8 results on '"Barba, Cindy"'
Search Results
2. Somatic mutations in telomerase promoter counterbalance germline loss-of-function mutations
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Maryoung, Lindley, Yue, Yangbo, Young, Ashley, Newton, Chad A., Barba, Cindy, van Oers, Nicolai S.C., Wang, Richard C., and Garcia, Christine Kim
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Respiratory tract diseases -- Genetic aspects -- Risk factors -- Research ,Gene mutation -- Research ,Telomerase -- Research ,Health care industry - Abstract
Germline coding mutations in different telomere-related genes have been linked to autosomal-dominant familial pulmonary fibrosis. Individuals with these inherited mutations demonstrate incomplete penetrance of clinical phenotypes affecting the lung, blood, liver, skin, and other organs. Here, we describe the somatic acquisition of promoter mutations in telomerase reverse transcriptase (TERT) in blood leukocytes of approximately 5% of individuals with inherited loss-of-function coding mutations in TERT or poly(A)-specific ribonuclease (PARN), another gene linked to telomerase function. While these promoter mutations were initially identified as oncogenic drivers of cancer, individuals expressing the mutations have no history of cancer. Neither promoter mutation was found in population-based cohorts of similar or advanced age. The TERT promoter mutations were found more frequently in cis with the WT allele than the TERT coding sequence mutation. EBV-transformed lymphoblastoid B cell lines (LCLs) derived from subjects with TERT promoter mutations showed increased telomerase expression and activity compared with cell lines from family members with identical coding mutations. TERT promoter mutations resulted in an increased proliferation of LCLs and demonstrated positive selection over time. The persistence and recurrence of noncoding gain-of-function mutations in these cases suggests that telomerase activation is not only safely tolerated but also advantageous for clonal expansion., Introduction Telomerase is a reverse transcriptase that extends the length of repetitive DNA sequences at the ends of chromosomes (1). In contrast to normal tissue, in which telomerase activity is [...]
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- 2017
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3. miRNA-1 promotes acute myeloid leukemia cell pathogenesis through metabolic regulation.
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Ghazaryan, Arevik, Wallace, Jared A., Tang, William W., Barba, Cindy, Soh-Hyun Lee, Bauer, Kaylyn M., Nelson, Morgan C., Kim, Carissa N., Stubben, Chris, Voth, Warren P., Rao, Dinesh S., and O'Connell, Ryan M.
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ACUTE myeloid leukemia ,METABOLIC regulation ,GENE expression ,MYELOID cells ,FUMARATES - Abstract
Acute myeloid leukemia (AML) is a heterogeneous and deadly disease characterized by uncontrolled expansion of malignant blasts. Altered metabolism and dysregulated microRNA (miRNA) expression profiles are both characteristic of AML. However, there is a paucity of studies exploring how changes in the metabolic state of the leukemic cells regulate miRNA expression leading to altered cellular behavior. Here, we blocked pyruvate entry into mitochondria by deleting the Mitochondria Pyruvate Carrier (MPC1) gene in human AML cell lines, which decreased Oxidative Phosphorylation (OXPHOS). This metabolic shift also led to increased expression of miR-1 in the human AML cell lines tested. AML patient sample datasets showed that higher miR-1 expression correlates with reduced survival. Transcriptional and metabolic profiling of miR-1 overexpressing AML cells revealed that miR-1 increased OXPHOS, along with key metabolites that fuel the TCA cycle such as glutamine and fumaric acid. Inhibition of glutaminolysis decreased OXPHOS in miR-1 overexpressing MV4-11 cells, highlighting that miR-1 promotes OXPHOS through glutaminolysis. Finally, overexpression of miR-1 in AML cells exacerbated disease in a mouse xenograft model. Together, our work expands current knowledge within the field by uncovering novel connections between AML cell metabolism and miRNA expression that facilitates disease progression. Further, our work points to miR-1 as a potential new therapeutic target that may be used to disrupt AML cell metabolism and thus pathogenesis in the clinic. [ABSTRACT FROM AUTHOR]
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- 2023
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4. Interferon Gamma-Inducible NAMPT in Melanoma Cells Serves as a Mechanism of Resistance to Enhance Tumor Growth.
