic factor of C. albicans. Its hemolytic activity is modulated by fragments of another Ece1 peptide, P7. Hemolysis by candidalysin can be neutralized by the purinergic receptor antagonist pyridoxal-phosphate-6-azophenyl-2′,4′-disulfonic acid (PPADS). PPADS also affects candidalysin's ability to intercalate into synthetic membranes. We also describe the neutralization potential of two anti-candidalysin nanobodies, which are promising candidates for future anti-Candida therapy. This work provides evidence that the historically proposed hemolytic factor of C. albicans is in fact candidalysin and sheds more light on the complex roles of this toxin in C. albicans biology and pathogenicity. [ABSTRACT FROM AUTHOR]
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- 2022
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7. Candidalysin delivery to the invasion pocket is critical for host epithelial damage induced by Candida albicans.
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Mogavero, Selene, Sauer, Frank M., Brunke, Sascha, Allert, Stefanie, Schulz, Daniela, Wisgott, Stephanie, Jablonowski, Nadja, Elshafee, Osama, Krüger, Thomas, Kniemeyer, Olaf, Brakhage, Axel A., Naglik, Julian R., Dolk, Edward, and Hube, Bernhard
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CANDIDA albicans ,IMMUNOGLOBULINS ,PATHOGENIC fungi ,EPITHELIAL cells ,CELL membranes ,SECRETION - Abstract
The human pathogenic fungus Candida albicans is a frequent cause of mucosal infections. Although the ability to transition from the yeast to the hypha morphology is essential for virulence, hypha formation and host cell invasion per se are not sufficient for the induction of epithelial damage. Rather, the hypha‐associated peptide toxin, candidalysin, a product of the Ece1 polyprotein, is the critical damaging factor. While synthetic, exogenously added candidalysin is sufficient to damage epithelial cells, the level of damage does not reach the same level as invading C. albicans hyphae. Therefore, we hypothesized that a combination of fungal attributes is required to deliver candidalysin to the invasion pocket to enable the full damaging potential of C. albicans during infection. Utilising a panel of C. albicans mutants with known virulence defects, we demonstrate that the full damage potential of C. albicans requires the coordinated delivery of candidalysin to the invasion pocket. This process requires appropriate epithelial adhesion, hyphal extension and invasion, high levels of ECE1 transcription, proper Ece1 processing and secretion of candidalysin. To confirm candidalysin delivery, we generated camelid VHHs (nanobodies) specific for candidalysin and demonstrate localization and accumulation of the toxin only in C. albicans‐induced invasion pockets. In summary, a defined combination of virulence attributes and cellular processes is critical for delivering candidalysin to the invasion pocket to enable the full damage potential of C. albicans during mucosal infection. Take Aways: Candidalysin is a peptide toxin secreted by C. albicans causing epithelial damage.Candidalysin delivery to host cell membranes requires specific fungal attributes.Candidalysin accumulates in invasion pockets created by invasive hyphae.Camelid nanobodies enabled visualisation of candidalysin in the invasion pocket. [ABSTRACT FROM AUTHOR]
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- 2021
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8. A variant ECE1 allele contributes to reduced pathogenicity of Candida albicans during vulvovaginal candidiasis.
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Liu, Junyan, Willems, Hubertine M. E., Sansevere, Emily A., Allert, Stefanie, Barker, Katherine S., Lowes, David J., Dixson, Andrew C., Xu, Zhenbo, Miao, Jian, DeJarnette, Christian, Tournu, Helene, Palmer, Glen E., Richardson, Jonathan P., Barrera, Francisco N., Hube, Bernhard, Naglik, Julian R., and Peters, Brian M.
