28 results on '"Belligoli, Anna"'
Search Results
2. Psychological predictors of poor weight loss following LSG: relevance of general psychopathology and impulsivity
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Testa, Giulia, Granero, Roser, Siragusa, Cinzia, Belligoli, Anna, Sanna, Marta, Rusconi, Maria Luisa, Angeli, Paolo, Vettor, Roberto, Foletto, Mirto, Busetto, Luca, Fernández-Aranda, Fernando, and Schiff, Sami
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- 2020
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3. Predicting Responses to Bariatric and Metabolic Surgery
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Belligoli, Anna, Bettini, Silvia, Segato, Gianni, and Busetto, Luca
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- 2020
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4. Diet approach before and after bariatric surgery
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Bettini, Silvia, Belligoli, Anna, Fabris, Roberto, and Busetto, Luca
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- 2020
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5. Bariatric surgery: Is a matter of cutting calories or cutting metabolic regulators?
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Belligoli, Anna, Bettini, Silvia, and Busetto, Luca
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- 2019
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6. Modifications of Resting Energy Expenditure After Sleeve Gastrectomy
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Bettini, Silvia, Bordigato, Emanuel, Fabris, Roberto, Serra, Roberto, Dal Pra’, Chiara, Belligoli, Anna, Sanna, Marta, Compagnin, Chiara, Foletto, Mirto, Prevedello, Luca, Fioretto, Paola, Vettor, Roberto, and Busetto, Luca
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- 2018
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7. Characterization of subcutaneous and omental adipose tissue in patients with obesity and with different degrees of glucose impairment
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Belligoli, Anna, Compagnin, Chiara, Sanna, Marta, Favaretto, Francesca, Fabris, Roberto, Busetto, Luca, Foletto, Mirto, Dal Prà, Chiara, Serra, Roberto, Prevedello, Luca, Da Re, Chiara, Bardini, Romeo, Mescoli, Claudia, Rugge, Massimo, Fioretto, Paola, Conci, Scilla, Bettini, Silvia, Milan, Gabriella, and Vettor, Roberto
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- 2019
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8. Correction to: Diet approach before and after bariatric surgery
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Bettini, Silvia, Belligoli, Anna, Fabris, Roberto, and Busetto, Luca
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- 2020
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9. Functional Evaluation and VO2-kinetics in Obese Patients Before and After Sleeve Gastrectomy: 1527 Board #289 May 30 10:30 AM - 12:00 PM
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Neunhaeuserer, Daniel, Ortolan, Sara, Patti, Alessandro, Gasperetti, Andrea, Savalla, Francesco, Battista, Francesca, Gobbo, Stefano, Bettini, Silvia, Belligoli, Anna, and Ermolao, Andrea
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- 2019
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10. Incidence and Predictors of Hypoglycemia 1 Year After Laparoscopic Sleeve Gastrectomy
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Belligoli, Anna, Sanna, Marta, Serra, Roberto, Fabris, Roberto, Pra’, Chiara Dal, Conci, Scilla, Fioretto, Paola, Prevedello, Luca, Foletto, Mirto, Vettor, Roberto, and Busetto, Luca
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- 2017
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11. Cardiorespiratory function and VO2 kinetics after sleeve gastrectomy: a follow-up analysis
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Neunhaeuserer Daniel, Belligoli Anna, Vettor Roberto, Busetto Luca, Campi Cristina, Savalla Francesco, Foletto Mirto, Ermolao Andrea, Bergamin Marco, Rami Anna, Gasperetti Andrea, and Gobbo Stefano
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Bariatric surgery ,medicine.medical_specialty ,Sleeve gastrectomy ,Oxygen uptake kinetics ,business.industry ,medicine.medical_treatment ,Cardiopulmonary exercise test ,Cardiorespiratory fitness ,030204 cardiovascular system & hematology ,Muscle mass ,Oxygen uptake ,Peripheral ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Emergency Medicine ,Internal Medicine ,Cardiology ,Medicine ,In patient ,030212 general & internal medicine ,business ,Vo2 kinetics ,Aerobic capacity - Abstract
Although poor cardiorespiratory function is a powerful predictor of mortality in patients with obesity, functional evaluation has been given little attention after laparoscopic sleeve gastrectomy (LSG). The aim of this study was thus to investigate the impact of LSG on functional capacity analysing a shorter and longer post-surgical follow-up. In this observational study, 24 patients with severe obesity (BMI 44.04 ± 5.84 kg/m2, age 47.0 ± 9.0 years) performed cardiopulmonary exercise testing 7 months pre-, 6 and 16 months post-LSG. Exercise capacity, oxygen uptake and VO2 kinetics were analysed during treadmill exercise testing. Although patients showed an improved peak work capacity after LSG, parameters of maximal absolute aerobic capacity demonstrated a significant decrease, which was confirmed also in the follow-up analysis. Furthermore, the time constant Tau (τ) of the fundamental component of VO2 kinetics was altered by LSG, however, within the 16 months of follow-up it returned towards baseline values. It can thus be concluded that LSG improved peak work capacity along with a lower maximal absolute aerobic capacity, which might be due to a loss of muscle mass and potentially impaired peripheral oxidative muscle metabolism. However, VO2 kinetics might suggest an initial restoration of peripheral oxidative muscle capacity after a longer follow-up.
