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Objective: To ascertain the prevalence and antibiotic susceptibility pattern of bacteria from urinary tract infections. Methods: A retrospective analysis of bacterial pathogens and antibiogram was done in Helping Hands Community Hospital Laboratory from August to November, 2014. Results: The prevalence of bacterial isolates was higher among females (44.70%) than males (31.34%) (P < 0.001). The most common bacteria were Escherichia coli 35 (59.32%), followed by coagulase-negative Staphylococcus 5 (8.47%), Salmonella enterica 4 (6.78%), Proteus mirabilis 2 (3.39%), Proteus vulgaris 2 (3.39%), Klebsiella pneumoniae 2 (3.39%), Salmonella typhi 2 (3.39%), Pseudomonas species 2 (3.39%), Staphylococcus aureus 2 (3.39%), Citrobacter species 2 (3.39%), Acinetobacter baumannii 1 (1.69%), Acinetobacter species 1 (1.69%), Proteus vulgaris 1 (1.69%). Conclusions: Most Gram-positive isolates were sensitive to nitrofurantoin, gentamycin and norfloxacin, and they are considered as appropriate microbials for empirical treatment of urinary tract infections.