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In this study, the effectiveness of using cluster analysis in determining the changes in the number of animals in the field of goat and buffalo breeding in the TRB1 region was tested. The numbers of goats and buffalos used in the research were obtained from the records kept by TÜİK between 2004 and 2023. According to the cluster analysis results, the numbers of buffalos in Elazığ and Bingöl provinces was found to be higher than Malatya and Tunceli provinces between 2004 and 2023. According to the specified years, while Bingöl province was found to be higher than other provinces in terms of the numbers of goats, it was determined that Malatya province had the lowest values. When analyzed by year, the numbers of buffalos in the provinces of the TRB1 region for the years 2004-2006 was found to be higher than the following years. In other words, it is understood that the numbers of buffalos has been decreasing in the TRB1 region since 2007. In the TRB1 region, goat numbers between 2012- 2023 were found to be higher than those between 2004-2011. Accordingly, it can be said that there has been an increase in the number of goats in the TRB1 region since 2012. In the TRB1 region, appropriate regulations need to be made, taking into account the differences between provinces and years in terms of the numbers of buffalos and goats. In this study, it was seen that cluster analysis could be used successfully in determining the differences in animal numbers according to provinces and years. [ABSTRACT FROM AUTHOR]

The correlation between peripheral and seminal hormones and biochemical parameters with sperm kinematics across seasons in buffalo bulls were studied. Semen from buffalo bulls (n = 13) during different seasons viz. summer, comfort (spring and autumn) and winter was collected. Sperm kinematics of ejaculates (n = 39) (three ejaculates per bull) were carried out during each season and simultaneously blood (10 mL) was collected and serum was separated for hormones (leptin, testosterone and kisspeptin) and biochemical [heat shock protein 70 (HSP70), ascorbic acid and total antioxidant capacity] estimation. Total motility (TM, %), progressive motility (PM, %) and rapid motility (RM, %) showed significant differences across seasons with the highest TM, PM and RM during winter. HSP70 was higher in seminal plasma levels during summer and TAC was higher in serum than seminal plasma during all seasons. In conclusion, seminal leptin and ascorbic acid showed an association with sperm kinematics across seasons in buffalo bulls. [ABSTRACT FROM AUTHOR]

Mastitis is a common economically important disease of dairy buffaloes. Antibiotic resistance is the most cited reason behind the failure of clinical mastitis treatment in buffaloes. Selection of antibiotic after antibiotic sensitivity test is the key to avoid multidrug resistance (MDR) for mastitis therapy. Therefore, the present research work was carried out to evaluate efficacy of moxifloxacin and marbofloxacin in clinical mastitis in buffaloes based on the sensitivity against common mastitic bacteria in buffaloes. Total twenty clinical mastitis positive buffaloes were selected after screening of 129 lactating buffaloes by strip cup test and clinical signs, in and around Parbhani area. The selected buffaloes were divided into 2 groups, 10 animals in each. Group I was treated with injection Moxifloxacin @ 5 mg/kg BW IM for 5 days and group II received injection Marbofloxacin @ 2 mg/kg BW IM for 5 days. Supportive treatment was given in both groups as antihistaminic, multivitamins and NSAIDs. The clinical parameters like rectal temperature, heart rate and respiratory rate showed significant improvement in both the treatment groups. Haematolgical study showed significant improvement in TLC, neutrophil and lymphocyte count while apparent but non-significant improvement observed in Hb, PCV, basophil, eosinophil and monocyte count. The most frequent bacteria observed in mastitic samples were Staphylococcus aureus, followed by E. coli, Klesiella spp. and mixed bacterial infection. Marbofloxacin showed highest sensitivity to mastitis causing bacteria followed by Moxifloxacin, Enrofloxacin and Ciprofloxacin. The comparative efficacies in treatment groups were calculated on the basis of improvement in clinical signs, strip cup test and somatic cell count after treatment. Higher efficacy of Marbofloxacin was found (70%) as compared to Moxifloxacin (50%) for treatment of clinical mastitis. Hence, concluded that marbofloxacin could be appropriative antibiotic agent in the treatment of mastitis in buffalo. [ABSTRACT FROM AUTHOR]

Introduction: Immunoglobulin (Ig) refers to the nomenclature for all antibody proteins produced by B lymphocytes. The genetic locus encoding Ig is critical for vertebrate humoral immune responses and diverse antibody repertoires. Despite the critical role of buffaloes as livestock and their significance in disease transmission, the Ig loci of this species have not been thoroughly annotated. This study aimed to systematically characterize the Ig loci in buffaloes and their unique features, providing a foundation for understanding buffalo immune function. Methods: The genomic assembly of Murrah buffalo (NDDB_SH_1) was analyzed to annotate Ig loci. Annotation criteria included functional motifs, RSS sequences, and structural features of V, D, J, and C genes. The CDR3 repertoires were constructed using genomic DNA extracted from spleen samples of five healthy buffaloes. High-throughput sequencing of multiplex PCR products enabled repertoire analysis, and MiXCR software was used for alignment and CDR3 extraction. Repertoire diversity, gene usage, and clonal frequencies were analyzed using the Immunarch R package. Results: The IgH locus spans approximately 667 kb on chromosome 20, containing two D-J-C clusters, 54 VH genes, 10 DH genes, 8 JH genes, and 9 CH genes. The Igκ locus, located on chromosome 12, encompasses 24 Vκ genes, 5 Jκ genes, and 1 Cκ gene, while the Igλ locus on chromosome 17 includes 71 Vλ genes, 3 Jλ genes, and 3 Cλ genes. We also conducted a detailed examination of the buffalo IgH CDR3 repertoire, revealing the presence of ultra-long CDR3 sequences, a biased usage of certain V genes, and a high-frequency usage of IgHJ1-4 genes. Furthermore, we identified a set of shared clonotypes across the samples, highlighting commonalities in the buffalo antibody repertoire. Conclusion: These findings contribute to the understanding of buffalo immune function and provide insights into the evolution and diversity of ruminant immunoglobulin genes. [ABSTRACT FROM AUTHOR]