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- 2020
12. Metabolic Response to Submaximal and Maximal Exercise in People with Severe Obesity, Prediabetes, and Diabetes.
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Battista, Francesca, Belligoli, Anna, Neunhaeuserer, Daniel, Gasperetti, Andrea, Bettini, Silvia, Compagnin, Chiara, Marchese, Riccardo, Quinto, Giulia, Bergamin, Marco, Vettor, Roberto, Busetto, Luca, and Ermolao, Andrea
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EXERCISE intensity ,EXERCISE tests ,PREDIABETIC state ,TYPE 2 diabetes ,GLYCEMIC control ,BODY mass index - Abstract
Introduction: Metabolic adaptations to maximal physical exercise in people with obesity (PwO) are scarcely described. This cross-sectional study evaluates the metabolic response to exercise via the respiratory exchange ratio (RER) in PwO and different degrees of glycemic control. Methods: Eighty-five PwO (body mass index 46.0 [39.0–54.0] kg/m
2 ), that is, 32 normoglycemic (Ob-N), 25 prediabetic (Ob-preDM), and 28 diabetic (Ob-T2DM) subjects and 18 healthy subjects performed an incremental, maximal cardiopulmonary exercise test. The RER was measured at rest (RERrest) and at peak exercise (RERpeak). Results: RERpeak was significantly higher in healthy subjects than that in PwO. Among those, RERpeak was significantly higher in Ob-N than that in Ob-preDM and Ob-T2DM (1.20 [1.15–1.27] vs. 1.18 [1.10–1.22] p = 0.04 and vs. 1.14 [1.10–1.18] p < 0.001, respectively). Accordingly, ΔRER (RERpeak-RERrest) was lower in Ob-preDM and Ob-T2DM than that in Ob-N (0.32 [0.26–0.39] p = 0.04 and 0.29 [0.24–0.36] p < 0.001 vs. 0.38 [0.32–0.43], respectively), while no significant difference was found in ΔRER between Ob-preDM and Ob-T2DM and not even between Ob-N and healthy subjects. Moreover, ΔRER in PwO correlated with glucose area under curve (p = 0.002). Conclusions: PwO demonstrate restricted metabolic response during maximal exercise. Particularly, those with prediabetes already show metabolic inflexibility during exercise, similarly to those with type 2 diabetes. These findings also suggest a potential role of cardiopulmonary exercise testing in detecting early metabolic alterations in PwO. [ABSTRACT FROM AUTHOR]- Published
- 2021
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13. Effect of Bariatric Surgery on Non-alcoholic fatty liver disease
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Bettini, Silvia, Bordigato, Emanuel, DAL PRA, Chiara, Serra, Roberto, Leandri, Sonia, Belligoli, Anna, Sanna, Marta, Foletto, Mirto, Prevedello, Luca, Vettor, Roberto, Busetto, Luca, Giorgio, Fassina, and Roberto, Fabris
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- 2017
14. Neurocognitive alterations in severe obese individuals: neurophysiological and behavioral measures
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Testa, Giulia, Amodio, Piero, Buongiorno, Federica, Rusconi, Maria Luisa, Belligoli, Anna, Sanna, Marta, Vettor, Roberto, Foletto, Mirto, and Schiff, Sami
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Settore M-PSI/02 - Psicobiologia e Psicologia Fisiologica - Published
- 2017
15. Cognitive control in severe obese individuals: an ERPs study
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Testa, Giulia, Amodio, Piero, Buongiorno, Federica, Rusconi, Maria Luisa, Belligoli, Anna, Sanna, Marta, Vettor, Roberto, Foletto, Mirto, and Schiff, Sami
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obesity ,Settore M-PSI/02 - Psicobiologia e Psicologia Fisiologica ,cognitive control ,ERP - Published
- 2017
16. Metabolic flexibility after bariatric surgery-induced weight loss: does diabetes impair a proper substrates utilization?
