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1. Multi-locus sequence analysis reveals great genetic diversity among Mycoplasma capricolum subsp. capripneumoniae strains in Asia

Author

Arooba Akhtar, Anne Boissière, Huafang Hao, Muhammad Saeed, Virginie Dupuy, Antoni Exbrayat, Farhan Anwar Khan, Yuefeng Chu, and Lucía Manso-Silván

Subjects
Mycoplasma capricolum subsp. capripneumoniae, contagious caprine pleuropneumonia, multi-locus sequence analysis, molecular epidemiology, Asia, Africa, Veterinary medicine, SF600-1100
Abstract

Abstract Multi-Locus Sequence Analysis (MLSA) of Mycoplasma capricolum subsp. capripneumoniae (Mccp) strains from Asia revealed unforeseen diversity and a central position for genotyping groups representing strains from Central/East Asia, suggesting a possible origin of contagious caprine pleuropneumonia in this continent. A better assessment of the emergence, diversity and distribution of Mccp in Asia and Africa calls for renewed efforts to dramatically enlarge the sample of strains. Availability and affordability in the field, added to superior typeability (directly from poor samples) and high stability, discriminatory power and concordance with epidemiological and phylogenetic analyses, make MLSA an excellent tool for such investigations.

Published
2022
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2. Mouvement vivant et architecture: la marche, les escaliers chez Émile Jaques-Dalcroze et Adolphe Appia

Author

Anne Boissière

Subjects
dance, butoh, performance, sublime, Philosophy (General), B1-5802, Ethics, BJ1-1725
Abstract

At the turn of the nineteenth and twentieth centuries, through the eurhythmics of Émile Jaques-Dalcroze, a new relationship between music and movement was established, which closely involved dance and architecture. Our emphasis, here, will be put on the transformations related to space, with regard to the stage reform by Adolphe Appia, Dalcroze’s friend and collaborator. It is through Appia’s drawings, the Rhythmic Spaces, made between 1909 and 1911, that the connection between living movement and architecture will be approached. In particular, one calls in question the status of straight lines, horizontal and vertical, of which the staircases, on the drawings, are emblematic. These lines, contrasting with the curving and serpentines lines that mark the influence of the dancing movement in the paintings from that period, must be understood with respect to the Dalcrozian conception of rhythmic feeling. Aliveness, for him, which is not to be separated from the relationship to measure - itself alive -, can be acquired by varied and complex exercises of marching within the framework of rhythmic education. Through architecture arises a musical conception of space in rupture with the perspectival conception of pictural space, as the former doesn’t dissociate any more mobility and immobility, aliveness and inertia, grace and gravity.

Published
2023
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3. Cell-free and intracellular nucleic acids: new non-invasive biomarkers to explore male infertility

Author

Anne Boissière, Anna Gala, Alice Ferrières-Hoa, Tiffany Mullet, Solenne Baillet, Amaël Petiton, Antoine Torre, and Samir Hamamah

Subjects
cf-DNA, cf-mRNA, miRNA, piRNA, Male infertility, Seminal plasma, Medicine (General), R5-920
Abstract

Résumé L’infertilité masculine est un problème qui touche de nombreux couples. Cependant, aujourd’hui les mécanismes moléculaires et les causes de l’infertilité masculine idiopathique ne sont pas élucidés. Les acides nucléiques circulant ont un rôle important dans la physiologie et des évidences suggèrent qu’ils jouent un rôle dans l’infertilité masculine. L’objectif de cette revue est de mettre en avant les nouvelles avancées scientifiques sur les acides nucléiques circulant et non-circulant en lien avec l’infertilité masculine et de fournir une vue d’ensemble de leurs utilisation comme biomarqueurs en pratique clinique.

Published
2017
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6. Música, gesto y espacio en Th. W. Adorno

Author

Anne BOISSIÈRE

Subjects
gesto, espacio, música, ritmo, Philosophy (General), B1-5802
Abstract

Presentamos algunas ideas centrales de la filosofía de la música de Theodor W. Adorno en relación con el aspecto corporal de la música: las relativas al espacio, al gesto y al ritmo.

Published
2011

7. Modulation of malaria infection in Anopheles gambiae mosquitoes exposed to natural midgut bacteria.

Author

Majoline T Tchioffo, Anne Boissière, Thomas S Churcher, Luc Abate, Geoffrey Gimonneau, Sandrine E Nsango, Parfait H Awono-Ambéné, Richard Christen, Antoine Berry, and Isabelle Morlais

Subjects
Medicine, Science
Abstract

The development of Plasmodium falciparum within the Anopheles gambiae mosquito relies on complex vector-parasite interactions, however the resident midgut microbiota also plays an important role in mediating parasite infection. In natural conditions, the mosquito microbial flora is diverse, composed of commensal and symbiotic bacteria. We report here the isolation of culturable midgut bacteria from mosquitoes collected in the field in Cameroon and their identification based on the 16S rRNA gene sequencing. We next measured the effect of selected natural bacterial isolates on Plasmodium falciparum infection prevalence and intensity over multiple infectious feedings and found that the bacteria significantly reduced the prevalence and intensity of infection. These results contrast with our previous study where the abundance of Enterobacteriaceae positively correlated with P. falciparum infection (Boissière et al. 2012). The oral infection of bacteria probably led to the disruption of the gut homeostasis and activated immune responses, and this pinpoints the importance of studying microbe-parasite interactions in natural conditions. Our results indicate that the effect of bacterial exposure on P. falciparum infection varies with factors from the parasite and the human host and calls for deeper dissection of these parameters for accurate interpretation of bacterial exposure results in laboratory settings.