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Barba, Cindy, Ekiz, H. Atakan, Tang, William Weihao, Ghazaryan, Arevik, Hansen, Mason, Lee, Soh-Hyun, Voth, Warren Peter, and O'Connell, Ryan Michael
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BIOLOGICAL models , *IN vivo studies , *MELANOMA , *ANIMAL experimentation , *INTERFERONS , *GENE expression , *MOLECULAR biology , *TRANSFERASES , *CELL proliferation , *RESEARCH funding , *MICE - Abstract
Simple Summary: The tumor microenvironment is complex, with interacting immune and tumor cells. Immune cells release inflammatory cytokines, including interferons (IFNs), that drive tumor clearance. However, evidence suggests that tumor cells can also utilize IFNs to enhance growth and survival in certain cases. We demonstrate that interferon gamma (IFNγ) mediates the metabolic reprogramming of melanoma cells by inducing the essential NAD+ salvage pathway enzyme nicotinamide phosphoribosyltransferase (NAMPT) gene through STAT1 binding to the NAMPT locus. NAMPT is constitutively expressed in cells during normal homeostasis. However, melanoma cells have higher energetic demands and increased NAMPT. We show that IFNγ signaling upregulates NAMPT in melanoma cells, increasing cell proliferation and survival. Further, STAT1-inducible Nampt promotes melanoma growth in mice. We provide evidence that melanoma cells directly respond to IFNγ-activated STAT1 by increasing Nampt, which improves their fitness during tumor immunity. Elucidating mechanisms that regulate NAMPT expression can lead to enhanced therapeutic approaches with immunotherapies that utilize IFN signaling to improve patient outcomes. (1) Background: Immune cells infiltrate the tumor microenvironment and secrete inflammatory cytokines, including interferons (IFNs), to drive antitumor responses and promote tumor clearance. However, recent evidence suggests that sometimes, tumor cells can also harness IFNs to enhance growth and survival. The essential NAD+ salvage pathway enzyme nicotinamide phosphoribosyltransferase (NAMPT) gene is constitutively expressed in cells during normal homeostasis. However, melanoma cells have higher energetic demands and elevated NAMPT expression. We hypothesized that interferon gamma (IFNγ) regulates NAMPT in tumor cells as a mechanism of resistance that impedes the normal anti-tumorigenic effects of IFNγ. (2) Methods: Utilizing a variety of melanoma cells, mouse models, Crispr-Cas9, and molecular biology techniques, we explored the importance of IFNγ-inducible NAMPT during melanoma growth. (3) Results: We demonstrated that IFNγ mediates the metabolic reprogramming of melanoma cells by inducing Nampt through a Stat1 binding site in the Nampt gene, increasing cell proliferation and survival. Further, IFN/STAT1-inducible Nampt promotes melanoma in vivo. (4) Conclusions: We provided evidence that melanoma cells directly respond to IFNγ by increasing NAMPT levels, improving their fitness and growth in vivo (control n = 36, SBS KO n = 46). This discovery unveils a possible therapeutic target that may improve the efficacy of immunotherapies involving IFN responses in the clinic. [ABSTRACT FROM AUTHOR]
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- 2023
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5. miR-aculous new avenues for cancer immunotherapy.
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Tang, William W., Bauer, Kaylyn M., Barba, Cindy, Ekiz, Huseyin Atakan, and O’Connell, Ryan M.
