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7. The IL-8-CXCR1/2 axis contributes to diabetic kidney disease

Author

Loretelli, Cristian, Rocchio, Francesca, D'Addio, Francesca, Ben Nasr, Moufida, Castillo-Leon, Eduardo, Dellepiane, Sergio, Vergani, Andrea, Abdelsalam, Ahmed, Assi, Emma, Maestroni, Anna, Usuelli, Vera, Bassi, Roberto, Pastore, Ida, Yang, Jun, El Essawy, Basset, Elased, Khalid M., Fadini, Gian Paolo, Ippolito, Elio, Seelam, Andy Joe, Pezzolesi, Marcus, Corradi, Domenico, Zuccotti, Gian Vincenzo, Gallieni, Maurizio, Allegretti, Marcello, Niewczas, Monika Anna, and Fiorina, Paolo

Published
2021
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12. Embryonic stem cell extracts improve wound healing in diabetic mice

Author

Loretelli, Cristian, Ben Nasr, Moufida, Giatsidis, Giorgio, Bassi, Roberto, Lancerotto, Luca, D’Addio, Francesca, Valderrama-Vasquez, Alessandro, Scherer, Saja Sandra, Salvatore, Luca, Madaghiele, Marta, Abdelsalam, Ahmed, Ippolito, Elio, Assi, Emma, Usuelli, Vera, El Essawy, Basset, Sannino, Alessandro, Pietramaggiori, Giorgio, Zuccotti, Gian Vincenzo, Orgill, Dennis Paul, and Fiorina, Paolo

Published
2020
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20. Molecular mechanisms of light harvesting in the minor antenna CP29 in near-native membrane lipidic environment.

Author

Sardar, Samim, Caferri, Roberto, Camargo, Franco V. A., Pamos Serrano, Javier, Ghezzi, Alberto, Capaldi, Stefano, Dall'Osto, Luca, Bassi, Roberto, D'Andrea, Cosimo, and Cerullo, Giulio

Subjects
CHLOROPHYLL spectra, BILAYER lipid membranes, XANTHOPHYLLS, CARRIER proteins, ANTENNAS (Electronics), ENERGY transfer, PHOTOSYSTEMS
Abstract

CP29, a chlorophyll a/b-xanthophyll binding protein, bridges energy transfer between the major LHCII antenna complexes and photosystem II reaction centers. It hosts one of the two identified quenching sites, making it crucial for regulated photoprotection mechanisms. Until now, the photophysics of CP29 has been studied on the purified protein in detergent solutions since spectrally overlapping signals affect in vivo measurements. However, the protein in detergent assumes non-native conformations compared to its physiological state in the thylakoid membrane. Here, we report a detailed photophysical study on CP29 inserted in discoidal lipid bilayers, known as nanodiscs, which mimic the native membrane environment. Using picosecond time-resolved fluorescence and femtosecond transient absorption (TA), we observed shortening of the Chl fluorescence lifetime with a decrease of the carotenoid triplet formation yield for CP29 in nanodiscs as compared to the protein in detergent. Global analysis of TA data suggests a 1Chl* quenching mechanism dependent on excitation energy transfer to a carotenoid dark state, likely the proposed S*, which is believed to be formed due to a carotenoid conformational change affecting the S1 state. We suggest that the accessibility of the S* state in different local environments plays a key role in determining the quenching of Chl excited states. In vivo, non-photochemical quenching is activated by de-epoxidation of violaxanthin into zeaxanthin. CP29-zeaxanthin in nanodiscs further shortens the Chl lifetime, which underlines the critical role of zeaxanthin in modulating photoprotection activity. [ABSTRACT FROM AUTHOR]

Published
2022
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25. A systems-wide understanding of photosynthetic acclimation in algae and higher plants

Author

Moejes, Fiona Wanjiku, Matuszyńska, Anna, Adhikari, Kailash, Bassi, Roberto, Cariti, Federica, Cogne, Guillaume, Dikaios, Ioannis, Falciatore, Angela, Finazzi, Giovanni, Flori, Serena, Goldschmidt-Clermont, Michel, Magni, Stefano, Maguire, Julie, Le Monnier, Adeline, Müller, Kathrin, Poolman, Mark, Singh, Dipali, Spelberg, Stephanie, Stella, Giulio Rocco, Succurro, Antonella, Taddei, Lucilla, Urbain, Brieuc, Villanova, Valeria, Zabke, Claudia, and Ebenhöh, Oliver

Published
2017

28. Look for methods, not conclusions

Author

Bassi, Roberto, Bucci, Enrico M., Calogero, Raffaele A., Carninci, Piero, Ciliberto, Gennaro, Conte, Pellegrino, De Luca, Michele, Corbellini, Gilberto, Giordano, Antonio, Marchionni, Luigi, Massaro Giordano, Giacomina, Parini, Angelo, and Sbardella, Gianluca

