43 results on '"Baptista, Tiago"'
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2. Lessons in looking : the digital audiovisual essay
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Baptista, Tiago
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791.43 - Abstract
This thesis examines the contemporary practice of the digital audiovisual essay, which is defined as a material form of thinking at the crossroads of academic textual analysis, personal cinephilia, and popular online fandom practices, to suggest that it allows rich epistemological discoveries not only about individual films and viewing experiences, but also about how cinema is perceived in the context of digitally mediated audiovisual culture. Chapter one advances five key defining tensions of the digital audiovisual essay: its object is the investigation of specific films and cinephiliac experiences; it uses a performative research methodology based on the affordances of digital viewing and editing technologies; it exists primarily in Web 2.0 and takes advantage of its collaborative and dialogical modes of production; it is a “rich text object” that continuously tests the different contributions of both verbal and audiovisual forms of communication to the production of knowledge about the cinema; and finally, the digital audiovisual essay has an important pedagogical potential, not only for those who watch it, but especially for those who practice it. Chapter two presents the theoretical framework of the dissertation, challenges the ‘newness’ of the digital audiovisual essay, and suggests that any investigation of this cultural practice must address its ideological implications and its role in the context of contemporary audiovisual culture. Accordingly, it relates the editing and compositional techniques of the digital audiovisual essay with modernist montage and suggests that the audiovisual essay has not only inherited, but has also updated and enhanced the dialectical interdependency between critical and consumerism drives that shaped modernism’s ambiguous relation to mass culture. The final chapter examines four case studies (David Bordwell, Catherine Grant, ::kogonada, and Kevin B. Lee) that showcase the contradictory tensions of this cultural practice and broad our understanding of the politics of the digital audiovisual essay.
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- 2016
3. SAGA Is a General Cofactor for RNA Polymerase II Transcription
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Baptista, Tiago, Grünberg, Sebastian, Minoungou, Nadège, Koster, Maria J.E., Timmers, H.T. Marc, Hahn, Steve, Devys, Didier, and Tora, László
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- 2017
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4. Transcription of Nearly All Yeast RNA Polymerase II-Transcribed Genes Is Dependent on Transcription Factor TFIID
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Warfield, Linda, Ramachandran, Srinivas, Baptista, Tiago, Devys, Didier, Tora, Laszlo, and Hahn, Steven
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- 2017
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5. The European Portuguese Version of the Brief Negative Symptom Scale.
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Santos, Henrique Castro, Rodrigues, Alexandra, Ferreira, Sara, Martins, João Malhadas, Baptista, Tiago, Gama Marques, João, Kirkpatrick, Brian, and Prata, Diana
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PSYCHOSES ,SYMPTOMS ,TEST validity ,SCHIZOAFFECTIVE disorders - Abstract
Negative symptoms reflect a currently much-untreated loss of normal functioning and are frequently found in psychotic disorders. We present the first translation of the Brief Negative Symptom Scale (BNSS) to European Portuguese and evaluate its validity in a sample of Portuguese male patients with a psychotic spectrum disorder. The Portuguese BNSS showed excellent internal consistency, high convergent validity (i.e., strong correlation with the PANSS negative factor), and high discriminant validity (i.e., a lack of association with the PANSS positive factor). In sum, the present European Portuguese BNSS has shown to be reliable, thus extending this instrument's clinical availability worldwide. [ABSTRACT FROM AUTHOR]
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- 2024
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6. Fast scan, an improved approach using machine learning for vulnerability identification
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Baptista, Tiago João Fernandes, Henriques, Pedro Rangel, Oliveira, Nuno, and Universidade do Minho
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Vulnerabilidade ,Vulnerability ,Attention models ,Security ,Segurança ,Modelos de atenção ,Atatic analysis ,Análise estática ,Engenharia e Tecnologia::Engenharia Eletrotécnica, Eletrónica e Informática - Abstract
Dissertação de mestrado integrado em Engenharia Informática, This document presents a Master Thesis in the Integrated Master’s in Informatics Engi neering focused on the automatic identification of vulnerabilities, that was accomplished at Universidade do Minho in Braga, Portugal. This thesis work aims at developing a machine learning based tool for automatic iden tification of vulnerabilities on programs (source, high level code), that uses an abstract syntax11tree representation. It is based on FastScan, using code2seq approach. Fastscan is a recently developed system aimed capable of detecting vulnerabilities in source code using machine learning techniques. Nevertheless, FastScan is not able of identifying the vulnerability type. In the presented work the main goal is to go further and develop a method to identify specific types of vulnerabilities. As will be shown, the goal will be achieved by changing the method of receiving and processing in a different way the input data and developing an architecture that brings together multiple models to predict different specific vulnerabilities. The best f1 metric obtained is 93% resulting in a precision of 90% and accuracy of 85%, according to the performed tests and regarding a trained model to predict vulnerabilities of the injection type. These results were obtained with the contribution given by the optimization of the model’s hyperparameters and also the use of the Search Cluster from University of Minho that greatly diminished the necessary time to perform training and testing. It is important to refer that overfitting was detected in the late stages of the tests, so this results do not represent the true value in real context. Also an interface is presented, it allows to better interact with the models and analyse the scan results., Este documento apresenta uma dissertação do Mestrado Integrado em Engenharia Infor mática, que tem como foco a automação da deteção de vulnerabilidades e foi concluída na Universidade do Minho em Braga, Portugal. O trabalho apresentado nesta tese pretende desenvolver uma ferramenta que utiliza machine learning e que seja capaz de identificar vulnerabilidades em código. Utilizando para isso a representação do mesmo numa abstract syntax tree. Tem como base FastScan que utiliza a abordagem do code2seq. Fastscan é um projeto recentemente desenvolvido que é capaz de detetar vulnerabilidades em código utilizando técnicas de machine learning, sendo que tem algumas lacunas como o facto de não ser capaz de identificar vulnerabilidades específicas. No trabalho apresentado o objetivo é ir mais além e desenvolver um método capaz de identificar qual o tipo específico de vulnerabilidade presente. Como será apresentado ao longo do documento, este objetivo será alcançado pela alteração do método de receção e processamento dos dados recebidos, assim como o desenvolvimento de uma arquitetura que junte os vários modelos de maneira a cooperarem e a ferramenta ser capaz de detetar e prever a presença de vulnerabilidades específicas. A melhor métrica de f1 obtida foi de 93%, com precisão de 90% e accuracy de 85%, de acordo com os testes efetuados sobre um modelo treinado para prever a presença de vulnerabilidades do tipo de injection. Os resultados foram obtidos devido à otimização dos hiper-parâmetros dos modelos e o cluster Search da Universidade do Minho diminuiu consideravelmente o tempo necessário para efetuar o traino e testes dos modelos. É importante referir que foi detetado overfitting na fase final do desenvolvimento deste trabalho, sendo que os resultados apresentados não representam o valor real dos modelos em contexto real. Para além disso é apresentada uma interface que permite interagir e analisar os resultados de um scan feito pelos modelos.