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VULVOVAGINAL candidiasis ,CANDIDA albicans ,PATHOLOGY ,GENETIC variation ,ALLELES - Abstract
Vulvovaginal candidiasis (VVC), caused primarily by the human fungal pathogen Candida albicans, results in significant quality-of-life issues for women worldwide. Candidalysin, a toxin derived from a polypeptide (Ece1p) encoded by the ECE1 gene, plays a crucial role in driving immunopathology at the vaginal mucosa. This study aimed to determine if expression and/or processing of Ece1p differs across C. albicans isolates and whether this partly underlies differential pathogenicity observed clinically. Using a targeted sequencing approach, we determined that isolate 529L harbors a similarly expressed, yet distinct Ece1p isoform variant that encodes for a predicted functional candidalysin; this isoform was conserved amongst a collection of clinical isolates. Expression of the ECE1 open reading frame (ORF) from 529L in an SC5314-derived ece1Δ/Δ strain resulted in significantly reduced vaginopathogenicity as compared to an isogenic control expressing a wild-type (WT) ECE1 allele. However, in vitro challenge of vaginal epithelial cells with synthetic candidalysin demonstrated similar toxigenic activity amongst SC5314 and 529L isoforms. Creation of an isogenic panel of chimeric strains harboring swapped Ece1p peptides or HiBiT tags revealed reduced secretion with the ORF from 529L that was associated with reduced virulence. A genetic survey of 78 clinical isolates demonstrated a conserved pattern between Ece1p P2 and P3 sequences, suggesting that substrate specificity around Kex2p-mediated KR cleavage sites involved in protein processing may contribute to differential pathogenicity amongst clinical isolates. Therefore, we present a new mechanism for attenuation of C. albicans virulence at the ECE1 locus. Author summary: Vulvovaginal candidiasis (VVC), caused primarily by the human fungal pathogen Candida albicans, results in significant quality-of-life issues for women worldwide. Candidalysin, a toxin derived from a polypeptide (Ece1p) encoded by the ECE1 gene, plays a crucial role in driving disease pathology. In this study, we aimed to determine if genetic variation of ECE1 existed across a collection of C. albicans clinical isolates. Using a targeted sequencing approach, we identified a conserved variant candidalysin isoform. We then constructed isogenic chimeric strains harboring swapped Ece1p peptides or HiBiT tags between representative isoforms and showed impaired secretion with the variant that was associated with reduced virulence in vitro and in vivo. We also elucidated a conserved pattern between key sequences near lysine-arginine cleavage sites involved in Ece1p polypeptide processing by the Kex2p enzyme that correlate with predicted and experimental candidalysin secretion. Therefore, we present a novel mechanism for attenuation of C. albicans virulence at the ECE1 locus that may partly underlie differential clinical outcomes and contribute to the balance between commensal and pathogen at the vaginal mucosa. [ABSTRACT FROM AUTHOR]
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- 2021
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9. Fungal biotin homeostasis is essential for immune evasion after macrophage phagocytosis and virulence.
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Sprenger, Marcel, Hartung, Teresa S., Allert, Stefanie, Wisgott, Stephanie, Niemiec, Maria J., Graf, Katja, Jacobsen, Ilse D., Kasper, Lydia, and Hube, Bernhard
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BIOTIN ,PHAGOCYTOSIS ,CANDIDA albicans ,HOMEOSTASIS ,TRANSCRIPTION factors ,FUNGUS-bacterium relationships - Abstract
Biotin is an important cofactor for multiple enzymes in central metabolic processes. While many bacteria and most fungi are able to synthesise biotin de novo, Candida spp. are auxotrophic for this vitamin and thus require efficient uptake systems to facilitate biotin acquisition during infection. Here we show that Candida glabrata and Candida albicans use a largely conserved system for biotin uptake and regulation, consisting of the high‐affinity biotin transporter Vht1 and the transcription factor Vhr1. Both species induce expression of biotin‐metabolic genes upon in vitro biotin depletion and following phagocytosis by macrophages, indicating low biotin levels in the Candida‐containing phagosome. In line with this, we observed reduced intracellular proliferation of both Candida cells pre‐starved of biotin and deletion mutants lacking VHR1 or VHT1 genes. VHT1 was essential for the full virulence of C. albicans during systemic mouse infections, and the lack of VHT1 led to reduced fungal burden in C. glabrata‐infected brains and C. albicans‐infected brains and kidneys. Together, our data suggest a critical role of Vht1‐mediated biotin acquisition for C. glabrata and C. albicans during intracellular growth in macrophages and systemic infections. [ABSTRACT FROM AUTHOR]
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- 2020
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10. FSP1-specific <italic>SMAD2</italic> knockout in renal tubular, endothelial, and interstitial cells reduces fibrosis and epithelial-to-mesenchymal transition in murine STZ-induced diabetic nephropathy.
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Loeffler, Ivonne, Liebisch, Marita, Allert, Stefanie, Kunisch, Elke, Kinne, Raimund W., and Wolf, Gunter
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FIBROSIS ,RENAL tubular transport ,EPITHELIAL cells ,DIABETIC nephropathies ,TRANSFORMING growth factors - Abstract
Extracellular matrix deposition during tubulointerstitial fibrosis (TIF), a central pathological process in patients with diabetic nephropathy (DN), is driven by locally activated, disease-relevant myofibroblasts. Myofibroblasts can arise from various cellular sources, e.g., tubular epithelial cells via a process named epithelial-to-mesenchymal transition (EMT). Transforming growth factor beta 1 (TGF-β1) and its downstream Smad signaling play a critical role in both TIF and EMT. Whereas Smad3 is one central mediator, the role of the other prominently expressed variant, Smad2, is not completely understood. In this study, we sought to analyze the role of renal Smad2 in the development of TIF and EMT during streptozotocin-induced DN by using a fibroblast-specific protein 1 (FSP1)-promotor-driven
SMAD2 knockout mouse model with decreased tubular, endothelial, and interstitial Smad2 expression. In contrast to wild-type diabetic mice, diabeticSMAD2 knockout mice showed the following features: (1) significantly reduced DN and TIF (shown by KIM1 expression; periodic acid Schiff staining; collagen I and III, fibronectin, and connective tissue growth factor deposition); (2) significantly reduced tubular EMT-like changes (e.g., altered Snail1, E-cadherin, matrix metalloproteinase 2, and vimentin deposition); and (3) significantly decreased expression of myofibroblast markers (α-smooth muscle actin, FSP1). As one mechanism for the protection against diabetes-induced TIF and EMT, decreased Smad3 protein levels and, as a possible consequence, reduced TGF-β1 levels were observed in diabeticSMAD2 knockout mice. Our findings thus support the important role of Smad2 for pro-fibrotic TGF-β/Smad3 signaling in experimental DN. [ABSTRACT FROM AUTHOR]- Published
- 2018
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11. The Snf1-activating kinase Sak1 is a key regulator of metabolic adaptation and in vivo fitness of Candida albicans.
