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342 results on '"Barbosa, Mário A."'

6. Author Correction: Ionizing radiation modulates human macrophages towards a pro-inflammatory phenotype preserving their pro-invasive and pro-angiogenic capacities

Author

Teresa Pinto, Ana, Laranjeiro Pinto, Marta, Patrícia Cardoso, Ana, Monteiro, Cátia, Teixeira Pinto, Marta, Filipe Maia, André, Castro, Patrícia, Figueira, Rita, Monteiro, Armanda, Marques, Margarida, Mareel, Marc, dos Santos, Susana Gomes, Seruca, Raquel, Adolfo Barbosa, Mário, Rocha, Sónia, and José Oliveira, Maria

Published
2022
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12. Engineering Anisotropic Cell Models: Development of Collagen Hydrogel Scaffolds with Magneto‐Responsive PEG Microgels for Tissue Engineering Applications.

Author

Castro, Ana L., Vedaraman, Sitara, Haraszti, Tamás, Barbosa, Mário A., Gonçalves, Raquel M., and De Laporte, Laura

Subjects
MICROGELS, RHEOLOGY, POLYETHYLENE glycol, COLLAGEN, HYDROGELS, GELATION, TISSUE engineering, RHEOLOGY (Biology)
Abstract

Mimicking tissue‐oriented organization in vitro has been extensively studied in recent years, using both natural and synthetic materials in combination with external magnetic fields to establish anisotropic conditions. Here, a new combination between magneto‐responsive anisometric PEG microgels and collagen hydrogels is explored to establish anisotropic in vitro models. Different sizes of PEG microgels are tested to assess the impact of both width and aspect ratio on the formation and alignment of collagen hydrogels. Results show that the key properties of collagen hydrogels, regarding fibrillogenesis, rheological properties, and fiber diameter are kept consistent upon the combination with PEG microgels. Furthermore, partial collagen fiber alignment is observed when larger (width 10 µm) PEG microgels are employed and magnetically aligned. In vitro studies show cell alignment within the anisotropic collagen hydrogels from the first day in culture. Interestingly, PEG microgels with higher width and length tend to induce less hydrogel contraction even after 7 days in culture. The results demonstrate the ability to establish a 3D unidirectional collagen hydrogel by magnetically aligning anisometric microgels during the gelation process, which can be promising for different tissue engineering applications. [ABSTRACT FROM AUTHOR]

Published
2024
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26. Combining inflammatory miRNA molecules as diagnostic biomarkers for depression: a clinical study.

Author

Paulo Brás, João, Pinto, Sara, von Doellinger, Orlando, Prata, Joana, Coelho, Rui, Adolfo Barbosa, Mário, Inês Almeida, Maria, and Gomes Santos, Susana

Subjects
MONONUCLEAR leukocytes, GENE expression, INFLAMMATORY mediators, RECEIVER operating characteristic curves, MENTAL depression
Abstract

Background: Inflammation has been implicated in core features of depression pathophysiology and treatment resistance. Therefore, new challenges in the discovery of inflammatory mediators implicated in depression have emerged. MicroRNAs (miRNAs) have been found aberrantly expressed in several pathologies, increasing their potential as biomarkers and therapeutical targets. In this study, the aim was to assess the changes and biomarker potential of inflammation-related miRNAs in depression patients. Methods: Depression diagnosis was performed according to the Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition (DSM-5). 40 healthy controls and 32 depression patients were included in the study. The levels of inflammatory cytokines were measured in plasma, and expression levels of cytokines and inflammation-related miRNAs were evaluated in peripheral blood mononuclear cells (PBMCs). Results: Depression patients were found to have a pro-inflammatory profile in plasma, with significantly higher levels of TNF-α and CCL2 compared with controls. In PBMCs of depression patients, TNF-α and IL-6 expression levels were significantly up and downregulated, respectively. Moreover, miR-342 levels were found upregulated, while miR-146a and miR-155 were significantly downregulated. miR-342 expression levels were positively correlated with TNF-α. Importantly, when analyzed as a diagnostic panel, receiver operating characteristics (ROC) analysis of miR-342, miR-146a, miR-155 in combination, showed to be highly specific and sensitive in distinguishing between depression patients and healthy controls. Conclusion: In summary, these findings suggest that inflammation-related miRNAs are aberrantly expressed in depression patients. Moreover, we show evidences on the potential of the combination of dysregulated miRNAs as a powerful diagnostic tool for depression. [ABSTRACT FROM AUTHOR]

Published
2023
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27. Alterations of bovine nucleus pulposus cells with aging.