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- 2022
7. Neuroimaging findings of babies with microcephaly and presumed congenital Zika virus infection
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Baptista, Tiago, Quaghebeur, Gerardine, and Alarcon, Ana
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- 2016
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8. Phenotypic Impact of Deregulated Expression of Class I Histone Deacetylases in Urothelial Cell Carcinoma of the Bladder
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Junqueira-Neto, Susana, Vieira, Filipa Q., Montezuma, Diana, Costa, Natália R., Antunes, Luís, Baptista, Tiago, Oliveira, Ana Isabel, Graça, Inês, Rodrigues, Ângelo, Magalhães, José S., Oliveira, Jorge, Henrique, Rui, and Jerónimo, Carmen
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- 2015
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9. Coccygeoplasty: preliminary experience with this new alternative treatment of refractory coccydynia in patients with coccyx hypermobility.
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Manfre, Luigi, Gil, Inês, Baptista, Tiago, Pires, Pedro Calvão, de Vivo, Aldo Eros, Masala, Salvador, Chandra, Ronil V., D'Anna, Gennaro, and Hirsch, Joshua A.
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EHLERS-Danlos syndrome ,CHRONIC pain ,PAIN ,PAIN measurement ,PELVIC pain ,COCCYX ,LOCAL anesthesia ,RETROSPECTIVE studies ,BACKACHE ,MAGNETIC resonance imaging ,BACK ,TREATMENT effectiveness ,COMPARATIVE studies ,DESCRIPTIVE statistics ,ALTERNATIVE medicine ,COMPUTED tomography ,COCCYDYNIA ,LONGITUDINAL method - Abstract
Background Coccydynia has many causes, including fracture, subluxation, and hypermobility of sacrococcygeal segments. Existing treatments are limited in their effectiveness. Coccygeoplasty (CP) is a relatively new, minimally invasive treatment that appears to address this difficult clinical challenge. Objective To describe clinical results at the time of the procedure and at 3- and 12-months' follow-up of patients with coccydynia related to subluxation and coccyx hypermobility treated with the CP technique. Additionally, to determine if there is any correlation between the final imaging and clinical results at 3- and 12-months' follow-up. Methods A prospectively maintained database was used, and all patients who underwent CP for chronic coccydynia between January 2005 and October 2018 were retrospectively reviewed. All the patients had painful hypermobility (greater than 25°) with anterior flexion confirmed on radiological imaging. Alternative causes of coccydynia were excluded using CT and MRI. Procedures were performed under local anesthesia with combined fluoroscopic and CT guidance. Clinical followup was performed at two time points: 3 and 12 months after treatment using the Visual Analogue Scale (VAS). Results Twelve patients were treated in a single center. No procedural complications occurred. At 3- and 12-months' follow-up, the majority (75%) of patients had significantly lower VAS scores than at baseline, with mean changes of 3.5 and 4.9, respectively. There was no pain recurrence at 12 months and just one patient had no improvement of the pain. Follow- up CT images confirmed fixation of the sacrococcygeal bone segments in nine patients; however, no correlation was found between final imaging results and clinical outcome (p=0.1). Conclusions Patients with refractory painful coccyx subluxation and hypermobility undergoing CP have a favorable clinical response at 3- and 12-months' followup. Further studies are required to validate this technique and to identify predictors of treatment response. Coccygeoplasty may be considered a reasonable alternative to coccygectomy. [ABSTRACT FROM AUTHOR]
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- 2023
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10. Epigenetic regulation of EFEMP1 in prostate cancer: biological relevance and clinical potential
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Almeida, Mafalda, Costa, Vera L., Costa, Natália R., Ramalho-Carvalho, João, Baptista, Tiago, Ribeiro, Franclim R., Paulo, Paula, Teixeira, Manuel R., Oliveira, Jorge, Lothe, Ragnhild A., Lind, Guro E., Henrique, Rui, and Jerónimo, Carmen
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- 2014
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11. Using Machine Learning for Vulnerability Detection and Classification
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Baptista, Tiago, Oliveira, Nuno, and Henriques, Pedro Rangel
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Machine Learning ,Source Code Analysis ,Security and privacy → Vulnerability scanners ,Computing methodologies → Machine learning ,Vulnerability Detection - Abstract
The work described in this paper aims at developing a machine learning based tool for automatic identification of vulnerabilities on programs (source, high level code), that uses an abstract syntax tree representation. It is based on FastScan, using code2seq approach. Fastscan is a recently developed system aimed capable of detecting vulnerabilities in source code using machine learning techniques. Nevertheless, FastScan is not able of identifying the vulnerability type. In the presented work the main goal is to go further and develop a method to identify specific types of vulnerabilities. As will be shown, the goal will be achieved by optimizing the model’s hyperparameters, changing the method of preprocessing the input data and developing an architecture that brings together multiple models to predict different specific vulnerabilities. The preliminary results obtained from the training stage, are very promising. The best f1 metric obtained is 93% resulting in a precision of 90% and accuracy of 85%, according to the performed tests and regarding a trained model to predict vulnerabilities of the injection type., OASIcs, Vol. 94, 10th Symposium on Languages, Applications and Technologies (SLATE 2021), pages 14:1-14:14
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- 2021
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12. Evolution of a multi-agent system in a cyclical environment
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Baptista, Tiago and Costa, Ernesto
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- 2008
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13. Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
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Baptista, Tiago, Devys, Didier, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), and Tora, Laszlo
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[SDV] Life Sciences [q-bio] ,[SDV]Life Sciences [q-bio] ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
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- 2018
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14. Étude fonctionelle du coactivateur SAGA : rôle global dans la transcription par l’ARN Polymérase II
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Fidalgo Baptista, Tiago and STAR, ABES
