6 results on '"Bolanos-Carriel, Carlos"'
Search Results
2. Tri5 gene expression analysis during postharvest storage of wheat grain from field plots treated with a triazole and a strobilurin fungicide
- Author
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Bolanos-Carriel, Carlos, Wegulo, Stephen N., Baenziger, P. Stephen, Eskridge, Kent M., Funnell-Harris, Deanna, McMaster, Nicole J., Schmale, David G. III, Hallen-Adams, Heather E., and School of Plant and Environmental Sciences
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triazole ,Fusarium head blight ,wheat ,deoxynivalenol ,gene expression ,food and beverages ,trichodiene synthase ,strobilurin - Abstract
Fusarium head blight (FHB) and the associated mycotoxin deoxynivalenol (DON) negatively impact the wheat industry worldwide. In North America, FHB is mainly caused by Fusarium graminearum sensu stricto. The purpose of this study was to evaluate, under storage conditions, the expression of the DON biosynthetic gene trichodiene synthase (Tri5) of F. graminearum in grain of hard red winter wheat cultivars 'Overley' (FHB-susceptible) and 'Overland' (moderately resistant to FHB) from field plots treated or untreated with the triazole fungicide Prosaro and the strobilurin fungicide Headline. Infected grain was stored and periodically sampled to determine gene expression by qRT-PCR analysis. The F. graminearum housekeeping gene GAPDH was consistently detected, indicative of metabolically active fungi, and Tri5 detection was significantly higher in 'Overley' compared with 'Overland'. Tri5 gene expression and DON concentrations showed little to no correlation; consequently, Tri5 expression levels did not accurately predict DON concentrations. The strobilurin did not significantly reduce Tri5 gene expression compared with untreated wheat. In the triazole treatment, a significant reduction in the relative expression of Tri5 was detected after 120 days, as well as a downregulation of Tri5 from 60 to 120 days of storage in 'Overley'. In grain from strobilurin-treated plots of both cultivars, the expression of Tri5 increased from 0 to 30 days after tempering. Genetic expression of Tri5 that is necessary for the production of DON can increase during storage of high-moisture grain. Fusarium fungi can persist in wheat kernels for several months postharvest and may actively produce toxin during this period. U.S. Department of AgricultureUnited States Department of Agriculture (USDA); Secretariat of Higher Education, Science, Technology and Innovation of Ecuador; Nebraska Wheat Board; U.S. Wheat and Barley Scab InitiativeUnited States Department of Agriculture (USDA) [59-0790-7-080, 422288, 422533]; Hatch Multistate Research Capacity Funding Program [1006715] This work was supported by the U.S. Department of Agriculture; The Secretariat of Higher Education, Science, Technology and Innovation of Ecuador; Nebraska Wheat Board; U.S. Wheat and Barley Scab Initiative [59-0790-7-080; #422288; #422533]; Hatch Multistate Research Capacity Funding Program [1006715]. Public domain – authored by a U.S. government employee
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- 2020
3. Effects of field-applied fungicides, grain moisture, and time on deoxynivalenol during postharvest storage of winter wheat grain.
- Author
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Bolanos-Carriel, Carlos, Wegulo, Stephen N., Hallen-Adams, Heather, Baenziger, P. Stephen, Eskridge, Kent M., Funnell-Harris, Deanna, McMaster, Niki, and Schmale III, David G.
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WINTER grain ,GRAIN ,MOISTURE ,WINTER wheat ,HUMIDITY ,FUNGICIDES ,POSTHARVEST losses of crops ,GRAIN storage - Abstract
Copyright of Canadian Journal of Plant Science is the property of Canadian Science Publishing and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2020
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4. Bacterial canker caused by Clavibacter michiganensis subsp. michiganensis in naranjilla in Ecuador.
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Bolanos-Carriel, Carlos, Gallegos, Patricio, Ochoa, José B., Insuasti, María, Bonilla, Verónica, Evans, Mary H., Alvarez, Jorge, Ramirez, Dario, and León-Reyes, Antonio
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NARANJILLA , *CANKER (Plant disease) , *CLAVIBACTER michiganensis , *MICROBACTERIACEAE , *SOLANUM - Abstract
Bacterial canker of naranjilla or lulo (Solanum quitoense and Solanum pectinatum) is a new disease that could completely destroy naranjilla plants. The objectives of this study were to identify and characterize bacteria associated with canker wilt of naranjilla, and to determine the importance of wounds in the dissemination and transmission of bacterial canker. Symptoms of this disorder consist of dieback and leaves showing incurvature which was diagnostic to confirm bacterial wilt. Collapse of leaf panel on Nicotiana tabacum confirmed that the bacterium is phytopathogenic. This bacterium showed positive serological tests for Clavibacter michiganensis subsp. michiganensis, positive Gram reaction, growth on NCP 88, (ELISA+ GRAM+ NCP 88 +) as well as re-isolation through Koch postulates in Solanum cheesmanii. Aerial wounds appear to be the most important means for dissemination. More research is needed about the molecular characterization of the bacterium, and potential for the bacterium to be transmitted to other solanaceous crops. [ABSTRACT FROM AUTHOR]
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- 2017
5. First Report of Fusarium boothii Causing Head Blight of Wheat in the United States
- Author
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Wegulo, Stephen N., Valverde-Bogantes, Esteban, Bolanos-Carriel, Carlos, Hallen-Adams, Heather, Huebner, Andreia Bianchini, McMaster, Niki, and Schmale, David Garner
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Epidemics ,Wheat ,Business, international - Abstract
