17 results on '"Ariani, Novita"'
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2. A validated HPLC-DAD method and comparison of different extraction techniques for analysis of scopoletin in noni-based products
- Author
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Tasfiyati, Aprilia Nur, Antika, Lucia Dwi, Septama, Abdi Wira, Hikmat, Hikmat, Kurniawan, Hendris Hendarsyah, and Ariani, Novita
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- 2023
- Full Text
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3. Antibacterial Activity from The Cashew Nut Shell Extracts
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Abbas Jamilah, Ariani Novita, and Andayanie Wuye Ria
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Environmental sciences ,GE1-350 - Abstract
Cashew nut shells were considered as a waste material of the cashew industries; therefore, we used this material as a source of antibacterial drugs and as a source of chemical constituent. Anacardium accidentale Linn (Anacardiacea family) popularly known as “Cashew”, is grown in the sandy loam soil, and has a spreading root system. Nutshell from cashew nut represents one of the major cheapest sources of non-isoprenoid phenolic lipid. This study investigated of antibacterial activities of cashew nut shell extract (CNSE). Samples were maceration by hexane, dichloromethane, ethyl acetate, methanol, and water solvent were then evaporated at 50 oC to give n-hexane, dichloromethane, ethyl acetate, methanol, and water extract. The yield were obtained was 125.54 gr (28.91 %), 11.19 gr (2.58 %), 43.15 gr (9.94 %), 2.46 gr (0.08 %), and 46.50 gr (10.71 %), for hexane, dichloromethane, ethyl acetate, methanol, and water solvent espectively. The extract was examined for bacterias, namely Bacillus subtillis, Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus, using the agar disc diffusion methods in using concentration of Cashew nut shell of 0.125%. 0.25%, 0.5 % and 1% respectively. The ethyl acetate extract and n-hexane extract of CNSE showed an antibacterial effect on B. subtillis with an inhibition zone of 10.333 mm and 9.67 mm. An n-hexane extract showed inhibition zone (11.667 mm) for S. aureus, 11.33 mm for P. aeruginosa. Methanol extract exhibited inhibition zone higher for S. aureus, followed by E. coli, P. aeruginosa and B. subtillis. Water extract did not affect the growth of E. coli. Four the ethyl acetate, methanol, hexane, and dichloromethane extract showed better antibacterial effects compared to water extract. Chemical constituents of hexane extract of cashew nut shell were investigated by GC-MS.
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- 2024
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4. Potential Antioxidant Activity Methods DPPH, ABTS, FRAP, Total Phenol and Total Flavonoid Levels of Macaranga hypoleuca (Reichb. f. & Zoll.) Leaves Extract and Fractions
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Minarti Minarti, Ariani Novita, Megawati Megawati, Hidayat Arief, Hendra Medi, Primahana Gian, and Darmawan Akhmad
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Environmental sciences ,GE1-350 - Abstract
The Macaranga species (Euphorbiaceae), also known locally as "mahang", has been used in traditional medicine due to its potential health benefits. Around 12 species of the Macaranga have been found to contain more than 50 flavonoid compounds, which are the primary secondary metabolites in this genus. Free radicals can lead to organ damage, resulting in various chronic and degenerative disorders. Active antioxidant compounds can help inhibit the oxidation process caused by free radicals, and phenolic compounds, mainly from the flavonoid group, are thought to be present in Macaranga hypoleuca as a source of natural antioxidants. This study aimed to identify the antioxidant potential of methanol extract and its fractions of M. hypoleuca using the DPPH, ABTS and FRAP methods using a UV-Vis spectrometer method, as well as to determine total phenol and total flavonoid levels. The highest antioxidant activity of the DPPH and ABTS methods was found in the ethyl acetate fraction with IC50 values of 14.31 and 2.10 mg/L respectively. Meanwhile, the highest antioxidant activity of the FRAP method was found in the butanol fraction with an IC50 value of 0.48 mg/L. The highest total phenol and flavonoid content was found in the butanol fraction at 27.51 mg GAE/g and 88.42 mg QE/g respectively.
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- 2024
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5. Activities of Horsfieldia spicata (Roxb.) J. Sinclair bark extract and fraction as α-glucosidase inhibitor.
