19 results on '"Atayde, Luís"'
Search Results
2. Innovative Ink-Based 3D Hydrogel Bioprinted Formulations for Tissue Engineering Applications.
- Author
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Sousa, Ana Catarina, Mcdermott, Grace, Shields, Fraser, Alvites, Rui, Lopes, Bruna, Sousa, Patrícia, Moreira, Alícia, Coelho, André, Santos, José Domingos, Atayde, Luís, Alves, Nuno, Richardson, Stephen M., Domingos, Marco, and Maurício, Ana Colette more...
- Abstract
Three-dimensional (3D) models with improved biomimicry are essential to reduce animal experimentation and drive innovation in tissue engineering. In this study, we investigate the use of alginate-based materials as polymeric inks for 3D bioprinting of osteogenic models using human bone marrow stem/stromal cells (hBMSCs). A composite bioink incorporating alginate, nano-hydroxyapatite (nHA), type I collagen (Col) and hBMSCs was developed and for extrusion-based printing. Rheological tests performed on crosslinked hydrogels confirm the formation of solid-like structures, consistently indicating a superior storage modulus in relation to the loss modulus. The swelling behavior analysis showed that the addition of Col and nHA into an alginate matrix can enhance the swelling rate of the resulting composite hydrogels, which maximizes cell proliferation within the structure. The LIVE/DEAD assay outcomes demonstrate that the inclusion of nHA and Col did not detrimentally affect the viability of hBMSCs over seven days post-printing. PrestoBlue
TM revealed a higher hBMSCs viability in the alginate-nHA-Col hydrogel compared to the remaining groups. Gene expression analysis revealed that alginate-nHA-col bioink favored a higher expression of osteogenic markers, including secreted phosphoprotein-1 (SPP1) and collagen type 1 alpha 2 chain (COL1A2) in hBMSCs after 14 days, indicating the pro-osteogenic differentiation potential of the hydrogel. This study demonstrates that the incorporation of nHA and Col into alginate enhances osteogenic potential and therefore provides a bioprinted model to systematically study osteogenesis and the early stages of tissue maturation in vitro. [ABSTRACT FROM AUTHOR] more...- Published
- 2024
- Full Text
- View/download PDF
Catalog
3. Equine Musculoskeletal Pathologies: Clinical Approaches and Therapeutical Perspectives—A Review.
- Author
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Reis, Inês L., Lopes, Bruna, Sousa, Patrícia, Sousa, Ana C., Caseiro, Ana R., Mendonça, Carla M., Santos, Jorge M., Atayde, Luís M., Alvites, Rui D., and Maurício, Ana C.
- Subjects
HORSE health ,CLINICAL pathology ,SPORTS injuries ,SPORTS medicine ,HORSE sports ,HYALURONIC acid ,HEALING - Abstract
Simple Summary: In the current era, sport horses face the challenge of increasingly rigorous workouts, overtaking, and, at times, certain physiological thresholds, leaving them susceptible to injury. This shift underscores the significance of prioritizing the exercise preparation quality and post-care to proactively mitigate the risk of injuries. Despite these measures, injuries may still occur, thus requiring a nuanced understanding of the most effective therapeutic approaches for various types of lesions. In the dynamic field of equine sports medicine, keeping abreast of the expansive therapeutic options proves challenging, especially when aiming to address injuries comprehensively, restore organ function, and sustain the horse's athletic life. The present endeavor seeks to elucidate the array of available therapies, encompassing both conservative and regenerative methods, for the effective management of musculoskeletal injuries in sport horses. Musculoskeletal injuries such as equine osteoarthritis, osteoarticular defects, tendonitis/desmitis, and muscular disorders are prevalent among sport horses, with a fair prognosis for returning to exercise or previous performance levels. The field of equine medicine has witnessed rapid and fruitful development, resulting in a diverse range of therapeutic options for musculoskeletal problems. Staying abreast of these advancements can be challenging, prompting the need for a comprehensive review of commonly used and recent treatments. The aim is to compile current therapeutic options for managing these injuries, spanning from simple to complex physiotherapy techniques, conservative treatments including steroidal and non-steroidal anti-inflammatory drugs, hyaluronic acid, polysulfated glycosaminoglycans, pentosan polysulfate, and polyacrylamides, to promising regenerative therapies such as hemoderivatives and stem cell-based therapies. Each therapeutic modality is scrutinized for its benefits, limitations, and potential synergistic actions to facilitate their most effective application for the intended healing/regeneration of the injured tissue/organ and subsequent patient recovery. While stem cell-based therapies have emerged as particularly promising for equine musculoskeletal injuries, a multidisciplinary approach is underscored throughout the discussion, emphasizing the importance of considering various therapeutic modalities in tandem. [ABSTRACT FROM AUTHOR] more...