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Conci, Scilla, Bettini, Silvia, Bordigato, Emanuel, Sanna, Marta, Belligoli, Anna, Roberto, Fabris, Serra, Roberto, DAL PRA, Chiara, Foletto, Mirto, Prevedello, Luca, Vettor, Roberto, and Busetto, Luca
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- 2017
17. Adipose tissue and insulin secretion in the pathophysiology of obesity and its complications
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Belligoli, Anna
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Settore MED/09 - Medicina Interna ,Adipose Tissue, Obesity, hypoglycaemia, sleeve-gastrectomy ,diabetes ,bariatric surgery ,hypogliceamia ,obesità ,adipose tissue ,chirurgia bariatrica ,diabete ,MED/09 Medicina interna ,tessuto adiposo ,ipoglicemia obesity ,sleeve-gastrectomy ,obesità, diabete, tessuto adiposo, chirurgia bariatrica, ipoglicemia obesity, diabetes, adipose tissue, bariatric surgery, hypogliceamia ,Obesity ,hypoglycaemia - Abstract
Type 2 diabetis mellitus (T2DM) and obesity are global health care problems that are closely linked together. The precise mechanisms linking the two conditions remain unclear. Indeed, while the close relationship between T2DM and weight gain is well established, not all obese subjects are diabetic and this paradox is still unexplained. Impaired tissue perfusion has been proposed as one of the common metabolic defects, but little is known about adipose tissue (AT) microangiopathy and its possible role in T2DM. In animal models of obesity and diabetes, expanding AT microvasculature appears structurally altered and the angiogenetic potential of adipose derived stem cells impaired. Several studies, in humans, suggest that obesity leads to an impaired angiogenesis and AT hypoxia, inducing an inflammatory and a profibrotic response that plays a pivotal role in the pathogenesis of metabolic complications related to weight gain, first of all insulin resistance and diabetes. Moreover, from a pathophysiological point of view it is well established that dysfunctional visceral adipose tissue (VAT) is one of the major determinants of metabolic complications of obesity, while subcutaneous depots has been considered metabolically healthy. Nevertheless it could be hypothesized that in the progress of obesity through the metabolic impairment, SAT could become dysfunctional as VAT. On the basis of these data, we planned to study both subcutaneous and visceral adipose tissue in terms of adipocytes size, capillary density, adipose tissue stem cells (ASCs), endotelial precursor of AT and adipogenic potential, in obese subjects compared to lean subjects and in obese patients with a different glyceamic profile. We collected subcutaneous (SAT) and/or visceral (VAT) adipose tissue (AT) from 249 patients divided in 5 different groups: 18 lean normal weight and normoglycemic subjects (18.5 < BMI < 24,9 kg/m2) as control group, 68 normoglycemic obese subjects (ob N), 65 pre-diabetic obese subjects (ob pre-T2DM), 57 diabetic obese subjects (ob T2DM) and 41obese patients after underwent to a relevant weight loss (ob WL), corresponding to at least 10% of body weight. In different representative subgroups of these samples we performed: 1) immunohistochemical analysis to evaluate the morphometry of adipocyte and capillary density; 2) flow cytofluorimetric analysis of stromal vascular fraction (SVF) in order to quantify adipose tissue stem cells (ASCs), defined as CD45-CD34+CD31-, and endothelial precursors cells (EPs) defined as CD45-CD34+CD31+; 3) in vitro culture of ASCs obtained from SVF, in order to estimate the adipogenic potential in the different groups and different depot of AT; 4) gene expression profile by RT-Real Time PCR of PPRγ, Leptin, VEGFA, VEGF2, HIF1α to correlate their expression with previous findings. Our study confirm that obese AT is less vascularized than lean AT but T2DM does not represent an aggravating factor to the vascular reduction already present in obesity. On the contrary, T2DM and also prediabetic condition are able to further modify AT architecture, remodeling mature adipocyte size and adipogenic potential mediated by ASCs, importantly reducing AT hyperplastic growth capacity. Moreover our results allow us to assume that primum movens in development of T2DM must be searched in AT architecture and that both depots, SAT and VAT, play a pivotal role in the development of this disease. Furthermore, considering the