195 results on '"Bagagli, Eduardo"'
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2. Molecular detection of Paracoccidioides spp. in environmental samples from the Southwestern Amazon
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Ferreira, Iasminy R. Silva, de M. Teixeira, Marcus, de Araújo, Atilon V., Bagagli, Eduardo, Garces, Hans G., Peters, Leila P., and Carvalho, Clarice M.
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- 2024
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3. PRP8 Intein in Onygenales: Distribution and Phylogenetic Aspects
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Garcia Garces, Hans, Hamae Yamauchi, Danielle, Theodoro, Raquel Cordeiro, and Bagagli, Eduardo
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- 2020
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4. Infections Caused by Fusarium Species in Pediatric Cancer Patients and Review of Published Literature
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Arnoni, Mariana Volpe, Paula, Claudete Rodrigues, Auler, Marcos Ereno, Simões, Cirilo Cesar Naozuka, Nakano, Shirley, Szeszs, Maria Walderez, Melhem, Márcia de Souza Carvalho, Pereira, Virgínia Bodelão Richini, Garces, Hans Garcia, Bagagli, Eduardo, Silva, Eriques Gonçalves, de Macêdo, Melissa Ferreira, and Ruiz, Luciana da Silva
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- 2018
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5. A taxonomic review of the genus Paracoccidioides, with focus on the uncultivable species.
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Vilela, Raquel, de Hoog, Sybren, Bensch, Konstanze, Bagagli, Eduardo, and Mendoza, Leonel
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SPECIES ,PARACOCCIDIOIDOMYCOSIS ,SKIN diseases ,CETACEA ,DOLPHINS - Abstract
Paracoccidioides species have always been surrounded by taxonomic uncertainties. The continuing nomenclatoral muddle was caused in part by the failure of Adolfo Lutz and Jorge Lôbo to name the etiologic agents of human paracoccidioidomycosis and Jorge Lôbo's diseases, respectively. Early in their history, it was postulated that the cultivable species causing systemic infections belonged in the genus Paracoccidioides, whereas the uncultivable species, causing skin disease, were not part of the genus. The taxonomy of these pathogens was further complicated when a similar skin disease with numerous yeast-like cells in infected dolphins was also reported. Due to its phenotypic similarities with that described by Jorge Lôbo in human and its uncultivable nature, it was assumed that the disease in dolphins was caused by the same fungus. Recent molecular and population genetic analysis, however, found the DNA extracted from the uncultivable yeast-like cells affecting dolphins shared common phylogenetic traits with cultivable Paracoccidioides species. The study revealed that the uncultivable pathogens comprised 2 different Paracoccidioides species, now known as P. ceti and P. loboi, correspondingly. To validate P. loboi binomial, a comprehensive historical critical review of Jorge Lôbo etiology was performed. This review showed the proposed binomial P. loboi was previously used, and, thus, a replacement name is introduced, Paracoccidioides lobogeorgii nom. nov. In addition, in this review, several cultivable human Paracoccidioides species are validated, and the generic type species, P. brasiliensis, is neotypified as the original material could not be traced. [ABSTRACT FROM AUTHOR]
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- 2023
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6. PESQUISA DE ASPERGILLUS SPP EM AMBIENTE HOSPITALAR: DADOS PRELIMINARES
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Cardoso, Luiza Ikeda Seixas, Bagagli, Eduardo, Mendes, Rinaldo Poncio, and Cavalcante, Ricardo de Souza
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- 2022
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7. Molecular Phylogenetic Analysis of Paracoccidioides Species Complex Present in Paracoccidioidomycosis Patient Tissue Samples.
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de Oliveira, Luciana Bonome Zeminian, Della Coletta, Amanda Manoel, Gardizani, Taiane Priscila, Garces, Hans Garcia, Bagagli, Eduardo, Trilles, Luciana, Barrozo, Ligia Vizeu, Marques, Sílvio de Alencar, Faveri, Julio De, and Dias-Melicio, Luciane Alarcão
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PARACOCCIDIOIDOMYCOSIS ,PARACOCCIDIOIDES brasiliensis ,SPECIES ,GENETIC correlations ,MYCOSES - Abstract
Paracoccidioidomycosis (PCM) is the main and most prevalent systemic mycosis in Latin America, that until recently, it was believed to be caused only by Paracoccidioides brasiliensis (P. brasiliensis). In 2006, researchers described three cryptic species: S1, PS2, PS3, and later, another one, PS4. In 2009, Paracoccidioides lutzii (Pb01-like) was described, and in 2017, a new nomenclature was proposed for the different agents: P. brasiliensis (S1), P. americana (PS2), P. restrepiensis (PS3), and P. venezuelensis (PS4). These species are not uniformly distributed throughout Latin America and, knowing that more than one cryptic species could coexist in some regions, we aimed to identify those species in patients' biopsy samples for a better understanding of the distribution and occurrence of these recently described species in Botucatu region. The Hospital of Medical School of Botucatu—UNESP, which is a PCM study pole, is located in São Paulo State mid-west region and is classified as a PCM endemic area. Genotyping analyses of clinical specimens from these patients that have been diagnosed and treated in our Hospital could favor a possible correlation between genetic groups and mycological and clinical characteristics. For this, molecular techniques to differentiate Paracoccidioides species in these biopsies, such as DNA extraction, PCR, and sequencing of three target genes (ITS, CHS2, and ARF) were conducted. All the sequences were analyzed at BLAST to testify the presence of P. brasiliensis. The phylogenetic trees were constructed using Mega 7.0 software and showed that 100% of our positive samples were from S1 cryptic species, therefore P. brasiliensis. This is important data, demonstrating the predominance of this species in the São Paulo State region. [ABSTRACT FROM AUTHOR]
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- 2023
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8. Sporothrix schenckii sensu stricto Isolated from Soil in an Armadillo’s Burrow
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Rodrigues, Anderson Messias, Bagagli, Eduardo, de Camargo, Zoilo Pires, and de Moraes Gimenes Bosco, Sandra
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- 2014
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9. Cryptococcus neoformans Prp8 Intein: An In Vivo Target-Based Drug Screening System in Saccharomyces cerevisiae to Identify Protein Splicing Inhibitors and Explore Its Dynamics.
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Fernandes, José Alex Lourenço, Zatti, Matheus da Silva, Arantes, Thales Domingos, Souza, Maria Fernanda Bezerra de, Santoni, Mariana Marchi, Rossi, Danuza, Zanelli, Cleslei Fernando, Liu, Xiang-Qin, Bagagli, Eduardo, and Theodoro, Raquel Cordeiro
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CRYPTOCOCCUS neoformans ,MOBILE genetic elements ,SACCHAROMYCES cerevisiae ,FUNGAL proteins ,PROTEINS - Abstract
Inteins are genetic mobile elements that are inserted within protein-coding genes, which are usually housekeeping genes. They are transcribed and translated along with the host gene, then catalyze their own splicing out of the host protein, which assumes its functional conformation thereafter. As Prp8 inteins are found in several important fungal pathogens and are absent in mammals, they are considered potential therapeutic targets since inhibiting their splicing would selectively block the maturation of fungal proteins. We developed a target-based drug screening system to evaluate the splicing of Prp8 intein from the yeast pathogen Cryptococcus neoformans (CnePrp8i) using Saccharomyces cerevisiae Ura3 as a non-native host protein. In our heterologous system, intein splicing preserved the full functionality of Ura3. To validate the system for drug screening, we examined cisplatin, which has been described as an intein splicing inhibitor. By using our system, new potential protein splicing inhibitors may be identified and used, in the future, as a new class of drugs for mycosis treatment. Our system also greatly facilitates the visualization of CnePrp8i splicing dynamics in vivo. [ABSTRACT FROM AUTHOR]
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- 2022
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10. Paracoccidioidomycosis due to Paracoccidioides lutzii complicated with adrenal injury and pulmonary arterial hypertension
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Gaspar, Gilberto Gambero, Cocio, Tiago Alexandre, Nascimento, Erika, Fabro, Alexandre Todorovic, Kress, Marcia Regina von Zeska, Bagagli, Eduardo, and Martinez, Roberto
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Paracoccidioides lutzii ,Paracoccidioidomycosis ,Pulmonary arterial hypertension - Abstract
Paracoccidioidomycosis caused by Paracoccidioides lutzii is endemic in the Midwest of Brazil and its clinical spectrum is still little known due to the recent identification of this fungal species. A patient resident in Southeast Brazil, but who had lived for many years in the Midwest region, presented with skin injuries, chronic cough and bilateral adrenal involvement. Paracoccidioides spp. was isolated in culture from a skin lesion biopsy. This isolate was later identified as P. lutzii using gene sequencing. A favorable initial response to treatment with itraconazole was observed, but a few weeks later, the patient developed respiratory failure and worsening of lung lesions. Evaluation by computed tomography and echocardiography were suggestive of pulmonary arterial hypertension, and a bronchoscopic biopsy showed peribronchial remodeling. The patient completed the antifungal treatment but maintained the respiratory dysfunction. The reported case shows that P. lutzii can be isolated from patients in a geographic area far from the place of infection acquisition and that, as P. brasiliensis , it can cause adrenal injury and cardio-respiratory complications as a consequence of excessive necrosis and fibrosis.