Background: Water buffaloes represent a crucial genetic resource for the global dairy industry, yet enhancements in their production performance remain relatively constrained. The advent of advanced sequencing technologies, coupled with genome-wide association studies (GWASs), has significantly boosted the potential for breeding superior-quality water buffalo. Methods: An integrated genomic analysis was performed on sequencing data from 100 water buffaloes, utilizing the high-quality UOA_WB_1 genome assembly as a reference. This study particularly emphasized reproduction-related traits, with a focus on age at first calving (AFC). Results: Our analysis revealed two significant single-nucleotide polymorphisms (SNPs). Based on these genetic markers, the GRM1 gene was identified as a candidate gene. This gene shows substantial involvement in various reproduction-associated pathways, including the FOXO signaling pathway, calcium signaling pathway, and estrogen signaling pathway. Conclusions: The identification of GRM1 as a candidate gene provides a robust theoretical basis for molecular breeding strategies aimed at enhancing fertility in water buffaloes. These findings offer critical scientific support for optimizing breeding programs, thereby improving overall production efficiency. [ABSTRACT FROM AUTHOR]

Background: Buffaloes are considered an indispensable genetic resource for dairy production. However, improvements in lactation performance have been relatively limited. Advances in sequencing technology, combined with genome-wide association studies, have facilitated the breeding of high-quality buffalo. Methods: We conducted an integrated analysis of genomic sequencing data from 120 water buffalo, the high-quality water buffalo genome assembly designated as UOA_WB_1, and milk production traits, including 305-day milk yield (MY), peak milk yield (PM), total protein yield (PY), protein percentage (PP), fat percentage (FP), and total milk fat yield (FY). Results: The results identified 56 significant SNPs, and based on these markers, 54 candidate genes were selected. These candidate genes were significantly enriched in lactation-related pathways, such as the cAMP signaling pathway (ABCC4), TGF-β signaling pathway (LEFTY2), Wnt signaling pathway (CAMK2D), and metabolic pathways (DGAT1). Conclusions: These candidate genes (e.g., ABCC4, LEFTY2, CAMK2D, DGAT1) provide a substantial theoretical foundation for molecular breeding to enhance milk production in buffaloes. [ABSTRACT FROM AUTHOR]

A detailed understanding of the precise regulatory mechanisms governing buffalo skeletal muscle is crucial for improving meat quality and yield. Proper skeletal muscle fate decisions necessitate the accurate regulation of key enhancers. This study screened nine potential enhancers linked to muscle development by analysing ATAC-seq data from buffalo myoblasts during the proliferative and differentiative phases. The enhancer activity of these candidates was confirmed in buffalo myoblasts, C2C12, and human skeletal muscle myoblasts using a dual-luciferase reporter system. The CRISPRi system and RT-qPCR were used to test the effects of 9 candidate enhancers on buffalo myoblasts. The active enhancer, Enh483, was selected based on its significant impact. Upon successful inhibition of Enh483 using CRISPRi, decreases in the expression of buffalo myogenic proliferation marker genes (PCNA, CyclinD1, and CDK2) were observed via RT-qPCR and Western blot. Subsequent proliferation assays using CCK-8 and EdU confirmed the promotive effect of Enh483 on buffalo myogenic cell proliferation. Following a 5-day differentiation induction period, changes in the expression of differentiation marker genes (MyoD1, MyoG, and MyHC) were analysed using RT-qPCR and Western blot. Additionally, fused myotube numbers were quantified, and the impact of Enh483 on buffalo myogenic cell differentiation was assessed through immunofluorescence. Our findings indicate that Enh483 facilitates buffalo myogenic cell differentiation. Further interaction analysis utilising 3C-PCR revealed a direct association between Enh483 and the FAXC promoter. In summary, the results from this study lay a foundational framework for deciphering the intricate regulatory mechanisms underpinning buffalo muscle development. [ABSTRACT FROM AUTHOR]

Context: The limited availability of recipient oocytes poses significant challenges in increasing the population of endangered species. Aims: This study demonstrates the use of oocytes from closely related domestic species (cattle, buffalo, and goat) in the production of gaur embryos via interspecies somatic cell nuclear transfer (iSCNT). The developmental abilities of embryos from various combinations – gaur–bovine, gaur–buffalo, gaur–goat, and others – are analyzed. Previous achievements are retrospectively reviewed and summarized. Methods: The methodologies for iSCNT and in vitro gaur embryo production are outlined. Key results: Blastocyst rates among gaur–bovine, gaur–buffalo, and gaur–goat embryos showed no significant differences (P < 0.05). Gaur–bovine embryo development was comparable to intraspecies embryos (bovine–bovine), indicating successful developmental compatibility. Pregnancy rates after transferring gaur–bovine embryos were reported for the first time, with rates of 4.76%, 42.86%, 19.05% and 4.76% at Days 45, 60, 90 and 120, respectively. Conclusions: Bovine, buffalo, and goat oocyte cytoplasm is effective for producing gaur iSCNT embryos, capable of initiating early pregnancies. Implications: Laboratory techniques and methodologies can save costs and time, contributing to the conservation of endangered species. Bovine, buffalo, and goat oocyte cytoplasm is effective for producing gaur iSCNT embryos, capable of initiating early pregnancies. iSCNT is probably one of the promising reproductive technologies for increasing the gaur. The use of taxonomy closely related animal species applied to methodology and techniques by optimizing culture conditions and procedures across closely related species would greatly benefit laboratory production and field implication (enhancing the chance of probability of surrogate mother), and also saving cost and time, to increase the population size of endangered species. [ABSTRACT FROM AUTHOR]