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genetic processes ,information science ,health occupations ,TFIID ,ARN Polymérase II ,S. cerevisiae ,[SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,SAGA ,macromolecular substances ,RNA Polymerase II ,Transcription - Abstract
Prior studies suggested that SAGA and TFIID are alternative factors that promote RNA polymerase II (RNA Pol II) transcription with about 10% of genes in S. cerevisiae dependent on SAGA. The remainder 90% of the genome would be regulated by TFIID. We reassessed the role of SAGA by mapping its genome-wide location and role in global transcription in budding yeast. We observed that SAGA maps to regulatory elements of most genes, irrespective of previous designations of SAGA- or TFIID-dominated genes. Additionally, disruption of either SAGA or TFIID through mutation or rapid subunit depletion reduces transcription from nearly all genes, measured by newly-synthesized RNA or RNA Pol II chromatin immunoprecipitation. We also found that the acetyltransferase Gcn5 synergizes with Spt3 to promote global transcription and that Spt3 functions to stimulate TBP recruitment at all tested genes. Our data demonstrate that both SAGA and TFIID act as general cofactors required for essentially all RNA Pol II transcription and is not consistent with the previous classification of SAGA- and TFIID-dominated genes., Des études antérieures suggèrent que les complexes SAGA et TFIID sont des facteurs jouant un rôle complémentaire dans la transcription par l’ARN polymérase II. Chez S. cerevisiae, environ 10% des gènes semblent dépendants de SAGA alors que TFIID aurait un rôle dominant sur 90% du génome. De nouvelles approches m’ont permis de montrer que SAGA est recruté sur les régions régulatrices d’une majorité de gènes, indépendamment de leur classification. Des analyses d’ARN nouvellement-synthétisés ont également démontré que l’inactivation des complexes SAGA ou TFIID, par mutation ou déplétion inductible de leurs sous-unités, altèrent la transcription de pratiquement tous les gènes par l’ARN polymérase II. L’acétyltransférase Gcn5 et la sous-unité Spt3 agissent de façon synergique au sein du complexe SAGA pour stimuler le recrutement de TBP et la transcription par l’ARN polymérase II. Ces données indiquent que les complexes SAGA et TFIID agissent comme des cofacteurs généraux, étant nécessaires pour la synthèse de quasiment tous les ARNm et ayant des effets équivalents sur les gènes précédemment décrits comme dominés par SAGA ou par TFIID.
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- 2017
15. Phenotypic impact of deregulated expression of class I histone deacetylases in urothelial cell carcinoma of the bladder
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Neto, Susana, Quintela Vieira, Ana Filipa, Montezuma, Diana, Costa, Natália R., Antunes, Luís, Baptista, Tiago, Oliveira, Ana Isabel, Pinho dos Santos Graça, Maria Inês, Rodrigues, Ângelo, Magalhães, José S., Oliveira, Jorge, Henrique, Rui, Jerónimo, Carmen, and Repositório Científico do Instituto Politécnico do Porto
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Bladder Neoplasms ,Urinary Bladder ,RNA ,Aged - Abstract
Submitted by Biblioteca Escola Superior de Saúde (biblioteca@ess.ipp.pt) on 2019-01-30T17:52:29Z No. of bitstreams: 1 ART_VieiraAna_2013.pdf: 1521656 bytes, checksum: 968082c63d858c3513ceec645a27968e (MD5) Approved for entry into archive by Biblioteca Escola Superior de Saúde (biblioteca@ess.ipp.pt) on 2019-01-30T17:55:14Z (GMT) No. of bitstreams: 1 ART_VieiraAna_2013.pdf: 1521656 bytes, checksum: 968082c63d858c3513ceec645a27968e (MD5) Approved for entry into archive by Biblioteca Escola Superior de Saúde (biblioteca@ess.ipp.pt) on 2019-01-30T18:06:23Z (GMT) No. of bitstreams: 1 ART_VieiraAna_2013.pdf: 1521656 bytes, checksum: 968082c63d858c3513ceec645a27968e (MD5) Made available in DSpace on 2019-01-30T18:06:23Z (GMT). No. of bitstreams: 1 ART_VieiraAna_2013.pdf: 1521656 bytes, checksum: 968082c63d858c3513ceec645a27968e (MD5) Previous issue date: 2015 info:eu-repo/semantics/publishedVersion
- Published
- 2015
16. Computational Fluid Dynamics in patients treated with flow-diverter devices
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Baptista, Tiago
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genetic structures ,Computer Applications-3D ,education ,Interventional vascular ,Computer applications ,CNS ,Aneurysms ,Image manipulation / Reconstruction ,Catheter arteriography - Abstract
Learning objectives Background Findings and procedure details Conclusion Personal information References, Learning objectives: The objective of this study is to simulate, by using computational fluid dynamics, the haemodynamic conditions in pre-treatment aneurysms and correlate that features with the follow-up observations after treatment with flow-diverter...
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- 2015
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17. Delaying cinema: an interview with Laura Mulvey
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Baptista, Tiago Ribeiro Ramos and Veritati - Repositório Institucional da Universidade Católica Portuguesa
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Submitted by Agostinho Macau (arm@ucp.pt) on 2021-09-15T14:47:14Z No. of bitstreams: 1 58_Texto_Artigo_171_2_10_20140211.pdf: 1035426 bytes, checksum: f45fe97e604f86ccc491a9fe6d38e522 (MD5) Made available in DSpace on 2021-09-15T14:47:14Z (GMT). No. of bitstreams: 1 58_Texto_Artigo_171_2_10_20140211.pdf: 1035426 bytes, checksum: f45fe97e604f86ccc491a9fe6d38e522 (MD5) Previous issue date: 2014-01-28 info:eu-repo/semantics/publishedVersion
- Published
- 2014
18. Film/Cinema (Portugal)
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Baptista, Tiago
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World War, 1914-1918--Motion pictures and the war ,Weltkrieg [1914-1918] ,Film - Abstract
This article discusses the production and reception of Portuguese and foreign “war movies” in Portugal during 1914-1918. It focuses on non-fiction cinema but includes a brief description of the only Portuguese fiction film about the war, ''João Ratão'' [Jorge Brum do Canto, 1940]. Special attention is given to the Portuguese Army’s official film service and its inability to make any films about the Portuguese troops fighting on the European front. The article ends by considering the role of war movies in Portuguese film culture and their role in the process of institutionalization of cinema in this country during the 1910s., 1914-1918-Online International Encyclopedia of the First World War
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- 2014
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19. SAGA Is a General Cofactor for RNA Polymerase II Transcription
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Baptista, Tiago, Grünberg, Sebastian, Minoungou, Nadège, Koster, Maria J.E., Timmers, H.T. Marc, Hahn, Steve, Devys, Didier, and Tora, László
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- 2018
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20. Morphology and Ontogeny of Statoliths in the Grooved Carpet Shell, Ruditapes decussatus.