continuous increase in bariatric procedures to treat both weigh gain and associated co-morbidities, we plan to evaluate the effects of laparoscopic sleeve gastrectomy (LSG) after one year. Indeed, whereas the beneficial effects of this bariatric procedure are well known, side effects are lesser known. In particular, postprandial hypoglycaemia is a well described side effect after RYGB, but few data are available for LSG.. We enrolled a total of 197 consecutive non-diabetic morbidly obese who underwent to LSG in our Center for the Study and the Integrated Treatment of Obesity (Ce.S.I.T.O.). All patients were studied 12 months before and after LSG and, anthropometrics parameters, medical history, clinical examination, complete blood count and complete metabolic panel including a 3- hour OGTT, were collected. One year after LSG, all patients had a significant reduction in weight and BMI, a significant improvement in glucose and insulin profile, and a significant decrease in inflammatory markers. We found an high incidence of severe hypoglycaemia (32,8%) after a provocative test (OGTT). Patients with hypoglycaemic events had a lower weight and BMI and a greater %EBML after LSG. compared to patients without hypoglycemic events. Hypoglycaemia was more frequent in patients having lower age, lower fasting blood glucose levels and higher triglycerides levels before LSG.
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- 2016
18. In silico prediction of blood cholesterol levels from genotype data.
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Reggiani, Francesco, Carraro, Marco, Belligoli, Anna, Sanna, Marta, dal Prà, Chiara, Favaretto, Francesca, Ferrari, Carlo, Vettor, Roberto, and Tosatto, Silvio C. E.
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BLOOD cholesterol ,SIMULATION methods & models ,CHOLESTEROL metabolism ,GENOTYPES ,MACHINE performance - Abstract
In this work we present a framework for blood cholesterol levels prediction from genotype data. The predictor is based on an algorithm for cholesterol metabolism simulation available in literature, implemented and optimized by our group in the R language. The main weakness of the former simulation algorithm was the need of experimental data to simulate mutations in genes altering the cholesterol metabolism. This caveat strongly limited the application of the model in the clinical practice. In this work we present how this limitation could be bypassed thanks to an optimization of model parameters based on patient cholesterol levels retrieved from literature. Prediction performance has been assessed taking into consideration several scoring indices currently used for performance evaluation of machine learning methods. Our assessment shows how the optimization phase improved model performance, compared to the original version available in literature. [ABSTRACT FROM AUTHOR]
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- 2020
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19. Resting Energy Expenditure, Insulin Resistance and UCP1 Expression in Human Subcutaneous and Visceral Adipose Tissue of Patients With Obesity.
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Bettini, Silvia, Favaretto, Francesca, Compagnin, Chiara, Belligoli, Anna, Sanna, Marta, Fabris, Roberto, Serra, Roberto, Dal Prà, Chiara, Prevedello, Luca, Foletto, Mirto, Vettor, Roberto, Milan, Gabriella, and Busetto, Luca
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ADIPOSE tissues ,INSULIN resistance - Abstract
Determinants of resting energy expenditure (REE) in humans are still under investigation, especially the association with insulin resistance. Brown adipose tissue (AT) regulates energy expenditure through the activity of the uncoupling protein 1 (UCP1). White AT browning is the process by which some adipocytes within AT depots acquire properties of brown adipocytes ("brite" adipocytes) and it correlates with metabolic improvement. We analyzed determinants of REE in patients with obesity and assessed UCP1 expression as a "brite" marker in abdominal subcutaneous AT (SAT) and visceral omental AT (VAT). Clinical data, REE, free fat mass (FFM), and fat mass (FM) were determined in 209 patients with obesity. UCP1 , PPARG coactivator 1 alpha (PPARGC1A), transcription factor A, mitochondrial (TFAM), T-box transcription factor 1 (TBX1), and solute carrier family 27 member 1 (SLC27A1) expression was assayed in SAT and VAT