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- 2020
11. Paracoccidioidomycosis in a Dog: Case Report of Generalized Lymphadenomegaly
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Rodrigues de Farias, Marconi, Anuska Zeni Condas, Larissa, Garcia Ribeiro, Márcio, de Moraes Gimenes Bosco, Sandra, Dominguez Muro, Marisol, Werner, Juliana, Cordeiro Theodoro, Raquel, Bagagli, Eduardo, Alencar Marques, Sílvio, and Franco, Marcello
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- 2011
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12. Search for Mycobacterium leprae in wild mammals
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Pedrini, Silvia Cristina Barboza, Rosa, Patricia Sammarco, Medri, Isis Meri, Mourao, Guilherme, Bagagli, Eduardo, and Lopes, Carlos Alberto de Magalhaes
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- 2010
13. Dimorphism, Thermal Tolerance, Virulence and Heat Shock Protein 70 Transcription in Different Isolates of Paracoccidioides brasiliensis
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Theodoro, Raquel Cordeiro, de Moraes Gimenes Bosco, Sandra, Araújo, Jr, João Pessoa, Candeias, João Manuel Grisi, da Graça Macoris, Severino Assis, Trinca, Luzia Aparecida, and Bagagli, Eduardo
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- 2008
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14. Paracoccidioides brasiliensis: phylogenetic and ecological aspects
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Bagagli, Eduardo, Theodoro, Raquel C., Bosco, Sandra M. G., and McEwen, Juan G.
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- 2008
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15. Clinical and Eco-Epidemiological Aspects of a Novel Hyperendemic Area of Paracoccidioidomycosis in the Tocantins-Araguaia Basin (Northern Brazil), Caused by Paracoccidioides sp.
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Krakhecke-Teixeira, Alessandra G., Yamauchi, Danielle H., Rossi, Alexsandra, de Sousa, Herdson R., Garces, Hans G., Júnior, Joaquim L., Júnior, Antônio O. S., Felipe, Maria Sueli S., Bagagli, Eduardo, de Andrade Jr., Heitor F., and Teixeira, Marcus de M.
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BIOLOGICAL specimens ,ENVIRONMENTAL sampling ,PUBLIC hospitals ,MYCOSES ,MALE employees - Abstract
Paracoccidioidomycosis (PCM) is the most prevalent systemic mycosis in Brazil. The disease is caused by dimorphic fungi nested within the Paracoccidioides genus. We described 106 PCM cases (47.1 cases/year) at the Tropical Diseases Public Hospital of Tocantins State. PCM was prevalent in males and rural workers over 50 years; the chronic pulmonary form predominated in 67% of cases. The male-to-female ratio was 2.65:1, with more women affected than other endemic regions of Brazil. Urban or indoor activities were reported in women and are ascribed to disease urbanization. qPCR-based assays confirmed the identification of Paracoccidioides DNA in 37 biological specimens. Paracoccidioides sp. DNA was found in 53% of the environmental samples, suggesting autochthonous infections. Therefore, the Tocantins-Araguaia basin must be considered a novel hyperendemic area of PCM in Brazil, reinforcing the importance of including PCM as a notifiable disease, requiring specific diagnosis and health measures. [ABSTRACT FROM AUTHOR]
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- 2022
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16. Outbreak of equine pythiosis in a southeastern region of Brazil: Environmental isolation and phylogeny.
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Souza da Paz, Giselle, Gasparini Camargo, Gabriel, Eduardo Cury, José, Pereira Apolonio, Emanuel Vitor, Garcia Garces, Hans, Carolina do Prado, Ana, Luana Chechi, Jessica, Lucena Oliveira, Alana, Jun Watanabe, Marcos, Bagagli, Eduardo, and Gimenes Bosco, Sandra de Moraes
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CYTOCHROME oxidase ,OZONE therapy ,PHYLOGENY ,ENVIRONMENTAL sampling ,WATER sampling ,GRAZING - Abstract
Pythiosis is a disease caused by the oomycete Pythium insidiosum, mainly reported in equines, dogs and humans and directly transmitted through contaminant zoospores in aquatic environments. We report the first outbreak of equine pythiosis in five equines. Wound samples were submitted for diagnostic testing including mycological culture and nested PCR. Treatment approaches consisted of conventional and alternative therapies. Microbiological analyses were performed using water samples from the riverbanks close to where the animals had grazed. All animals were positive for P. insidiosum cultures, and two animals responded successfully to alternative therapy (ozone therapy). After culture and molecular analysis of environmental samples, the presence of P. insidiosum in one section of the Tietê River was confirmed through a 99% sequence identity. Phylogenetic analyses using the cytochrome oxidase II gene showed that the animal isolates clustered in clade I and the environmental isolates clustered in clade III. Although the environmental and wound isolates belonged to different genetic clades, we concluded that the Tietê River is an important source of infection by P. insidiosum and that research concerning environmental isolation of P. insidiosum from rivers and lakes should be strongly facilitated in Brazil. [ABSTRACT FROM AUTHOR]
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- 2022
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17. Systemic Infection by Non- albicans Candida Species Affects the Development of a Murine Model of Multiple Sclerosis.