- Published
- 2024
- Full Text
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4. Animal models in peripheral nerve transection studies: a systematic review on study design and outcomes assessment.
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Lopes, Bruna, Coelho, André, Alvites, Rui, Sousa, Ana Catarina, Sousa, Patrícia, Moreira, Alícia, Atayde, Luís, Salgado, António, Geuna, Stefano, and Maurício, Ana Colette
- Abstract
Aim: Peripheral nerve injury regeneration studies using animal models are crucial to different pre-clinical therapeutic approaches efficacy evaluation whatever the surgical technique explored. Materials & methods: A 944 articles systematic review on 'peripheral nerve injury in animal models' over the last 9 years was carried out. Results: It was found that 91% used rodents, and only 9% employed large animals. Different nerves are studied, with generated gaps (10,78 mm) and methods applied for regeneration evaluation uniformed. Sciatic nerve was the most used (88%), followed by median and facial nerves (2.6%), significantly different. Conclusion: There has not been a significant scale-up of the in vivo testing to large animal models (anatomically/physiologically closer to humans), allowing an improvement in translational medicine for clinical cases. [ABSTRACT FROM AUTHOR] more...
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- 2024
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5. Treatment of Equine Tarsus Long Medial Collateral Ligament Desmitis with Allogenic Synovial Membrane Mesenchymal Stem/Stromal Cells Enhanced by Umbilical Cord Mesenchymal Stem/Stromal Cell-Derived Conditioned Medium: Proof of Concept.
- Author
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Leal Reis, Inês, Lopes, Bruna, Sousa, Patrícia, Sousa, Ana Catarina, Branquinho, Mariana V., Caseiro, Ana Rita, Rêma, Alexandra, Briote, Inês, Mendonça, Carla M., Santos, Jorge Miguel, Atayde, Luís M., Alvites, Rui D., and Maurício, Ana Colette more...
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SYNOVIAL membranes ,COLLATERAL ligament ,STROMAL cells ,UMBILICAL cord ,SPORTS injuries ,UMBILICAL arteries - Abstract
Simple Summary: Musculoskeletal injuries in sport horses are relatively common and quite worrisome. Tendon and ligament injuries in sport horses usually result in a long period of time out of competition. Their healing usually results in tissue fibrosis and concomitant loss of elasticity, which, depending on the severity, might prevent the horse's recovery to the same performance levels or even to athletic levels. The continuous development of regenerative medicine offers therapeutical promise. Synovial membrane mesenchymal stem/stromal cells (SM-MSCs) and umbilical cord mesenchymal stem/stromal cells (UC-MSC), as well as their growth factors, have been described as having optimal characteristics for tendon and ligament regeneration. Therefore, a therapeutical combination of SM-MSC and a conditioned medium of UC-MSC was developed, produced, and administered on a tarsal long medial collateral ligament desmitis of a show-jumping horse. The production and application of the orthobiologic therapeutical combination as well as the clinical outcome are presented herein. Horses are high-performance athletes prone to sportive injuries such as tendonitis and desmitis. The formation of fibrous tissue in tendon repair remains a challenge to overcome. This impels regenerative medicine to develop innovative therapies that enhance regeneration, retrieving original tissue properties. Multipotent Mesenchymal Stem/Stromal Cells (MSCs) have been successfully used to develop therapeutic products, as they secrete a variety of bioactive molecules that play a pivotal role in tissue regeneration. These factors are released in culture media for producing a conditioned medium (CM). The aforementioned assumptions led to the formulation of equine synovial membrane MSCs (eSM-MSCs)—the cellular pool that naturally regenerates joint tissue—combined with a medium enriched in immunomodulatory factors (among other bioactive factors) produced by umbilical cord stroma-derived MSCs (eUC-MSCs) that naturally contribute to suppressing the immune rejection in the maternal–fetal barrier. A description of an equine sport horse diagnosed with acute tarsocrural desmitis and treated with this formulation is presented. Ultrasonographic ligament recovery occurred in a reduced time frame, reducing stoppage time and allowing for the horse's return to unrestricted competition after the completion of a physical rehabilitation program. This study focused on the description of the therapeutic formulation and potential in an equine desmitis treatment using the cells themselves and their secretomes. [ABSTRACT FROM AUTHOR] more...