samples, obtained during sleeve gastrectomy from 62 patients with obesity. REE and body composition data were also available for a subgroup of 35 of whom. In 209 patients with obesity a multiple regression model was computed with REE as the dependent variable and sex, waist, FFM, FM, homeostasis model assessment-insulin resistance (HOMA), interleukin-6 and High Density Lipoprotein-cholesterol as the independent variables. Only FFM, FM and HOMA were independently correlated with REE (r = 0.787, AdjRsqr = 0.602). In each patient VAT displayed a higher UCP1, PPARGC1A, TFAM, TBX1 , and SLC27A1 expression than SAT and UCP1 expression in VAT (UCP1 -VAT) correlated with Body Mass Index (BMI) (r = 0.287, p < 0.05). Introducing UCP1 -VAT in the multivariate model, we showed that FFM, HOMA, interleukin-6, High Density Lipoprotein-cholesterol, and UCP1 -VAT were independent factors correlated with REE (r = 0.736, AdjRsqr = 0.612). We confirmed that REE correlates with FFM, FM and HOMA in a large cohort of patients. Our results clearly showed that UCP1 -VAT expression was significantly increased in severe human obesity (BMI > 50 kg/m
2 ) and that it behaved as an independent predictor of REE. Lastly, we suggest that an increased REE and browning in metabolically complicated severe obesity could represent an effort to counteract further weight gain. [ABSTRACT FROM AUTHOR]- Published
- 2019
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20. SCCA-IgM as a Potential Biomarker of Non-Alcoholic Fatty Liver Disease in Patients with Obesity, Prediabetes and Diabetes Undergoing Sleeve Gastrectomy.
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Bettini, Silvia, Bordigato, Emanuel, Milan, Gabriella, Dal Pra', Chiara, Favaretto, Francesca, Belligoli, Anna, Sanna, Marta, Serra, Roberto, Foletto, Mirto, Prevedello, Luca, Busetto, Luca, Fassina, Giorgio, Vettor, Roberto, and Fabris, Roberto
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FATTY liver ,OVERWEIGHT persons ,LIVER histology ,SLEEVE gastrectomy ,PREDIABETIC state ,THERAPEUTICS ,WEIGHT loss - Abstract
Background: Non-alcoholic fatty liver disease (NAFLD) has a high prevalence in obesity and its presence should be screened. Laparoscopic sleeve gastrectomy (LSG) is an effective treatment for obesity, but its effects on NAFLD are still to be firmly established. The diagnosis of non-alcoholic steatohepatitis (NASH) is currently performed by liver biopsy, a costly and invasive procedure. Squamous cell carcinoma antigen-IgM (SCCA-IgM) is a biomarker of viral hepatitis to hepatocellular carcinoma development and its role in NAFLD to NASH progression has not yet been investigated. Objective: The aim of this study was to evaluate SCCA-IgM as a non-invasive biomarker of NAFLD/NASH in patients with different degrees of metabolic-complicated obesity before and after LSG. Method: Fifty-six patients with obesity were studied before and 12 months after LSG; anthropometric, biochemical, clinical, and imaging data were collected. Results: At baseline steatosis was strongly associated with the glycaemic profile (p = 0.016) and was already present in prediabetic patients with obesity (82%). Only 3 patients had an SCCA-IgM level above the normal cut-off. SCCA-IgM titre did not change according to glycaemic profile or steatosis. Metabolic and inflammatory factors and transaminases significantly reduced after LSG-induced weight loss, except for SCCA-IgM. The ALT/AST ratio decreased post-LSG correlated with BMI (r = 0.297, p = 0.031), insulin (r = 0.354, p = 0.014), and triglycerides (r = 0.355, p = 0.009) reduction. Conclusions: Our results confirm the tight link between NAFLD and metabolic complications, suggesting prediabetes as a new risk factor of steatosis. SCCA-IgM does not seem to have a role in the identification and prognosis of NAFLD. [ABSTRACT FROM AUTHOR]
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- 2019
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21. Hypoglycaemia one-year after laparoscopic sleeve gastrectomy: Experience of Padua Bariatric Unit
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Belligoli, Anna, Zabeo, Eva, Serra, Roberto, Sanna, Marta, Conci, Scilla, Luca, Prevedello, Foletto, Mirto, Busetto, Luca, Frigo, ANNA CHIARA, Milan, Gabriella, and Vettor, Roberto
- Published
- 2014
22. Increased mitochondrial calcium uniporter in adipocytes underlies mitochondrial alterations associated with insulin resistance.