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Ramírez‐Zavala, Bernardo, Mottola, Austin, Haubenreißer, Julia, Schneider, Sabrina, Allert, Stefanie, Brunke, Sascha, Ohlsen, Knut, Hube, Bernhard, and Morschhäuser, Joachim
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CANDIDA albicans ,BACTERIAL genetics ,PHENOTYPIC plasticity ,COLONIZATION (Ecology) ,GENE expression in bacteria - Abstract
The metabolic flexibility of the opportunistic fungal pathogen Candida albicans is important for colonisation and infection of different host niches. Complex regulatory networks, in which protein kinases play central roles, link metabolism and other virulence-associated traits, such as filamentous growth and stress resistance, and thereby control commensalism and pathogenicity. By screening a protein kinase deletion mutant library that was generated in the present work using an improved SAT1 flipper cassette, we found that the previously uncharacterised kinase Sak1 is a key upstream activator of the protein kinase Snf1, a highly conserved regulator of nutrient stress responses that is essential for viability in C. albicans. The sak1Δ mutants failed to grow on many alternative carbon sources and were hypersensitive to cell wall/membrane stress. These phenotypes were mirrored in mutants lacking other subunits of the SNF1 complex and partially compensated by a hyperactive form of Snf1. Transcriptional profiling of sak1Δ mutants showed that Sak1 ensures basal expression of glyoxylate cycle and gluconeogenesis genes even in glucose-rich media and thereby contributes to the metabolic plasticity of C. albicans. In a mouse model of gastrointestinal colonisation, sak1Δ mutants were rapidly outcompeted by wild-type cells, demonstrating that Sak1 is essential for the in vivo fitness of C. albicans. [ABSTRACT FROM AUTHOR]
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- 2017
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12. In Vivo Transcriptional Profiling of Human Pathogenic Fungi during Infection: Reflecting the Real Life?
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Allert, Stefanie, Brunke, Sascha, and Hube, Bernhard
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GENE expression profiling , *PATHOGENIC fungi , *INFECTION , *GENE expression , *RNA sequencing - Abstract
The article focuses on the in vivo transcriptional profiling of human pathogenic fungi during infection. Topics include the microbial infections in humans caused by viruses, bacteria, fungi, or parasites, the identification of infections through genomes of the pathogen and host and appropriate gene expression, and the RNA sequencing.
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- 2016
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13. Identification of Candida glabrata Genes Involved in pH Modulation and Modification of the Phagosomal Environment in Macrophages.
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Kasper, Lydia, Seider, Katja, Gerwien, Franziska, Allert, Stefanie, Brunke, Sascha, Schwarzmüller, Tobias, Ames, Lauren, Zubiria-Barrera, Cristina, Mansour, Michael K., Becken, Ulrike, Barz, Dagmar, Vyas, Jatin M., Reiling, Norbert, Haas, Albert, Haynes, Ken, Kuchler, Karl, and Hube, Bernhard
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CANDIDA ,HYDROGEN-ion concentration ,MACROPHAGES ,INVASIVE candidiasis ,ENDOSOMES ,CELL differentiation ,CELLULAR signal transduction - Abstract
Candida glabrata currently ranks as the second most frequent cause of invasive candidiasis. Our previous work has shown that C. glabrata is adapted to intracellular survival in macrophages and replicates within non-acidified late endosomal-stage phagosomes. In contrast, heat killed yeasts are found in acidified matured phagosomes. In the present study, we aimed at elucidating the processes leading to inhibition of phagosome acidification and maturation. We show that phagosomes containing viable C. glabrata cells do not fuse with pre-labeled lysosomes and possess low phagosomal hydrolase activity. Inhibition of acidification occurs independent of macrophage type (human/murine), differentiation (M1-/M2-type) or activation status (vitamin D
3 stimulation). We observed no differential activation of macrophage MAPK or NFκB signaling cascades downstream of pattern recognition receptors after internalization of viable compared to heat killed yeasts, but Syk activation decayed faster in macrophages containing viable yeasts. Thus, delivery of viable yeasts to non-matured phagosomes is likely not triggered by initial recognition events via MAPK or NFκB signaling, but Syk activation may be involved. Although V-ATPase is abundant in C. glabrata phagosomes, the influence of this proton pump on intracellular survival is low since blocking V-ATPase activity with bafilomycin A1 has no influence on fungal viability. Active pH modulation is one possible fungal strategy to change phagosome pH. In fact, C. glabrata is able to alkalinize its extracellular environment, when growing on amino acids as the sole carbon source in vitro. By screening a C. glabrata mutant library we identified genes important for environmental alkalinization that were further tested for their impact on phagosome pH. We found that the lack of fungal mannosyltransferases resulted in severely reduced alkalinization in vitro and in the delivery of C. glabrata to acidified phagosomes. Therefore, protein mannosylation may play a key role in alterations of phagosomal properties caused by C. glabrata. [ABSTRACT FROM AUTHOR]- Published
- 2014
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14. Calcium-dependent ESCRT recruitment and lysosome exocytosis maintain epithelial integrity during Candida albicans invasion.