- Published
- 2024
- Full Text
- View/download PDF
6. Allogenic synovia-derived mesenchymal stem cells for treatment of equine tendinopathies and desmopathies — proof of concept
- Author
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Reis, Inês Leal, Lopes, Bruna, Sousa, Patrícia, Sousa, Ana Catarina, Branquinho, Mariana, Caseiro, Ana Rita, Pedrosa, Sílvia Santos, Rêma, Alexandra, Oliveira, Cláudia, Porto, Beatriz, Atayde, Luís, Amorim, Irina, Alvites, Rui, Santos, Jorge Miguel, Maurício, Ana Colette, and Veritati - Repositório Institucional da Universidade Católica Portuguesa more...
- Subjects
Synovial mesenchymal stem cell ,Clinical trials ,Cell-based therapies ,Mesenchymal stem cells ,Allogenic ,Ligament ,Tendon ,Sport horses - Abstract
Tendon and ligament injuries are frequent in sport horses and humans, and such injuries represent a significant therapeutic challenge. Tissue regeneration and function recovery are the paramount goals of tendon and ligament lesion management. Nowadays, several regenerative treatments are being developed, based on the use of stem cell and stem cell-based therapies. In the present study, the preparation of equine synovial membrane mesenchymal stem cells (eSM-MSCs) is described for clinical use, collection, transport, isolation, differentiation, characterization, and application. These cells are fibroblast-like and grow in clusters. They retain osteogenic, chondrogenic, and adipogenic differentiation potential. We present 16 clinical cases of tendonitis and desmitis, treated with allogenic eSM-MSCs and autologous serum, and we also include their evaluation, treatment, and follow-up. The concerns associated with the use of autologous serum as a vehicle are related to a reduced immunogenic response after the administration of this therapeutic combination, as well as the pro-regenerative effects from the growth factors and immunoglobulins that are part of its constitution. Most of the cases (14/16) healed in 30 days and presented good outcomes. Treatment of tendon and ligament lesions with a mixture of eSM-MSCs and autologous serum appears to be a promising clinical option for this category of lesions in equine patients. more...
- Published
- 2023
7. Ultrasound Landmarks in the Approach to the Common Peroneal Nerve in a Sheep Model—Application in Peripheral Nerve Regeneration.
- Author
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Alvites, Rui, Lopes, Bruna, Sousa, Patrícia, Sousa, Ana Catarina, Coelho, André, Moreira, Alícia, Rêma, Alexandra, Atayde, Luís, Mendonça, Carla, Luís, Ana Lúcia, and Maurício, Ana Colette
- Subjects
PERONEAL nerve ,NERVOUS system regeneration ,TIBIAL nerve ,PERIPHERAL nerve injuries ,PERIPHERAL nervous system ,SCIATIC nerve ,ULTRASONIC imaging - Abstract
Peripheral nerve injury (PNI) remains a medical challenge with no easy resolution. Over the last few decades, significant advances have been achieved in promoting peripheral nerve regeneration, and new assessment tools have been developed, both functional and imaging, to quantify the proportion and quality of nerve recovery. The exploration of new animal models, larger, more complex, and with more similarities to humans, has made it possible to reduce the gap between the results obtained in classic animal models, such as rodents, and the application of new therapies in humans and animals of clinical interest. Ultrasonography is an imaging technique recurrently used in clinical practice to assess the peripheral nerves, allowing for its anatomical and topographic characterization, aiding in the administration of anesthesia, and in the performance of nerve blocks. The use of this technique in animal models is scarce, but it could be a useful tool in monitoring the progression of nerve regeneration after the induction of controlled experimental lesions. In this work, sheep, a promising animal model in the area of peripheral nerve regeneration, were subjected to an ultrasonographic study of three peripheral nerves of the hind limb, the common peroneal, and tibial and sciatic nerves. The main aim was to establish values of dimensions and ultrasound appearance in healthy nerves and landmarks for their identification, as well as to perform an ultrasound evaluation of the cranial tibial muscle, an effector muscle of the common peroneal nerve, and to establish normal values for its ultrasound appearance and dimensions. The results obtained will allow the use of these data as control values in future work exploring new therapeutic options for nerve regeneration after induction of common peroneal nerve injuries in sheep. [ABSTRACT FROM AUTHOR] more...