Author

Molinos, Maria, Fiordalisi, Morena F., Caldeira, Joana, Almeida, Catarina R., Barbosa, Mário A., and Gonçalves, Raquel M.

Subjects
NUCLEUS pulposus, PROTEIN expression, INTERVERTEBRAL disk, LUMBAR pain, CELL morphology
Abstract

Aging is one of the major etiological factors driving intervertebral disc (IVD) degeneration, the main cause of low back pain. The nucleus pulposus (NP) includes a heterogeneous cell population, which is still poorly characterized. Here, we aimed to uncover main alterations in NP cells with aging. For that, bovine coccygeal discs from young (12 months) and old (10–16 years old) animals were dissected and primary NP cells were isolated. Gene expression and proteomics of fresh NP cells were performed. NP cells were labelled with propidium iodide and analysed by flow cytometry for the expression of CD29, CD44, CD45, CD146, GD2, Tie2, CD34 and Stro‐1. Morphological cell features were also dissected by imaging flow cytometry. Elder NP cells (up‐regulated bIL‐6 and bMMP1 gene expression) presented lower percentages of CD29+, CD44+, CD45+ and Tie2+ cells compared with young NP cells (upregulated bIL‐8, bCOL2A1 and bACAN gene expression), while GD2, CD146, Stro‐1 and CD34 expression were maintained with age. NP cellulome showed an upregulation of proteins related to endoplasmic reticulum (ER) and melanosome independently of age, whereas proteins upregulated in elder NP cells were also associated with glycosylation and disulfide bonds. Flow cytometry analysis of NP cells disclosed the existence of 4 subpopulations with distinct auto‐fluorescence and size with different dynamics along aging. Regarding cell morphology, aging increases NP cell area, diameter and vesicles. These results contribute to a better understanding of NP cells aging and highlighting potential anti‐aging targets that can help to mitigate age‐related disc disease. [ABSTRACT FROM AUTHOR]

Published
2023
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33. The Paramount Importance of Renal Dysfunction in Heart Failure Prognosis - Results from the REFERENCE study.

Author

Barbosa, Mário, Matos, Andreia, Bicho, Manuel, and Menezes Falcão, Luiz

Subjects
CARDIO-renal syndrome, HEART failure, KIDNEY diseases, PROPORTIONAL hazards models, GLOMERULAR filtration rate, UNIVARIATE analysis, CREATININE, HEART failure patients, PROGNOSIS
Abstract

Copyright of Galicia Clínica is the property of Sociedad Gallega de Medicina Interna and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2023
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38. WCN24-1326 ANTIHYPERTENSIVE TREATMENT AND SODIUM CONSUMPTION AS CONFOUNDING FACTORS IN THE ALDOSTERONE-RENIN RATIO, RENIN, AND ALDOSTERONE LEVELS IN AFRO-COLOMBIAN APPARENT RESISTANT HYPERTENSIVE PATIENTS: A PRI

Author

Duran, Carlos, Bustamante, Melissa, Useche Henao, Elena Maria, Barbosa, Mario, Moncayo, Paula Andrea, Meza, Sebastian, Bolaños, Juan sebastian, Schweineberg, Johanna, Posada, Juan Gillermo, Mesa, Liliana, Rivas, Adriana, Sandoval, Lina Marcela, Triviño, Jacobo, Quintero, Juan David, and Zapata, Juan Sebastian

Published
2024
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50. Ionizing radiation modulates human macrophages towards a pro-inflammatory phenotype preserving their pro-invasive and pro-angiogenic capacities

Author

Teresa Pinto, Ana, Laranjeiro Pinto, Marta, Patrícia Cardoso, Ana, Monteiro, Cátia, Teixeira Pinto, Marta, Filipe Maia, André, Castro, Patrícia, Figueira, Rita, Monteiro, Armanda, Marques, Margarida, Mareel, Marc, dos Santos, Susana Gomes, Seruca, Raquel, Adolfo Barbosa, Mário, Rocha, Sónia, and José Oliveira, Maria

Published
2016
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