- Author
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Megawati, Megawati, Ariani, Novita, Minarti, Minarti, Darmawan, Akhmad, Fajriah, Sofa, and Hidayat, Arif
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PHYTOCHEMICALS , *ALPHA-glucosidases , *PHENOLS , *FLAVONOIDS , *ETHYL acetate , *TRADITIONAL medicine , *EXTRACTS - Abstract
Myristicaceae known as one of the plants that widely used as traditional medicine. Utilization of Horsfieldia spicata (Roxb.) J. Sinclair, one of the Myristicaceae family member as a sources of antidiabetic active compounds are still needed. The antidiabetic activity of methanol extract (ME) and its fractions [hexane fraction (HF), ethyl acetate fraction (EF), butanol fraction (BF) and residue fraction (RF)] of H. Spicata stem bark were in vitro tested by measuring of screening of phytochemical contents, total phenolic and flavonoid compounds, and α-glucosidase enzyme inhibitory activity. Phytochemical screening result showed that (ME) indicated the presence of flavonoid and saponin compounds. Due to the total phenolic and flavonoid compounds measurement result, indicated that ME has a highest value for total phenolic compounds (14.709 ± 0.23 µg/mL) and EF for total flavonoid compounds (55.825 ± 2.21 µg/mL). Antidiabetic activity of the ME, HF, EF, BF, and RF against α-glucosidase enzyme showed that ME has antidiabetic activity with IC50 value 49.30 µg/mL, which is it was higher than HF, BF and RF, but less active compared to EF and quercetin (positive control) with the IC50 value are 159.64, 50.60, 94.01, 22.73, 2.99 µg/mL, respectively. The result showed that (EF) of methanolic extract of H. Spicata has inhibitory activity against α-glucosidase enzyme, and potential to be develop as antidiabetic active fraction. [ABSTRACT FROM AUTHOR]
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- 2023
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6. Mercury monitoring in the skin care product in South Tangerang Indonesia using direct mercury analyzer.
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Kurniawan, Hendris Hendarsyah, Ariani, Novita, Muzdalifah, Dian, and Hikmat
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SKIN care products , *MERCURY , *SKIN care - Abstract
Skin care products that give the appearance of Korean skin are in increasing demand in Indonesia. Perception of beauty may result in desires to have dewy skin with lighter tones instantly. One of the dangerous ingredients that is often added to skin lightening is mercury. Mercury is added to skin care products as a skin-bleaching agent to inhibits melanin production. This study has been successfully determinate mercury exposure in 15 skin care products that commonly used to get dewy and brighter skin. Mercury determination is carried out using direct mercury analyzer, which is a simple mercury detector that thermally decomposes the sample to release mercury which will be captured by amalgamator and measured with a spectrometer at 253.7 nm. Validation parameters used were linearity, Limit of Detection (LoD), Limit of Quantitation (LoQ), precision and accuracy. Linearity, used standard solution of 0.5 – 25 µg/kg, was obtained from concentrations of 2 to 15 µg/kg and it produced regression equation y=0.0338x+0.0183 with r2 0.9996. The Limit of Detection (LoD) and the Limit of Quantitation (LoQ) in this study were 0.15 and 0.46 µg/kg, respectively. The precision was determined by %RSD with the result 4.66 %. Accuracy used recovery of spiked standard, the % recovery result is 99.49 %. The results of measurement showed that no mercury founded in tested skin care products, it is also under threshold set by Food and Drug Supervisory Agency of the Republic of Indonesia, <1.0 mg/kg or <1.0 mg/L. Use of skin care that contains mercury must be reduced due to prolonged exposure to mercury through repeated skin care applications may cause mercury accumulation in the body tissues leading to the impairment of brain, nervous and renal systems. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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7. Asarinin, a furofuran lignan compound isolated from Horsfieldia spicata.