- Published
- 2023
- Full Text
- View/download PDF
8. Application of Bonelike® as synthetic bone graft in orthopaedic and oral surgery in veterinary clinical cases
- Author
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Campos, José Miguel, Sousa, Ana Catarina, Pinto, Pedro Olivério, Ribeiro, Jorge, França, Miguel Lacueva, Caseiro, Ana Rita, Branquinho, Mariana Vieira, Pedrosa, Sílvia Santos, Mendonça, Carla, Brandão, Ana, Santos, José Domingos, Afonso, Américo, Atayde, Luís Miguel, Luís, Ana Lúcia, and Maurício, Ana Colette more...
- Published
- 2018
- Full Text
- View/download PDF
9. Allogenic Synovia-Derived Mesenchymal Stem Cells for Treatment of Equine Tendinopathies and Desmopathies—Proof of Concept.
- Author
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Leal Reis, Inês, Lopes, Bruna, Sousa, Patrícia, Sousa, Ana Catarina, Branquinho, Mariana, Caseiro, Ana Rita, Pedrosa, Sílvia Santos, Rêma, Alexandra, Oliveira, Cláudia, Porto, Beatriz, Atayde, Luís, Amorim, Irina, Alvites, Rui, Santos, Jorge Miguel, and Maurício, Ana Colette more...
- Subjects
MESENCHYMAL stem cells ,STEM cell treatment ,REGENERATIVE medicine ,TENDINOPATHY ,LIGAMENT injuries ,SYNOVIAL membranes ,FROZEN semen ,ENDOCHONDRAL ossification - Abstract
Simple Summary: Horses are high-level athletic athletes prone to musculoskeletal injuries. Tendon/ligament injuries are the most frequent types of injuries which that are very difficult to treat. Instead of tissue regeneration, usually, fibrous scar tissue develops which leads to decreased functionality of the injured area and threatens the participation of sport horses. The aim of regenerative medicine is to find a treatment that promotes tissue regeneration and that allows the equine patient to return to the same level of athletic performance in the shortest time period possible. In this study, we developed a solution of equine synovial membrane stem cells and autologous serum, to be injected at the lesion site to promote tissue regeneration. We describe the processes of tissue collection, preparation, isolation of synovial stem cells, expansion, culture, cryopreservation, and posterior preparation with autologous serum. The solution was tested in 16 tendons and ligaments of equines. After treatment, all equine patients underwent a physical rehabilitation program and were monitored with physical and ultrasonographic exams. The results were very promising, and thus, support the use of equine synovial stem cells and autologous serum in the treatment of tendonitis and desmitis. Tendon and ligament injuries are frequent in sport horses and humans, and such injuries represent a significant therapeutic challenge. Tissue regeneration and function recovery are the paramount goals of tendon and ligament lesion management. Nowadays, several regenerative treatments are being developed, based on the use of stem cell and stem cell-based therapies. In the present study, the preparation of equine synovial membrane mesenchymal stem cells (eSM-MSCs) is described for clinical use, collection, transport, isolation, differentiation, characterization, and application. These cells are fibroblast-like and grow in clusters. They retain osteogenic, chondrogenic, and adipogenic differentiation potential. We present 16 clinical cases of tendonitis and desmitis, treated with allogenic eSM-MSCs and autologous serum, and we also include their evaluation, treatment, and follow-up. The concerns associated with the use of autologous serum as a vehicle are related to a reduced immunogenic response after the administration of this therapeutic combination, as well as the pro-regenerative effects from the growth factors and immunoglobulins that are part of its constitution. Most of the cases (14/16) healed in 30 days and presented good outcomes. Treatment of tendon and ligament lesions with a mixture of eSM-MSCs and autologous serum appears to be a promising clinical option for this category of lesions in equine patients. [ABSTRACT FROM AUTHOR] more...