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Wright, Lauren E., Reane, Denis Vecellio, Milan, Gabriella, Terrin, Anna, Di Bello, Giorgia, Belligoli, Anna, Sanna, Marta, Foletto, Mirto, Favaretto, Francesca, Raffaello, Anna, Mammucari, Cristina, Nitti, Donato, Vettor, Roberto, and Rizzuto, Rosario
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FAT cells ,MITOCHONDRIA ,INSULIN resistance - Abstract
Intracellular calcium influences an array of pathways and affects cellular processes. With the rapidly progressing research investigating the molecular identity and the physiological roles of the mitochondrial calcium uniporter (MCU) complex, we now have the tools to understand the functions of mitochondrial Ca2 + in the regulation of pathophysiological processes. Herein, we describe the role of key MCU complex components in insulin resistance in mouse and human adipose tissue. Adipose tissue gene expression was analyzed from several models of obese and diabetic rodents and in 72 patients with obesity as well as in vitro insulin-resistant adipocytes. Genetic manipulation of MCU activity in 3T3-L1 adipocytes allowed the investigation of the role of mitochondrial calcium uptake. In insulin-resistant adipocytes, mitochondrial calcium uptake increased and several MCU components were upregulated. Similar results were observed in mouse and human visceral adipose tissue (VAT) during the progression of obesity and diabetes. Intriguingly, subcutaneous adipose tissue (SAT) was spared from overt MCU fluctuations. Furthermore, MCU expression returned to physiological levels in VAT of patients after weight loss by bariatric surgery. Genetic manipulation of mitochondrial calcium uptake in 3T3-L1 adipocytes demonstrated that changes in mitochondrial calcium concentration ([Ca
2+ ]mt) can affect mitochondrial metabolism, including oxidative enzyme activity, mitochondrial respiration, membrane potential, and reactive oxygen species formation. Finally, our data suggest a strong relationship between [Ca2+ ]mt and the release of IL-6 and TNFa in adipocytes. Altered mitochondrial calcium flux in fat cells may play a role in obesity and diabetes and may be associated with the differential metabolic profiles of VAT and SAT. [ABSTRACT FROM AUTHOR]- Published
- 2017
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23. Endogenous interferon-alpha level is increased in hepatitis C virus (HCV)-positive pregnant women
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Paternoster, D. M., Belligoli, A, Ngaradoumbe, N. K., Visentin, Silvia, Franco, R, Fagiuoli, S, Boldrin, C, Palu', Giorgio, Baldo, Vincenzo, Floreani, Annarosa, Belligoli, Anna, Paternoster, D, Belligoli, A, Ngaradoumbe, N, Visentin, S, Franco, R, Fagiuoli, S, Boldrin, C, Palù, G, Baldo, V, and Floreani, A
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Adult ,Hepacivirus ,Hepatitis C virus ,Alpha interferon ,HIV Infections ,medicine.disease_cause ,Pregnancy ,Risk Factors ,Medicine ,Humans ,Prospective Studies ,Pregnancy Complications, Infectious ,Interferon alfa ,biology ,business.industry ,Gastroenterology ,Infant, Newborn ,virus diseases ,HIV ,Interferon-alpha ,Hepatitis C ,Viral Load ,biology.organism_classification ,medicine.disease ,Delivery, Obstetric ,digestive system diseases ,Infectious Disease Transmission, Vertical ,Case-Control Studies ,Immunology ,HCV ,Coinfection ,Interferon ,Vertical transmission ,Female ,Viral disease ,business ,Viral load ,medicine.drug - Abstract
BACKGROUND: Mother-to-child transmission of hepatitis C virus (HCV) has been reported in around 5% of cases, and is much more likely to occur in case of coinfection with HIV. However, other cofactors influencing the vertical transmission are still debated. AIM: To assess the serum concentration of endogenous interferon (IFN) during pregnancy, and its eventual role on the vertical transmission of HCV. METHODS: Forty-seven HCV-infected pregnant women, and 3 control groups: (1) 75 HCV-negative pregnant women; (2) 29 HCV-positive nonpregnant women; (3) 29 HCV-negative nonpregnant women entered into the study. Endogenous IFN was assayed by enzyme-linked immunosorbent assay. The following parameters were also analyzed: viral load, HIV infection, risk factors for acquiring HCV, parity, gestational age, mode and course of delivery. RESULTS: Vertical transmission of HCV was observed in 2 cases (4.3%). Plasma levels of IFN were significantly higher in HCV-positive pregnant women compared with either HCV-positive and HCV-negative nonpregnant women. The 2 mothers who transmitted the infection had IFN levels within the same range as the women who did not transmit the infection. CONCLUSIONS: In HCV-positive pregnant women, there is an increased production of endogenous IFN-α. Further studies are warranted for clarifying the mechanisms of this cytokine in the prevention of HCV transmission. © 2008 Lippincott Williams & Wilkins, Inc.