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Darmawan, Akhmad, Megawati, Megawati, Ariani, Novita, Mujahidin, and Hidayat, Arief
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SESAMIN ,NEOLIGNANS ,LIGNANS ,ETHYL acetate ,SILICA gel ,COLUMN chromatography ,CHEMICAL structure - Abstract
Our continuous investigation of the chemical constituent of the leaves of Horsfieldia spicata (Myristicaceae) led to the isolation of asarinin, a furofuran lignan compound. H. spicata dried leaves were macerated with methanol to obtained methanol extract (ME), ME further fractionated with n-hexane (HF), ethyl acetate (EAF) and butanol (BF). EAF subjected to column chromatography (CC)(silica gel 0.2-0.5 mm, gradient n-hexane/ethyl acetate/methanol with 5% increase in polarity), a white needle crystal successfully isolated from sub-fraction of HDEA-SF 2. The chemical structure of asarinin was elucidated based on spectroscopic data collected from LC-MS and NMR data (1D-and 2D-NMR). [ABSTRACT FROM AUTHOR]
- Published
- 2023
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8. Preliminary investigation of antibiotic potential from esters of citronella oil derivatives.
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Widiyarti, Galuh, Filailla, Euis, Ariani, Novita, Mulyani, Hani, Minarti, Minarti, Ernawati, Teni, and Laksmono, Joddy Arya
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DISC diffusion tests (Microbiology) ,ANTIBIOTICS ,ANTIBIOTIC overuse ,ESTERS ,DRUG resistance in bacteria ,CANDIDA albicans ,AGAR - Abstract
Microbial resistance due to the overuse of antibiotics has become a global health problem. The discovery and development of new active compounds to solve the antimicrobial resistance problems are urgently required. This research was conducted to study the antimicrobial potential of citronellyl isobutyrate (CIB) and geranyl isobutyrate (GIB). CIB and GIB are derivatives of citronella oil extracted from the lemongrass plant, one of the Indonesian native medicinal plants. The esters'antimicrobial was analyzed in vitro by antibacterial and antifungal tests using the agar diffusion method and compared to streptomycin sulfate as a standard. The minimum inhibitory concentration (MIC) was then evaluated using a serial microdilution assay. The esters exhibited moderate to vigorous antimicrobial activity against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Candida albicans, with activity index (AI) values between 34.17-93.59%. The results revealed that the compounds have the potential to be developed as antibiotics. Therefore, this study is expected to help overcome the problem of antibiotic resistance. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
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9. An ethnopharmacology study of Indonesian medicinal plants in Gunung Sari village as dipeptidyl peptidase-IV inhibitor
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Swandiny, Greesty Finotory, Primahana, Gian, Prastya, Muhammad Eka, Ariani, Novita, Susanti, Deny, Hanafi, Muhammad, and Abdillah, Syamsudin
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dipeptidyl peptidase-IV ,Pamijahan ,Piper ornatum ,ethnopharmacology study - Abstract
During an ethnopharmacology study of traditional antidiabetic treatment in Gunung Sari village, Bogor region, Indonesia, fifteen traditional medicinal plants were selected, collected and prepared as crude extracts. Among fifteen plants, only three plants have previously been screened for dipeptidyl peptidase-IV (DPP-IV) inhibitors. Quantitative phytochemical analysis revealed total phenolics content (TPC) ranging from 2.27±0.16 to 5.39±0.05 mg GAE/g extract and total alkaloids content (TAC) from 1.07±0.02 to 4.33±0.07 mg QE/g extract. In-vitro DPP-IV inhibitory activity screening showed that Piper ornatum exhibited the highest inhibition (78.11±1.35 %) and the lowest activity by Syzygium polyanthum (34.30±1.57%) at a concentration of 250 µg/mL, respectively. Analysis of chemical constituents using liquid chromatography-high resolution mass spectrometry (LC-HRMS) indicated at least eleven compounds were present in the crude extract. Among them, several peaks were tentatively assigned as pipcrosides and crocatins, which have previously been isolated from Piper crocatum.
- Published
- 2023
10. Chemical Composition, Antioxidant, Antibacterial, Antibiofilm, and Cytotoxic Activities of Robusta Coffee Extract (Coffea canephora).