Published
2013
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9. Application of a qPCR assay in the investigation of susceptibility to malaria infection of the M and S molecular forms of An. gambiae s.s. in Cameroon.

Author

Anne Boissière, Geoffrey Gimonneau, Majoline T Tchioffo, Luc Abate, Albert Bayibeki, Parfait H Awono-Ambéné, Sandrine E Nsango, and Isabelle Morlais

Subjects
Medicine, Science
Abstract

Plasmodium falciparum is the causative agent of malaria, a disease that kills almost one million persons each year, mainly in sub-Saharan Africa. P. falciparum is transmitted to the human host by the bite of an Anopheles female mosquito, and Anopheles gambiae sensus stricto is the most tremendous malaria vector in Africa, widespread throughout the afro-tropical belt. An. gambiae s.s. is subdivided into two distinct molecular forms, namely M and S forms. The two molecular forms are morphologically identical but they are distinct genetically, and differ by their distribution and their ecological preferences. The epidemiological importance of the two molecular forms in malaria transmission has been poorly investigated so far and gave distinct results in different areas. We have developed a real-time quantitative PCR (qPCR) assay, and used it to detect P. falciparum at the oocyst stage in wild An. gambiae s.s. mosquitoes experimentally infected with natural isolates of parasites. Mosquitoes were collected at immature stages in sympatric and allopatric breeding sites and further infected at the adult stage. We next measured the infection prevalence and intensity in female mosquitoes using the qPCR assay and correlated the infection success with the mosquito molecular forms. Our results revealed different prevalence of infection between the M and S molecular forms of An. gambiae s.s. in Cameroon, for both sympatric and allopatric populations of mosquitoes. However, no difference in the infection intensity was observed. Thus, the distribution of the molecular forms of An. gambiae s.s. may impact on the malaria epidemiology, and it will be important to monitor the efficiency of malaria control interventions on the two M and S forms.

Published
2013
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10. Midgut microbiota of the malaria mosquito vector Anopheles gambiae and interactions with Plasmodium falciparum infection.

Author

Anne Boissière, Majoline T Tchioffo, Dipankar Bachar, Luc Abate, Alexandra Marie, Sandrine E Nsango, Hamid R Shahbazkia, Parfait H Awono-Ambene, Elena A Levashina, Richard Christen, and Isabelle Morlais

Subjects
Immunologic diseases. Allergy, RC581-607, Biology (General), QH301-705.5
Abstract

The susceptibility of Anopheles mosquitoes to Plasmodium infections relies on complex interactions between the insect vector and the malaria parasite. A number of studies have shown that the mosquito innate immune responses play an important role in controlling the malaria infection and that the strength of parasite clearance is under genetic control, but little is known about the influence of environmental factors on the transmission success. We present here evidence that the composition of the vector gut microbiota is one of the major components that determine the outcome of mosquito infections. A. gambiae mosquitoes collected in natural breeding sites from Cameroon were experimentally challenged with a wild P. falciparum isolate, and their gut bacterial content was submitted for pyrosequencing analysis. The meta-taxogenomic approach revealed a broader richness of the midgut bacterial flora than previously described. Unexpectedly, the majority of bacterial species were found in only a small proportion of mosquitoes, and only 20 genera were shared by 80% of individuals. We show that observed differences in gut bacterial flora of adult mosquitoes is a result of breeding in distinct sites, suggesting that the native aquatic source where larvae were grown determines the composition of the midgut microbiota. Importantly, the abundance of Enterobacteriaceae in the mosquito midgut correlates significantly with the Plasmodium infection status. This striking relationship highlights the role of natural gut environment in parasite transmission. Deciphering microbe-pathogen interactions offers new perspectives to control disease transmission.