Published
2019
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32. P2X7R mutation disrupts the NLRP3-mediated Th program and predicts poor cardiac allograft outcomes

Author

D'Addio, Francesca, Vergani, Andrea, Potena, Luciano, Maestroni, Anna, Usuelli, Vera, Nasr, Moufida Ben, Bassi, Roberto, Tezza, Sara, Dellepiane, Sergio, Essawy, Basset El, Iascone, Maria, Iacovoni, Attilio, Borgese, Laura, Liu, Kaifeng, Visner, Gary, Dhe-Paganon, Sirano, Corradi, Domenico, Abdi, Reza, Starling, Randall C., Folli, Franco, Zuccotti, Gian Vincenzo, Sayegh, Mohamed H., Heeger, Peter S., Chandraker, Anil, Grigioni, Francesco, and Fiorina, Paolo

Subjects
Heart transplantation -- Usage -- Complications and side effects, Gene mutation -- Research, Cardiac patients -- Health aspects, Transcription factors -- Analysis, T cells -- Research, Health care industry
Abstract

Purinergic receptor-7 (P2X7R) signaling controls Th17 and Th1 generation/differentiation, while NOD-like receptor P3 (NLRP3) acts as a Th2 transcriptional factor. Here, we demonstrated the existence of a P2X7R/NLRP3 pathway in T cells that is dysregulated by a P2X7R intracellular region loss-of-function mutation, leading to NLRP3 displacement and to excessive Th17 generation due to abrogation of the NLRP3-mediated Th2 program. This ultimately resulted in poor outcomes in cardiac-transplanted patients carrying the mutant allele, who showed abnormal Th17 generation. Transient NLRP3 silencing in nonmutant T cells or overexpression in mutant T cells normalized the Th profile. Interestingly, IL-17 blockade reduced Th17 skewing of human T cells in vitro and abrogated the severe allograft vasculopathy and abnormal Th17 generation observed in preclinical models in which P2X7R was genetically deleted. This P2X7R intracellular region mutation thus impaired the modulatory effects of P2X7R on NLRP3 expression and function in T cells and led to NLRP3 dysregulation and Th17 skewing, delineating a high-risk group of cardiac-transplanted patients who may benefit from personalized therapy., Introduction Purinergic receptor-7 (P2X7R), primarily expressed on lymphocytes, senses adenosine 5'-triphosphate (ATP) (1) released extracellularly during cell damage (2, 3) and has been shown to regulate T cell activation (4-6). [...]

Published
2018
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37. A paler shade of green: engineering cellular chlorophyll content to enhance photosynthesis in crowded environments.

Author

Cutolo, Edoardo Andrea, Guardini, Zeno, Dall'Osto, Luca, and Bassi, Roberto

Subjects
SUSTAINABLE engineering, CHLOROPLAST membranes, CHLOROPHYLL, PLANT breeding, PHOTOSYNTHESIS, PHOTOSYNTHETIC pigments, PLANT pigments
Abstract

Summary: In natural ecosystems, plants compete for space, nutrients and light. The optically dense canopies limit the penetration of photosynthetically active radiation and light often becomes a growth‐limiting factor for the understory. The reduced availability of photons in the lower leaf layers is also a major constraint for yield potential in canopies of crop monocultures. Traditionally, crop breeding has selected traits related to plant architecture and nutrient assimilation rather than light use efficiency. Leaf optical density is primarily determined by tissue morphology and by the foliar concentration of photosynthetic pigments (chlorophylls and carotenoids). Most pigment molecules are bound to light‐harvesting antenna proteins in the chloroplast thylakoid membranes, where they serve photon capture and excitation energy transfer toward reaction centers of photosystems. Engineering the abundance and composition of antenna proteins has been suggested as a strategy to improve light distribution within canopies and reduce the gap between theoretical and field productivity. Since the assembly of the photosynthetic antennas relies on several coordinated biological processes, many genetic targets are available for modulating cellular chlorophyll levels. In this review, we outline the rationale behind the advantages of developing pale green phenotypes and describe possible approaches toward engineering light‐harvesting systems. [ABSTRACT FROM AUTHOR]

Published
2023
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46. Ultrabroadband two-dimensional electronic spectroscopy reveals energy flow pathways in LHCII across the visible spectrum

Author

Son Minjung, Pinnola Alberta, Bassi Roberto, and Schlau-Cohen Gabriela S.

Subjects
Physics, QC1-999
Abstract

We utilise ultrabroadband two-dimensional electronic spectroscopy to map out pathways of energy flow in LHCII across the entire visible region. In addition to the well-established, low-lying chlorophyll Qy bands, our results reveal additional pathways of energy relaxation on the higher-lying excited states involving the S2 energy levels of carotenoids, including ultrafast carotenoid-to-chlorophyll energy transfer on 90-150 fs timescales.

Published
2019
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