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Suryanti, Erma, Retnowati, Dwi, Prastya, Muhammad Eka, Ariani, Novita, Yati, Indri, Permatasari, Vera, Mozef, Tjandrawati, Dewijanti, Indah D., Yuswan, Apriza, Asril, Muhammad, Riana, Elisa Nurma, and Batubara, Irmanida
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COFFEE ,FLAVONOIDS ,FUNCTIONAL foods ,GRAM-positive bacteria ,BACTERIAL growth ,PLANT phenols ,FRUIT extracts ,BACILLUS subtilis - Abstract
Coffee extracts are a rich source of potential compounds with numerous biological activities. This study aimed to investigate the potential of chemical compounds derived from robusta coffee extract (Coffea canephora) and its dominant compound, caffeine, to scavenge free radicals, inhibit bacterial growth and biofilm formation, and cytotoxic properties in human breast adenocarcinoma cell line. Chemical constituents of coffee extract were analysed quantitively for total phenolic, flavonoid, and alkaloid contents, along with Liquid Chromatography Quadrupole-Mass Spectrometry (LC/MS–MS) analysis. Antioxidant activity was measured by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis; 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) methods. Antibacterial pair with antibiofilm properties against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus were also determined, as well as cytotoxic activity on the MCF7 cell line model. The LC/MS–MS analysis of coffee extracts revealed high levels of caffeine; thus, the caffeine standard is used in all subsequent assays. Notably, robusta coffee extract showed remarkable antioxidant activity and selective inhibition of the growth against gram-positive and negative bacteria, along with the best inhibition of P. aeruginosa biofilm. However, compared with the caffeine standard, robusta coffee extract had lower cytotoxic activity and different bacterial targets in antibacterial and antibiofilm properties. Our results indicate that Robusta coffee extract is potentially a functional food due to its high alkaloid, phenolic, and flavonoid content and antioxidant activity, besides being used for natural compounds against bacterial infections. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
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11. Investigations of Antibacterial, Antioxidant, and Antidiabetic Potential of Extract and Its Active Fractions from the Leaves of Horsfieldia spicata (Roxb.) J. Sinclair.
- Author
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Megawati, Ariani, Novita, Minarti, Darmawan, Akhmad, and Eka Prastya, Muhammad
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- 2023
- Full Text
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12. Estimation of uncertainty measurement for quantitative analysis of endosulfan sulfate in black tea using gas chromatography-electron capture detector.
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Ramadhaningtyas, Dillani Putri, Aristiawan, Yosi, Styarini, Dyah, Kurniawan, Hendris Hendarsyah, Ariani, Novita, and Muzdalifah, Dian
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QUANTITATIVE research ,ENDOSULFAN ,SULFATES ,DETECTORS ,TEA - Abstract
In ensuring food safety, crucial and strategic policies are taken based on scientific information and analytical measurements obtained from the standard and validated methods. Therefore, accurate and high-quality measurements are essential to ensure that the results align with the regulation. One of the parameters that can be assessed for the reliability of an analytical method is its estimated measurement uncertainty. In this work, the measurement uncertainty for quantitative analysis of endosulfan sulfate in black tea using GC-ECD was estimated based on a bottom-up approach according to EURACHEM/CITAC GUIDE, considering the uncertainty from sample preparation, calibration curve, standard solutions, recovery, repeatability, and water content. The results showed that the main sources of combined measurement uncertainty were calibration curve, recovery, and repeatability. The expanded measurement uncertainty was calculated at around 12% (coverage factor k=2, confidence level 95%) for the sample's concentration of 527 µg/kg. Comparing this value and CV Horwitz showed that the estimation was reasonable and demonstrated its fitness for purpose. [ABSTRACT FROM AUTHOR]
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- 2022
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13. Optimatization in Bioconversion of Quercetin Glucosides Using Aspergillus acueletus LS04-3
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Dewi, Rizna Triana, Ariani, Novita, Ekapratiwi, Yasmin, and Filaila, Euis
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heterocyclic compounds ,Chemistry ,Natural product and pharmacy - Abstract
Microbial transformation is known to be useful for obtaining more active or less toxic compounds and achieving selective compounds conversion to more useful derivatives. The objective of this study is to explore several factors that affect the optimization of bioconversion of quercetin glucosides using Aspergillus acueletus LS04-3. To obtain the optimum condition of biotransformation the fungus was cultured under several conditions by varying the number of days of fermentation, the concentration of substrate, carbon, and nitrogen source. The transformation product was analyzed using HPLC and LCMS-MS. The results revealed that on the third-day biotransformation process, quercetin production reached the highest amount and the optimum concentration was at 50 ppm of quercetin glucoside based on HPLC analysis. In addition, from various carbon sources, glucose yielded the highest biotransformation product, while nitrogen accelerated the reaction. In this research, media of A. acueletus LS04-3 containing carbon and nitrogen could increased quercetin production. This research finding provided several factors for optimizing quercetin biotransformation by A. acueletus LS04-3. These factors were important in scaling up biotransformation even in other biotransformation reactions.