Published
2012
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11. Using hematophagous fly blood meals to study the diversity of blood-borne pathogens infecting wild mammals

Author

Solomon Mwakasungula, Virginie Rougeron, Céline Arnathau, Larson Boundenga, Eve Miguel, Anne Boissière, Davy Jiolle, Patrick Durand, Alphonce Msigwa, Sarah Mswata, Ally Olotu, Yvon Sterkers, Benjamin Roche, Gerard Killeen, Frédérique Cerqueira, Paul Yannick Bitome‐Essono, François Bretagnolle, Honorati Masanja, Christophe Paupy, Robert Sumaye, Franck Prugnolle, Ifakara Health Institute [Dar-es-Salaam, Tanzania], Nelson Mandela University [Port Elizabeth], Health, Emergence, Adaptation and Transmission (MIVEGEC-HEAT), Processus Écologiques et Évolutifs au sein des Communautés (PEEC), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM), Centre International de Recherches Médicales de Franceville (CIRMF), Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM), Tanzania National Parks, CHU Montpellier, Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Universidad Nacional Autónoma de México = National Autonomous University of Mexico (UNAM), University College Cork (UCC), Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Centre national de la recherche scientifique et technologique (CENAREST), Biogéosciences [UMR 6282] (BGS), Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Agence Nationale de la Recherche, Grant/Award Number: ANR--10--LABX--04--01, Agence Nationale de la Recherche, Grant/Award Number: ANR--11-LABX--0024, Horizon 2020 Framework Programme, Grant/Award Number: ZIKAlliance(no734548), and ANR-11-LABX-0024,ParaFrap,Alliance française contre les maladies parasitaires(2011)

Subjects
Mammals, Swine, Diptera, invertebrate-derived DNA (iDNA), [SDV]Life Sciences [q-bio], Tsetse flies, blood meals, Animals, Wild, tsetse flies, Invertebrate-derived DNA (iDNA), African Swine Fever Virus, Blood meals, Viruses, wildlife infectious diseases, xenosurveillance, Blood-Borne Pathogens, Genetics, Wildlife infectious diseases, Animals, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Xenosurveillance, Meals, Ecology, Evolution, Behavior and Systematics, Biotechnology
Abstract

International audience; Many emerging infectious diseases originate from wild animals, so there is a profound need for surveillance and monitoring of their pathogens. However, the practical difficulty of sample acquisition from wild animals tends to limit the feasibility and effectiveness of such surveys. Xenosurveillance, using blood-feeding invertebrates to obtain tissue samples from wild animals and then detect their pathogens, is a promising method to do so. Here, we describe the use of tsetse fly blood meals to determine (directly through molecular diagnostic and indirectly through serology), the diversity of circulating blood-borne pathogens (including bacteria, viruses and protozoa) in a natural mammalian community of Tanzania. Molecular analyses of captured tsetse flies (182 pools of flies totalizing 1728 flies) revealed that the blood meals obtained came from 18 different vertebrate species including 16 non-human mammals, representing approximately 25% of the large mammal species present in the study area. Molecular diagnostic demonstrated the presence of different protozoa parasites and bacteria of medical and/or veterinary interest. None of the six virus species searched for by molecular methods were detected but an ELISA test detected antibodies against African swine fever virus among warthogs, indicating that the virus had been circulating in the area. Sampling of blood-feeding insects represents an efficient and practical approach to tracking a diversity of pathogens from multiple mammalian species, directly through molecular diagnostic or indirectly through serology, which could readily expand and enhance our understanding of the ecology and evolution of infectious agents and their interactions with their hosts in wild animal communities.

Published
2022
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12. Lower mgpB diversity in macrolide-resistant Mycoplasma genitalium infecting men visiting two sexually transmitted infection clinics in Montpellier, France

Author

Manon Lounnas, Jennifer Guiraud, Anne-Laure Bañuls, Anne Boissière, Sabine Pereyre, Eric Elguero, Sylvain Godreuil, Chloé Le Roy, Cécile Bébéar, Institut de Recherche pour le Développement (IRD), Laboratoire Mixte International Drug Resistance in Southeast Asia (LMI DRISA), Institut Pasteur du Cambodge, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Oxford University Clinical Research Unit [Ho Chi Minh City] (OUCRU)-Fondation Mérieux-University of sciences and technologies of hanoi (USTH)-Center of Infectiology Lao-Christophe Mérieux [Vientiane] (CILM), Centre de Recherche en Ecologie et Evolution de la Santé (CREES), USC IHMC - Infections humaines à mycoplasmes et à chlamydiae, Institut National de la Recherche Agronomique (INRA), Du gène à l'écosystème (MIVEGEC-GeneSys), Pathogènes, Environnement, Santé Humaine (EPATH), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), and Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])

Subjects
0301 basic medicine, Microbiology (medical), Male, [SDV]Life Sciences [q-bio], 030106 microbiology, Sexually Transmitted Diseases, Single-nucleotide polymorphism, Mycoplasma genitalium, Men who have sex with men, 03 medical and health sciences, Pre-exposure prophylaxis, Sexual and Gender Minorities, 0302 clinical medicine, Antibiotic resistance, Drug Resistance, Bacterial, Prevalence, Medicine, Humans, Pharmacology (medical), Mycoplasma Infections, 030212 general & internal medicine, Typing, Homosexuality, Male, ComputingMilieux_MISCELLANEOUS, Pharmacology, biology, Molecular epidemiology, Transmission (medicine), business.industry, biology.organism_classification, Virology, 3. Good health, Anti-Bacterial Agents, Infectious Diseases, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Female, France, Macrolides, business
Abstract