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Fraga-Silva, Thais Fernanda de Campos, Munhoz-Alves, Natália, Mimura, Luiza Ayumi Nishiyama, Oliveira, Larissa Ragozo Cardoso de, Figueiredo-Godoi, Lívia Mara Alves, Garcia, Maíra Terra, Oliveira, Evelyn Silva, Ishikawa, Larissa Lumi Watanabe, Zorzella-Pezavento, Sofia Fernanda Gonçalves, Bonato, Vânia Luiza Deperon, Junqueira, Juliana Campos, Bagagli, Eduardo, and Sartori, Alexandrina
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CANDIDA albicans ,MULTIPLE sclerosis ,MYELIN oligodendrocyte glycoprotein ,GREATER wax moth ,MYELOID cells ,MICROGLIA ,CANDIDIASIS - Abstract
Candidiasis may affect the central nervous system (CNS), and although Candida albicans is predominant, non-albicans Candida species can also be associated with CNS infections. Some studies have suggested that Candida infections could increase the odds of multiple sclerosis (MS) development. In this context, we investigated whether systemic infection by non-albicans Candida species would affect, clinically or immunologically, the severity of experimental autoimmune encephalomyelitis (EAE), which is an animal model used to study MS. For this, a strain of C. glabrata, C. krusei, and C. parapsilosis was selected and characterized using different in vitro and in vivo models. In these analysis, all the strains exhibited the ability to form biofilms, produce proteolytic enzymes, and cause systemic infections in Galleria mellonella, with C. glabrata being the most virulent species. Next, C57BL/6 mice were infected with strains of C. glabrata, C. krusei, or C. parapsilosis, and 3 days later were immunized with myelin oligodendrocyte glycoprotein to develop EAE. Mice from EAE groups previously infected with C. glabrata and C. krusei developed more severe and more prevalent paralysis, while mice from the EAE group infected with C. parapsilosis developed a disease comparable to non-infected EAE mice. Disease aggravation by C. glabrata and C. krusei strains was concomitant to increased IL-17 and IFN-γ production by splenic cells stimulated with fungi-derived antigens and with increased percentage of T lymphocytes and myeloid cells in the CNS. Analysis of interaction with BV-2 microglial cell line also revealed differences among these strains, in which C. krusei was the strongest activator of microglia concerning the expression of MHC II and CD40 and pro-inflammatory cytokine production. Altogether, these results indicated that the three non-albicans Candida strains were similarly able to reach the CNS but distinct in terms of their effect over EAE development. Whereas C. glabrata and C. Krusei aggravated the development of EAE, C. parapsilosis did not affect its severity. Disease worsening was partially associated to virulence factors in C. glabrata and to a strong activation of microglia in C. krusei infection. In conclusion, systemic infections by non-albicans Candida strains exerted influence on the experimental autoimmune encephalomyelitis in both immunological and clinical aspects, emphasizing their possible relevance in MS development. [ABSTRACT FROM AUTHOR]
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- 2022
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18. Experimental infections with Paracoccidioides brasiliensis obtained from armadillos: comparison to clinical isolates
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Nishikaku, Angela Satie, Peracoli, Maria Terezinha S., Bagagli, Eduardo, Sugizaki, Maria Fatima, and Sartori, Alexandrina
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- 2008
19. Human Pythiosis, Brazil
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Bosco, Sandra de Moraes Gimenes, Bagagli, Eduardo, Araujo, Joao Pessoa, Jr., Candeias, Joao Manuel Grisi, de Franco, Marcello Fabiano, Marques, Mariangela Esther Alencar, Mendoza, Leonel, de Camargo, Rosangela Pires, and Marques, Silvio Alencar
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Mycoses -- Research ,Mycoses -- Case studies ,Mycoses -- Care and treatment ,Mycoses -- Diagnosis - Abstract
Pythiosis, caused by Pythium insidiosum, occurs in humans and animals and is acquired from aquatic environments that harbor the emerging pathogen. Diagnosis is difficult because clinical and histopathologic features are [...]
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- 2005
20. 290. Microbiological Evaluation of Different Strategies for Management of Snakes in Captivity
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Vanessa Campagner, M. V. Michelle, Bosco, Sandra M.G., Bagagli, Eduardo, de Lourdes R.S. Cunha, Maria, Jeronimo, Bruna C., Saad, Eduardo, Biscola, Natalia P., Ferreira-Junior, Rui Seabra, and Barraviera, Benedito
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- 2012
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21. Comparison between human and armadillo Paracoccidioides brasiliensis isolates by random amplified polymorphic DNA analysis
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Sano, Ayako, Tanaka, Reiko, Yokoyama, Koji, Franco, Marcello, Bagagli, Eduardo, Montenegro, Mario Rubens, Mikami, Yuzuru, Miyaji, Makoto, and Nishimura, Kazuko
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- 1998
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22. Climate and acute/subacute paracoccidioidomycosis in a hyper-endemic area in Brazil
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Barrozo, Ligia V, Mendes, Rinaldo P, Marques, Silvio A, Benard, Gil, Silva, Maria E Siqueira, and Bagagli, Eduardo
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- 2009
23. Paracoccidioides brasiliensis antigen batches from the same isolate show immunological and biochemical differences
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Franco, Marcello, Bagagli, Eduardo, Cunha, Marino, Chamma, Luiz Gastão, and Fecchio, Denise
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- 1996
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24. Cryptic Speciation and Recombination in the Fungus Paracoccidioides brasiliensis as Revealed by Gene Genealogies
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Matute, Daniel R., McEwen, Juan G., Puccia, Rosana, Montes, Beatriz A., San-Blas, Gioconda, Bagagli, Eduardo, Rauscher, Jason T., Restrepo, Angela, Morais, Favia, Niño-Vega, Gustavo, and Taylor, John W.
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- 2006
25. Candida tropicalis Systemic Infection Redirects Leukocyte Infiltration to the Kidneys Attenuating Encephalomyelitis.
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Munhoz-Alves, Natália, Nishiyama Mimura, Luiza Ayumi, Viero, Rosa Marlene, Bagagli, Eduardo, Schatzmann Peron, Jean Pierre, Sartori, Alexandrina, and de Campos Fraga-Silva, Thais Fernanda
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CANDIDA tropicalis ,LEUCOCYTES ,ENCEPHALOMYELITIS ,MULTIPLE sclerosis ,PATHOGENESIS ,IMMUNOCOMPROMISED patients - Abstract
Environmental factors, including infections, are strongly associated with the pathogenesis of multiple sclerosis (MS), which is an autoimmune and demyelinating disease of the central nervous system (CNS). Although classically associated with bacterial and viral agents, fungal species have also been suspected to affect the course of the disease. Candida tropicalis is an opportunistic fungus that affects immunocompromised individuals and is also able to spread to vital organs. As C. tropicalis has been increasingly isolated from systemic infections, we aimed to evaluate the effect of this fungus on experimental autoimmune encephalomyelitis (EAE), a murine model to study MS. For this, EAE was induced in female C57BL/6 mice 3 days after infection with 106 viable C. tropicalis yeasts. The infection decreased EAE prevalence and severity, confirmed by the less inflammatory infiltrate and less demyelization in the lumbar spinal cord. Despite this, C. tropicalis infection associated with EAE results in the death of some animals and increased urea and creatinine serum levels. The kidneys of EAE-infected mice showed higher fungal load associated with increased leukocyte infiltration (CD45+ cells) and higher expression of T-box transcription factor (Tbx21) and forkhead box P3 (Foxp3). Altogether, our results demonstrate that although C. tropicalis infection reduces the prevalence and severity of EAE, partially due to the sequestration of leukocytes by the inflamed renal tissue, this effect is associated with a poor disease outcome. [ABSTRACT FROM AUTHOR]
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- 2021
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26. Soil Mycobiome Is Shaped by Vegetation and Microhabitats: A Regional-Scale Study in Southeastern Brazil.
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Hamae Yamauchi, Danielle, Garcia Garces, Hans, de Melo Teixeira, Marcus, Barros Rodrigues, Gabriel Fellipe, Sabrina Ullmann, Leila, Garcia Garces, Adalberto, Hebeler-Barbosa, Flavia, and Bagagli, Eduardo
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ECOLOGICAL niche ,SOIL fungi ,FUNGAL communities ,SPECIES distribution ,GROUND vegetation cover ,ONYGENALES - Abstract
Soil is the principal habitat and reservoir of fungi that act on ecological processes vital for life on Earth. Understanding soil fungal community structures and the patterns of species distribution is crucial, considering climatic change and the increasing anthropic impacts affecting nature. We evaluated the soil fungal diversity in southeastern Brazil, in a transitional region that harbors patches of distinct biomes and ecoregions. The samples originated from eight habitats, namely: semideciduous forest, Brazilian savanna, pasture, coffee and sugarcane plantation, abandoned buildings, owls' and armadillos' burrows. Forty-four soil samples collected in two periods were evaluated by metagenomic approaches, focusing on the high-throughput DNA sequencing of the ITS2 rDNA region in the Illumina platform. Normalized difference vegetation index (NDVI) was used for vegetation cover analysis. NDVI values showed a linear relationship with both diversity and richness, reinforcing the importance of a healthy vegetation for the establishment of a diverse and complex fungal community. The owls' burrows presented a peculiar fungal composition, including high rates of Onygenales, commonly associated with keratinous animal wastes, and Trichosporonales, a group of basidiomycetous yeasts. Levels of organic matter and copper influenced all guild communities analyzed, supporting them as important drivers in shaping the fungal communities' structures. [ABSTRACT FROM AUTHOR]
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- 2021
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27. Zoonotic parasites infecting free‐living armadillos from Brazil.
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Kluyber, Danilo, Desbiez, Arnaud L. J., Attias, Nina, Massocato, Gabriel F., Gennari, Solange M., Soares, Herbert S., Bagagli, Eduardo, Bosco, Sandra M. G., Garcés, Hans G., Ferreira, Jessica da S., Fontes, Amanda N.B, Suffys, Philip N., Meireles, Luciana R., Jansen, Ana M., Luna, Expedito J.A., and Roque, André L. R.