- Published
- 2008
24. Risk Factors for Spontaneously Self-Reported Postprandial Hypoglycemia After Bariatric Surgery.
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Nannipieri, Monica, Belligoli, Anna, Guarino, Daniela, Busetto, Luca, Moriconi, Diego, Fabris, Roberto, Mari, Andrea, Baldi, Simona, Anselmino, Marco, Foletto, Mirto, Vettor, Roberto, and Ferrannini, Ele
- Published
- 2016
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25. Assessment of Protein Intake in the First Three Months after Sleeve Gastrectomy in Patients with Severe Obesity.
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Bertoni, Lucrezia, Valentini, Romina, Zattarin, Alessandra, Belligoli, Anna, Bettini, Silvia, Vettor, Roberto, Foletto, Mirto, Spinella, Paolo, Busetto, Luca, and Devlieger, Roland
- Abstract
An adequate protein intake prevents the loss of fat-free mass during weight loss. Laparoscopic sleeve gastrectomy (SG) jeopardizes protein intake due to post-operative dietary restriction and intolerance to protein-rich foods. The purpose of this study is to evaluate protein intake in the first three months after SG. We evaluated, 1 month and 3 months after surgery, 47 consecutive patients treated with SG. Protein intake, both from foods and from protein supplementation, was assessed through a weekly dietary record. Patients consumed 30.0 ± 10.2 g of protein/day on average from foods in the first month, with a significant increase to 34.9 ± 4.8 g of protein/day in the third month (p = 0.003). The use of protein supplementation significantly increased total protein intake to 42.3 ± 15.9 g protein/day (p < 0.001) in the first month and to 39.6 ± 14.2 g of protein/day (p = 0.002) in the third one. Compliance with supplement consumption was 63.8% in the first month and only 21.3% in the third month. In conclusion, both one and three months after SG, protein intake from foods was not sufficient. The use of modular supplements seems to have a significant impact on protein intake, but nevertheless it remains lower than recommended. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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26. White Adipose Tissue Expansion in Multiple Symmetric Lipomatosis Is Associated with Upregulation of CK2, AKT and ERK1/2.
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Sanna, Marta, Borgo, Christian, Compagnin, Chiara, Favaretto, Francesca, Vindigni, Vincenzo, Trento, Mariangela, Bettini, Silvia, Comin, Alessandra, Belligoli, Anna, Rugge, Massimo, Bassetto, Franco, Donella-Deana, Arianna, Vettor, Roberto, Busetto, Luca, and Milan, Gabriella
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WHITE adipose tissue ,LIPOMATOSIS ,TISSUE expansion ,FAT cells ,STEM cells ,ADIPOSE tissues - Abstract
Multiple symmetric lipomatosis (MSL) is a rare disorder characterized by overgrowing lipomatous tissue (LT) in the subcutaneous adipose tissue (SAT). What LT is and how it expands are not completely understood; previous data suggested that it could derive from brown AT precursors. In six MSL type I patients, we compared LT morphology by histological and immunohistochemistry (IHC) analysis, gene expression, by qPCR, kinase activity, by Western Blot and in vitro assay to paired-control SAT using AT from patients with pheochromocytoma as a human browning reference. In the stromal vascular fraction (SVF), we quantified adipose stem cells (ASCs) by flow cytometry, the proliferation rate, white and beige adipogenic potential and clonogenicity and adipogenicity by a limiting dilution assay. LT displayed white AT morphology and expression pattern and did not show increased levels of the brown-specific marker UCP1. In LT, we evidenced AKT, CK2 and ERK1/2 hyperactivation. LT-SVF contained increased ASCs, proliferated faster, sprouted clones and differentiated into adipocytes better than the control, displaying enhanced white adipogenic potential but not increased browning compared to SAT. In conclusion, LT is a white AT depot expanding by hyperplasia through increased stemness and enhanced white adipogenesis upregulating AKT, CK2 and ERK1/2, which could represent new targets to counteract MSL. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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27. CK2 modulates adipocyte insulin-signaling and is up-regulated in human obesity.