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Westman, Johannes, Plumb, Jonathan, Licht, Anna, Yang, Mabel, Allert, Stefanie, Naglik, Julian R., Hube, Bernhard, Grinstein, Sergio, and Maxson, Michelle E.
- Abstract
Candida albicans is both a commensal and an opportunistic fungal pathogen. Invading hyphae of C. albicans secrete candidalysin, a pore-forming peptide toxin. To prevent cell death, epithelial cells must protect themselves from direct damage induced by candidalysin and by the mechanical forces exerted by expanding hyphae. We identify two key Ca
2+ -dependent repair mechanisms employed by epithelial cells to withstand candidalysin-producing hyphae. Using camelid nanobodies, we demonstrate candidalysin secretion directly into the invasion pockets induced by elongating C. albicans hyphae. The toxin induces oscillatory increases in cytosolic [Ca2+ ], which cause hydrolysis of PtdIns(4,5)P 2 and loss of cortical actin. Epithelial cells dispose of damaged membrane regions containing candidalysin by an Alg-2/Alix/ESCRT-III-dependent blebbing process. At later stages, plasmalemmal tears induced mechanically by invading hyphae are repaired by exocytic insertion of lysosomal membranes. These two repair mechanisms maintain epithelial integrity and prevent mucosal damage during both commensal growth and infection by C. albicans. [Display omitted] • C. albicans invasion and candidalysin secretion trigger epithelial responses • Candidalysin causes Ca2+ influx, PtdIns(4,5)P 2 hydrolysis, and breakdown of F-actin • ESCRT and exocytosis of lysosomes repair the host membrane and remove candidalysin • Silencing the ESCRT machinery curtails the viability of candidalysin-treated cells The human fungal pathogen Candida albicans invades epithelial cells and secretes the pore-forming toxin candidalysin. Westman et al. demonstrate that epithelial cells implement two membrane repair mechanisms—ESCRT-mediated plasma membrane pinching and lysosome exocytosis—that remove candidalysin and confer resistance to the invading fungus [ABSTRACT FROM AUTHOR]- Published
- 2022
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15. Cover Image: Candidalysin delivery to the invasion pocket is critical for host epithelial damage induced by Candida albicans (Cellular Microbiology 10/2021).
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Mogavero, Selene, Sauer, Frank M., Brunke, Sascha, Allert, Stefanie, Schulz, Daniela, Wisgott, Stephanie, Jablonowski, Nadja, Elshafee, Osama, Krüger, Thomas, Kniemeyer, Olaf, Brakhage, Axel A., Naglik, Julian R., Dolk, Edward, and Hube, Bernhard
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CANDIDA albicans ,MICROBIOLOGY ,EPITHELIAL cells - Published
- 2021
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16. A highly conserved tRNA modification contributes to C. albicans filamentation and virulence.