Background: African buffalo (Syncerus caffer) is a significant reservoir host for many zoonotic and parasitic infections in Africa. These include a range of viruses and pathogenic bacteria, such as tick-borne rickettsial organisms. Despite the considerations of mammalian blood as a sterile environment, blood microbiome sequencing could become crucial for agnostic biosurveillance. This study investigated the blood microbiome of clinically healthy wild buffaloes in Kenya to determine its applicability in agnostic testing for bacteria in apparently healthy wild animals. Methods: Whole blood and serum samples were collected from 46 wild African buffalos from Meru National Park (30), Buffalo Springs (6) and Shaba (10) National Reserves in upper eastern Kenya. Total deoxyribonucleic acid (DNA) was extracted from these samples and subjected to amplicon-based sequencing targeting the 16 S rRNA gene. The bacteria operational taxonomic units (OTU) were identified to species levels by mapping the generated V12 and V45 regions of 16 S rRNA gene to the SILVA database. These OTU tables were used to infer the microbial abundance in each sample type and at the individual animal level. The sequences for the corresponding OTUs were also used to generate phylogenetic trees and thus infer evolution for the OTUs of interest. Results: Here, we demonstrate that buffaloes harbor many bacteria in their blood. We also report a diversity of 16 S rRNA gene sequences for Anaplasma and Mycoplasma from individual animals. By sequencing both whole blood and serum in triplicate for each animal, we provide evidence of the differences in detecting bacteria in both sample types. Conclusions: Diverse bacteria, including some potential pathogens, can be found in the blood of clinically healthy wild African buffalo. Agnostic surveillance for such pathogens can be achieved through blood microbiome sequencing. However, considerations for the question being asked for the blood microbiome in wildlife will impact the choice for using whole blood or serum for sequencing. [ABSTRACT FROM AUTHOR]

This experiment was carried out in 2023 season of spring and fall growth cycle in a private canopy in Al-Radwaniyah city, Baghdad Governorate, study included addition of bacterial biofertilizer at four treatments: no addition (A1), addition of Stenotrophomonas maltophilia bacteria (A2), addition of Azospirillum brasilense bacteria (A3) and addition of both types of bacteria (A4) and organic fertilizer (Decomposing buffalo manure) at three levels (0, 250 and 500 g.soil -1 ), which are symbolized by B0, B1, and B2, respectively Added once during the spring and fall growing cycle, and spray moringa extract at three levels (0, 20 and 40 g.L -1 ), which are symbolized M0, M1, and M2, respectively spray 5 times every 15 days. Treatments were replicated three times at factorial experiment in a RCBD and thus number of transplants used was 108. Experimental results showed that adding two types of bacteria (A4) and buffalo manure at a level of 500 g.soil -1 (B2) and spraying moringa extract at a concentration of 40 g.L -1 (M2) as interaction treatment A4B2M2 produced best results in shoot number, stem diameter, leaf potassium and iron percent %, and epidermal and spongy cells layers thickness, (25.00, 41.00) shoot. plant -1, (14.247, 5.283) mm, (2.980, 2.885) %, (215.8, 220.7) mg.kg -1 for two growing seasons, respectively, and (48.60, 108.4) micrometer, respectively compared to control treatment. [ABSTRACT FROM AUTHOR]

The specific expression profile and function of circular RNA (circRNA) in follicular atresia remain largely unknown. Here, the circRNA expression profiles of granulosa cells derived from healthy follicles (HFs) and antral follicles (AFs) in buffalo were analyzed by RNA-seq, and the mechanism of a differentially expressed circRNA (DEcircRNA) circTEC regulating the granulosa cell function that affects follicular atresia was further explored. RNA-seq results showed that a total of 112 DEcircRNAs were identified. Among them, circTEC was highly expressed in HF, and its circular structure was confirmed by RNase R digestion assay, reversed PCR and Sanger sequencing. Functional experiments demonstrated that circTEC promotes the proliferation and steroid hormone synthesis of buffalo granulosa cells (bGCs), and it also inhibits their apoptosis. In-depth mechanism analysis showed that the expression level of circTEC in bGCs from AFs was adversely related to miR-144-5p and consistent with FZD3. CircTEC acts as an endogenous sponge of miR-144-5p to regulate the expression of the target gene FZD3 in AFs, which promotes the proliferation of bGCs and inhibits bGCs apoptosis, thereby inhibiting follicular atresia in buffalo. In summary, our study revealed the regulatory role of the circTEC/miR-144-5p/FZD3 axis during follicular atresia in buffalo. These results provided new insights into the biological mechanism underlying follicular atresia. [ABSTRACT FROM AUTHOR]

Abstract Background African buffalo (Syncerus caffer) is a significant reservoir host for many zoonotic and parasitic infections in Africa. These include a range of viruses and pathogenic bacteria, such as tick-borne rickettsial organisms. Despite the considerations of mammalian blood as a sterile environment, blood microbiome sequencing could become crucial for agnostic biosurveillance. This study investigated the blood microbiome of clinically healthy wild buffaloes in Kenya to determine its applicability in agnostic testing for bacteria in apparently healthy wild animals. Methods Whole blood and serum samples were collected from 46 wild African buffalos from Meru National Park (30), Buffalo Springs (6) and Shaba (10) National Reserves in upper eastern Kenya. Total deoxyribonucleic acid (DNA) was extracted from these samples and subjected to amplicon-based sequencing targeting the 16 S rRNA gene. The bacteria operational taxonomic units (OTU) were identified to species levels by mapping the generated V12 and V45 regions of 16 S rRNA gene to the SILVA database. These OTU tables were used to infer the microbial abundance in each sample type and at the individual animal level. The sequences for the corresponding OTUs were also used to generate phylogenetic trees and thus infer evolution for the OTUs of interest. Results Here, we demonstrate that buffaloes harbor many bacteria in their blood. We also report a diversity of 16 S rRNA gene sequences for Anaplasma and Mycoplasma from individual animals. By sequencing both whole blood and serum in triplicate for each animal, we provide evidence of the differences in detecting bacteria in both sample types. Conclusions Diverse bacteria, including some potential pathogens, can be found in the blood of clinically healthy wild African buffalo. Agnostic surveillance for such pathogens can be achieved through blood microbiome sequencing. However, considerations for the question being asked for the blood microbiome in wildlife will impact the choice for using whole blood or serum for sequencing.