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TRYPANOSOMA cruzi ,HANSEN'S disease ,ARMADILLOS ,MYCOBACTERIUM leprae ,PARASITES ,PARACOCCIDIOIDES brasiliensis - Abstract
Armadillos are specialist diggers and their burrows are used to find food, seek shelter and protect their pups. These burrows can also be shared with dozens of vertebrate and invertebrate species and; consequently, their parasites including the zoonotics. The aim of this study was to diagnose the presence of zoonotic parasites in four wild‐caught armadillo species from two different Brazilian ecosystems, the Cerrado (Brazilian savanna) and the Pantanal (wetland). The investigated parasites and their correspondent diseases were: Toxoplasma gondii (toxoplasmosis), Trypanosoma cruzi (Chagas disease), Leishmania spp., (leishmaniasis), Paracoccidioides brasiliensis (Paracoccidioidomicosis) and Mycobacterium leprae (Hansen's disease). Forty‐three free‐living armadillos from Pantanal and seven road‐killed armadillos from the Cerrado were sampled. Trypanosoma cruzi DTU TcIII were isolated from 2 out of 43 (4.65%) armadillos, including one of them also infected with Trypanosoma rangeli. Antibodies anti‐T. gondii were detected in 13 out of 43 (30.2%) armadillos. All seven armadillos from Cerrado tested positive for P. brasiliensis DNA, in the lungs, spleen, liver fragments. Also, by molecular analysis, all 43 individuals were negative for M. leprae and Leishmania spp. Armadillos were infected by T. cruzi, T. rangeli, P. brasiliensis and presented seric antibodies to T. gondii, highlighting the importance of those armadillos could have in the epidemiology of zoonotic parasites. [ABSTRACT FROM AUTHOR]
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- 2021
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28. Species distribution and susceptibility profile of Candida species in a Brazilian public tertiary hospital
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Montelli Augusto, Sadatsune Terue, Sugizaki Maria, Camargo Carlos, Bruder-Nascimento Ariane, Mondelli Alessandro, and Bagagli Eduardo
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Medicine ,Biology (General) ,QH301-705.5 ,Science (General) ,Q1-390 - Abstract
Abstract Background Species identification and antifungal susceptibility tests were carried out on 212 Candida isolates obtained from bloodstream infections, urinary tract infections and dialysis-associated peritonitis, from cases attended at a Brazilian public tertiary hospital from January 1998 to January 2005. Findings Candida albicans represented 33% of the isolates, Candida parapsilosis 31.1%, Candida tropicalis 17.9%,Candida glabrata 11.8%, and others species 6.2%. In blood culture, C. parapsilosis was the most frequently encountered species (48%). The resistance levels to the antifungal azoles were relatively low for the several species, except for C. tropicalis and C. glabrata. Amphotericin B resistance was observed in 1 isolate of C. parapsilosis. Conclusions The species distribution and antifungal susceptibility herein observed presented several epidemiological features common to other tertiary hospitals in Latin American countries. It also exhibited some peculiarity, such as a very high frequency of C. parapsilosis both in bloodstream infections and dialysis-associated peritonitis. C. albicans also occurred in an important number of case infections, in all evaluated clinical sources. C. glabrata presented a high proportion of resistant isolates. The data emphasize the necessity to carry out the correct species identification accompanied by the susceptibility tests in all tertiary hospitals.
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- 2010
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29. Pathogenicities and GP43kDa gene of three Paracoccidioides brasiliensis isolates originated from a nine-banded armadillo (Dasypus novemcinctus)
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Sano, Ayako, Defaveri, Julio, Tanaka, Reiko, Yokoyama, Koji, Kurita, Nobuyuki, Franco, Marcello, Coelho, Kunie Iabuki R., Bagagli, Eduardo, Montenegro, Mario R., Miyaji, Makoto, and Nishimura, Kazuko
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- 1998
30. Importance of xenarthrans in the eco-epidemiology of Paracoccidioides brasiliensis
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Pedrini Silvia CB, Rosa Patrícia S, Barrozo Lígia, Theodoro Raquel C, Bosco Sandra MG, Richini-Pereira Virgínia B, and Bagagli Eduardo
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Medicine ,Biology (General) ,QH301-705.5 ,Science (General) ,Q1-390 - Abstract
Abstract Background Several pathogens that cause important zoonotic diseases have been frequently associated with armadillos and other xenarthrans. This mammal group typically has evolved on the South American continent and many of its extant species are seriously threatened with extinction. Natural infection of armadillos with Paracoccidioides brasiliensis in hyperendemic areas has provided a valuable opportunity for understanding the role of this mammal in the eco-epidemiology of Paracoccidioidomycosis (PCM), one of the most important systemic mycoses in Latin America. Findings This study aimed to detect P. brasiliensis in different xenarthran species (Dasypus novemcinctus, Cabassous spp., Euphractus sexcinctus, Tamandua tetradactyla and Myrmecophaga tridactyla), by molecular and mycological approaches, in samples obtained by one of the following strategies: i) from road-killed animals (n = 6); ii) from naturally dead animals (n = 8); iii) from animals that died in captivity (n = 9); and iv) from living animals captured from the wild (n = 2). Specific P. brasiliensis DNA was detected in several organs among 7/20 nine-banded armadillos (D. novemcinctus) and in 2/2 anteaters (M. tridactyla). The fungus was also cultured in tissue samples from one of two armadillos captured from the wild. Conclusion Members of the Xenarthra Order, especially armadillos, have some characteristics, including a weak cellular immune response and low body temperature, which make them suitable models for studying host-pathogen interaction. P. brasiliensis infection in wild animals, from PCM endemic areas, may be more common than initially postulated and reinforces the use of these animals as sentinels for the pathogen in the environment.
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- 2009
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31. Paracoccidioides brasiliensis Isolated from Nine-Banded Armadillos (Dasypus novemcinctus) Reveal Population Structure and Admixture in the Amazon Basin.
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Bagagli, Eduardo, Matute, Daniel Ricardo, Garces, Hans Garcia, Tenório, Bernardo Guerra, Garcia Garces, Adalberto, Gomes de Brito Alves, Lucas, Hamae Yamauchi, Danielle, Francisca Hrycyk, Marluce, Barker, Bridget Marie, and de Melo Teixeira, Marcus
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PARACOCCIDIOIDES brasiliensis , *NINE-banded armadillo , *GENOTYPES , *PATHOGENIC fungi , *ARMADILLOS - Abstract
Paracoccidioidomycosis is an endemic fungal disease to Latin America caused by at least five species-level genotypes of Paracoccidioides, named P. lutzii, P. brasiliensis (S1a and S1b populations), P. americana, P. restrepiensis, and P. venezuelensis. In this manuscript, we report on Paracoccidioides sp. sampling efforts in armadillos from two different areas in Brazil. We sequenced the genomes of seven Paracoccidioides isolates and used phylogenomics and populations genetics for genotyping. We found that P. brasiliensis and P. lutzii are both present in the Amazon region. Additionally, we identified two Paracoccidioides isolates that seem to be the result of admixture between divergent populations within P. brasiliensis sensu stricto. Both of these isolates were recovered from armadillos in a P. lutzii endemic area in Midwestern Brazil. Additionally, two isolates from human patients also show evidence of resulting from admixture. Our results suggest that the populations of P. brasiliensis sensu stricto exchange genes in nature. More generally, they suggest that population structure and admixture within species is an important source of variation for pathogenic fungi. [ABSTRACT FROM AUTHOR]
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- 2021
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32. Ecological study of Paracoccidioides brasiliensis in soil: growth ability, conidia production and molecular detection
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Richini-Pereira Virgínia, Macoris Severino, Bosco Sandra, Theodoro Raquel, Reis Gabriela, Bagagli Eduardo, and Terçarioli Gisela
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Microbiology ,QR1-502 - Abstract
Abstract Background Paracoccidioides brasiliensis ecology is not completely understood, although several pieces of evidence point to the soil as its most probable habitat. The present study aimed to investigate the fungal growth, conidia production and molecular pathogen detection in different soil conditions. Methods Soils samples of clayey, sandy and medium textures were collected from ground surface and the interior of armadillo burrows in a hyperendemic area of Paracoccidioidomycosis. P. brasiliensis was inoculated in soil with controlled humidity and in culture medium containing soil extracts. The molecular detection was carried out by Nested PCR, using panfungal and species specific primers from the ITS-5.8S rDNA region. Results The soil texture does not affect fungus development and the growth is more abundant on/in soil saturated with water. Some soil samples inhibited the development of P. brasiliensis, especially those that contain high values of Exchangeable Aluminum (H+Al) in their composition. Some isolates produced a large number of conidia, mainly in soil-extract agar medium. The molecular detection was positive only in samples collected from armadillo burrows, both in sandy and clayey soil. Conclusion P. brasiliensis may grow and produce the infectious conidia in sandy and clayey soil, containing high water content, mainly in wild animal burrows, but without high values of H+Al.
