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The seismic responses of a single-story reinforced concrete (RC) frame building model under control using an active mass damper (AMD) are demonstrated through a real-time hybrid simulation (RTHS) method. In this study, the RTHS test is carried out by using a hydraulic actuator and a shaking table under synchronization. Most parts of the target RC frame model are provided as an analytical model for an online computer simulation, and only the single column of the first story is prepared as an experimental substructure. A hydraulic actuator deforms the actual RC column, and uncertainty or nonlinearity of the RC column's behavior is focused on in this RTHS test. At the same time, a control device of AMD is actually tested under a situation of installing it on the target building's floor. The floor response of the target building model is generated using a shaking table. A control motion of the AMD is manipulated based on an online simulation of the entire RC building model. Firstly, a time delay compensation of the hydraulic actuator is considered. Time delay parameters are identified using a combination model of a time lag and a first-order delay. A PID controller and a time series compensator are applied to improve actuator performances. Next, the reproducibility of the RTHS test using two-individual actuators is evaluated. The tracking of a restoring force and deformation of the actual RC column specimen generated by the hydraulic actuator and floor motion responses reproduced on the shaking table are investigated. To improve the online numerical simulation based on the measured force responses of the RC column specimen, a high-pass filter (HPF) is applied for a force correction to utilize its phase-lead property. The effect of this HPF force correction is evaluated in both a linear region and a strong nonlinear region of the actual RC column specimen. Finally, the RTHS test results are compared to fully numerical simulations and the control effect of the AMD to increase the damping effect for the target RC building model is also investigated.

Purpose: We quantified the inter-fractional changes associated with passive carbon-ion radiotherapy using vertical and horizontal beam fields for prostate cancer. Methods: In total, 118 treatment-room computed tomography (TRCT) image sets were acquired from 10 patients. Vertical (anterior–posterior) and horizontal (left–right) fields were generated on the planning target volume identified by treatment planning CT. The dose distribution for each field was recalculated on each TRCT image set at the bone-matching position and evaluated using the dose–volume parameters for the prostate and rectum V95 values. To confirm adequate margins, we generated vertical and horizontal fields with 0-, 2-, 4-, and 6-mm isotropic margins from the prostate and recalculated the dose distributions on all TRCT image sets. Sigmoid functions were fitted to a plot of acceptable ratios (that is, when prostate V95 > 98%) vs. the isotropic margin size to identify the margin at which this ratio was achieved in 95% of patients with a vertical or horizontal field. Results: The prostate V95 values (mean ± standard deviation) were 99.89 ± 0.62% and 99.99 ± 0.00% with vertical and horizontal fields, respectively; this difference was not statistically significant (p = 0.067). The rectum V95 values were 1.93 ± 1.25 and 1.88 ± 0.96 ml with vertical and horizontal fields, respectively; the difference was not statistically significant (p = 0.432). The estimated adequate margins were 2.2 and 3.0 mm for vertical and horizontal fields, respectively. Conclusions: Although there is no significant difference, horizontal fields offer higher reproducibility for prostate dosing than vertical fields in our clinical setting, and 3.0 mm was found to be an adequate margin for inter-fractional changes.

Both IgA and IgG antibodies are known to play important roles in protection against influenza virus infection. While IgG is the major isotype induced systemically, IgA is predominant in mucosal tissues, including the upper respiratory tract. Although IgA antibodies are believed to have unique advantages in mucosal immunity, information on direct comparisons of the in vitro antiviral activities of IgA and IgG antibodies recognizing the same epitope is limited. In this study, we demonstrate differences in antiviral activities between these isotypes using monoclonal IgA and IgG antibodies obtained from hybridomas of the same origin. Polymeric IgA-producing hybridoma cells were successfully subcloned from those originally producing monoclonal antibody S139/1, a hemaggulutinin (HA)-specific IgG that was generated against an influenza A virus strain of the H3 subtype but had cross-neutralizing activities against the H1, H2, H13, and H16 subtypes. These monoclonal S139/1 IgA and IgG antibodies were assumed to recognize the same epitope and thus used to compare their antiviral activities. We found that both S139/1 IgA and IgG antibodies strongly bound to the homologous H3 virus in an enzyme-linked immunosorbent assay, and there were no significant differences in their hemagglutination-inhibiting and neutralizing activities against the H3 virus. In contrast, S139/1 IgA showed remarkably higher cross-binding to and antiviral activities against H1, H2, and H13 viruses than S139/1 IgG. It was also noted that S139/1 IgA, but not IgG, drastically suppressed the extracellular release of the viruses from infected cells. Electron microscopy revealed that S139/1 IgA deposited newly produced viral particles on the cell surface, most likely by tethering the particles. These results suggest that anti-HA IgA has greater potential to prevent influenza A virus infection than IgG antibodies, likely due to increased avidity conferred by its multivalency, and that this advantage may be particularly important for heterosubtypic immunity.