Buffalo is a rustic animal; it can adapt and survive in various environments with different topography, climate and vegetation, and has a greater longevity than cattle. The high resistance to parasites, as well as the ability to improve the efficiency of feed consumption in case of forage shortage, means that even in marshy territories, where any other type of livestock or agricultural activity is impossible, buffalo farming can develop an economy. This review aims to present the key research topics, the evolution and geographic distribution of literature on buffalo welfare, and to underscore potentiality and existing knowledge gaps. A comprehensive search on the Scopus bibliometric database was carried out using various welfare-related keywords such as: ‘buffalo welfare’, ‘buffalo calf welfare’ and ‘buffalo heifers’ welfare’. The research identified 308 papers published in English from January 1992 to December 2023 that, after filtering, became 148 eligible records. Descriptive statistics revealed a significant exponential increase in studies on buffalo welfare since 2020, particularly in Italy, followed by Brazil. Text mining and topic analysis revealed that the most frequently mentioned terms were ‘milk’, ‘water’, ‘behaviour’, ‘farm’ and ‘stun’, confirming the role and the importance of this species in dairy production. Nine identified topics span diverse subject areas such as ‘slaughter process’, ‘dairy production’, ‘general welfare’, ‘housing system’, ‘heat stress’, ‘reproduction performance’, ‘health status’ ‘breeding management’, and ‘stress evaluation’. Although academia shows an increasing interest in buffalo behaviour and welfare, continued research is necessary to fully comprehend how to ensure the welfare of this important species.

Rectal thermometers are commonly used to assess animal health, but non-invasive methods like thermal imaging are gaining popularity for their ease. This study explores using infra-red thermography to measure eye temperatures as a proxy for body temperature in buffaloes. Among 280 adult female buffaloes, rectal temperature averaged 38.26 ± 0.38 °C, while eye temperature averaged 36.99 ± 0.47 °C. Statistical analysis showed a significant positive correlation (0.674, p

Abstract Background Indigenous buffaloes, as the important livestock species contributing to economy of the country, are the lifeline of livelihood in Nepal. They are distributed across diverse geographical regions of the country and have adapted to various feeding, breeding, and management conditions. The larger group of these native buffalo breeds are present in narrow and stiff hilly terrains. Their dispersal indicates a possible environmental adaptation mechanism, which is crucial for the conservation of these breeds. Results We utilized whole-genome sequencing (WGS) to investigate the genetic diversity, population structure, and selection signatures of Nepalese indigenous buffaloes. We compared 66 whole-genome sequences with 118 publicly available sequences from six river and five swamp buffalo breeds. Genomic diversity parameters indicated genetic variability level in the Nepalese buffaloes comparable to those of Indian breeds, and population genetic structure revealed distinct geography-mediated genetic differentiation among these breeds. We used locus-specific branch length analysis (LSBL) for genome-wide scan, which revealed a list of potentially selected genes in Lime and Parkote breeds that inhabit the hilly region. A gene ontology (GO) analysis discovered that many GO terms were associated with cardiac function regulation. Furthermore, complementary analyses of local selection signatures, tissue expression profiles, and haplotype differences identified candidate genes, including KCNE1, CSF1R, and PDGFRB, related to the regulation of cardiac and pulmonary functions. Conclusions This study is a comprehensive WGS-based genetic analysis of the native Nepalese buffalo breeds. Our study suggested that the Nepalese “hilly” buffaloes, especially the Lime and Parkote breeds, have undergone some characteristic genetic changes and evolved increased cardiac and pulmonary function for their adaptation to the steep hilly terrains of the country.

Background and Aim: Buffalo is the principal dairy animal and plays a major role in the economic growth of the dairy industry, contributing nearly 50% of the country’s milk production. The Buffalo core body temperature is typically 38.5°C, but it can rise to 41.5°C in the summer, causing heat stress, which leads to the generation of reactive oxygen species or oxidative stress and affects the reproductive physiology of animals. Curcumin acts as an antioxidant, improves cellular development, and combats the effect of heat stress on in vitro-produced embryos. This study aimed to examine the impact of curcumin on developmental competence and the expression of important genes under normal and heat-stressed conditions during in vitro embryo production in buffalo. Materials and Methods: Group-1: All embryo production steps (i.e., in vitro maturation [IVM], in vitro fertilization [IVF], and in vitro culture [IVC]) were conducted at 38.5°C. The presumed zygotes were cultured in media supplemented with different concentrations of curcumin, that is, 0 μM, 5 μM, and 10 μM of curcumin. Group-2: All embryo production steps (i.e., IVM, IVF, and IVC) were carried out at 38.5°C. The presumed zygotes were cultured in media supplemented with different concentrations of curcumin, that is, 0 μM, 5 μM, and 10 μM of curcumin, but the early cleaved embryos were exposed to heat stress (39.5°C) for 2 h after 48 h of IVF and then cultured at 38.5°C for embryo production. Results: Blastocyst production was 16.63 ± 1.49%, 21.46 ± 0.67%, and 6.50 ± 1.17% at control, 5 μM and 10 μM of curcumin at 38.5°C, respectively, whereas at 39.5°C, it was 8.59 ± 1.20%, 15.21 ± 1.31%, and 3.03 ± 1.20% at control, 5 μM and 10 μM curcumin, respectively. The blastocyst rate was found to be significantly higher (p < 0.05) at 5 μM curcumin compared with the control or 10 μM at 38.5°C and 39.5°C. The antioxidant, antiapoptotic, and pluripotency-related genes exhibited higher (p < 0.05) expression in the presence of 5 μM curcumin compared to 10 μM or control at both temperatures. Conclusion: Curcumin supplementation in embryo culture media effectively enhances embryo production in vitro and mitigates the adverse effects of heat stress.