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NON-coding RNA ,IMMUNOTHERAPY ,CYTOLOGY ,CANCER cells ,MICRORNA - Abstract
The rising toll of cancer globally necessitates ingenuity in early detection and therapy. In the last decade, the utilization of immune signatures and immunebased therapies has made significant progress in the clinic; however, clinical standards leave many current and future patients without options. Non-coding RNAs, specifically microRNAs, have been explored in pre-clinical contexts with tremendous success. MicroRNAs play indispensable roles in programming the interactions between immune and cancer cells, many of which are current or potential immunotherapy targets. MicroRNAs mechanistically control a network of target genes that can alter immune and cancer cell biology. These insights provide us with opportunities and tools that may complement and improve immunotherapies. In this review, we discuss immune and cancer cell–derived miRNAs that regulate cancer immunity and examine miRNAs as an integral part of cancer diagnosis, classification, and therapy. [ABSTRACT FROM AUTHOR]
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- 2022
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6. Actin Bundling Protein L-Plastin Regulates Megakaryocyte Membrane Rigidity and Platelet Spreading
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Guo, Li, Bhatlekar, Seema, Jacob, Shancy P, Manne, Bhanu Kanth, Kolawole, Elizabeth, Tugolukova, Emilia A, Guo, Siqi, Kosaka, Yasuhiro, Barba, Cindy, Evavold, Brian, Morley, Sharon Celesty, Campbell, Robert A., and Bray, Paul F.
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- 2022
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7. Obesity Modulates Intestinal Intraepithelial T Cell Persistence, CD103 and CCR9 Expression, and Outcome in Dextran Sulfate Sodium-Induced Colitis.
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Park, Christa, Cheung, Kitty P., Limon, Natalie, Costanzo, Anne, Barba, Cindy, Miranda, Nadia, Gargas, Shannon, Johnson, Andrew M. F., Olefsky, Jerrold M., and Jameson, Julie M.
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DEXTRAN sulfate , *T cells , *COLITIS , *OBESITY , *CHILDHOOD obesity - Abstract
Obesity impacts over 30% of the United States population, resulting in a wide array of complications. Included among these is the deterioration of the intestinal barrier, which has been implicated in type 2 diabetes and susceptibility to bacterial transepithelial migration. The intestinal epithelium is maintained by αβ and γδ intraepithelial T lymphocytes, which migrate along the epithelia, support epithelial homeostasis, and protect from infection. In this study, we investigate how obesity impacts intraepithelial lymphocyte (IEL) persistence and function in intestinal homeostasis and repair. Mice were fed a high-fat diet to induce obesity and to study immunomodulation in the intestine. There is a striking reduction in αβ and γδ IEL persistence as obesity progresses with a different mechanism in αβ versus γδ IEL populations. CD4+ and CD4+CD8+ αβ intraepithelial T lymphocytes exhibit reduced homeostatic proliferation in obesity, whereas both αβ and γδ IELs downregulate CD103 and CCR9. The reduction in intraepithelial T lymphocytes occurs within 7 wk of high-fat diet administration and is not dependent on chronic inflammation via TNF-α. Young mice administered a high-fat diet upon weaning exhibit the most dramatic phenotype, showing that childhood obesity has consequences on intestinal IEL seeding. Together, this dysfunction in the intestinal epithelium renders obese mice more susceptible to dextran sulfate sodium--induced colitis. Diet-induced weight loss restores IEL number and CD103/CCR9 expression and improves outcome in colitis. Together, these data confirm that obesity has immunomodulatory consequences in intestinal tissues that can be improved with weight loss. [ABSTRACT FROM AUTHOR]
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- 2019
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8. Actin-bundling protein L-plastin promotes megakaryocyte rigidity and dampens proplatelet formation.
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Guo L, Jacob S, Manne BK, Kolawole EM, Guo S, Wang X, Murray D, Tugolukova EA, Portier I, Kosaka Y, Barba C, Rondina MT, Evavold B, Morley C, Bhatlekar S, and Bray PF
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- Humans, Microfilament Proteins genetics, Microfilament Proteins metabolism, Membrane Glycoproteins metabolism, Phosphoproteins metabolism, Actins metabolism, Megakaryocytes metabolism
- Published
- 2024
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- View/download PDF
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