Objectives Men engaged in high-risk sexual behaviour, such as MSM, are likely to be infected by resistant Mycoplasma genitalium strains. Understanding the transmission dynamics is challenging. We aimed to investigate the molecular epidemiology of M. genitalium in men visiting sexually transmitted infection (STI) clinics. Patients and methods Between June 2017 and February 2018, 95 M. genitalium-positive specimens from 78 men, including 76.9% MSM, visiting two STI clinics in Montpellier, France, were analysed for SNPs in the mgpB adhesin gene and number of tandem repeats in the MG_309 gene. Macrolide and fluoroquinolone resistance were determined. Typing results were compared with antibiotic resistance, sexual behaviour, sampling site, HIV pre-exposure prophylaxis (PrEP) usage and HIV status. Results Thirty-eight mgpB STs were identified, including 23 new STs, with ST4 being most prevalent. The mgpB/MG_309 typing method identified 52 genetic profiles, resulting in a discriminatory index of 0.979. Macrolide and fluoroquinolone resistance-associated mutations were detected in 58.3% and 10.8% of patients, respectively. The macrolide resistance rate was higher among MSM than among men who have sex with women only (68.4% versus 9.1%; adjusted OR, 1.57; 95% CI, 1.13–2.18; P = 0.007). A lower mgpB diversity of 0.870 was found among macrolide-resistant strains in comparison with 0.978 in macrolide-susceptible strains, with an over-representation of mgpB ST62 and ST153. Conclusions Although macrolide resistance spread appears polyclonal in M. genitalium, the lower diversity of mgpB types among macrolide-resistant strains may reflect the easier spread of a few specific mgpB types or the occurrence of sexual networks among MSM.

Published
2020
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13. Population genomic evidence of a Southeast Asian origin of Plasmodium vivax

Author

Patrick Durant, Anne Boissière, Larson Boundenga, Céline Arnathau, Sandrine Houzé, Christine Sidobre, Virginie Rougeron, François Renaud, Michael C. Fontaine, Franck Prugnolle, Josquin Daron, Barthélémy Ngoubangoye, and Jean-François Trape

Subjects
0303 health sciences, education.field_of_study, biology, 030231 tropical medicine, Plasmodium vivax, Population, biology.organism_classification, Southeast asian, medicine.disease, 3. Good health, 03 medical and health sciences, 0302 clinical medicine, Genetic marker, Evolutionary biology, Genetic variation, parasitic diseases, medicine, Clade, education, Malaria, 030304 developmental biology, Founder effect
Abstract

Plasmodium vivaxis the most prevalent and widespread human malaria parasite, with almost three billion people living at risk of infection. With the discovery of its closest genetic relatives in African great apes (Plasmodium vivax-like), the origin ofP. vivaxhas been proposed to be located in the sub-Saharan African area. However, the limited number of genetic markers and samples investigated questioned the robustness of this result. Here, we examined the population genomic variation of 447 humanP. vivaxstrains and 19 apeP. vivax-likestrains originating from 24 different countries across the world. We identified 2,005,455 high quality single-nucleotide polymorphism loci allowing us to conduct an extensive characterization to date ofP. vivaxworldwide genetic variation. Phylogenetic relationships between human and apePlasmodiumrevealed thatP. vivaxis a sister clade ofP. vivax-like, not included within the radiation ofP. vivax-like. By investigating a variety of aspects ofP. vivaxvariation, we identified several striking geographical patterns in summary statistics as function of increasing geographic distance from Southeast Asia, suggesting thatP. vivaxmay derived from serial founder effects from a single origin located in Asia.

Published
2020
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14. Mosquito microevolution drives Plasmodium falciparum dynamics

Author

Priscila Bascunan, Assetou Diarra, Parfait Awono-Ambene, Roch K. Dabiré, Modibo Mariko, Isabelle Morlais, Elena A. Levashina, Evans K. Rono, Mouctar Diallo, Flaminia Catteruccia, Paola Carrillo-Bustamante, Julien Pompon, Sandrine E. Nsango, Paul O. Mireji, Cynthia V. Yapto, Markus Gildenhard, Ramata Mariko, Djibril Sangaré, Anne Boissière, Hanne Krüger, Abdulaye Diabaté, Djeneba Camara, Alou Traoré, Yara Reis, Janis Thailayil, Daniel K. Masiga, Pamela B. Seda, Martin K. Rono, Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Université de Douala, Réponse immunitaire et developpement chez les insectes (RIDI - UPR 9002), Institut de biologie moléculaire et cellulaire (IBMC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d’Entomologie Médicale, Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale, Institut de Recherche en Sciences de la Santé (IRSS), CNRST, International Centre of Insect Physiology and Ecology (ICIPE), ICIPE, Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Università degli Studi di Perugia (UNIPG), Department of Epidemiology of Parasitic Diseases, Université de Bamako-Malaria Research and Training Centre, Université de Bamako, Savoirs, Textes, Langage (STL) - UMR 8163 (STL), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Institut de biologie moléculaire et cellulaire (IBMC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Transmission-Interactions-Adaptations hôtes/vecteurs/pathogènes (MIVEGEC-TRIAD), Evolution des Systèmes Vectoriels (ESV), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), and Università degli Studi di Perugia = University of Perugia (UNIPG)