Abstract Background Buffalo is a globally important livestock species, but its reproductive performance is relatively low than cattles. At present, dominant follicle development specific process and mechanistic role of follicular growth related genes in water buffaloes are not well understood. Therefore, we comprehensively performed transcriptomics of granulosa cells and oocytes from different-sized follicles in water buffalo to identify key candidate genes that influence follicle development and diameter, and further explored the potential regulatory mechanisms of granulosa cells and oocytes in the process of water buffalo follicle development. Results In this study, we found918 granulosa cell transcripts and 1401 oocyte transcripts were correlated in follicles of different diameters, and the expression differences were significant. Subsequent enrichment analysis of the co-expressed differentially expressed transcripts identified several genes targeted by long non-coding RNAs (lncRNAs) and associated with follicular development. Notably, the upregulation of BUB1 regulated by MSTRG.41325.4 and interactive action of SMAD2 and SMAD7 might have key regulatory role in follicular development. Additionally, we also detected key differentially expressed genes that potentially influence follicular hormone metabolism and growth, like ID2, CHRD, TGIF2 and MAD2L1, and constructed an interaction network between lncRNA transcripts and mRNAs. Conclusions In summary, this study preliminarily revealed the differences in gene expression patterns among buffalo follicles of different sizes and their potential molecular regulatory mechanisms. It provides a new perspective for exploring the mechanism of buffalo follicular dominance and improving buffalo reproductive performance.

Introduction: Access to veterinary services is integral for animals of all species. These services play a crucial role in maintaining their health and welfare and maintaining a healthy, safe, and sustainable food system. Research has consistently shown that rural communities face challenges accessing veterinary services, with livestock producers outlining several barriers including cost, inadequate infrastructure, and delays in receiving treatments. Research on bison producers' access to veterinary services is limited, prompting our investigation to address this gap in knowledge. This qualitative study aimed to describe Ontario bison producers' current access to veterinary services and identify how any barriers, as perceived by producers, might impact their herd health and management practices. Methods: Ontario bison producers were invited to participate in virtual focus groups to share their perspectives on their access to veterinary services. Audio from the focus groups was recorded, transcribed verbatim, and analyzed using reflexive thematic analysis. Results: Despite all participants indicating they had access to veterinary services, they also encountered obstacles and expressed concerns accessing and utilizing these services. Two overarching themes were identified: producers were concerned about the future stability and costs associated with bison farming, and they had a desire to improve bison-specific knowledge among veterinarians servicing their farms. Producers suggested several strategies to address these concerns, including improving collaborations with veterinary organizations, like the College of Veterinarians of Ontario, to increase veterinarians' exposure to bison, building stronger relationships between producers and their veterinarians, monetary incentives for established food animal veterinarians, and providing financial support to prospective food animal veterinarians. Discussion: The findings of this study demonstrate that although bison producers in our sample had access to veterinarians, they may not fully utilize the services or find their access entirely beneficial. Future research into the veterinarian perspective would allow for greater insights into these barriers, adding additional value and contributing to a more wholistic understanding of the topic. [ABSTRACT FROM AUTHOR]

This experiment was carried out to evaluate the effect of dietary supplementation of three different tropical seaweed species on rumen fermentation, methane emission, antioxidant status, immunity and milk production in lactating Murrah buffaloes. Twenty-four lactating Murrah buffaloes were divided into four groups of six each in an experiment based on randomized block design (RBD) and were fed to meet their nutrient requirements (ICAR 2013). Animals in control (CON) group were fed basal diet without any supplemental seaweed, however, Kappaphycus alvarezii (KA), Gracilaria salicornia (GS) and Turbinaria conoides (TC) were supplemented at 1% of the dietary dry matter in KA, GS and TC groups, respectively. Intake and apparent digestibility of nutrients, plasma concentrations of selected blood metabolites, and thyroid hormones were similar among the groups. Supplementation of KA and GS, but not TC increased (P < 0.001) the proportion of propionate in rumen fluid with a concurrent decrease (P < 0.001) in acetate: propionate. The enteric methane emission was lower (P < 0.05) in KA, GS groups and the maximum values were observed in groups CON and TC. Total antioxidant capacity (TAC) and immune response (cell-mediated and humoral) were higher (P < 0.001), and MDA was lower (P < 0.001) in seaweed-fed groups as compared to CON; the higher response was observed in group KA, followed by TC and GS. Plasma concentration of cortisol was lower (P < 0.001) in group KA as compared to other groups. Milk yield and the 6% fat-corrected milk (FCM) yield (kg day−1) were higher (P = 0.009) in KA, followed GS, corresponding values in group TC were not significantly different than those of group CON. Thus, supplementation of the tropical red seaweed improved antioxidant status, cellular and humoral immunity, and milk yield; the greater response was obtained when KA was used as feed supplement. It is concluded that supplementation of K. alvarezii at 1% of dietary DM of lactating Murrah buffaloes would improve antioxidant status, immunity and milk yield with reduction in enteric methane emission. [ABSTRACT FROM AUTHOR]

Since the late 1800s, north Australian savannas have experienced a fundamental shift in grazing pressure, moving from an assemblage of native macropods to introduced ungulates. In contrast to macropods, ungulates have hard hooves, are greater in size and exhibit bulk feeding strategies, resulting in a greater impact on perennial grass cover. However, the response of perennial grasses to ungulate grazing may vary depending on rainfall, soil fertility and fire, as well as ungulate density and feeding behaviour. Interactions between grazing, drought and fire are of particular interest, as climate change is predicted to increase rainfall variability and fire frequency across northern Australia. In this study, we tested for interactions between grazing and drought, as well as fire and grazing, on two vegetation zones surrounding waterholes, through a 5 years fencing exclusion experiment. Despite drought conditions occurring over 2 years of the study, no interaction between grazing and drought was detected. This is most likely due to the relatively low ungulate density present during the study (~1 ungulate/km2). In contrast, after fire was introduced to the study sites, significantly more perennial grass cover was recorded at fenced sites relative to unfenced sites. Due to the study design the cause of change cannot be directly inferred. However, when the potential drivers are considered further (rainfall, soil properties, fire and changes in ungulate density), an interaction between grazing and fire is the most probable cause. Wetland vegetation showed a stronger response to fencing than the surrounding savanna, with this result most likely due to differences in ungulate feeding behaviour between the two vegetation zones. Our findings are important, as fire and ungulate grazing are both widespread across Australia's savannas and reduced perennial grass cover has been linked to increased cat predation on rapidly declining small, native mammal populations. [ABSTRACT FROM AUTHOR]

