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Since the diffraction behavior of a light field is fully determined by its spatial wavefunction, i.e., its spatial complex amplitude (SCA), full characterization of spatial wavefunction plays a vital role in modern optics from both the fundamental and applied aspects. In this work, we present a novel “complex-amplitude profiler” based on spatial Stokes tomography with the capability to fully determine the SCA of a light field in a single shot with high precision and resolution. The SCA slice observed at any propagation plane provides complete information about the light field, thus allowing us to further retrieve the complete beam structure in the 3D space as well as the exact modal constitution in terms of spatial degrees of freedom. The principle demonstrated here provides an important advancement for the full characterization of light beams with a broad spectrum of potential applications in various areas of optics, especially for the growing field of structured light.