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Galante-Oliveira, Susana, Pereira, António, Baptista, Tiago, Guimarães, Fernanda, Soares, Jorge, Lopes, José Carlos, Machado, Jorge, and Barroso, Carlos
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- 2019
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21. Complexity and emergence in societies of agents
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Baptista, Tiago Rodrigues and Costa, Ernesto Jorge Fernandes
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Sistemas complexos ,Computação evolucionária ,Vida artificial ,Sistemas multi-agente - Abstract
Tese de doutoramento em Ciências (Engenharia Informática), apresentada à Faculdade de Ciências e Tecnologia da Universidade de Coimbra Throughout the last decades, Darwin’s theory of natural selection has fuelled a vast amount of research in the field of computer science, and more specifically in artificial intelligence. The majority of this work has focussed on artificial selection, rather than on natural selection. In parallel, a growing interest in complexity science brought new modelling paradigms into the scene, with a focus on bottom-up approaches. By combining ideas from complex systems research and artificial life, we present a multi-agent simulation model for open-ended evolution, and a software framework (BitBang) that implements it. We also present a rule list based algorithm implemented for the brain component of the agents. Genetic variation operators were created to drive the evolution of the rule list brains. Several simulation environments were created using the BitBang framework. Experimental results are presented and analysed, validating our model. The results presented show that the model is capable of evolving agents’ controllers in an open-ended evolution simulation. We see that populations evolve sustainable reproduction behaviours, without hard-coding the reproduction conditions into the simulations. By providing evolutionary pressure through the modelling of the environment, we see that on increasingly complex environments, agents evolve increasingly complex behaviours. The rule list brain is shown to provide an important analysis advantage by having readability into the agents’ evolved behaviours. This feature proved to be especially important when unexpected behaviours emerged. Financial support by Fundação para a Ciência e a Tecnologia (FCT) through PhD grant SFRH/BD/18401/2004
- Published
- 2012
22. Focal brain stem tumors in childhood
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Baptista, Tiago
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Oncology ,Neoplasia ,Neuroradiology brain ,Neuro - Tumor ,Neuroradiology peripheral nerve ,Neuroradiology spine - Abstract
Learning objectives Background Imaging findings OR Procedure details Conclusion Personal Information References, Learning objectives: To demonstrate the different locations of focal brainstem tumors in children. To explain the utility of MRI and MR-spectroscopy in the diagnosis and follow-up of these tumors. To confirm that in children, focal brainstem tumors have a better...
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- 2010
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23. Which Buyer-Supplier Strategies on Uncertain Markets? A Multi-Agents Simulation
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Curchod, Corentin, Caillou, Philippe, Baptista, Tiago, Audencia Recherche, Audencia Business School, Laboratoire de Recherche en Informatique (LRI), Université Paris-Sud - Paris 11 (UP11)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), Machine Learning and Optimisation (TAO), Université Paris-Sud - Paris 11 (UP11)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-Inria Saclay - Ile de France, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), Departamento de Engenharia Informática / Department of Informatics Engineering (DEI), University of Coimbra [Portugal] (UC)-Universidade de Coimbra [Coimbra], Centre National de la Recherche Scientifique (CNRS)-Inria Saclay - Ile de France, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Université Paris-Sud - Paris 11 (UP11)-Laboratoire de Recherche en Informatique (LRI), and Université Paris-Sud - Paris 11 (UP11)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-CentraleSupélec
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[INFO.INFO-MA]Computer Science [cs]/Multiagent Systems [cs.MA] - Abstract
International audience; The relationship the firm has with its clients and suppliers largely determines the amount of value it is able to capture in the chain. Yet little is known about the impact of market uncertainty on the choice of the best buyer-supplier strategy in terms of value appropriation. The paper proposes to investigate which among different types of strategies bring more value to the buyer when market uncertainty increases. It relies on a very innovative method of multi-agents simulation based on an in-depth ethnographic observation phase of a real market in France. It shows that pure loyalty is not necessarily the best strategy when market uncertainty increases and that the buyer may face a dilemma between maximizing the margin rate and ensuring regularity of supply.
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- 2009
24. Multi-agent Based simulation for Decision-Making: an application to Rungis food market
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Caillou, Philippe, Baptista, Tiago, Curchod, Corentin, Laboratoire de Recherche en Informatique (LRI), Université Paris-Sud - Paris 11 (UP11)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), Machine Learning and Optimisation (TAO), Centre National de la Recherche Scientifique (CNRS)-Inria Saclay - Ile de France, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Université Paris-Sud - Paris 11 (UP11)-Laboratoire de Recherche en Informatique (LRI), Université Paris-Sud - Paris 11 (UP11)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-CentraleSupélec, Departamento de Engenharia Informática / Department of Informatics Engineering (DEI), University of Coimbra [Portugal] (UC)-Universidade de Coimbra [Coimbra], Audencia Recherche, Audencia Business School, Joao Climaco, Gregory Kersten, Joao Paulo Costa, Université Paris-Sud - Paris 11 (UP11)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-Inria Saclay - Ile de France, and Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)
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[INFO.INFO-MA]Computer Science [cs]/Multiagent Systems [cs.MA] - Abstract
International audience; In this paper, we present our first results obtained with a multi-agent based simulation of the fruit and vegetable Rungis market. Our goal is to simulate negotiation and price formation based on observed strategies on the real market. We aim to understand negotiation and price formation mechanisms, and explain their impact on macro variables (like the difference between the official price and the average price used on the market).