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- 2007
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33. Pulmonary paracoccidioidomycosis‐induced pulmonary hypertension.
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Batah, Sabrina Setembre, Alda, Maiara Almeida, Machado‐Rugulo, Juliana Rodrigues, Felix, Renato Gonçalves, Nascimento, Erika, Martinez, Roberto, Pádua, Adriana Ignácio, Bagagli, Eduardo, Hrycyk, Marluce Francisca, Salgado, Hélio Cesar, Castania, Jaci Airton, Sbragia, Lourenço, Santos, Marcel Koenigkam, Baddini‐Martinez, José Antônio, Morais, Sirlei Siani, Capelozzi, Vera Luiza, Achcar, Rosane Duarte, and Fabro, Alexandre Todorovic
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PULMONARY hypertension ,COCCIDIOIDOMYCOSIS ,HISTOPLASMOSIS ,PARACOCCIDIOIDOMYCOSIS - Abstract
Dear Editor Paraccoccidioidomycosis (PCM) is an endemic disease caused by the fungus I Paracoccidioidomycosis brasilensis i ,1,2 which when inhaled intensely and/or continuously reaches terminal bronchioles and alveoli promoting, in most of the cases, primary pulmonary paracoccidioidomycosis (PPCM).2,3 While effective treatment is available to control PPCM, many patients considered clinically cured have developed pulmonary hypertension (PH). Vascular adventitial layer collagen fibers deposition was significantly increased in PCM patients compared to the chronic obstructive pulmonary disease (COPD)/emphysema group without PH ( I P i < .05)(Figure 2), although COPD/emphysema is a known cause of vascular remodeling. GLO:F2H7/01nov20:ctm2213-fig-0002.jpg PHOTO (COLOR): 2 Human pulmonary paracoccidioidomycosis induced pulmonary hypertension. lung biopsy from PPCM patients and timeframe for development of pulmonary hypertension. [Extracted from the article]
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- 2020
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34. PRP8 intein applied to phylogeny and species identification of dermatophytes
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Bagagli, EDUARDO
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Title: PRP8 intein applied to identification and phylogeny of dermatophytes.Authors: Hans Garcia Garces, Raquel Theodoro Cordeiro, Eduardo Bagagli. Inteins are self-splicing protein elements found in some housekeeping genes important for cell survival. They can be found as a full-length intein when they have a splicing and a homing endonuclease (HE) domains or as a mini-intein when only the splicing domain is present. The PRP8 intein is inserted into the PRP8 protein, a core protein of the spliceosome complex responsible for removing introns during the splicing process. It was observed that variations in the nucleotide sequences of the PRP8 intein reflects the phylogenetic relationships of fungal species and its longitude polymorphism enables a proper molecular identification by electrophoresis techniques. Since a PRP8 intein was by us detected in dermatophytes we aimed to determine the phylogenetic relationship based in PRP8 intein and to identify clinically important Microsporum and Nannizzia species by amplification and visualization of some polymorphic regions (HE region) with no need of sequencing. Forty strains of 11 dermatophytes species were previously molecularly identified (by Internal Transcribed Spacers (ITS) and D1/D2 DNA ribosomal regions) and then, sequenced for PRP8 intein nucleotide region. Nucleotide PRP8 sequences were analyzed and employed for phylogenetic studies by using the software MEGA v 6.0. For electrophoretic molecular identification two sets of primers that amplified a size variable polymorphic region of the HE domain were designed and amplification trough PCR and visualization in agarose gel were performed. The phylogenetic trees obtained by using the PRP8 nucleotide and amino acid sequences were concordant with other phylogenetic constructions that use other genetic regions (ITS and D1/D2). Arthroderma uncinatum was grouped within the Microsporum Clade (M. audouinii and M. canis). E. floccosum clustered within the Nannizzia genus clade, that encompassed the N. incurvata, N. gypsea, N. persicolor and N. fulva species. All Trichophyton species clustered as a separated clade, a proper resolution was achieved within the T. rubrum and T. interdigitale strains. A molecular identification by using amplification by PCR and visualization by agarose gel electrophoresis enabled the identification of herein studied Microsporum and Nannizzia species. Primers HE1F and HE2R were used for identifying Microsporum species (M. audouinii and M. canis), that possessed the biggest amplicon with 938 bp. Trichophyton species (T. rubrum and T. interdigitale) and Nannizia species were separated from Microsporum species with amplicons ranging from 620 to 680 bp. The specific primers HE1F and HE3R made possible to distinguish species within the Nannizzia genus. N. fulva e N. persicolor presented 350 bp, followed by N. gypsea with 327 bp and N. incurvata with 300 bp. We concluded that the nuclear region of the PRP8 intein is a suitable genetic marker for classifying and identifying dermatophytes.
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- 2017
35. Phylogenetic Species of Paracoccidioides spp. Isolated from Clinical and Environmental Samples in a Hyperendemic Area of Paracoccidioidomycosis in Southeastern Brazil.
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Cocio, Tiago A., Nascimento, Erika, von Zeska Kress, Marcia R., Bagagli, Eduardo, and Martinez, Roberto
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PARACOCCIDIOIDES brasiliensis ,PARACOCCIDIOIDOMYCOSIS ,EPIDEMIOLOGY ,HUMAN migrations - Abstract
Paracoccidioides brasiliensis complex and P. lutzii are the etiological agents of paracoccidioidomycosis. The geographic distribution of these species in South America is still poorly comprehended. Fifty samples of Paracoccidioides spp. were genotyped, with 46 clinical isolates predominantly isolated in the geographic area of Ribeirão Preto, SP, and four environmental isolates collected in Ibiá, MG, southeastern Brazil. These isolates were evaluated by PCR-RFLP (Restriction Fragment Length Polymorphism) of the tub1 gene and the sequencing of the gp43 exon 2 loci. The species P. lutzii was confirmed by sequencing the internal transcribed spacer (ITS) region of the ribosomal DNA. P. brasiliensis sensu stricto S1b (n = 42) and S1a (n = 5), P. americana (n = 1), P. restrepiensis (n = 1), and P. lutzii (n = 1) were identified among the clinical isolates. All the environmental isolates were characterized as P. brasiliensis sensu stricto S1b. The patient infection by P. lutzii, P. americana (PS2), and one isolate of P. brasiliensis sensu stricto S1b most likely occurred in a geographic area far from the fungal isolation site. No association was found between the infecting genotype and the disease form. These results expand the knowledge of the Paracoccidioides species distribution and emphasize that human migration must also be considered to pinpoint the genotypes in the endemic area. [ABSTRACT FROM AUTHOR]
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- 2020
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36. Rabies virus and Histoplasma suramericanum coinfection in a bat from southeastern Brazil.