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Böttcher B, Kienast SD, Leufken J, Eggers C, Sharma P, Leufken CM, Morgner B, Drexler HCA, Schulz D, Allert S, Jacobsen ID, Vylkova S, Leidel SA, and Brunke S
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- Virulence genetics, Humans, Animals, Candida pathogenicity, Candida genetics, Candida metabolism, Host-Pathogen Interactions, Mice, Epithelial Cells microbiology, Candida albicans genetics, Candida albicans pathogenicity, Candida albicans metabolism, RNA, Transfer genetics, RNA, Transfer metabolism, Fungal Proteins genetics, Fungal Proteins metabolism, Candidiasis microbiology, Hyphae growth & development, Hyphae genetics, Hyphae metabolism
- Abstract
tRNA modifications play important roles in maintaining translation accuracy in all domains of life. Disruptions in the tRNA modification machinery, especially of the anticodon stem loop, can be lethal for many bacteria and lead to a broad range of phenotypes in baker's yeast. Very little is known about the function of tRNA modifications in host-pathogen interactions, where rapidly changing environments and stresses require fast adaptations. We found that two closely related fungal pathogens of humans, the highly pathogenic Candida albicans and its much less pathogenic sister species, Candida dubliniensis , differ in the function of a tRNA-modifying enzyme. This enzyme, Hma1, exhibits species-specific effects on the ability of the two fungi to grow in the hypha morphology, which is central to their virulence potential. We show that Hma1 has tRNA-threonylcarbamoyladenosine dehydratase activity, and its deletion alters ribosome occupancy, especially at 37°C-the body temperature of the human host. A C. albicans HMA1 deletion mutant also shows defects in adhesion to and invasion into human epithelial cells and shows reduced virulence in a fungal infection model. This links tRNA modifications to host-induced filamentation and virulence of one of the most important fungal pathogens of humans.IMPORTANCEFungal infections are on the rise worldwide, and their global burden on human life and health is frequently underestimated. Among them, the human commensal and opportunistic pathogen, Candida albicans, is one of the major causative agents of severe infections. Its virulence is closely linked to its ability to change morphologies from yeasts to hyphae. Here, this ability is linked-to our knowledge for the first time-to modifications of tRNA and translational efficiency. One tRNA-modifying enzyme, Hma1, plays a specific role in C. albicans and its ability to invade the host. This adds a so-far unknown layer of regulation to the fungal virulence program and offers new potential therapeutic targets to fight fungal infections., Competing Interests: The authors declare no conflict of interest.
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- 2024
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17. Nanobody-mediated neutralization of candidalysin prevents epithelial damage and inflammatory responses that drive vulvovaginal candidiasis pathogenesis.
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Valentine M, Rudolph P, Dietschmann A, Tsavou A, Mogavero S, Lee S, Priest EL, Zhurgenbayeva G, Jablonowski N, Timme S, Eggeling C, Allert S, Dolk E, Naglik JR, Figge MT, Gresnigt MS, and Hube B
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- Humans, Female, Quality of Life, Candida albicans metabolism, Inflammation, Candidiasis, Vulvovaginal microbiology, Single-Domain Antibodies metabolism, Candidiasis microbiology, Fungal Proteins
- Abstract
Candida albicans can cause mucosal infections in humans. This includes oropharyngeal candidiasis, which is commonly observed in human immunodeficiency virus infected patients, and vulvovaginal candidiasis (VVC), which is the most frequent manifestation of candidiasis. Epithelial cell invasion by C. albicans hyphae is accompanied by the secretion of candidalysin, a peptide toxin that causes epithelial cell cytotoxicity. During vaginal infections, candidalysin-driven tissue damage triggers epithelial signaling pathways, leading to hyperinflammatory responses and immunopathology, a hallmark of VVC. Therefore, we proposed blocking candidalysin activity using nanobodies to reduce epithelial damage and inflammation as a therapeutic strategy for VVC. Anti-candidalysin nanobodies were confirmed to localize around epithelial-invading C. albicans hyphae, even within the invasion pocket where candidalysin is secreted. The nanobodies reduced candidalysin-induced damage to epithelial cells and downstream proinflammatory responses. Accordingly, the nanobodies also decreased neutrophil activation and recruitment. In silico mathematical modeling enabled the quantification of epithelial damage caused by candidalysin under various nanobody dosing strategies. Thus, nanobody-mediated neutralization of candidalysin offers a novel therapeutic approach to block immunopathogenic events during VVC and alleviate symptoms.IMPORTANCEWorldwide, vaginal infections caused by Candida albicans (VVC) annually affect millions of women, with symptoms significantly impacting quality of life. Current treatments are based on anti-fungals and probiotics that target the fungus. However, in some cases, infections are recurrent, called recurrent VVC, which often fails to respond to treatment. Vaginal mucosal tissue damage caused by the C. albicans peptide toxin candidalysin is a key driver in the induction of hyperinflammatory responses that fail to clear the infection and contribute to immunopathology and disease severity. In this pre-clinical evaluation, we show that nanobody-mediated candidalysin neutralization reduces tissue damage and thereby limits inflammation. Implementation of candidalysin-neutralizing nanobodies may prove an attractive strategy to alleviate symptoms in complicated VVC cases., Competing Interests: E.D. is the CEO of Q.V.Q who produced the anti-candidalysin nanobodies.
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- 2024
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18. Survival Strategies of Pathogenic Candida Species in Human Blood Show Independent and Specific Adaptations.