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Borgo C, Milan G, Favaretto F, Stasi F, Fabris R, Salizzato V, Cesaro L, Belligoli A, Sanna M, Foletto M, Prevedello L, Vindigni V, Bardini R, Donella-Deana A, and Vettor R
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- 3T3-L1 Cells, Adipose Tissue, White pathology, Animals, Biological Transport, Glucose metabolism, Humans, Liver metabolism, Mice, Muscle, Skeletal metabolism, Adipocytes pathology, Casein Kinase II metabolism, Insulin metabolism, Obesity metabolism, Obesity pathology, Signal Transduction, Up-Regulation
- Abstract
Insulin plays a major role in glucose metabolism and insulin-signaling defects are present in obesity and diabetes. CK2 is a pleiotropic protein kinase implicated in fundamental cellular pathways and abnormally elevated in tumors. Here we report that in human and murine adipocytes CK2-inhibition decreases the insulin-induced glucose-uptake by counteracting Akt-signaling and GLUT4-translocation to the plasma membrane. In mice CK2 acts on insulin-signaling in adipose tissue, liver and skeletal muscle and its acute inhibition impairs glucose tolerance. Notably, CK2 protein-level and activity are greatly up-regulated in white adipose tissue from ob/ob and db/db mice as well as from obese patients, regardless the severity of their insulin-resistance and the presence of pre-diabetes or overt type 2 diabetes. Weight loss obtained by both bariatric surgery or hypocaloric diet reverts CK2 hyper-activation to normal level. Our data suggest a central role of CK2 in insulin-sensitivity, glucose homeostasis and adipose tissue remodeling. CK2 up-regulation is identified as a hallmark of adipose tissue pathological expansion, suggesting a new potential therapeutic target for human obesity.
- Published
- 2017
- Full Text
- View/download PDF
28. Endogenous interferon-alpha level is increased in hepatitis C virus (HCV)-positive pregnant women.
- Author
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Paternoster DM, Belligoli A, Ngaradoumbe NK, Visentin S, Franco R, Fagiuoli S, Boldrin C, Palù G, Baldo V, and Floreani A
- Subjects
- Adult, Case-Control Studies, Delivery, Obstetric methods, Female, HIV Infections complications, Hepatitis C complications, Humans, Infant, Newborn, Pregnancy, Prospective Studies, Risk Factors, Viral Load, Hepatitis C blood, Hepatitis C transmission, Infectious Disease Transmission, Vertical, Interferon-alpha blood, Pregnancy Complications, Infectious blood, Pregnancy Complications, Infectious virology
- Abstract
Background: Mother-to-child transmission of hepatitis C virus (HCV) has been reported in around 5% of cases, and is much more likely to occur in case of coinfection with HIV. However, other cofactors influencing the vertical transmission are still debated., Aim: To assess the serum concentration of endogenous interferon (IFN) during pregnancy, and its eventual role on the vertical transmission of HCV., Methods: Forty-seven HCV-infected pregnant women, and 3 control groups: (1) 75 HCV-negative pregnant women; (2) 29 HCV-positive nonpregnant women; (3) 29 HCV-negative nonpregnant women entered into the study. Endogenous IFN was assayed by enzyme-linked immunosorbent assay. The following parameters were also analyzed: viral load, HIV infection, risk factors for acquiring HCV, parity, gestational age, mode and course of delivery., Results: Vertical transmission of HCV was observed in 2 cases (4.3%). Plasma levels of IFN were significantly higher in HCV-positive pregnant women compared with either HCV-positive and HCV-negative nonpregnant women. The 2 mothers who transmitted the infection had IFN levels within the same range as the women who did not transmit the infection., Conclusions: In HCV-positive pregnant women, there is an increased production of endogenous IFN-alpha. Further studies are warranted for clarifying the mechanisms of this cytokine in the prevention of HCV transmission.
- Published
- 2008
- Full Text
- View/download PDF
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