In NorthAmerica, Fusarium graminearum is the main causal agent of Fusarium head blight (FHB), an important wheat disease in the region. In 2015, severe and widespread FHB epidemics occurred in wheat in Nebraska, USA. Symptomatic wheat spike samples (20 to 30 spikes/sample) at the hard dough growth stage were collected from 23 fields (1 sample/field) in 15 counties representing all the major wheat growing regions in the state (southeast, south central, southwest, and northwest). DNA was extracted from single-spored isolates grown on PDA using the DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). A portion of the translation elongation factor 1-[alpha] (EF-1[alpha]) gene from a subset of the isolates was amplified using primers EF-1f (CGACCACTGTGAGTACCA) and EF-1r (GTCAAGAACCCAGGCGTA) (Ward et al. 2008) and bidirectionally sequenced. The sequences, deposited in GenBank under accession numbers MH379136 to MH379138 and MH392474 to MH392477, were analyzed and assembled in MEGA7, and BLAST queries were performed in FusariumID (http://isolate.fusariumdb.org/guide.php) and Fusarium MLST (http://www.westerdijkinstitute.nl/fusarium/). Three isolates from Chase County in the southwest (isolates NE16-15Fb and NE19-15Fb) and Box Butte County (isolate NE21-15Fb) in the northwest had 100% similarity with the reference strain F. boothii NRRL 29105. The remainder were identified as F. graminearum based on similarity to F. graminearum NRRL 28336 ranging from 99.51% to 100%. To fulfill Koch's postulates, FHB-susceptible spring wheat cv. Wheaton was grown in the greenhouse in 15-cm-diameter pots, 12 plants per pot (experimental unit). Spikes were spray-inoculated at anthesis with spores of F. boothii isolates NE16-15Fb and NE19-15Fb and a single isolate of F. graminearum (NE20-15Fg) collected in the same survey in Kimball County in the southwest (ca. 1 X 105 spores/mL) or not inoculated, and enclosed in transparent Ziploc bags for 72 h. A randomized complete block design with five or six replications was used in two experiments. Symptoms (bleaching of spikeiets) appeared 3 days after inoculation (dai) in all three isolate treatments. At 21 dai, FHB severity (percent of bleached spikeiets on a spike) ranged from 18% to 87%. No symptoms developed in the check treatment. Mature grain was ground to flour and tested for deoxynivalenol (DON) and its acetylated derivatives 3-ADON and 15-ADON using gas chromatography/mass spectrometry (GC/MS). All three isolates had the 15-ADON chemotype. Single-spored cultures of the three isolates were recovered on PDA from symptomatic kernels obtained from both experiments. The three isolates produced fertile perithecia on carrot agar after incubation at room temperature for 7 weeks. Their colonies on PDA resembled typical F. graminearum colonies (Leslie and Summerell 2006). Measurements of ascospores (17.50-32.50 [micro]m X 3.75-7.50 urn) and macroconidia (36.25-67.50 [micro]m X 3.75-6.25 [micro]m) of the three isolates were similar to those reported for species in the F. graminearum clade (O'Donnell et al. 2004). F. boothii has been reported as the cause of FHB of wheat or Gibberella ear rot of maize in several countries including Mexico (Ceron-Bustamante et al. 2018) and South Africa (Boutigny et al. 2011). To our knowledge, this is the first report of F. boothii causing FHB of wheat in the United States. Research will be needed to determine its distribution, aggressiveness, and impact on yield in single or double infections with F. graminearum., https://apsjournals.apsnet.Org/doi/abs/10.1094/PDIS-04-18-0696-PDN Dr. Stephen N. Wegulo, Mr. Esteban Valverde-Bogantes, Mr. Carlos Bolanos-Carriel, Dr. Heather Hallen-Adams, Dr. Andreia Bianchini Huebner, Ms. Niki McMaster, Dr. David Garner Schmale [...]
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- 2018
6. Auxin Profiling and GmPIN Expression in Phytophthora so/ae-Soybean Root Interactions.
- Author
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Stasko, Anna K., Batnini, Amine, Bolanos-Carriel, Carlos, Jinshan Ella Lin, Yun Lin, Blakeslee, Joshua J., and Dorrance, Anne E.
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JASMONATE , *PLANT hormones , *PHYTOPHTHORA sojae , *PHYTOPHTHORA , *AUXIN , *PLANT-microbe relationships , *DISEASE resistance of plants - Abstract
Auxin (indole-3-acetic acid, IAA) has been implicated as a susceptibility factor in both beneficial and pathogenic molecular plant-microbe interactions. Previous studies have identified a large number of auxin-related genes underlying quantitative disease resistance loci (QDRLs) for Phytophthora sojae. Thus, we hypothesized that auxin may be involved the P. sojae-soybean interaction. The levels of IAA and related metabolites were measured in mycelia and media supernatant as well as in mock and inoculated soybean roots in a time course assay. The expression of 11 soybean Pin-formed (GmPIN) auxin efflux transporter genes was also examined. Tryptophan, an auxin precursor, was detected in the P. sojae mycelia and media supernatant. During colonization of roots, levels of IAA and related metabolites were significantly higher in both moderately resistant Conrad and moderately susceptible Sloan inoculated roots compared with mock controls at 48 h postinoculation (hpi) in one experiment and at 72 hpi in a second, with Sloan accumulating higher levels of the auxin catabolite IAA-Ala than Conrad. Additionally, one GmPIN at 24 hpi, one at 48 hpi, and three at 72 hpi had higher expression in inoculated compared with the mock control roots in Conrad. The ability of resistant cultivars to cope with auxin accumulation may play an important role in quantitative disease resistance. Levels of jasmonic acid (JA), another plant hormone associated with defense responses, were also higher in inoculated roots at these same time points, suggesting that JA also plays a role during the later stages of infection. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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