- Published
- 2023
- Full Text
- View/download PDF
10. Can $f(Q)$ gravity challenge $\Lambda$CDM?
- Author
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Atayde, Luís and Frusciante, Noemi
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Astrophysics::Cosmology and Extragalactic Astrophysics ,General Relativity and Quantum Cosmology ,Astrophysics - Cosmology and Nongalactic Astrophysics - Abstract
We study observational constraints on the non-metricity $f(Q)$-gravity which reproduces an exact $\Lambda$CDM background expansion history while modifying the evolution of linear perturbations. To this purpose we use Cosmic Microwave Background (CMB) radiation, baryonic acoustic oscillations (BAO), redshift-space distortions (RSD), supernovae type Ia (SNIa), galaxy clustering (GC) and weak gravitational lensing (WL) measurements. We set stringent constraints on the parameter of the model controlling the modifications to the gravitational interaction at linear perturbation level. We find the model to be statistically preferred by data over the $\Lambda$CDM according to the $\chi^2$ and deviance information criterion statistics for the combination with CMB, BAO, RSD and SNIa. This is mostly associated to a better fit to the low-$\ell$ tail of CMB temperature anisotropies., Comment: 5 pages, 1 figure, 2 tables, accepted for publication in PRD more...
- Published
- 2021
11. Assessment of 3D-Printed Polycaprolactone, Hydroxyapatite Nanoparticles and Diacrylate Poly(ethylene glycol) Scaffolds for Bone Regeneration.
- Author
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Sousa, Ana Catarina, Biscaia, Sara, Alvites, Rui, Branquinho, Mariana, Lopes, Bruna, Sousa, Patrícia, Valente, Joana, Franco, Margarida, Santos, José Domingos, Mendonça, Carla, Atayde, Luís, Alves, Nuno, and Maurício, Ana Colette more...
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BONE regeneration ,ETHYLENE glycol ,POLYCAPROLACTONE ,HYDROXYAPATITE ,BONE substitutes ,DENTAL pulp - Abstract
Notwithstanding the advances achieved in the last decades in the field of synthetic bone substitutes, the development of biodegradable 3D-printed scaffolds with ideal mechanical and biological properties remains an unattained challenge. In the present work, a new approach to produce synthetic bone grafts that mimic complex bone structure is explored. For the first time, three scaffolds of various composition, namely polycaprolactone (PCL), PCL/hydroxyapatite nanoparticles (HANp) and PCL/HANp/diacrylate poly(ethylene glycol) (PEGDA), were manufactured by extrusion. Following the production and characterisation of the scaffolds, an in vitro evaluation was carried out using human dental pulp stem/stromal cells (hDPSCs). Through the findings, it was possible to conclude that, in all groups, the scaffolds were successfully produced presenting networks of interconnected channels, adequate porosity for migration and proliferation of osteoblasts (approximately 50%). Furthermore, according to the in vitro analysis, all groups were considered non-cytotoxic in contact with the cells. Nevertheless, the group with PEGDA revealed hydrophilic properties (15.15° ± 4.06) and adequate mechanical performance (10.41 MPa ± 0.934) and demonstrated significantly higher cell viability than the other groups analysed. The scaffolds with PEGDA suggested an increase in cell adhesion and proliferation, thus are more appropriate for bone regeneration. To conclude, findings in this study demonstrated that PCL, HANp and PEGDA scaffolds may have promising effects on bone regeneration and might open new insights for 3D tissue substitutes. [ABSTRACT FROM AUTHOR] more...
- Published
- 2022
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12. The Application of Mesenchymal Stem Cells on Wound Repair and Regeneration.
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Lopes, Bruna, Sousa, Patrícia, Alvites, Rui, Branquinho, Mariana, Sousa, Ana, Mendonça, Carla, Atayde, Luís Miguel, Maurício, Ana Colette, and Klar, Agnes
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WOUND healing ,MESENCHYMAL stem cells ,TISSUE scaffolds ,REGENERATION (Biology) ,SKIN regeneration ,STEM cells - Abstract
In the past decades, regenerative medicine applied on skin lesions has been a field of constant improvement for both human and veterinary medicine. The process of healing cutaneous wound injuries implicates a well-organized cascade of molecular and biological processes. However, sometimes the normal process fails and can result in a chronic lesion. In addition, wounds are considered an increasing clinical impairment, due to the progressive ageing of the population, as well as the prevalence of concomitant diseases, such as diabetes and obesity, that represent risk-aggravating factors for the development of chronic skin lesions. Stem cells' regenerative potential has been recognized worldwide, including towards skin lesion repair, Tissue engineering techniques have long been successfully associated with stem cell therapies, namely the application of three-dimensional (3D) bioprinted scaffolds. With this review, we intend to explore several stem cell sources with promising aptitude towards skin regeneration, as well as different techniques used to deliver those cells and provide a supporting extracellular matrix environment, with effective outcomes. Furthermore, different studies are discussed, both in vitro and in vivo, in terms of their relevance in the skin regeneration field. [ABSTRACT FROM AUTHOR] more...