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Kämmer P, McNamara S, Wolf T, Conrad T, Allert S, Gerwien F, Hünniger K, Kurzai O, Guthke R, Hube B, Linde J, and Brunke S
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- Candida classification, Candida immunology, Candidiasis blood, Cytokines immunology, Fungal Proteins immunology, Gene Expression Profiling, Humans, Metabolic Networks and Pathways, Microbial Viability, Phylogeny, Virulence, Adaptation, Physiological, Blood microbiology, Candida pathogenicity, Fungal Proteins genetics
- Abstract
Only four species, Candida albicans , C. glabrata , C. parapsilosis , and C. tropicalis , together account for about 90% of all Candida bloodstream infections and are among the most common causes of invasive fungal infections of humans. However, virulence potential varies among these species, and the phylogenetic tree reveals that their pathogenicity may have emerged several times independently during evolution. We therefore tested these four species in a human whole-blood infection model to determine, via comprehensive dual-species RNA-sequencing analyses, which fungal infection strategies are conserved and which are recent evolutionary developments. The ex vivo infection progressed from initial immune cell interactions to nearly complete killing of all fungal cells. During the course of infection, we characterized important parameters of pathogen-host interactions, such as fungal survival, types of interacting immune cells, and cytokine release. On the transcriptional level, we obtained a predominantly uniform and species-independent human response governed by a strong upregulation of proinflammatory processes, which was downregulated at later time points after most of the fungal cells were killed. In stark contrast, we observed that the different fungal species pursued predominantly individual strategies and showed significantly different global transcriptome patterns. Among other findings, our functional analyses revealed that the fungal species relied on different metabolic pathways and virulence factors to survive the host-imposed stress. These data show that adaptation of Candida species as a response to the host is not a phylogenetic trait, but rather has likely evolved independently as a prerequisite to cause human infections. IMPORTANCE To ensure their survival, pathogens have to adapt immediately to new environments in their hosts, for example, during the transition from the gut to the bloodstream. Here, we investigated the basis of this adaptation in a group of fungal species which are among the most common causes of hospital-acquired infections, the Candida species. On the basis of a human whole-blood infection model, we studied which genes and processes are active over the course of an infection in both the host and four different Candida pathogens. Remarkably, we found that, while the human host response during the early phase of infection is predominantly uniform, the pathogens pursue largely individual strategies and each one regulates genes involved in largely disparate processes in the blood. Our results reveal that C. albicans , C. glabrata , C. parapsilosis , and C. tropicalis all have developed individual strategies for survival in the host. This indicates that their pathogenicity in humans has evolved several times independently and that genes which are central for survival in the host for one species may be irrelevant in another., (Copyright © 2020 Kämmer et al.)
- Published
- 2020
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19. Keeping Candida commensal: how lactobacilli antagonize pathogenicity of Candida albicans in an in vitro gut model.
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Graf K, Last A, Gratz R, Allert S, Linde S, Westermann M, Gröger M, Mosig AS, Gresnigt MS, and Hube B
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- Adhesiveness, Apoptosis, Caco-2 Cells, Cell Aggregation, Enterocytes microbiology, Enterocytes pathology, Enterocytes ultrastructure, Epithelium microbiology, Epithelium pathology, Gene Expression Regulation, Glucose metabolism, Host-Pathogen Interactions, Humans, Hyphae metabolism, Lactates metabolism, Lactobacillus growth & development, Microbial Viability, Oxygen metabolism, Polysaccharides, Bacterial metabolism, Species Specificity, Stress, Physiological, Time Factors, Candida albicans physiology, Gastrointestinal Tract microbiology, Lactobacillus physiology, Models, Biological
- Abstract
The intestine is the primary reservoir of Candida albicans that can cause systemic infections in immunocompromised patients. In this reservoir, the fungus exists as a harmless commensal. However, antibiotic treatment can disturb the bacterial microbiota, facilitating fungal overgrowth and favoring pathogenicity. The current in vitro gut models that are used to study the pathogenesis of C. albicans investigate the state in which C. albicans behaves as a pathogen rather than as a commensal. We present a novel in vitro gut model in which the fungal pathogenicity is reduced to a minimum by increasing the biological complexity. In this model, enterocytes represent the epithelial barrier and goblet cells limit C. albicans adhesion and invasion. Significant protection against C. albicans- induced necrotic damage was achieved by the introduction of a microbiota of antagonistic lactobacilli. We demonstrated a time-, dose- and species-dependent protective effect against C. albicans -induced cytotoxicity. This required bacterial growth, which relied on the presence of host cells, but was not dependent on the competition for adhesion sites. Lactobacillus rhamnosus reduced hyphal elongation, a key virulence attribute. Furthermore, bacterial-driven shedding of hyphae from the epithelial surface, associated with apoptotic epithelial cells, was identified as a main and novel mechanism of damage protection. However, host cell apoptosis was not the driving mechanism behind shedding. Collectively, we established an in vitro gut model that can be used to experimentally dissect commensal-like interactions of C. albicans with a bacterial microbiota and the host epithelial barrier. We also discovered fungal shedding as a novel mechanism by which bacteria contribute to the protection of epithelial surfaces.This article has an associated First Person interview with the joint first authors of the paper., Competing Interests: Competing interestsThe authors declare no competing or financial interests., (© 2019. Published by The Company of Biologists Ltd.)