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Menozzi, Benedito Donizete, Paz, Giselle Souza, Paiz, Laís Moraes, Garces, Hans Garcia, Adorno, Brunna Mayla Vasconcelos, Almeida‐Silva, Fernando, Zancope Oliveira, Rosely Maria, Richini‐Pereira, Virgínia Bodelão, Chechi, Jéssica Luana, Bagagli, Eduardo, Bosco, Sandra de Moraes Gimenes, and Langoni, Helio
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RABIES virus ,EMERGING infectious diseases ,MIXED infections ,BATS ,CITY dwellers ,PSYCHIATRIC hospitals ,PUBLIC hospitals - Abstract
Bats are essential to the global ecosystem, but their ability to harbour a range of pathogens has been widely discussed, as well as their role in the emergence and re‐emergence of infectious diseases. This paper describes the first report of coinfection by two zoonotic agents, rabies virus (RABV) and the fungus Histoplasma suramericanum in a bat. The bat was from the Molossus molossus species, and it was found during the daytime in the hallway of a public psychiatric hospital in a municipality in São Paulo State, southeastern Brazil. RABV infection was diagnosed by the direct fluorescent antibody test and mouse inoculation test. The fungus was isolated by in vitro culture. Both diagnoses were confirmed by molecular techniques. Phylogenetic analysis showed that the fungus isolate had proximity to H. suramericanum in the Lam B clade, while the RABV isolate was characterized in the Lasiurus cinereus lineage. Since the M. molossus bat was found in a peri‐urban transition area (urban/peri‐urban), the possibility of cross‐species transmission of this RABV lineage becomes more plausible, considering that this scenario may provide shelter for both M. molossus and L. cinereus. These are relevant findings since there has been an increase in bat populations in urban and peri‐urban areas, particularly due to environmental modifications and anthropogenic impacts on their habitat. Thus, the detection of two zoonotic agents in a bat found in a public hospital should raise awareness regarding the importance of systematic surveillance actions directed towards bats in urban areas. [ABSTRACT FROM AUTHOR]
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- 2020
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37. Paracoccidioidomycosis due to Paracoccidioides lutzii complicated with adrenal injury and pulmonary arterial hypertension.
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Gambero Gaspar, Gilberto, Alexandre Cocio, Tiago, Guioti-Puga, Fernanda, Nascimento, Erika, Todorovic Fabro, Alexandre, von Zeska Kress, Marcia Regina, Bagagli, Eduardo, and Martinez, Roberto
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Paracoccidioidomycosis caused by Paracoccidioides lutzii is endemic in the Midwest of Brazil and its clinical spectrum is still little known due to the recent identification of this fungal species. A patient resident in Southeast Brazil, but who had lived for many years in the Midwest region, presented with skin injuries, chronic cough and bilateral adrenal involvement. Paracoccidioides spp. was isolated in culture from a skin lesion biopsy. This isolate was later identified as P. lutzii using gene sequencing. A favorable initial response to treatment with itraconazole was observed, but a few weeks later, the patient developed respiratory failure and worsening of lung lesions. Evaluation by computed tomography and echocardiography were suggestive of pulmonary arterial hypertension, and a bronchoscopic biopsy showed peribronchial remodeling. The patient completed the antifungal treatment but maintained the respiratory dysfunction. The reported case shows that P. lutzii can be isolated from patients in a geographic area far from the place of infection acquisition and that, as P. brasiliensis, it can cause adrenal injury and cardio-respiratory complications as a consequence of excessive necrosis and fibrosis. [ABSTRACT FROM AUTHOR]
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- 2020
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38. A hidden battle in the dirt: Soil amoebae interactions with Paracoccidioides spp.
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Albuquerque, Patrícia, Nicola, André Moraes, Magnabosco, Diogo Almeida Gomes, Derengowski, Lorena da Silveira, Crisóstomo, Luana Soares, Xavier, Luciano Costa Gomes, Frazão, Stefânia de Oliveira, Guilhelmelli, Fernanda, de Oliveira, Marco Antônio, Dias, Jhones do Nascimento, Hurtado, Fabián Andrés, Teixeira, Marcus de Melo, Guimarães, Allan Jefferson, Paes, Hugo Costa, Bagagli, Eduardo, Felipe, Maria Sueli Soares, Casadevall, Arturo, and Silva-Pereira, Ildinete
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SOIL fungi ,CILIATA ,AMOEBA ,PATTERN perception receptors ,MOLECULAR recognition ,ACANTHAMOEBA castellanii ,FUNGAL virulence - Abstract
Paracoccidioides spp. are thermodimorphic fungi that cause a neglected tropical disease (paracoccidioidomycosis) that is endemic to Latin America. These fungi inhabit the soil, where they live as saprophytes with no need for a mammalian host to complete their life cycle. Despite this, they developed sophisticated virulence attributes allowing them not only to survive in host tissues but also to cause disease. A hypothesis for selective pressures driving the emergence or maintenance of virulence of soil fungi is their interaction with soil predators such as amoebae and helminths. We evaluated the presence of environmental amoeboid predators in soil from armadillo burrows where Paracoccidioides had been previously detected and tested if the interaction of Paracoccidioides with amoebae selects for fungi with increased virulence. Nematodes, ciliates, and amoebae–all potential predators of fungi–grew in cultures from soil samples. Microscopical observation and ITS sequencing identified the amoebae as Acanthamoeba spp, Allovahlkampfia spelaea, and Vermamoeba vermiformis. These three amoebae efficiently ingested, killed and digested Paracoccidioides spp. yeast cells, as did laboratory adapted axenic Acanthamoeba castellanii. Sequential co-cultivation of Paracoccidioides with A. castellanii selected for phenotypical traits related to the survival of the fungus within a natural predator as well as in murine macrophages and in vivo (Galleria mellonella and mice). These changes in virulence were linked to the accumulation of cell wall alpha-glucans, polysaccharides that mask recognition of fungal molecular patterns by host pattern recognition receptors. Altogether, our results indicate that Paracoccidioides inhabits a complex environment with multiple amoeboid predators that can exert selective pressure to guide the evolution of virulence traits. [ABSTRACT FROM AUTHOR]
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- 2019
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39. Paracoccidioides brasiliensis AND Paracoccidioides lutzii, A SECRET LOVE AFFAIR
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ARANTES, Thales Domingos, BAGAGLI, Eduardo, NIÑO-VEGA, Gustavo, SAN-BLAS, Gioconda, THEODORO, Raquel Cordeiro, Universidade Estadual Paulista (Unesp), Laboratório de Virologia e Micologia, Centro de Microbiología y Biología Celular, and Depto. De Biologia Celular e Genética
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Ecology ,Paracoccidioides lutzii ,Molecular phylogeny ,Paracoccidioides brasiliensis ,Biology ,Dimorphism - Abstract
Made available in DSpace on 2018-12-11T16:58:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-09-01. Added 1 bitstream(s) on 2019-10-09T18:30:00Z : No. of bitstreams: 1 S0036-46652015000800025.pdf: 126533 bytes, checksum: 9236dc3d6017b1691b85e36acfe43ab8 (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) To commemorate Prof. Carlos da Silva Lacaz’s centennial anniversary, the authors have written a brief account of a few, out of hundreds, biological, ecological, molecular and phylogenetic studies that led to the arrival of Paracoccidioides lutzii, hidden for more than a century within Paracoccidioides brasiliensis. Lacaz’s permanent interest in this fungus, and particularly his conviction on the benefits that research on paracoccidioidomycosis would bring to patients, were pivotal in the development of the field. Universidade Estadual Paulista Instituto de Biociências/UNESP Depto. Microbiologia e Imunologia Instituto de Medicina Tropical/IMT Laboratório de Virologia e Micologia Campus Universitário Lagoa Nova Instituto Venezolano de Investigaciones Científicas (IVIC) Laboratorio de Micología Centro de Microbiología y Biología Celular Universidade Federal do Rio Grande do Norte Depto. De Biologia Celular e Genética Campus Universitário Lagoa Nova Universidade Estadual Paulista Instituto de Biociências/UNESP Depto. Microbiologia e Imunologia FAPESP: 2012/03233-3 FAPESP: 2012/14047-6
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- 2015
40. Ecology of Paracoccidioides brasiliensis, P. lutzii and related species: infection in armadillos, soil occurrence and mycological aspects.