- Published
- 2021
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- View/download PDF
13. Peripheral Nerve Injury Treatments and Advances: One Health Perspective.
- Author
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Lopes, Bruna, Sousa, Patrícia, Alvites, Rui, Branquinho, Mariana, Sousa, Ana Catarina, Mendonça, Carla, Atayde, Luís Miguel, Luís, Ana Lúcia, Varejão, Artur S. P., and Maurício, Ana Colette
- Subjects
PERIPHERAL nerve injuries ,DISABILITIES ,DIABETIC neuropathies ,PERIPHERAL nervous system ,NERVOUS system regeneration ,NEURALGIA - Abstract
Peripheral nerve injuries (PNI) can have several etiologies, such as trauma and iatrogenic interventions, that can lead to the loss of structure and/or function impairment. These changes can cause partial or complete loss of motor and sensory functions, physical disability, and neuropathic pain, which in turn can affect the quality of life. This review aims to revisit the concepts associated with the PNI and the anatomy of the peripheral nerve is detailed to explain the different types of injury. Then, some of the available therapeutic strategies are explained, including surgical methods, pharmacological therapies, and the use of cell-based therapies alone or in combination with biomaterials in the form of tube guides. Nevertheless, even with the various available treatments, it is difficult to achieve a perfect outcome with complete functional recovery. This review aims to enhance the importance of new therapies, especially in severe lesions, to overcome limitations and achieve better outcomes. The urge for new approaches and the understanding of the different methods to evaluate nerve regeneration is fundamental from a One Health perspective. In vitro models followed by in vivo models are very important to be able to translate the achievements to human medicine. [ABSTRACT FROM AUTHOR] more...
- Published
- 2022
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14. Small Ruminants and Its Use in Regenerative Medicine: Recent Works and Future Perspectives.
- Author
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Alvites, Rui Damásio, Branquinho, Mariana Vieira, Sousa, Ana Catarina, Lopes, Bruna, Sousa, Patrícia, Mendonça, Carla, Atayde, Luís Miguel, Maurício, Ana Colette, and Dias, Isabel R.
- Subjects
REGENERATIVE medicine ,RUMINANTS ,SCIENTIFIC literature ,ANIMAL models in research ,LABORATORY animals ,TRANSLATIONAL research ,GASTROINTESTINAL system - Abstract
Simple Summary: Small ruminants such as sheep and goats have been increasingly used as animal models due to their dimensions, physiology and anatomy identical to those of humans. Their low costs, ease of accommodation, great longevity and easy handling make them advantageous animals to be used in a wide range of research work. Although there is already a lot of scientific literature describing these species, their use still lacks some standardization. The purpose of this review is to summarize the general principles related to the use of small ruminants as animal models for scientific research. Medical and translational scientific research requires the use of animal models as an initial approach to the study of new therapies and treatments, but when the objective is an exploration of translational potentialities, classical models fail to adequately mimic problems in humans. Among the larger animal models that have been explored more intensely in recent decades, small ruminants, namely sheep and goats, have emerged as excellent options. The main advantages associated to the use of these animals in research works are related to their anatomy and dimensions, larger than conventional laboratory animals, but very similar to those of humans in most physiological systems, in addition to their low maintenance and feeding costs, tendency to be docile, long life expectancies and few ethical complications raised in society. The most obvious disadvantages are the significant differences in some systems such as the gastrointestinal, and the reduced amount of data that limits the comparison between works and the validation of the characterization essays. Despite everything, recently these species have been increasingly used as animal models for diseases in different systems, and the results obtained open doors for their more frequent and advantageous use in the future. The purpose of this review is to summarize the general principles related to the use of small ruminants as animal models, with a focus on regenerative medicine, to group the most relevant works and results published recently and to highlight the potentials for the near future in medical research. [ABSTRACT FROM AUTHOR] more...
- Published
- 2021
- Full Text
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15. Establishment of a Sheep Model for Hind Limb Peripheral Nerve Injury: Common Peroneal Nerve.