- Published
- 2021
14. Bioconversion of Quercetin Glucosides from Dendrophthoe pentandra leaf Using Aspergillus acueletus LS04-3.
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Dewi, Rizna Triana, Ekapratiwi, Yasmin, Sundowo, Andini, Ariani, Novita, Yolanda, Tria, and Filaila, Euis
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QUERCETIN ,GLUCOSIDES ,BIOCONVERSION ,ENERGY metabolism ,ASPERGILLUS ,FILAMENTOUS fungi - Abstract
Mistletoe (Dendrophthoe pentandra Miq.) leaves serve as a herbal medicine, particularly used in Indonesian folk medicine, for treating various immunological disorders and cancers. One major active compound of mistletoe plant was flavonoid that occured in the form of glycosides (quercetin-3-O-rhamnoside) instead of aglycone (quercetin). Quercetin from the flavonoid group had high bioactivities than that in glycosidic form. The fermentation of flavonoid glycoside with filamentous fungi resulted in the conversion of bioactive compounds. The objectives of this study were to explore the derivatives of quercetin-3-O-rhamnoside during fungal fermentation of water-extracted mistletoe (D. pentandra Miq.) leaves (B) by A. acueletus LS04-3 and investigate the influence of glucose (B+G), yeast (B+Y), and both glucose and yeast (B+G+Y) in the medium on the metabolism of quercetin-3-O-rhamnosidebased on HPLC and LCMS-MS analysis. Based on HPLC analysis, the addition of yeast to the medium (B+Y) increased the conversion of glucoside to quercetin almost twice as much as that on the control medium (B), during the first day of incubation period (D-1). The 10% increase of quercetin peak was only seen on the third day of incubation in B+G medium, when compared to the control sample (B). Moreover, LCMS-MS analysis revealed that quercetin-3-O-rhamnoside and quercetin were detected as the major components in fermented mistletoe leaves. Furthermore, quercetin-3-galactoside-7-glucoside and robinetin were found in the medium that had been added with glucose. The study suggest that glycosilation could also occur in processes other than hydrolisis of glycoside because external glucose would affect the energy metabolism and sugar activation of the microbe. Our finding expanded the understanding of the mistletoe leaf fermentation process and provided further guidance for the biotransformation of quercetin-3-O-rhamnoside in scale up production. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
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15. Performance evaluation and operation cost analysis of electrolytes application in electrocoagulation process applied to peat wastewater treatment.
- Author
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Sari, Ajeng Arum, Suwanto, Nandar, Asmara, Adelia Anju, Ariani, Novita, Setiawan, Arief A. R., Waluyo, Joko, Muryanto, and Sudarno
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ELECTROLYTE analysis ,WASTEWATER treatment ,COST analysis ,ELECTROCOAGULATION (Chemistry) ,PEAT - Abstract
In order to investigate the performance and cost analysis of electrocoagulation process, various electrolytes are picked out to remove Fe, color, and turbidity in peat water through batch and continuous reactors. Peat water has brownish red color, acidity, high turbidity, and a large concentration of Fe which exceed the water standard quality. Electrocoagulation is an electrochemical method for water treatment that along the anode where active coagulant as aluminum ions is released into the solution. Several kinds of electrolytes are applied, such as 0.01M NaCl p.a., 0.01M NaCl technical grade, 0.01M NH
4 Cl p.a., and 0.01M K2 SO4 p.a. with process time up to 120 minutes. The results show that the electrocoagulation in a batch process using NH4 Cl for 120 minutes obtains the highest of removal percentage of Fe, color, and turbidity, which are 90.92%, 84.93%, and 99.94%, respectively. However, according to scoring evaluation, commercial NaCl using through continuous reactor generates the outputs have fulfilled the water standard quality as well as batch condition with low-cost production about Rp 23.21/Liter, about 64.18% cheaper than without electrolytes addition, and 82.93% more inexpensive than generally chemical treatment. [ABSTRACT FROM AUTHOR]- Published
- 2019
- Full Text
- View/download PDF
16. Identification Of Di(ethylhexyl) Phthalate As Impurity In The Analysis By Using Chromatography Gas Tandem Mass Spectrometry.