Subjects
Microbiology (medical), Genotype, Rain, [SDV]Life Sciences [q-bio], Plasmodium falciparum, Immunology, Population, Mosquito Vectors, Mali, Applied Microbiology and Biotechnology, Microbiology, Plasmodium, Article, Evolution, Molecular, 03 medical and health sciences, Species Specificity, Anopheles, parasitic diseases, Prevalence, Genetics, medicine, Animals, Humans, education, Ecosystem, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 0303 health sciences, education.field_of_study, [SDV.GEN]Life Sciences [q-bio]/Genetics, biology, 030306 microbiology, Ecology, Temperature, Microevolution, Cell Biology, biology.organism_classification, medicine.disease, Malaria, 3. Good health, Genetic structure, [SDE]Environmental Sciences, Insect Proteins, Evolutionary ecology, Seasons
Abstract

Malaria, a major cause of child mortality in Africa, is engendered by Plasmodium parasites that are transmitted by anopheline mosquitoes. Fitness of Plasmodium parasites is closely linked to the ecology and evolution of its anopheline vector. However, whether the genetic structure of vector populations impacts malaria transmission remains unknown. Here, we describe a partitioning of the African malaria vectors into generalists and specialists that evolve along ecological boundaries. We next identify the contribution of mosquito species to Plasmodium abundance using Granger causality tests for time-series data collected over two rainy seasons in Mali. We find that mosquito microevolution, defined by changes in the genetic structure of a population over short ecological timescales, drives Plasmodium dynamics in nature, whereas vector abundance, infection prevalence, temperature and rain have low predictive values. Our study demonstrates the power of time-series approaches in vector biology and highlights the importance of focusing local vector control strategies on mosquito species that drive malaria dynamics. A combination of genetic analyses of Anopheles species across sub-Saharan Africa, time-series quantification of Plasmodium falciparum prevalence in mosquitoes and Granger causality statistical testing reveals that changes in the genetic structure of a population over short ecological timescales drive Plasmodium dynamics in nature.

Published
2019
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15. Cell-free and intracellular nucleic acids: new non-invasive biomarkers to explore male infertility

Author

Amaël Petiton, T. Mullet, Solenne Baillet, Samir Hamamah, Anna Gala, Antoine Torre, Alice Ferrieres-Hoa, Anne Boissière, Développement embryonnaire précoce humain et pluripotence EmbryoPluripotency (UMR 1203), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-CHU Montpellier, and Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)

Subjects
0301 basic medicine, medicine.medical_specialty, Infertilité masculine, Urology, Reproductive medicine, Cell free, Review Article, piRNA, Biology, Bioinformatics, Male infertility, [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], microRNA, medicine, Seminal plasma, miRNA, Biomarqueurs, lcsh:R5-920, 030219 obstetrics & reproductive medicine, Non invasive biomarkers, biomarkers, Human physiology, medicine.disease, 3. Good health, cf-mRNA, cf-DNA, Plasma séminal, 030104 developmental biology, Endocrinology, Reproductive Medicine, Nucleic acid, lcsh:Medicine (General), Intracellular
Abstract

Male infertility is a devastating problem that affects many couples worldwide. However, the molecular mechanisms and causes of idiopathic male infertility remain unclear. Circulating cell-free nucleic acids have an important role in human physiology and emerging evidence suggests that they play a role in male infertility. This review summarizes recent results on cell-free and intracellular nucleic acids in male infertility and discusses their potential use as biomarkers of male infertility in the clinical practice.L’infertilité masculine est un problème qui touche de nombreux couples. Cependant, aujourd’hui les mécanismes moléculaires et les causes de l’infertilité masculine idiopathique ne sont pas élucidés. Les acides nucléiques circulant ont un rôle important dans la physiologie et des évidences suggèrent qu’ils jouent un rôle dans l’infertilité masculine. L’objectif de cette revue est de mettre en avant les nouvelles avancées scientifiques sur les acides nucléiques circulant et non-circulant en lien avec l’infertilité masculine et de fournir une vue d’ensemble de leurs utilisation comme biomarqueurs en pratique clinique.