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Hrycyk, Marluce Francisca, Garces, Hans Garcia, Bosco, Sandra de Moraes Gimenes, Oliveira, Sílvio Luis de, Marques, Sílvio Alencar, and Bagagli, Eduardo
- Abstract
Paracoccidioides brasiliensis and the related species P. americana, P. restrepiensis, P. venezuelensis, and P. lutzii (Ascomycota, Ajellomycetaceae) are the etiological agents of paracoccidoidoimycosis (PCM), one of the most important systemic mycoses in Latin America. They are dimorphic fungi, with a mycelial life cycle in soil and a yeast phase associated with tissues of mammalian hosts. This study aimed to detect Paracoccidioides spp. in armadillo tissues and associated soil samples in three well-defined geographic areas, including the Alta Floresta, an area not only endemic for PCM in the central region of Brazil but also of probable P. lutzii occurrence, whose ecology and geographic distribution are poorly elucidated. The isolates were genotyped by sequencing ITS-rDNA and the gp43 -exon-2 region, and by PCR-RFLP of alpha tubulin (tub1) gene; mycological aspects such as yeast-to-mycelial transition, growth and conidial production in soil extract agar were also evaluated. We confirmed that while armadillos are highly infected by P. brasiliensis, including multiple infections by distinct genotypes or species (P. brasiliensis and P. americana) in the same animal, the same does not hold true for P. lutzii, which in turn seems to present less capacity for mycelial growth and conidial production, when developing in a soil-related condition. [ABSTRACT FROM AUTHOR]
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- 2018
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41. Molecular identification and phylogenetic analysis of Bothrops insularis bacterial and fungal microbiota.
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Barbosa, Lidiane Nunes, Ferreira Jr, Rui Seabra, Luiza Mello, Priscila, Garcia Garces, Hans, Luana Chechi, Jéssica, Frachin, Tarsila, De Barros, Luciana Curtolo, De Moraes Guimenes Bosco, Sandra, Bagagli, Eduardo, Fernandes Júnior, Ary, Barraviera, Benedito, and Delazari Dos Santos, Lucilene
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BOTHROPS ,FUNGI ,BACTERIA - Abstract
Bothrops insularis, known as the golden lancehead snake, has its natural habitat restricted to Queimada Grande Island on the southern coast of Brazil. This culture-dependent study aimed to identify microorganisms obtained from the mouth, eyes, and cloaca of this species. Swabs from 20 snakes were collected for fungal and bacterial isolation. DNA was extracted from all samples, and identification was performed by amplifying the ITS1-5.8S-ITS2 regions and the 16S rDNA gene, respectively. All strains were identified and deposited in the GenBank nucleotide database. MEGA v6.0 software was utilized to construct phylogenetic trees. In total, 100 strains were isolated and characterized, from which 42 fungi were distributed into 23 species and 58 bacteria into 13 species. The genusFusariumwas predominant since 11 strains and probably a new species was isolated from this fungus.Pseudomonas aeruginosaandEnterococcus faecaliswere the predominant groups of aerobic bacteria isolated. Phylogenetic analyses between bacterial and fungal sequences suggest a similarity between the microorganisms found on the island and on the continent. These findings may be attributed to anthropic actions resulting from both expeditions to the island and actions of migratory birds, which are the main sources of food for snakes. [ABSTRACT FROM PUBLISHER]
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- 2018
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42. Molecular identification and phylogenetical analysis of dermatophyte fungi from Latin America.
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Garcia Garces, Hans, Hrycyk, Marluce F., Giacobino, Juliana, Capela Machado, Gabriel, Domingos Arantes, Thales, Theodoro, Raquel C., Bosco, Sandra de M. G., and Bagagli, Eduardo
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FUNGAL phylogeny ,DERMATOPHYTES ,IDENTIFICATION of fungi ,MOLECULAR biology - Abstract
Dermatophytes constitute a complex group of fungi, comprised of by the genera Trichophyton, Epidermophyton and Microsporum. They have the ability to degrade keratin and cause human and animal infections. Molecular techniques have made their identification faster and more accurate, and allowed important advances in phylogenetic studies. We aim to identify molecularly and to determine the phylogenetic relationships in dermatophyte fungi from Brazil and other Latin American countries, using DNA sequencing of the nuclear ribosome regions ITS1-5.8S- ITS2 and D1/D2. DNA of 45 dermatophytes was extracted and amplified by PCR for identification at the species level by sequencing of those ribosomal regions. The software mega 6.0 was used to establish the phylogenetic relationships via the Maximum Likelihood method. Out of 45 strains, 43 were identified by ITS (95.5%) and 100% by D1/D2 sequencing. Two strains could not be identified by ITS. Phylogenetic analyses separated the genera Trichophyton and Microsporum, which presented an uncertain relationship with Epidermophyton floccosum, depending on the ribosomal marker. Both regions can provide efficient identification of dermatophytes, whereas phylogenetic analysis revealed complex relations among dermatophyte fungi. [ABSTRACT FROM AUTHOR]
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- 2016
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43. Multiple Origins of the Pathogenic Yeast Candida orthopsilosis by Separate Hybridizations between Two Parental Species.
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Schröder, Markus S., Martinez de San Vicente, Kontxi, Prandini, Tâmara H. R., Hammel, Stephen, Higgins, Desmond G., Bagagli, Eduardo, Wolfe, Kenneth H., and Butler, Geraldine
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CANDIDA ,FUNGAL hybridization ,ISOLATION of biotechnological microorganisms ,FUNGAL genomes ,PLANT-fungus relationships ,SINGLE nucleotide polymorphisms ,PLANT evolution - Abstract
Mating between different species produces hybrids that are usually asexual and stuck as diploids, but can also lead to the formation of new species. Here, we report the genome sequences of 27 isolates of the pathogenic yeast Candida orthopsilosis. We find that most isolates are diploid hybrids, products of mating between two unknown parental species (A and B) that are 5% divergent in sequence. Isolates vary greatly in the extent of homogenization between A and B, making their genomes a mosaic of highly heterozygous regions interspersed with homozygous regions. Separate phylogenetic analyses of SNPs in the A- and B-derived portions of the genome produces almost identical trees of the isolates with four major clades. However, the presence of two mutually exclusive genotype combinations at the mating type locus, and recombinant mitochondrial genomes diagnostic of inter-clade mating, shows that the species C. orthopsilosis does not have a single evolutionary origin but was created at least four times by separate interspecies hybridizations between parents A and B. Older hybrids have lost more heterozygosity. We also identify two isolates with homozygous genomes derived exclusively from parent A, which are pure non-hybrid strains. The parallel emergence of the same hybrid species from multiple independent hybridization events is common in plant evolution, but is much less documented in pathogenic fungi. [ABSTRACT FROM AUTHOR]
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- 2016
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44. Evolution and Application of Inteins in Candida species: A Review.
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Fernandes, José A. L., Prandini, Tâmara H. R., da Conceição A. Castro, Maria, Arantes, Thales D., Giacobino, Juliana, Bagagli, Eduardo, and Theodoro, Raquel C.
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RNA splicing ,GENETIC regulation ,CANDIDA - Abstract
Inteins are invasive intervening sequences that perform an autocatalytic splicing from their host proteins. Among eukaryotes, these elements are present in many fungal species, including those considered opportunistic or primary pathogens, such as Candida spp. Here we reviewed and updated the list of Candida species containing inteins in the genes VMA, THRRS and GLT1 and pointed out the importance of these elements as molecular markers for molecular epidemiological researches and speciesspecific diagnosis, since the presence, as well as the size of these inteins, is polymorphic among the different species. Although absent in Candida albicans, these elements are present in different sizes, in some environmental Candida spp. and also in most of the non-albicans Candida spp. considered emergent opportunistic pathogens. Besides, the possible role of these inteins in yeast physiology was also discussed in the light of the recent findings on the importance of these elements as post-translational modulators of gene expression, reinforcing their relevance as alternative therapeutic targets for the treatment of non-albicans Candida infections, because, once the splicing of an intein is inhibited, its host protein, which is usually a housekeeping protein, becomes non-functional. [ABSTRACT FROM AUTHOR]
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- 2016
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45. Fungal peritonitis in patients undergoing peritoneal dialysis (PD) in Brazil: molecular identification, biofilm production and antifungal susceptibility of the agents.