Spermatogenesis is a highly complex and tightly regulated cellular differentiation process closely related to the productive performance of male livestock. We do not yet have a clear understanding of the spermatogenesis mechanism of buffalo. In this study, spermatogonia, spermatocytes and spermatids were analysed by flow cytometry. Quantitative proteomic and phosphoproteomic studies were performed on different spermatogenic cells using tandem mass tagging technology and liquid chromatography–tandem mass spectrometry. A total of 219 differentially expressed proteins (involved in focal adhesions and actin cytoskeleton pathways) and 71 phosphoproteins (involved in RNA transport and adhesion junction pathways) were obtained. Through trend analysis, a dynamic profile of protein expression was obtained, enriched to the main biological processes at different stages of spermatogenesis. By immunohistochemical localisation analysis, it was found that MACROH2A2, TOP2A, LMNA, LMNA (pS392), VIM and VIM (pS56) had specific localisation in testis cells. Network analysis of kinase‐substrate phosphorylation sites showed that AKT1 is the most active kinase, LMNA is regulated by most kinases and AKT1 can catalyse the phosphorylation of LMNA. This study provides a reference for studying the molecular mechanism of buffalo spermatogenesis and helps clarify the regulatory mechanism of protein translation and post‐translational modification during mammalian spermatogenesis. [ABSTRACT FROM AUTHOR]

Lipids in cumulus–oocyte complexes are important actors in molecular signalling pathways and are influenced by maturation conditions. Acetyl‐L‐carnitine (ALC) is a carrier involved in fatty acid transport and is a promoter of β‐oxidation. Although the embryonic development potential of oocytes can be improved when ALC is added to the maturation medium, the effects of ALC on the lipid content and composition of oocytes and cumulus cells remain unknown. In this study, the effect of ALC supplementation on the lipid profiles of buffalo oocytes and cumulus cells after in vitro maturation was evaluated using positive‐ion matrix‐assisted laser desorption ionisation time‐of‐flight mass spectrometry (MALDI‐TOF MS). Orthogonal projections to latent structures discriminant analysis was used to analyse the data. The lipid profiles of oocytes and cumulus cells differed following ALC treatment. Four lipids were significantly different in oocytes and eight in granulosa cells. ALC treatment significantly reduced the cellular content of these lipids, with the exception of phosphatidylcholine [PC(36:3) + H] + in oocytes and triacylglycerol [TAG(58:8) + NH4] + in granulosa cells. Further staining experiments confirmed that ALC treatment reduced the total lipid content in oocytes. Therefore, ALC treatment alters lipid metabolism in oocytes and cumulus cells during their maturation and accelerates lipid metabolism in oocytes. The findings of this study demonstrate that ALC enhances the rate of oocyte maturation by altering lipid metabolism in oocytes, providing both a clear direction for further research into regulatory mechanisms as well as a foundation for further improving oocyte maturation rates. [ABSTRACT FROM AUTHOR]

Background: The dairy and livestock sector plays a very important role in national economy of India by contributing close to one third of gross income of rural households and nearly half of gross income in case of those without land. Subestrus is the most prevalent, frustrating and challenging problem encountered in postpartum buffaloes. Postpartum subestrus is one of the most prevalent, frustrating and challenging reproductive problem encountered in rural resulting into prolonged inter-calving period, reduced milk production and thus greatly affecting the economy of our farming community. Methods: The present study was carried out on Graded Murrah buffaloes during the period from September 2017 to August 2019 (includes breeding and low breeding season) which were maintained at farm and field. Treatment of sub-estrus lactating graded Murrah buffaloes (Bubalus bubalis) using Double PGF2 α (DPG) and Presynch-Ovsynch (POVS) their influence on fertility in farm and field condition during breeding and low breeding season was studied. Total cholesterol and progesterone concentration was recorded during different days/period of protocols. Further, overall per cent conception and pregnancy rates were recorded and compared between two protocols. Result: Progesterone levels increased significantly on day 21 post AI in pregnant buffaloes, whereas decreased significantly in non-pregnant buffaloes both in farm and field during breeding and low breeding season. The overall per cent conception and pregnancy rates were higher in POVS group than DPG group at both farm and field as well as during breeding and low breeding seasons. [ABSTRACT FROM AUTHOR]

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Background/Objectives: Milk is one of the main sources of nutrition in people's daily diet, but the fat in milk raises health concerns in consumers. Here, we aimed to elucidate the impact of Buffalo milk and Holstein cow milk consumption on blood lipid health through metabolomics analysis. Methods: Golden hamsters were administered Murrah Buffalo milk (BM) or Holstein cow milk (HM), and the body weight and serum lipid indicators were tested and recorded. The hamsters receiving equal amounts of physiological saline were used as the negative control (NC). Serum and fecal samples were collected, and LC-MS was used to identify the metabolites in the samples. Results: The results showed that both the BM and HM groups exhibited a significant reduction in body weight compared to that of the NC group from day 9, and the serum TG, TC, and LDL-C levels were significantly lower than those of the NC group. Further analysis identified 564 and 567 metabolites in the serum and fecal samples shared in the BM and HM groups and significantly different from those in the NC group, which were mainly enriched in the pathways related to lipid metabolism, such as fatty acid biosynthesis, arachidonic acid metabolism, and primary bile acid biosynthesis. Correlation analysis further suggested that milk intake can increase the levels of Muramic Acid, Oleoyl Ethanolamide, Seratrodast, Chenodeoxycholic Acid, Docosahexaenoic Acid Ethyl Ester, and Deoxycholic Acid in the serum and gut microbiota, which may affect TG, TC, HDL-C, and LDL-C in the serum, and thereby benefit the body's lipid health. Conclusions: The results further confirmed that milk intake has a beneficial effect on blood lipid health by altering multiple metabolites in the serum and the gut. This study provides novel evidence that milk consumption is beneficial to health and is a reference for guiding people to a healthy diet. [ABSTRACT FROM AUTHOR]