Published
2017
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16. Correction: Modulation of Malaria Infection in Anopheles gambiae Mosquitoes Exposed to Natural Midgut Bacteria

Author

Anne Boissière, Parfait Awono-Ambene, Antoine Berry, Sandrine E. Nsango, Thomas S. Churcher, Isabelle Morlais, Geoffrey Gimonneau, Richard Christen, Luc Abate, and Majoline T. Tchioffo

Subjects
Veterinary medicine, Multidisciplinary, biology, Science, Anopheles gambiae, Correction, Midgut, biology.organism_classification, medicine.disease, parasitic diseases, medicine, Medicine, Parasite hosting, Bacteria, Malaria
Abstract

There were multiple errors in the second paragraph of the Effect of bacterial challenge on P. falciparum development section of the Results: "When efficacy was measured as changes in oocyst intensity, the bacterial challenge had no effect irrespective of the oocyst intensity in the control group (Figure 3C). However, the reduction in oocyst prevalence due to the bacterial exposure was lower when parasite exposure was higher – in feedings that gave rise to the highest oocyst intensities in the PBS control, the bacterial challenge had almost no effect (Figure 3B)." In addition, an affiliation of the ninth author is missing: In addition to institution number 7, Antoine Berry is also affiliated with the the following institution: Centre de Physiopathologie de Toulouse-Purpan, Institut National de la Sante et de la Recherche Medicale, UMR1043, CNRS UMR5282, Universite de Toulouse Paul Sabatier, Toulouse, France.

Published
2013

17. Evaluation of a real-time quantitative PCR to measure the wild Plasmodium falciparum infectivity rate in salivary glands of Anopheles gambiae

Author

Alexandra Marie, Sylvie Cornelie, Anne Boissière, Anne Poinsignon, Majoline Tchioffo Tsapi, Franck Remoue, Isabelle Morlais, Parfait Awono-Ambene, Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Transmission-Interactions-Adaptations hôtes/vecteurs/pathogènes (MIVEGEC-TRIAD), Evolution des Systèmes Vectoriels (ESV), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale (OCEAC), Vector Control Group (MIVEGEC-VCG), Centre de Recherche Entomologique de Cotonou (CREC), Ministère de la Santé, Savoirs, Textes, Langage (STL) - UMR 8163 (STL), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Laboratoire d’Entomologie Médicale, Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale, Épidémiologie et prévention : environnement et efficacité des interventions (EPIPREV), Laboratoire de Recherche sur le Paludisme, Organization de Coordination pour la lutte contre les Endémies en Afrique Centrale (OCEAC), and Organization de Coordination pour la lutte contre les Endémies en Afrique Centrale

Subjects
Anopheles gambiae, [SDV]Life Sciences [q-bio], 030231 tropical medicine, Plasmodium falciparum, Real-Time Polymerase Chain Reaction, Sensitivity and Specificity, Parasite Load, Salivary Glands, 03 medical and health sciences, Quantitative PCR, 0302 clinical medicine, CSP, Anopheles, Multiplex polymerase chain reaction, parasitic diseases, medicine, Gametocyte, Animals, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 0303 health sciences, biology, Salivary gland, Methodology, Multiplex PCR, biology.organism_classification, Virology, Molecular biology, 3. Good health, Circumsporozoite protein, CSP-ELISA, PCR, Infectious Diseases, medicine.anatomical_structure, Real-time polymerase chain reaction, Parasitology, Female, ELISA, Entomology, Quantitative
Abstract

Background: Evaluation of malaria sporozoite rates in the salivary glands of Anopheles gambiae is essential for estimating the number of infective mosquitoes, and consequently, the entomological inoculation rate (EIR). EIR is a key indicator for evaluating the risk of malaria transmission. Although the enzyme-linked immunosorbent assay specific for detecting the circumsporozoite protein (CSP-ELISA) is routinely used in the field, it presents several limitations. A multiplex PCR can also be used to detect the four species of Plasmodium in salivary glands. The aim of this study was to evaluate the efficacy of a real-time quantitative PCR in detecting and quantifying wild Plasmodium falciparum in the salivary glands of An. gambiae. Methods: Anopheles gambiae (n=364) were experimentally infected with blood from P. falciparum gametocyte carriers, and P. falciparum in the sporozoite stage were detected in salivary glands by using a real-time quantitative PCR (qPCR) assay. The sensitivity and specificity of this qPCR were compared with the multiplex PCR applied from the Padley method. CSP-ELISA was also performed on carcasses of the same mosquitoes. Results: The prevalence of P. falciparum and the intensity of infection were evaluated using qPCR. This method had a limit of detection of six sporozoites per mu L based on standard curves. The number of P. falciparum genomes in the salivary gland samples reached 9,262 parasites/mu L (mean: 254.5; 95% CI: 163.5-345.6). The qPCR showed a similar sensitivity (100%) and a high specificity (60%) compared to the multiplex PCR. The agreement between the two methods was "substantial" (k = 0.63, P < 0.05). The number of P. falciparum-positive mosquitoes evaluated with the qPCR (76%), multiplex PCR (59%), and CSP-ELISA (83%) was significantly different (P < 0.005). Conclusions: The qPCR assay can be used to detect P. falciparum in salivary glands of An. gambiae. The qPCR is highly sensitive and is more specific than multiplex PCR, allowing an accurate measure of infective An. gambiae. The results also showed that the CSP-ELISA overestimates the sporozoite rate, detecting sporozoites in the haemolymph in addition to the salivary glands.