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Giacobino, Juliana, Montelli, Augusto Cezar, Barretti, Pasqual, Bruder-Nascimento, Ariane, Caramori, Jacqueline Teixeira, Barbosa, Luciano, and Bagagli, Eduardo
- Abstract
This paper presents data on fungal peritonitis (FP) in patients undergoing peritoneal dialysis (PD) at the University Hospital of Botucatu Medical School, São Paulo, Brazil. In a total of 422 patients, 30 developed FP, from which the medical records and the fungal isolates of 23 patient cases were studied. All patients presented abdominal pain, cloudy peritoneal effluent, needed hospitalization, had the catheter removed and were treated with fluconazole or fluconazole plus 5-flucitosine; six of them died due to FP. Concerning the agents, it was observed that Candida parapsilosis was the leading species (9/23), followed by Candida albicans (5/23), Candida orthopsilosis (4/23), Candida tropicalis (3/23), Candida guilliermondii (1/23), and Kodamaea ohmeri (1/23). All the isolates were susceptible to amphotericin B, voriconazole and caspofungin whereas C. albicans isolates were susceptible to all antifungals tested. Resistance to fluconazole was observed in three isolates of C. orthopsilosis, and dose-dependent susceptibility to this antifungal was observed in two isolates of C. parapsilosis and in the K. ohmeri isolate. Biofilm production estimates were high or moderate in most isolates, especially in C. albicans species, and low in C. parapsilosis species, with a marked variation among the isolates. This Brazilian study reinforces that FP in PD is caused by a diverse group of yeasts, most prevalently C. parapsilosis sensu stricto species. In addition, they present significant variation in susceptibility to antifungals and biofilm production, thus contributing to the complexity and severity of the clinical features. [ABSTRACT FROM AUTHOR]
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- 2016
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46. Genome Diversity, Recombination, and Virulence across the Major Lineages of Paracoccidioides.
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Muñoz, José F., Farrer, Rhys A., Desjardins, Christopher A., Gallo, Juan E., Sykes, Sean, Sakthikumar, Sharadha, Misas, Elizabeth, Whiston, Emily A., Bagagli, Eduardo, Soares, Celia M. A., de M. Teixeira, Marcus, Taylor, John W., Clay, Oliver K., McEwen, Juan G., and Cuomo, Christina A.
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- 2016
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47. Comparison of methods for the identification of microorganisms isolated from blood cultures.
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Marinho Monteiro, Aydir Cecília, Branco Fortaleza, Carlos Magno Castelo, Ferreira, Adriano Martison, de Souza Cavalcante, Ricardo, Mondelli, Alessandro Lia, Bagagli, Eduardo, and de Souza da Cunha, Maria de Lourdes Ribeiro
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MICROBIOLOGY ,MICROORGANISMS ,BLOOD diseases ,BODY fluids ,HUMAN body - Abstract
Background: Bloodstream infections are responsible for thousands of deaths each year. The rapid identification of the microorganisms causing these infections permits correct therapeutic management that will improve the prognosis of the patient. In an attempt to reduce the time spent on this step, microorganism identification devices have been developed, including the VITEK® 2 system, which is currently used in routine clinical microbiology laboratories. Methods: This study evaluated the accuracy of the VITEK® 2 system in the identification of 400 microorganisms isolated from blood cultures and compared the results to those obtained with conventional phenotypic and genotypic methods. In parallel to the phenotypic identification methods, the DNA of these microorganisms was extracted directly from the blood culture bottles for genotypic identification by the polymerase chain reaction (PCR) and DNA sequencing. Results: The automated VITEK® 2 system correctly identified 94.7 % (379/400) of the isolates. The YST and GN cards resulted in 100 % correct identifications of yeasts (15/15) and Gram-negative bacilli (165/165), respectively. The GP card correctly identified 92.6 % (199/215) of Gram-positive cocci, while the ANC card was unable to correctly identify any Gram-positive bacilli (0/5). Conclusions: The performance of the VITEK® 2 system was considered acceptable and statistical analysis showed that the system is a suitable option for routine clinical microbiology laboratories to identify different microorganisms. [ABSTRACT FROM AUTHOR]
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- 2016
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48. Correction: Environmental Mapping of Paracoccidioides spp. in Brazil Reveals New Clues into Genetic Diversity, Biogeography and Wild Host Association.
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Arantes, Thales Domingos, Theodoro, Raquel Cordeiro, Teixeira, Marcus de Melo, Bosco, Sandra de Moraes Gimenes, and Bagagli, Eduardo
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ENVIRONMENTAL mapping ,PARACOCCIDIOIDES brasiliensis ,BIOGEOGRAPHY - Abstract
A correction to the article "Environmental Mapping of Paracoccidioides spp. in Brazil Reveals New Clues into Genetic Diversity, Biogeography and Wild Host Association" that was published in the April 29, 2016 issue is presented.
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- 2016
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49. Environmental Mapping of Paracoccidioides spp. in Brazil Reveals New Clues into Genetic Diversity, Biogeography and Wild Host Association.
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Arantes, Thales Domingos, Theodoro, Raquel Cordeiro, Teixeira, Marcus de Melo, Bosco, Sandra de Moraes Gimenes, and Bagagli, Eduardo
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ENVIRONMENTAL mapping ,PARACOCCIDIOIDES brasiliensis ,BACTERIAL genetics ,ETIOLOGY of diseases ,PARACOCCIDIOIDOMYCOSIS ,MOLECULAR biology ,BIOGEOGRAPHY - Abstract
Background: Paracoccidioides brasiliensis and Paracoccidioides lutzii are the etiological agents of Paracoccidioidomycosis (PCM), and are easily isolated from human patients. However, due to human migration and a long latency period, clinical isolates do not reflect the spatial distribution of these pathogens. Molecular detection of P. brasiliensis and P. lutzii from soil, as well as their isolation from wild animals such as armadillos, are important for monitoring their environmental and geographical distribution. This study aimed to detect and, for the first time, evaluate the genetic diversity of P. brasiliensis and P. lutzii for Paracoccidioidomycosis in endemic and non-endemic areas of the environment, by using Nested PCR and in situ hybridization techniques. Methods/Principal Findings: Aerosol (n = 16) and soil (n = 34) samples from armadillo burrows, as well as armadillos (n = 7) were collected in endemic and non-endemic areas of PCM in the Southeastern, Midwestern and Northern regions of Brazil. Both P. brasiliensis and P. lutzii were detected in soil (67.5%) and aerosols (81%) by PCR of Internal Transcribed Spacer (ITS) region (60%), and also by in situ hybridization (83%). Fungal isolation from armadillo tissues was not possible. Sequences from both species of P. brasiliensis and P. lutzii were detected in all regions. In addition, we identified genetic Paracoccidioides variants in soil and aerosol samples which have never been reported before in clinical or armadillo samples, suggesting greater genetic variability in the environment than in vertebrate hosts. Conclusions/Significance: Data may reflect the actual occurrence of Paracoccidioides species in their saprobic habitat, despite their absence/non-detection in seven armadillos evaluated in regions with high prevalence of PCM infection by P. lutzii. These results may indicate a possible ecological difference between P. brasiliensis and P. lutzii concerning their wild hosts. [ABSTRACT FROM AUTHOR]
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- 2016
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50. Paracoccidioides Species Complex: Ecology, Phylogeny, Sexual Reproduction, and Virulence.
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Teixeira, Marcus M., Theodoro, Raquel C., Nino-Vega, Gustavo, Bagagli, Eduardo, and Felipe, Maria S. S.
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PARACOCCIDIOIDOMYCOSIS ,PUBLIC health ,PHYLOGENY ,PARACOCCIDIOIDES brasiliensis ,MICROBIAL virulence ,DIMORPHISM (Biology) - Abstract
The article offers information on the incidence of paracoccidioidomycosis (PCM) in Latin America. Topics discussed include the phylogeny of paracoccidioides genus, ecology of paracoccidioides brasiliensis and paracoccidioides lutzii, sexual reproduction of the paracoccidioides species complex, virulence factors associated with dimorphism and host adaptation, and impact of paracoccidioides species complex on clinical and serological aspects of PCM.
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- 2014
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