Rectal thermometers are commonly used to assess animal health, but non-invasive methods like thermal imaging are gaining popularity for their ease. This study explores using infra-red thermography to measure eye temperatures as a proxy for body temperature in buffaloes. Among 280 adult female buffaloes, rectal temperature averaged 38.26 ± 0.38 °C, while eye temperature averaged 36.99 ± 0.47 °C. Statistical analysis showed a significant positive correlation (0.674, p < 0.001) between eye and rectal temperatures in adult buffaloes. Moderate correlations were also found between rectal temperature and ambient temperature (0.488, p < 0.001), as well as with ocular temperature (0.488, p < 0.001), while the correlation with humidity was low (0.176, p < 0.05). Regression modelling incorporating rectal and eye temperatures, ambient temperature, and humidity revealed that ocular temperature and ambient temperature significantly affect rectal temperature. The developed equation RT ̂ = 20.377 + 0.465(AET) + 0.024(AT) accurately predicts rectal temperature in buffaloes, with R2 of 51.6%, RMSE = 0.272, and AIC = 71.601 indicating satisfactory variability. These findings highlight the potential of thermal imaging as a diagnostic tool and provide insights into temperature measurement interrelationships. HIGHLIGHTS: The study highlights infra-red thermal imaging as a non-invasive technique for remotely measuring the core temperature of buffaloes. There is a significant and high correlation (p < 0.001) between rectal temperature (RT) and average eye temperature (AET) in buffaloes, indicating proportional shifts in AET with changes in RT. The relationship between rectal temperature (RT) and average eye temperature (AET) is quantified by the equation RT = 20.377 + 0.465(AET) + 0.024(AT), derived from a regression model with R2 of 51.6% and absence of autocorrelation (Durbin-Watson = 1.318), where AT represents ambient temperature. [ABSTRACT FROM AUTHOR]

Buffalo is a rustic animal; it can adapt and survive in various environments with different topography, climate and vegetation, and has a greater longevity than cattle. The high resistance to parasites, as well as the ability to improve the efficiency of feed consumption in case of forage shortage, means that even in marshy territories, where any other type of livestock or agricultural activity is impossible, buffalo farming can develop an economy. This review aims to present the key research topics, the evolution and geographic distribution of literature on buffalo welfare, and to underscore potentiality and existing knowledge gaps. A comprehensive search on the Scopus bibliometric database was carried out using various welfare-related keywords such as: 'buffalo welfare', 'buffalo calf welfare' and 'buffalo heifers' welfare'. The research identified 308 papers published in English from January 1992 to December 2023 that, after filtering, became 148 eligible records. Descriptive statistics revealed a significant exponential increase in studies on buffalo welfare since 2020, particularly in Italy, followed by Brazil. Text mining and topic analysis revealed that the most frequently mentioned terms were 'milk', 'water', 'behaviour', 'farm' and 'stun', confirming the role and the importance of this species in dairy production. Nine identified topics span diverse subject areas such as 'slaughter process', 'dairy production', 'general welfare', 'housing system', 'heat stress', 'reproduction performance', 'health status' 'breeding management', and 'stress evaluation'. Although academia shows an increasing interest in buffalo behaviour and welfare, continued research is necessary to fully comprehend how to ensure the welfare of this important species. HIGHLIGHTS: Overview of buffalo welfare in scientific literature Usage of new techniques as text mining and topic analysis for the review process Slaughter process and Dairy production were the most frequent topics [ABSTRACT FROM AUTHOR]

The current study aimed to construct the growth curve and find the best-fitting non-linear model for the Nili-Ravi buffalo calves. The pedigree and monthly body weight data for 6644 calves born between 2010 and 2020 (inclusive) in six herds, maintained in different geographical regions of Punjab were collected. The study was performed under a longitudinal design and various non-linear models were used to associate the age with weight by using the easyreg package in R studio. Finally, the genetic parameters of growth curve were estimated through the bi-variate sire model in ASReml (v4.2). The model with the highest value of coefficient of determination and the lowest values of Akaike’s information criterion, Bayesian information criterion, and root-mean-square error was considered as the best fit for defining the growth curve. The Brody model was found as the best fitted model with the values of 0.6648, 627871.80, 627908.10, and 30.793 for the R2adj, AIC, BIC, & RMSE respectively, for the combined dataset. The values of growth curve parameters for the Brody model were 943.99 ± 101.38Kg (A), 0.96 ± 0.004Kg (B), and 0.0005 ± 0.00Kg (K) for all animals. A higher K-value of females indicates their early maturity compared with male animals in this breed. The heritability estimates for the growth curve traits were low, while the values of genetic correlations were higher than those of phenotypic correlations. The data revealed that Asymptotic weight (A) and birth weight (B) were positively correlated with each other, while the rate of maturity (K) was negatively correlated with initial and final body weights. [ABSTRACT FROM AUTHOR]