Published
2013
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18. Modulation of malaria infection in Anopheles gambiae mosquitoes exposed to natural midgut bacteria

Author

Geoffrey Gimonneau, Richard Christen, Isabelle Morlais, Sandrine E. Nsango, Antoine Berry, Anne Boissière, Majoline T. Tchioffo, Thomas S. Churcher, Parfait Awono-Ambene, Luc Abate, Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Laboratoire de Recherche sur le Paludisme, Organization de Coordination pour la lutte contre les Endémies en Afrique Centrale (OCEAC), Organization de Coordination pour la lutte contre les Endémies en Afrique Centrale, Imperial College London, Symbiose Marine (SM), Evolution Paris Seine, Université des Antilles et de la Guyane (UAG)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles et de la Guyane (UAG)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Centre de Physiopathologie de Toulouse Purpan, Institut National de la Recherche Agronomique (INRA), Institut de Recherche pour le Developpement (IRD), Agence Nationale de la Recherche [ANR-11-BSV7-009-01], APEGE programme from the Institut Ecologie et Environnement, European Union [242095- EVIMalaR, 223601- MALVECBLOK], Infectiopole Sud foundation, IRD, Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale (OCEAC), Université des Antilles et de la Guyane (UAG)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles et de la Guyane (UAG)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Nice Sophia Antipolis (1965 - 2019) (UNS), and Medical Research Council (MRC)

Subjects
Anopheles gambiae, DIVERSITY, Colony Count, Microbial, INDEPENDENT METHODS, L73 - Maladies des animaux, Plasmodium, Interactions biologiques, Hôte, 0302 clinical medicine, RNA, Ribosomal, 16S, Cameroon, Malaria, Falciparum, VERTICAL TRANSMISSION, 0303 health sciences, Multidisciplinary, PLASMODIUM-FALCIPARUM, [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE], Anopheles, Facteur du milieu, MICROBIOTA, 3. Good health, Épidémiologie, Multidisciplinary Sciences, Flore intestinale, Science & Technology - Other Topics, Medicine, SPOROGONIC DEVELOPMENT, Infection, L72 - Organismes nuisibles des animaux, Research Article, Genre humain, Symbiotic bacteria, General Science & Technology, Relation hôte parasite, Science, Molecular Sequence Data, Plasmodium falciparum, 030231 tropical medicine, Biology, Microbiology, 03 medical and health sciences, CULICIDAE, SYMBIONTS, parasitic diseases, Escherichia coli, Animals, Humans, Microbiome, PARASITE, 030304 developmental biology, Science & Technology, Bacteria, Base Sequence, Flore microbienne, Oocysts, Genetic Variation, Midgut, biology.organism_classification, Malaria, DIPTERA, Digestive System
Abstract

International audience; The development of Plasmodium falciparum within the Anopheles gambiae mosquito relies on complex vector-parasite interactions, however the resident midgut microbiota also plays an important role in mediating parasite infection. In natural conditions, the mosquito microbial flora is diverse, composed of commensal and symbiotic bacteria. We report here the isolation of culturable midgut bacteria from mosquitoes collected in the field in Cameroon and their identification based on the 16S rRNA gene sequencing. We next measured the effect of selected natural bacterial isolates on Plasmodium falciparum infection prevalence and intensity over multiple infectious feedings and found that the bacteria significantly reduced the prevalence and intensity of infection. These results contrast with our previous study where the abundance of Enterobacteriaceae positively correlated with P. falciparum infection (Boissiere et al. 2012). The oral infection of bacteria probably led to the disruption of the gut homeostasis and activated immune responses, and this pinpoints the importance of studying microbe-parasite interactions in natural conditions. Our results indicate that the effect of bacterial exposure on P. falciparum infection varies with factors from the parasite and the human host and calls for deeper dissection of these parameters for accurate interpretation of bacterial exposure results in laboratory settings.

Published
2013
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19. Application of a qPCR assay in the investigation of susceptibility to malaria infection of the M and S Molecular Forms of An. gambiae s.s. in Cameroon

Author

Geoffrey Gimonneau, Parfait Awono-Ambene, Luc Abate, Sandrine E. Nsango, Majoline T. Tchioffo, Anne Boissière, Isabelle Morlais, Albert N. Bayibéki, Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Laboratoire de Recherche sur le Paludisme, Organization de Coordination pour la lutte contre les Endémies en Afrique Centrale (OCEAC), Organization de Coordination pour la lutte contre les Endémies en Afrique Centrale, Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale (OCEAC), Savoirs, Textes, Langage (STL) - UMR 8163 (STL), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement (IRD), Transmission-Interactions-Adaptations hôtes/vecteurs/pathogènes (MIVEGEC-TRIAD), Evolution des Systèmes Vectoriels (ESV), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Laboratoire d’Entomologie Médicale, Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale, and Université de Douala