- Author
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D. Alvites, Rui, V. Branquinho, Mariana, Sousa, Ana C., Zen, Federica, Maurina, Monica, Raimondo, Stefania, Mendonça, Carla, Atayde, Luís, Geuna, Stefano, Varejão, Artur S.P., Maurício, Ana C., and Tonazzini, Ilaria more...
- Subjects
PERONEAL nerve ,PERIPHERAL nervous system ,HINDLIMB ,DRUG efficacy ,SHEEP ,SHEEP diseases - Abstract
Thousands of people worldwide suffer from peripheral nerve injuries and must deal daily with the resulting physiological and functional deficits. Recent advances in this field are still insufficient to guarantee adequate outcomes, and the development of new and compelling therapeutic options require the use of valid preclinical models that effectively replicate the characteristics and challenges associated with these injuries in humans. In this study, we established a sheep model for common peroneal nerve injuries that can be applied in preclinical research with the advantages associated with the use of large animal models. The anatomy of the common peroneal nerve and topographically related nerves, the functional consequences of its injury and a neurological examination directed at this nerve have been described. Furthermore, the surgical protocol for accessing the common peroneal nerve, the induction of different types of nerve damage and the application of possible therapeutic options were described. Finally, a preliminary morphological and stereological study was carried out to establish control values for the healthy common peroneal nerves regarding this animal model and to identify preliminary differences between therapeutic methods. This study allowed to define the described lateral incision as the best to access the common peroneal nerve, besides establishing 12 and 24 weeks as the minimum periods to study lesions of axonotmesis and neurotmesis, respectively, in this specie. The post-mortem evaluation of the harvested nerves allowed to register stereological values for healthy common peroneal nerves to be used as controls in future studies, and to establish preliminary values associated with the therapeutic performance of the different applied options, although limited by a small sample size, thus requiring further validation studies. Finally, this study demonstrated that the sheep is a valid model of peripheral nerve injury to be used in pre-clinical and translational works and to evaluate the efficacy and safety of nerve injury therapeutic options before its clinical application in humans and veterinary patients. [ABSTRACT FROM AUTHOR] more...
- Published
- 2021
- Full Text
- View/download PDF
16. Can f(Q) gravity challenge ΛCDM?
- Author
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Atayde, Luís and Frusciante, Noemi
- Subjects
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GRAVITATIONAL interactions , *TYPE I supernovae , *GRAVITY , *COSMIC background radiation , *GALAXY clusters , *GRAVITATIONAL lenses , *RADIATION - Abstract
We study observational constraints on the nonmetricity f(Q)-gravity which reproduces an exact ΛCDM background expansion history while modifying the evolution of linear perturbations. To this purpose we use cosmic microwave background (CMB) radiation, baryonic acoustic oscillations (BAO), redshift-space distortions (RSD), supernovae type Ia (SNIa), galaxy clustering (GC) and weak gravitational lensing (WL) measurements. We set stringent constraints on the parameter of the model controlling the modifications to the gravitational interaction at linear perturbation level. We find the model to be statistically preferred by data over the ΛCDM according to the χ² and deviance information criterion statistics for the combination with CMB, BAO, RSD and SNIa. This is mostly associated to a better fit to the low-ℓ tail of CMB temperature anisotropies. [ABSTRACT FROM AUTHOR] more...
- Published
- 2021
- Full Text
- View/download PDF
17. Phenomenology of the generalized cubic covariant Galileon model and cosmological bounds.
- Author
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Frusciante, Noemi, Peirone, Simone, Atayde, Luís, and De Felice, Antonio
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COSMIC background radiation , *DARK energy , *TYPE I supernovae , *HUBBLE constant , *NEUTRINOS , *NEUTRINO mass , *PHYSICAL cosmology , *CEPHEIDS - Abstract
We investigate the generalized cubic covariant Galileon model, a kinetically driven dark energy model within the Horndeski class of theories. The model extends the cubic covariant Galileon by including power laws of the field derivatives in the K-essence and cubic terms which still allow for tracker solutions. We study the shape of the viable parameter space by enforcing stability conditions which include the absence of ghost, gradient and tachyon instabilities and the avoidance of strong coupling at early time. We study here the relevant effects of the modifications induced by the model on some cosmological observables such as the cosmic microwave background (CMB), the lensing potential autocorrelation and the matter power spectrum. For this goal, we perform parameter estimation using data of CMB temperature and polarization, baryonic acoustic oscillations (BAO), redshift-space distortions (RSD), supernovae type Ia (SNIa) and Cepheids. Data analysis with CMB alone finds that the today's Hubble parameter H0 is consistent with its determination from Cepheids at 1σ, resolving the famous tension of the cosmological standard models. The joint analysis of CMB, BAO, RSD and SNIa sets a lower bound for the sum of neutrino masses which is Σmν>0.11 eV at 1σ, in addition to the usual upper limit. The model selection analysis based on the effective χeff² and deviance information criterion is not able to clearly identify the statistically favored model between ΛCDM and the generalized cubic covariant Galileon, from which we conclude that the latter model deserves further studies. [ABSTRACT FROM AUTHOR] more...