- Author
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Pusfitasari, Ek Dian, Hendarsyah, Hendris, Salahuddin, and Ariani, Novita
- Subjects
DIETHYLHEXYL phthalate ,VAPOR pressure ,GAS chromatography/Mass spectrometry (GC-MS) ,FOOD chemistry ,SELECTED ion monitoring - Abstract
Di(elhylhexyl) phthalate (DEHP) is a plastieizer eommonly used in plasties. Physieally DEHP has a low vapor pressure. DEHP ean seep into the liquid in direct eontaet with the plastic wrapping materials, and typically can occur rapidly if extractable into food or non-polar solvents, such as oil, once the food is packaged in PVC packaging materials. DEHP has been analyzed by using gas chromatography which has a high sensitivity level. If the equipment used for the analysis is made from plastic containing DEHP, then it may be possible that DEHP can be extracted and appear on the outcome of the injection. It can interfere with the process of analysis, especially for the analysis of food samples. This study has identified the present of DEHP in the blank injection performed by Gas Chromatography tandem Mass Spectrometry with Selected Ion Monitoring mode SIM). Researchers are required to verify whether the gas chromatographic system used is ready for the analysis process. In addition, the comparison and calculation of the intensity of the ion fragmentation spectra generated by mass spectrometry detector can be used for the qualitative determination to ensure the presence of the target compound. In this study is also discussed the differences between the high-intensity fragmentation of DEHP and dioctyl phthalate (DOP). [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
17. Ligninolytic Enzymes Production for Degradation of Organochlorine Compound by Trametes versicolor U80.
- Author
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San, Ajeng Arum, Ariani, Novita, Saiiro Tachibana, and Sudiyam, Yamn
- Subjects
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PHYSIOLOGICAL effects of organochlorine compounds , *TRAMETES versicolor , *CHEMICAL decomposition , *TRAMETES (Polyporaceae) , *MANUFACTURING processes - Abstract
A variety of industrial processes involving chlorine and carbon produce pentachlorobenzene as by-product which is emitted into the environment. The presence of pentachlorobenzene in the environment becomes a great concern due to its persistent, long-range fluid nature and toxic biological effects. In this study, the potential of newly basidiomycetes fungal strain to degrade pentachlorobenzene in liquid medium was evaluated. This study also investigated the enzymatic activities involving the degradation of pentachlorobenzene by fungal strain. These experiments were conducted in agar and liquid media. On agar medium containing pentachlorobenzene, five fungi showed the different abilities of growth with range 40 % to 63 % where strain U80 showed the highest growth on this medium. In morphology analysis, USO had short cottony, floccose to floccose-cortony, and white. Based on DNA analysis, U80 belongs to the genus Trametes and named Trametes versicolor U80. Trametes versicolor U80 degraded 43 % of pentachlorobenzene (0.1 mM) in liquid media for 40 d incubation in wliich the relative degradation per-unit biomass w?as similar during the time of degradation, indicating the pollutant was not toxic for the growth of fungal strain. Trametes versicolor U80 secreted higher ligninolytic enzymes on 10 d and 20 d means that catalysis of the decomposition of the recalcitrant aromatic compounds by T. versicolor U80 was affected by extracellular enzymes. The addition of EDTA and piperonyl butoxide revealed that LiP and P-450 monooxygenase handled degradation in the different time. In phytotoxicity test, this strain successfully decreased the toxicity of pentachlorobenzene. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
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