Subjects
Epidemiology, Anopheles gambiae, [SDV]Life Sciences [q-bio], lcsh:Medicine, law.invention, 0302 clinical medicine, law, Genotype, lcsh:Science, Polymerase chain reaction, ComputingMilieux_MISCELLANEOUS, 0303 health sciences, Multidisciplinary, Ecology, biology, Anopheles, 3. Good health, Épidémiologie, Infectious Diseases, PCR, Sympatric speciation, S50 - Santé humaine, Medicine, Infection, L72 - Organismes nuisibles des animaux, Research Article, 030231 tropical medicine, Plasmodium falciparum, Allopatric speciation, Infectious Disease Epidemiology, 03 medical and health sciences, Analyse quantitative, parasitic diseases, Genetics, Parasitic Diseases, medicine, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Biology, Transmission des maladies, 030304 developmental biology, Population Biology, lcsh:R, Tropical Diseases (Non-Neglected), Morbidité, biology.organism_classification, medicine.disease, Virology, Malaria, lcsh:Q, U30 - Méthodes de recherche
Abstract

Plasmodium falciparum is the causative agent of malaria, a disease that kills almost one million persons each year, mainly in sub-Saharan Africa. P. falciparum is transmitted to the human host by the bite of an Anopheles female mosquito, and Anopheles gambiae sensus stricto is the most tremendous malaria vector in Africa, widespread throughout the afro-tropical belt. An. gambiae s.s. is subdivided into two distinct molecular forms, namely M and S forms. The two molecular forms are morphologically identical but they are distinct genetically, and differ by their distribution and their ecological preferences. The epidemiological importance of the two molecular forms in malaria transmission has been poorly investigated so far and gave distinct results in different areas. We have developed a real-time quantitative PCR (qPCR) assay, and used it to detect P. falciparum at the oocyst stage in wild An. gambiae s.s. mosquitoes experimentally infected with natural isolates of parasites. Mosquitoes were collected at immature stages in sympatric and allopatric breeding sites and further infected at the adult stage. We next measured the infection prevalence and intensity in female mosquitoes using the qPCR assay and correlated the infection success with the mosquito molecular forms. Our results revealed different prevalence of infection between the M and S molecular forms of An. gambiae s.s. in Cameroon, for both sympatric and allopatric populations of mosquitoes. However, no difference in the infection intensity was observed. Thus, the distribution of the molecular forms of An. gambiae s.s. may impact on the malaria epidemiology, and it will be important to monitor the efficiency of malaria control interventions on the two M and S forms.

Published
2013
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20. OPA1 mutations induce mitochondrial DNA instability and optic atrophy plus phenotypes.

Author

Patrizia Amati-Bonneau, Maria Lucia Valentino, Pascal Reynier, Maria Esther Gallardo, Belén Bornstein, Anne Boissière, Yolanda Campos, Henry Rivera, Jesús González de la Aleja, Rosanna Carroccia, Luisa Iommarini, Pierre Labauge, Dominique Figarella-Branger, Pascale Marcorelles, Alain Furby, Katell Beauvais, Franck Letournel, Rocco Liguori, Chiara La Morgia, and Pasquale Montagna

Subjects
DNA, APOPTOSIS, CELL death, MUSCLE diseases
Abstract

Mutations in OPA1, a dynamin-related GTPase involved in mitochondrial fusion, cristae organization and control of apoptosis, have been linked to non-syndromic optic neuropathy transmitted as an autosomal-dominant trait (DOA). We here report on eight patients from six independent families showing that mutations in the OPA1 gene can also be responsible for a syndromic form of DOA associated with sensorineural deafness, ataxia, axonal sensory-motor polyneuropathy, chronic progressive external ophthalmoplegia and mitochondrial myopathy with cytochrome c oxidase negative and Ragged Red Fibres. Most remarkably, we demonstrate that these patients all harboured multiple deletions of mitochondrial DNA (mtDNA) in their skeletal muscle, thus revealing an unrecognized role of the OPA1 protein in mtDNA stability. The five OPA1 mutations associated with these DOA ‘plus’ phenotypes were all mis-sense point mutations affecting highly conserved amino acid positions and the nuclear genes previously known to induce mtDNA multiple deletions such as POLG1, PEO1 (Twinkle) and SLC25A4 (ANT1) were ruled out. Our results show that certain OPA1 mutations exert a dominant negative effect responsible for multi-systemic disease, closely related to classical mitochondrial cytopathies, by a mechanism involving mtDNA instability. [ABSTRACT FROM AUTHOR]

Published
2008
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21. The Anopheles Mosquito Microbiota and Their Impact on Pathogen Transmission

Author

Mathilde Gendrin, George K. Christophides, Department of Life Sciences, Imperial College London, London, United Kingdom, Imperial College London, and We thank Jiannong Xu, Jewelna Osei-Poku, Anne Boissière and Isabelle Morlais for providing example sequences of some of the bacterial genera shown in Table 1 and Thierry Lefèvre for helping with mosquito pictures presented in Figure 1.

Subjects
0303 health sciences, biology, [SDV]Life Sciences [q-bio], 030231 tropical medicine, Anopheles, Zoology, biology.organism_classification, law.invention, 03 medical and health sciences, 0302 clinical medicine, Transmission (mechanics), law, Pathogen, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology
Abstract

International audience

Published
2013
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