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- 2008
25. The most Portuguese village in Portugal: tradition in the age of its technical reproducibility
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Baptista, Tiago
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Cultural objectification ,Ethnographic film ,Portuguese Cinema - Abstract
Ethnographic film is often associated with many European countries’ past as colonial powers and the way these countries used film to depict African, American and Asian territories and populations they once ruled. However, ethnographic film also has a European tradition of its own, closely interlaced with the history of ethnography and anthropology as autonomous sciences and with the desire of scholars to represent local, regional and national cultural identities. This paper presents a Portuguese attempt of this sort dating from 1938, when the authoritarian regime organized a national contest to determine which would be Portugal’s most “authentic” village – something other European countries also did. As part of this metonymic contribution to the construction of Portugal’s national identity as an agrarian utopia, a short documentary was shot, sponsored by the same official propaganda office that had organized the contest. In this film, the viewer’s gaze is made to coincide with the one of the national jury visiting the final selection of 12 villages and to whose benefit local scholars had organized all sorts of colourful peasant traditions hoping to cause the strongest impression. The film makes a strong case for the importance of ethnographic film as a relevant instance not only of the iteration of existing European national cultures, but also of the construction of so many of Europe’s national identities and traditions. Suffice to say that even today the village of “Monsanto”, which won the 1938 contest, is still referred to as “Portugal’s most Portuguese village”.
- Published
- 2006
26. Architecture of TAF11/TAF13/TBP complex suggests novel regulation properties of general transcription factor TFIID.
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Gupta, Kapil, Watson, Aleksandra A., Baptista, Tiago, Scheer, Elisabeth, Chambers, Anna L., Koehler, Christine, Zou, Juan, Obong-Ebong, Ima, Kandiah, Eaazhisai, Temblador, Arturo, Round, Adam, Forest, Eric, Man, Petr, Bieniossek, Christoph, Laue, Ernest D., Lemke, Edward A., Rappsilber, Juri, Robinson, Carol V., Devys, Didier, and Tora, Làszlò
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- 2017
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27. Automatic Step Evolution.
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Baptista, Tiago and Costa, Ernesto
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- 2014
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28. Step evolution: Improving the performance of open-ended evolution simulations.
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Baptista, Tiago and Costa, Ernesto
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- 2013
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29. Secondary Trigeminal Neuralgia: Do Not Forget the Pontine Ischemic Infarction.
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Cabral, Gonçalo, Lorga, Tiago, Caetano, André, Baptista, Tiago, and Baptista, Miguel Viana
- Subjects
TRIGEMINAL neuralgia ,INFARCTION ,CEREBRAL infarction ,FACIAL pain ,TRIGEMINAL nerve ,DEMYELINATION - Abstract
Keywords: Secondary; Trigeminal neuralgia; Pontine; Infarction EN Secondary Trigeminal neuralgia Pontine Infarction 391 392 2 08/20/21 20210901 NES 210901 Secondary trigeminal neuralgia (TN) is mainly associated with neoplasms and demyelinating diseases. TN due to pontine infarction is a rare entity and often overlooked with only few cases reported, but should be considered in the differential diagnosis of secondary TN [[2]]. [Extracted from the article]
- Published
- 2021
- Full Text
- View/download PDF
30. Simulation of the Rungis Wholesale Market: Lessons on the Calibration, Validation and Usage of a Cognitive Agent-Based Simulation.
- Author
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Caillou, Philippe, Curchod, Corentin, and Baptista, Tiago
- Published
- 2009
- Full Text
- View/download PDF
31. Enoxacin inhibits growth of prostate cancer cells and effectively restores microRNA processing.
- Author
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Sousa, Elsa J., Graça, Inês, Baptista, Tiago, Vieira, Filipa Q., Palmeira, Carlos, Henrique, Rui, and Jerónimo, Carmen
- Published
- 2013
- Full Text
- View/download PDF
32. Epigenetic regulation of MDR1 gene through posttranslational histone modifications in prostate cancer.
- Author
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Henrique, Rui, Oliveira, Ana Isabel, Costa, Vera L., Baptista, Tiago, Martins, Ana Teresa, Morais, António, Oliveira, Jorge, and Jerónimo, Carmen
- Subjects
PROSTATE cancer & genetics ,MULTIDRUG resistance ,HISTONE genetics ,DNA methyltransferases ,TRICHOSTATIN A ,EPIGENETICS - Abstract
Background Multidrug resistance 1 (MDR1) gene encodes for an ATP binding cassette transporter - Pglycoprotein (P-gp) - involved in chemoresistance to taxanes. MDR1 promoter methylation is frequent in prostate carcinoma (PCa), suggesting an epigenetic regulation but no functional correlation has been established. We aimed to elucidate the epigenetic mechanisms involved in MDR1 deregulation in PCa. Results MDR1 promoter methylation and P-gp expression were assessed in 121 PCa, 39 high-grade prostatic intraepithelial neoplasia (HGPIN), 28 benign prostatic hyperplasia (BPH) and 10 morphologically normal prostate tissue (NPT) samples, using quantitative methylation specific PCR and immunohistochemistry, respectively. PCa cell lines were exposed to a DNA methyltransferases inhibitor 5-aza-2'deoxycytidine (DAC) and histone deacetylases inhibitor trichostatin A (TSA). Methylation and histone posttranscriptional modifications status were characterized and correlated with mRNA and protein expression. MDR1 promoter methylation levels and frequency significantly increased from NPTs, to HGPIN and to PCa. Conversely, decreased or absent P-gp immunoexpression was observed in HGPIN and PCa, inversely correlating with methylation levels. Exposure to DAC alone did not alter significantly methylation levels, although increased expression was apparent. However, P-gp mRNA and protein re-expression were higher in cell lines exposed to TSA alone or combined with DAC. Accordingly, histone active marks H3Ac, H3K4me2, H3K4me3, H3K9Ac, and H4Ac were increased at the MDR1 promoter after exposure to TSA alone or combined with DAC. Conclusion Our data suggests that, in prostate carcinogenesis, MDR1 downregulation is mainly due to histone post-translational modifications. This occurs concomitantly with aberrant promoter methylation, substantiating the association with P-gp decreased expression. CpG island hypermethylation, epigenetic regulation, histone post-translational activation/repression marks, MDR1, P-gp, prostate. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
33. "il faut voir le maî": A Recent Restoration of Surgical FIlms by E.-L. Doyen (1859-1916).