- Published
- 2019
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20. Candida albicans-Induced Epithelial Damage Mediates Translocation through Intestinal Barriers.
- Author
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Allert S, Förster TM, Svensson CM, Richardson JP, Pawlik T, Hebecker B, Rudolphi S, Juraschitz M, Schaller M, Blagojevic M, Morschhäuser J, Figge MT, Jacobsen ID, Naglik JR, Kasper L, Mogavero S, and Hube B
- Subjects
- Apoptosis, Candida albicans genetics, Candidiasis physiopathology, Enterocytes cytology, Enterocytes microbiology, Epithelial Cells cytology, Host-Pathogen Interactions, Humans, Intestinal Mucosa cytology, Intestines cytology, Candida albicans physiology, Candidiasis microbiology, Epithelial Cells microbiology, Intestinal Mucosa microbiology, Intestines microbiology
- Abstract
Life-threatening systemic infections often occur due to the translocation of pathogens across the gut barrier and into the bloodstream. While the microbial and host mechanisms permitting bacterial gut translocation are well characterized, these mechanisms are still unclear for fungal pathogens such as Candida albicans , a leading cause of nosocomial fungal bloodstream infections. In this study, we dissected the cellular mechanisms of translocation of C. albicans across intestinal epithelia in vitro and identified fungal genes associated with this process. We show that fungal translocation is a dynamic process initiated by invasion and followed by cellular damage and loss of epithelial integrity. A screen of >2,000 C. albicans deletion mutants identified genes required for cellular damage of and translocation across enterocytes. Correlation analysis suggests that hypha formation, barrier damage above a minimum threshold level, and a decreased epithelial integrity are required for efficient fungal translocation. Translocation occurs predominantly via a transcellular route, which is associated with fungus-induced necrotic epithelial damage, but not apoptotic cell death. The cytolytic peptide toxin of C. albicans , candidalysin, was found to be essential for damage of enterocytes and was a key factor in subsequent fungal translocation, suggesting that transcellular translocation of C. albicans through intestinal layers is mediated by candidalysin. However, fungal invasion and low-level translocation can also occur via non-transcellular routes in a candidalysin-independent manner. This is the first study showing translocation of a human-pathogenic fungus across the intestinal barrier being mediated by a peptide toxin. IMPORTANCE Candida albicans , usually a harmless fungus colonizing human mucosae, can cause lethal bloodstream infections when it manages to translocate across the intestinal epithelium. This can result from antibiotic treatment, immune dysfunction, or intestinal damage (e.g., during surgery). However, fungal processes may also contribute. In this study, we investigated the translocation process of C. albicans using in vitro cell culture models. Translocation occurs as a stepwise process starting with invasion, followed by epithelial damage and loss of epithelial integrity. The ability to secrete candidalysin, a peptide toxin deriving from the hyphal protein Ece1, is key: C. albicans hyphae, secreting candidalysin, take advantage of a necrotic weakened epithelium to translocate through the intestinal layer., (Copyright © 2018 Allert et al.)
- Published
- 2018
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21. Identification of Candida glabrata genes involved in pH modulation and modification of the phagosomal environment in macrophages.
- Author
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Kasper L, Seider K, Gerwien F, Allert S, Brunke S, Schwarzmüller T, Ames L, Zubiria-Barrera C, Mansour MK, Becken U, Barz D, Vyas JM, Reiling N, Haas A, Haynes K, Kuchler K, and Hube B
- Subjects
- Animals, Candidiasis metabolism, Cell Differentiation immunology, Cell Line, Humans, Hydrogen-Ion Concentration, Intracellular Space immunology, Intracellular Space metabolism, Intracellular Space microbiology, Lysosomes immunology, Lysosomes microbiology, Macrophage Activation immunology, Macrophages cytology, Macrophages metabolism, Macrophages microbiology, Mice, Phagosomes metabolism, Phagosomes microbiology, Signal Transduction, Candida glabrata genetics, Candida glabrata immunology, Candidiasis immunology, Candidiasis microbiology, Macrophages immunology, Phagosomes immunology
- Abstract
Candida glabrata currently ranks as the second most frequent cause of invasive candidiasis. Our previous work has shown that C. glabrata is adapted to intracellular survival in macrophages and replicates within non-acidified late endosomal-stage phagosomes. In contrast, heat killed yeasts are found in acidified matured phagosomes. In the present study, we aimed at elucidating the processes leading to inhibition of phagosome acidification and maturation. We show that phagosomes containing viable C. glabrata cells do not fuse with pre-labeled lysosomes and possess low phagosomal hydrolase activity. Inhibition of acidification occurs independent of macrophage type (human/murine), differentiation (M1-/M2-type) or activation status (vitamin D3 stimulation). We observed no differential activation of macrophage MAPK or NFκB signaling cascades downstream of pattern recognition receptors after internalization of viable compared to heat killed yeasts, but Syk activation decayed faster in macrophages containing viable yeasts. Thus, delivery of viable yeasts to non-matured phagosomes is likely not triggered by initial recognition events via MAPK or NFκB signaling, but Syk activation may be involved. Although V-ATPase is abundant in C. glabrata phagosomes, the influence of this proton pump on intracellular survival is low since blocking V-ATPase activity with bafilomycin A1 has no influence on fungal viability. Active pH modulation is one possible fungal strategy to change phagosome pH. In fact, C. glabrata is able to alkalinize its extracellular environment, when growing on amino acids as the sole carbon source in vitro. By screening a C. glabrata mutant library we identified genes important for environmental alkalinization that were further tested for their impact on phagosome pH. We found that the lack of fungal mannosyltransferases resulted in severely reduced alkalinization in vitro and in the delivery of C. glabrata to acidified phagosomes. Therefore, protein mannosylation may play a key role in alterations of phagosomal properties caused by C. glabrata.