- Published
- 2020
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18. Treatment of Equine Tarsus Long Medial Collateral Ligament Desmitis with Allogenic Synovial Membrane Mesenchymal Stem/Stromal Cells Enhanced by Umbilical Cord Mesenchymal Stem/Stromal Cell-Derived Conditioned Medium: Proof of Concept.
- Author
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Leal Reis I, Lopes B, Sousa P, Sousa AC, Branquinho MV, Caseiro AR, Rêma A, Briote I, Mendonça CM, Santos JM, Atayde LM, Alvites RD, and Maurício AC
- Abstract
Horses are high-performance athletes prone to sportive injuries such as tendonitis and desmitis. The formation of fibrous tissue in tendon repair remains a challenge to overcome. This impels regenerative medicine to develop innovative therapies that enhance regeneration, retrieving original tissue properties. Multipotent Mesenchymal Stem/Stromal Cells (MSCs) have been successfully used to develop therapeutic products, as they secrete a variety of bioactive molecules that play a pivotal role in tissue regeneration. These factors are released in culture media for producing a conditioned medium (CM). The aforementioned assumptions led to the formulation of equine synovial membrane MSCs (eSM-MSCs)-the cellular pool that naturally regenerates joint tissue-combined with a medium enriched in immunomodulatory factors (among other bioactive factors) produced by umbilical cord stroma-derived MSCs (eUC-MSCs) that naturally contribute to suppressing the immune rejection in the maternal-fetal barrier. A description of an equine sport horse diagnosed with acute tarsocrural desmitis and treated with this formulation is presented. Ultrasonographic ligament recovery occurred in a reduced time frame, reducing stoppage time and allowing for the horse's return to unrestricted competition after the completion of a physical rehabilitation program. This study focused on the description of the therapeutic formulation and potential in an equine desmitis treatment using the cells themselves and their secretomes. more...
- Published
- 2024
- Full Text
- View/download PDF
19. Allogenic Synovia-Derived Mesenchymal Stem Cells for Treatment of Equine Tendinopathies and Desmopathies-Proof of Concept.
- Author
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Leal Reis I, Lopes B, Sousa P, Sousa AC, Branquinho M, Caseiro AR, Pedrosa SS, Rêma A, Oliveira C, Porto B, Atayde L, Amorim I, Alvites R, Santos JM, and Maurício AC
- Abstract
Tendon and ligament injuries are frequent in sport horses and humans, and such injuries represent a significant therapeutic challenge. Tissue regeneration and function recovery are the paramount goals of tendon and ligament lesion management. Nowadays, several regenerative treatments are being developed, based on the use of stem cell and stem cell-based therapies. In the present study, the preparation of equine synovial membrane mesenchymal stem cells (eSM-MSCs) is described for clinical use, collection, transport, isolation, differentiation, characterization, and application. These cells are fibroblast-like and grow in clusters. They retain osteogenic, chondrogenic, and adipogenic differentiation potential. We present 16 clinical cases of tendonitis and desmitis, treated with allogenic eSM-MSCs and autologous serum, and we also include their evaluation, treatment, and follow-up. The concerns associated with the use of autologous serum as a vehicle are related to a reduced immunogenic response after the administration of this therapeutic combination, as well as the pro-regenerative effects from the growth factors and immunoglobulins that are part of its constitution. Most of the cases (14/16) healed in 30 days and presented good outcomes. Treatment of tendon and ligament lesions with a mixture of eSM-MSCs and autologous serum appears to be a promising clinical option for this category of lesions in equine patients. more...
- Published
- 2023
- Full Text
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