- Author
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Baptista, Tiago
- Abstract
The article discusses Cinematica Portuguesa-Museu do Cinema's identification and restoration of the surgical films of surgeon Eugène-Louis Doyen. Apart from a few copies held by French film archives, most of his film collection was believed to be lost. Doyen used films as a way to legitimate his new surgical techniques among the international medical community. The material history of Doyen's film collection, from the shooting of the films to their disappearance, as well as the history of all the different exhibition and distribution strategies Doyen employed, has been reconstructed in several articles.
- Published
- 2005
34. Lessons in looking : the digital audiovisual essay
- Author
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Baptista, Tiago
- Subjects
hafvm - Abstract
This thesis examines the contemporary practice of the digital audiovisual essay, which is defined as a material form of thinking at the crossroads of academic textual analysis, personal cinephilia, and popular online fandom practices, to suggest that it allows rich epistemological discoveries not only about individual films and viewing experiences, but also about how cinema is perceived in the context of digitally mediated audiovisual culture. Chapter one advances five key defining tensions of the digital audiovisual essay: its object is the investigation of specific films and cinephiliac experiences; it uses a performative research methodology based on the affordances of digital viewing and editing technologies; it exists primarily in Web 2.0 and takes advantage of its collaborative and dialogical modes of production; it is a “rich text object” that continuously tests the different contributions of both verbal and audiovisual forms of communication to the production of knowledge about the cinema; and finally, the digital audiovisual essay has an important pedagogical potential, not only for those who watch it, but especially for those who practice it. Chapter two presents the theoretical framework of the dissertation, challenges the ‘newness’ of the digital audiovisual essay, and suggests that any investigation of this cultural practice must address its ideological implications and its role in the context of contemporary audiovisual culture. Accordingly, it relates the editing and compositional techniques of the digital audiovisual essay with modernist montage and suggests that the audiovisual essay has not only inherited, but has also updated and enhanced the dialectical interdependency between critical and consumerism drives that shaped modernism’s ambiguous relation to mass culture. The final chapter examines four case studies (David Bordwell, Catherine Grant, ::kogonada, and Kevin B. Lee) that showcase the contradictory tensions of this cultural practice and broad our understanding of the politics of the digital audiovisual essay.
35. Enoxacin inhibits growth of prostate cancer cells and restores microRNA processing.
- Author
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Sousa, Elsa, Graça, Inês, Baptista, Tiago, Vieira, Filipa Q., Palmeira, Carlos, Henrique, Rui, and Jerónimo, Carmen
- Published
- 2013
- Full Text
- View/download PDF
36. The SAGA coactivator complex acts on the whole transcribed genome and is required for RNA polymerase II transcription.
- Author
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Bonnet, Jacques, Chen-Yi Wang, Baptista, Tiago, Vincent, Stéphane D., Wei-Chun Hsiao, Stierle, Matthieu, Cheng-Fu Kao, Tora, Laszló, and Devys, Didier
- Subjects
- *
ACETYLTRANSFERASES , *CHROMATIN-remodeling complexes , *GENE expression , *UBIQUITINATION , *MESSENGER RNA , *RNA polymerases - Abstract
The SAGA (Spt-Ada-Gcn5 acetyltransferase) coactivator complex contains distinct chromatin-modifying activities and is recruited by DNA-bound activators to regulate the expression of a subset of genes. Surprisingly, recent studies revealed little overlap between genome-wide SAGA-binding profiles and changes in gene expression upon depletion of subunits of the complex. As indicators of SAGA recruitment on chromatin, we monitored in yeast and human cells the genome-wide distribution of histone H3K9 acetylation and H2B ubiquitination, which are respectively deposited or removed by SAGA. Changes in these modifications after inactivation of the corresponding enzyme revealed that SAGA acetylates the promoters and deubiquitinates the transcribed region of all expressed genes. In agreement with this broad distribution, we show that SAGA plays a critical role for RNA polymerase II recruitment at all expressed genes. In addition, through quantification of newly synthesized RNA, we demonstrated that SAGA inactivation induced a strong decrease of mRNA synthesis at all tested genes. Analysis of the SAGA deubiquitination activity further revealed that SAGA acts on the whole transcribed genome in a very fast manner, indicating a highly dynamic association of the complex with chromatin. Thus, our study uncovers a new function for SAGA as a bone fide cofactor for all RNA polymerase II transcription. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
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37. Neuroimaging findings of babies with microcephaly and presumed congenital Zika virus infection
- Author
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Baptista, Tiago, Quaghebeur, Gerardine, and Alarcon, Ana
38. Strategies for the preservation of a b/w motion picture film collection in safety film support
- Author
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Somfai, Teréz, Ramos, Ana, Baptista, Tiago, and Roldão, Élia
- Subjects
condition assessment ,motion picture storage ,magnetic sound ,safety film ,preservation strategies ,Engenharia e Tecnologia::Outras Engenharias e Tecnologias [Domínio/Área Científica] - Abstract
This thesis aims to contribute for reviewing Safety film preservation guidelines currently in practice, concerning film identification, film assessment and storage conditions in the archive, Arquivo Nacional das Imagens em Movimento (ANIM – National Archive of Moving Images), conservation center of Cinemateca Portuguesa. Since the 1980s, stability issues and degradation factors concerning the oldest type of safety film, cellulose triacetate (CTA), have been studied. Based on the results accomplished with those studies, preservation guidelines were established highlighting the advantages of low temperature and low relative humidity for film stability. Despite the guideline’s disclosure, there are practical issues, such as lack of staff, financial restraints and lack of studies regarding polyethylene terephthalate (PET) films decay, that ultimately jeopardize the preservation of motion film collections. In order to have an overall comprehension of the identification and assessment methods used, a review of relevant literature about national and international guidelines for film preservation was performed. This review endowed a broader understanding of the identification, assessment and monitorization carried out in ANIM archive. Aiming to understand preservation practices, an investigation was undertaken at ANIM. Current identification and assessment methods were reexamined, tested and reevaluated. Based on the results accomplished, new identification and assessment tools, as well as, recommendations concerning organization and storage are proposed. For the experimental part of this work, identification and assessment of film (image and sound) were performed. For this internship and study, it was decided firstly to perform the identification and assessment of 100 films and film cans according to the macro identification and macro assessment methods described in the literature. For macro assessment, Acid Detection (A-D) strips and macro signs of degradation were used to evaluate the condition of the films studied. Moreover, the macro assessment results obtained in 2006 and 2014 were also analyzed. In order to identify possible degradation trends and contribute to the review of the current preservation practice, past and present macro assessment results were compared. Additionally, new assessment methods, such as hardness measurement, hardness (Shore A), were tested. The results obtained confirmed that free acidity tests are effective methods for monitoring safety film decay. In this study it was also possible to identify degradation trends, specifically concerning magnetic tapes. Moreover, besides CTA film decay, during the macro assessment of ANIM’s collection, PET films degraded were found. Based on these results it was possible to propose a new methodology for film preservation.