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- 2014
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22. Immune evasion, stress resistance, and efficient nutrient acquisition are crucial for intracellular survival of Candida glabrata within macrophages.
- Author
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Seider K, Gerwien F, Kasper L, Allert S, Brunke S, Jablonowski N, Schwarzmüller T, Barz D, Rupp S, Kuchler K, and Hube B
- Subjects
- Calcium metabolism, Candida glabrata genetics, Candida glabrata metabolism, Cell Line, Cell Wall genetics, Cell Wall metabolism, Fungal Proteins genetics, Gene Deletion, Humans, Iron metabolism, Macrophages immunology, Macrophages metabolism, Neutrophils immunology, Neutrophils microbiology, Reactive Oxygen Species metabolism, Tumor Necrosis Factor-alpha genetics, Tumor Necrosis Factor-alpha metabolism, Virulence genetics, Candida glabrata pathogenicity, Fungal Proteins metabolism, Genome, Fungal, Macrophages microbiology, Oxidative Stress, Phagocytosis
- Abstract
Candida glabrata is both a human fungal commensal and an opportunistic pathogen which can withstand activities of the immune system. For example, C. glabrata can survive phagocytosis and replicates within macrophages. However, the mechanisms underlying intracellular survival remain unclear. In this work, we used a functional genomic approach to identify C. glabrata determinants necessary for survival within human monocyte-derived macrophages by screening a set of 433 deletion mutants. We identified 23 genes which are required to resist killing by macrophages. Based on homologies to Saccharomyces cerevisiae orthologs, these genes are putatively involved in cell wall biosynthesis, calcium homeostasis, nutritional and stress response, protein glycosylation, or iron homeostasis. Mutants were further characterized using a series of in vitro assays to elucidate the genes' functions in survival. We investigated different parameters of C. glabrata-phagocyte interactions: uptake by macrophages, replication within macrophages, phagosomal pH, and recognition of mutant cells by macrophages as indicated by production of reactive oxygen species and tumor necrosis factor alpha (TNF-α). We further studied the cell surface integrity of mutant cells, their ability to grow under nutrient-limited conditions, and their susceptibility to stress conditions mirroring the harsh environment inside a phagosome. Additionally, resistance to killing by neutrophils was analyzed. Our data support the view that immune evasion is a key aspect of C. glabrata virulence and that increased immune recognition causes increased antifungal activities by macrophages. Furthermore, stress resistance and efficient nutrient acquisition, in particular, iron uptake, are crucial for intraphagosomal survival of C. glabrata.
- Published
- 2014
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23. Clotrimazole dampens vaginal inflammation and neutrophil infiltration in response to Candida albicans infection.
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Wilson D, Hebecker B, Moyes DL, Miramón P, Jablonowski N, Wisgott S, Allert S, Naglik JR, and Hube B
- Subjects
- Candida albicans drug effects, Candida albicans pathogenicity, Candidiasis, Vulvovaginal immunology, Cell Line, Female, Humans, Candidiasis, Vulvovaginal drug therapy, Clotrimazole therapeutic use, Neutrophil Infiltration drug effects
- Abstract
The pathology of vulvovaginal candidiasis (VVC) caused by Candida albicans is associated with a nonprotective inflammatory response and is frequently treated with clotrimazole. We investigated the mechanisms by which clotrimazole resolves VVC. Low levels of clotrimazole, which do not block fungal growth, inhibit expression of a "danger response" transcription factor, c-Fos, block production of proinflammatory cytokines, and inhibit neutrophil infiltration to the site of infection.
- Published
- 2013
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