- Published
- 2019
39. Nationwide Access to Endovascular Treatment for Acute Ischemic Stroke in Portugal.
- Author
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Dias MC, Dos Reis RS, Santos JV, Nunes AP, Ferreira P, Maia B, Fragata I, Reis J, Lopes JR, Cruz L, Santo G, Machado E, Gabriel D, Felgueiras R, Dória HM, Carneiro A, Correia M, Veloso LM, Barros P, Gregorio T, Carvalho A, Ribeiro M, Teotonio P, Neto L, E Melo TP, Canhao P, Filipe JP, Moreira G, Azevedo E, Silva ML, Costa EC, Oliveira G, Pereira L, Neves L, Rodrigues M, Marto JP, Calado S, Grenho F, Branco G, Baptista T, Rocha J, Ferreira C, Pinho J, Amorim JM, Araujo JM, Neiva RM, Viana J, Lobo M, Freitas A, Cruz VT, Sargento-Freitas J, and Lopes JC
- Subjects
- Cohort Studies, Humans, Portugal, Retrospective Studies, Treatment Outcome, Brain Ischemia therapy, Endovascular Procedures, Ischemic Stroke, Stroke etiology, Stroke therapy
- Abstract
Introduction: Since the publication of endovascular treatment trials and European Stroke Guidelines, Portugal has re-organized stroke healthcare. The nine centers performing endovascular treatment are not equally distributed within the country, which may lead to differential access to endovascular treatment. Our main aim was to perform a descriptive analysis of the main treatment metrics regarding endovascular treatment in mainland Portugal and its administrative districts., Material and Methods: A retrospective national multicentric cohort study was conducted, including all ischemic stroke patients treated with endovascular treatment in mainland Portugal over two years (July 2015 to June 2017). All endovascular treatment centers contributed to an anonymized database. Demographic, stroke-related and procedure-related variables were collected. Crude endovascular treatment rates were calculated per 100 000 inhabitants for mainland Portugal, and each district and endovascular treatment standardized ratios (indirect age-sex standardization) were also calculated. Patient time metrics were computed as the median time between stroke onset, first-door, and puncture., Results: A total of 1625 endovascular treatment procedures were registered. The endovascular treatment rate was 8.27/100 000 inhabitants/year. We found regional heterogeneity in endovascular treatment rates (1.58 to 16.53/100 000/year), with higher rates in districts closer to endovascular treatment centers. When analyzed by district, the median time from stroke onset to puncture ranged from 212 to 432 minutes, reflecting regional heterogeneity., Discussion: Overall endovascular treatment rates and procedural times in Portugal are comparable to other international registries. We found geographic heterogeneity, with lower endovascular treatment rates and longer onset-to-puncture time in southern and inner regions., Conclusion: The overall national rate of EVT in the first two years after the organization of EVT-capable centers is one of the highest among European countries, however, significant regional disparities were documented. Moreover, stroke-onset-to-first-door times and in-hospital procedural times in the EVT centers were comparable to those reported in the randomized controlled trials performed in high-volume tertiary hospitals.
- Published
- 2022
- Full Text
- View/download PDF
40. Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity.
- Author
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Baptista T and Devys D
- Subjects
- Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins metabolism, Thiouracil chemistry, RNA Polymerase II metabolism, RNA, Messenger genetics, Thiouracil analogs & derivatives
- Abstract
Global defects in RNA polymerase II transcription might be overlooked by transcriptomic studies analyzing steady-state RNA. Indeed, the global decrease in mRNA synthesis has been shown to be compensated by a simultaneous decrease in mRNA degradation to restore normal steady-state levels. Hence, the genome-wide quantification of mRNA synthesis, independently from mRNA decay, is the best direct reflection of RNA polymerase II transcriptional activity. Here, we discuss a method using non-perturbing metabolic labeling of nascent RNAs in Saccharomyces cerevisiae (S. cerevisiae). Specifically, the cells are cultured for 6 min with a uracil analog, 4-thiouracil, and the labeled newly transcribed RNAs are purified and quantified to determine the synthesis rates of all individual mRNA. Moreover, using labeled Schizosaccharomyces pombe cells as internal standard allows comparing mRNA synthesis in different S. cerevisiae strains. Using this protocol and fitting the data with a dynamic kinetic model, the corresponding mRNA decay rates can be determined.
- Published
- 2018
- Full Text
- View/download PDF
41. Expression and functionality of histone H2A variants in cancer.
- Author
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Monteiro FL, Baptista T, Amado F, Vitorino R, Jerónimo C, and Helguero LA
- Subjects
- Animals, Gene Expression, Histones biosynthesis, Histones genetics, Humans, Neoplasms genetics, Proto-Oncogene Mas, Histones metabolism, Neoplasms metabolism
- Abstract
Regulation of gene expression includes the replacement of canonical histones for non-allelic histone variants, as well as their multiple targeting by postranslational modifications. H2A variants are highly conserved between species suggesting they execute important functions that cannot be accomplished by canonical histones. Altered expression of many H2A variants is associated to cancer. MacroH2A variants are enriched in heterocromatic foci and necessary for chromatin condensation. MacroH2A1.1 and macroH2A1.2 are two mutually exclusive isoforms. MacroH2A1.1 and macroH2A2 inhibit proliferation and are associated with better cancer prognosis; while macroH2A1.2 is associated to cancer progression. H2AX variant functions as a sensor of DNA damage and defines the cellular response towards DNA repair or apoptosis; therefore, screening approaches and therapeutic options targeting H2AX have been proposed. H2A.Z is enriched in euchromatin, acting as a proto-oncogene with established roles in hormone responsive cancers and overexpressed in endocrine-resistant disease. Other H2A family members have also been found altered in cancer, but their function remains unknown. Substantial progress has been made to understand histone H2A variants, their contribution to normal cellular function and to cancer development and progression. Yet, implementation of high resolution mass spectrometry is needed to further our knowledge on highly homologous H2A variants expression and function.
- Published
- 2014
- Full Text
- View/download PDF
42. Anti-tumoral effect of the non-nucleoside DNMT inhibitor RG108 in human prostate cancer cells.
- Author
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Graça I, Sousa EJ, Baptista T, Almeida M, Ramalho-Carvalho J, Palmeira C, Henrique R, and Jerónimo C
- Subjects
- Antineoplastic Agents administration & dosage, Antineoplastic Agents pharmacology, Apoptosis drug effects, Cell Line, Tumor, Dose-Response Relationship, Drug, Epigenesis, Genetic, Gene Expression Regulation, Neoplastic drug effects, Gene Silencing, Humans, Male, Phthalimides administration & dosage, Promoter Regions, Genetic, Prostatic Neoplasms pathology, RNA, Messenger metabolism, Reverse Transcriptase Polymerase Chain Reaction, Time Factors, Tryptophan administration & dosage, Tryptophan pharmacology, DNA (Cytosine-5-)-Methyltransferases antagonists & inhibitors, DNA Methylation, Phthalimides pharmacology, Prostatic Neoplasms drug therapy, Tryptophan analogs & derivatives
- Abstract
Background: Current therapeutic strategies for advanced prostate cancer (PCa) are largely ineffective. Because aberrant DNA methylation associated with inappropriate gene-silencing is a common feature of PCa, DNA methylation inhibitors might constitute an alternative therapy. In this study we aimed to evaluate the anti-cancer properties of RG108, a novel non-nucleoside inhibitor of DNA methyltransferases (DNMT), in PCa cell lines., Methods: The anti-tumoral impact of RG108 in LNCaP, 22Rv1, DU145 and PC-3 cell lines was assessed through standard cell viability, apoptosis and cell cycle assays. Likewise, DNMT activity, DNMT1 expression and global levels of DNA methylation were evaluated in the same cell lines. The effectiveness of DNA demethylation was further assessed through the determination of promoter methylation and transcript levels of GSTP1, APC and RAR-β2, by quantitative methylation-specific PCR and RT-PCR, respectively., Results: RG108 led to a significant dose and time dependent growth inhibition and apoptosis induction in LNCaP, 22Rv1 and DU145. LNCaP and 22Rv1 also displayed decreased DNMT activity, DNMT1 expression and global DNA methylation. Interestingly, chronic treatment with RG108 significantly decreased GSTP1, APC and RAR-β2 promoter hypermethylation levels, although mRNA reexpression was only attained for GSTP1 and APC., Conclusions: RG108 is an effective tumor growth suppressor in most PCa cell lines tested. This effect is likely mediated by reversion of aberrant DNA methylation affecting cancer related-genes epigenetically silenced in PCa. However, additional mechanism might underlie the anti-tumor effects of RG108. In vivo studies are now mandatory to confirm these promising results and evaluate the potential of this compound for PCa therapy.
- Published
- 2014
- Full Text
- View/download PDF
43. Regulation of histone H2A.Z expression is mediated by sirtuin 1 in prostate cancer.
- Author
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Baptista T, Graça I, Sousa EJ, Oliveira AI, Costa NR, Costa-Pinheiro P, Amado F, Henrique R, and Jerónimo C
- Subjects
- Aged, Cell Line, Tumor, Gene Expression Regulation, Neoplastic, Histones biosynthesis, Histones metabolism, Humans, Male, Middle Aged, Prostatic Neoplasms genetics, Prostatic Neoplasms metabolism, Prostatic Neoplasms pathology, Protein Processing, Post-Translational, Sirtuin 1 metabolism, Histones genetics, Sirtuin 1 genetics
- Abstract
Histone variants seem to play a major role in gene expression regulation. In prostate cancer, H2A.Z and its acetylated form are implicated in oncogenes' upregulation. SIRT1, which may act either as tumor suppressor or oncogene, reduces H2A.Z levels in cardiomyocytes, via proteasome-mediated degradation, and this mechanism might be impaired in prostate cancer cells due to sirtuin 1 downregulation. Thus, we aimed to characterize the mechanisms underlying H2A.Z and SIRT1 deregulation in prostate carcinogenesis and how they interact. We found that H2AFZ and SIRT1 were up- and downregulated, respectively, at transcript level in primary prostate cancer and high-grade prostatic intraepithelial neoplasia compared to normal prostatic tissues. Induced SIRT1 overexpression in prostate cancer cell lines resulted in almost complete absence of H2A.Z. Inhibition of mTOR had a modest effect on H2A.Z levels, but proteasome inhibition prevented the marked reduction of H2A.Z due to sirtuin 1 overexpression. Prostate cancer cells exposed to epigenetic modifying drugs trichostatin A, alone or combined with 5-aza-2'-deoxycytidine, increased H2AFZ transcript, although with a concomitant decrease in protein levels. Conversely, SIRT1 transcript and protein levels increased after exposure. ChIP revealed an increase of activation marks within the TSS region for both genes. Remarkably, inhibition of sirtuin 1 with nicotinamide, increased H2A.Z levels, whereas activation of sirtuin 1 by resveratrol led to an abrupt decrease in H2A.Z. Finally, protein-ligation assay showed that exposure to epigenetic modifying drugs fostered the interaction between sirtuin 1 and H2A.Z. We concluded that sirtuin 1 and H2A.Z deregulation in prostate cancer are reciprocally related. Epigenetic mechanisms, mostly histone post-translational modifications, are likely involved and impair sirtuin 1-mediated downregulation of H2A.Z via proteasome-mediated degradation. Epigenetic modifying drugs in conjunction with enzymatic modulators are able to restore the normal functions of sirtuin 1 and might constitute relevant tools for targeted therapy of prostate cancer patients.
- Published
- 2013
- Full Text
- View/download PDF
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