Background: Indigenous buffaloes, as the important livestock species contributing to economy of the country, are the lifeline of livelihood in Nepal. They are distributed across diverse geographical regions of the country and have adapted to various feeding, breeding, and management conditions. The larger group of these native buffalo breeds are present in narrow and stiff hilly terrains. Their dispersal indicates a possible environmental adaptation mechanism, which is crucial for the conservation of these breeds. Results: We utilized whole-genome sequencing (WGS) to investigate the genetic diversity, population structure, and selection signatures of Nepalese indigenous buffaloes. We compared 66 whole-genome sequences with 118 publicly available sequences from six river and five swamp buffalo breeds. Genomic diversity parameters indicated genetic variability level in the Nepalese buffaloes comparable to those of Indian breeds, and population genetic structure revealed distinct geography-mediated genetic differentiation among these breeds. We used locus-specific branch length analysis (LSBL) for genome-wide scan, which revealed a list of potentially selected genes in Lime and Parkote breeds that inhabit the hilly region. A gene ontology (GO) analysis discovered that many GO terms were associated with cardiac function regulation. Furthermore, complementary analyses of local selection signatures, tissue expression profiles, and haplotype differences identified candidate genes, including KCNE1, CSF1R, and PDGFRB, related to the regulation of cardiac and pulmonary functions. Conclusions: This study is a comprehensive WGS-based genetic analysis of the native Nepalese buffalo breeds. Our study suggested that the Nepalese "hilly" buffaloes, especially the Lime and Parkote breeds, have undergone some characteristic genetic changes and evolved increased cardiac and pulmonary function for their adaptation to the steep hilly terrains of the country. [ABSTRACT FROM AUTHOR]

In mammals, sex determination is governed by the SRY gene on the Y chromosome, redirecting gonadal development from forming ovaries to testes. Mutations or alterations in the SRY gene can significantly affect phenotypic changes and lineage‐specific markers. This study aims to elucidate the role of the SRY gene in buffalo embryos using CRISPR‐Cas9 technology. We designed a crRNA targeting the HMG domain of the SRY gene using the CRISPOR algorithm. Nucleofection of sgRNA‐Cas9 RNPs into buffalo fibroblasts confirmed efficient cleavage at the targeted site. Using this validated guide, we investigated the role of the SRY gene in sexual determination by electroporating CRISPR‐Cas9‐RNPs into single‐stage zygotes of buffalo. Genetic changes in the SRY gene were confirmed through sequencing, revealing mosaic blastocysts with multiple alleles and non‐mosaic mutants. Mutations in SRY gene increased the expression of female lineage‐specific gene Wnt4 whereas decreased the expression of male specific gene SOX9 in blastocysts, suggesting reprogramming towards female sex determination pathways. Our findings provide insights into buffalo sex differentiation mechanisms and potential applications in reproductive strategies for breeding programmes. [ABSTRACT FROM AUTHOR]

The PPARGC1A gene plays a fundamental role in regulating cellular energy metabolism, including adaptive thermogenesis, mitochondrial biogenesis, adipogenesis, gluconeogenesis, and glucose/fatty acid metabolism. In a previous study, our group investigated seven SNPs in Mediterranean buffalo associated with milk production traits, and the current study builds on this research by exploring the regulatory influences of the PPARGC1A gene in buffalo mammary epithelial cells (BuMECs). Our findings revealed that knockdown of PPARGC1A gene expression significantly affected the growth of BuMECs, including proliferation, cell cycle, and apoptosis. Additionally, we observed downregulated triglyceride secretion after PPARGC1A knockdown. Furthermore, the critical genes related to milk production, including the STATS, BAD, P53, SREBF1, and XDH genes were upregulated after RNAi, while the FABP3 gene, was downregulated. Moreover, Silencing the PPARGC1A gene led to a significant downregulation of β-casein synthesis in BuMECs. Our study provides evidence of the importance of the PPARGC1A gene in regulating cell growth, lipid, and protein metabolism in the buffalo mammary gland. In light of our previous research, the current study underscores the potential of this gene for improving milk production efficiency and overall dairy productivity in buffalo populations. [ABSTRACT FROM AUTHOR]

This study aimed to investigate and characterize the spermatogonial stem cells (SSCs) in buffaloes at different stages of development, including prenatal, neonatal, prepubertal, and adult testes. We sought a comprehensive understanding of these cells through a combination of histological, immunohistochemical, and ultrastructural analyses. Specifically, we examined changes in the expression of two potential SSC markers, OCT4 and PGP9.5, using immunohistochemistry. Additionally, we conducted a real-time quantitative polymerase chain reaction (RT-qPCR) to assess the relative gene expression of OCT4 and PGP9.5. The relative expression of the OCT4 gene was down-regulated in the adult testes compared to its expression during prepubertal and neonatal life. The relative expression of the PGP9.5 gene was up-regulated in the neonatal testes and down-regulated in the prepubertal and adult testes. The spermatogonia were round, oval-to-ellipsoidal cells lying over the basement membrane (BM) with a round-to-oval nucleus. Based on the immunoexpression of the putative SSC markers, OCT4 and PGP9.5, we concluded that the proportion of stem cells was highest during the neonatal stage, followed by the prepubertal and prenatal stages. This finding sheds light on the dynamics of spermatogonial stem cells in buffalo testes at different developmental stages, providing valuable insights into these cells' regulation and potential applications. [ABSTRACT FROM AUTHOR]

The main objective of the study is to investigate and determine the factors affecting the productivity of the seed obtained from buffalo germplasm. In the research work, different factors affecting the productivity of the seed were identified. These are genotype, fodder and feeding systems, buffalo feeding. [ABSTRACT FROM AUTHOR]

Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis, an oncogenic deltaretrovirus that has emerged as a potential zoonotic infection. The BLV naturally infects cattle and causes economic losses through a slow persistent infection with various clinical symtoms following preleukosis. The main objective of this study was to determine the seroprevalence of BLV antibodies in cattle and buffaloes in the border provinces of the Eastern Anatolia region, Türkiye, using the agar gel immunodiffusion (AGID) assay and enzyme-linked immunosorbent assay (ELISA). For this purpose, a total of 1,033 serum samples were collected from 982 cattle and 51 buffaloes from the provinces of Ağrı (n = 178), Iğdır (n = 252), Kars (n = 317), Van (n = 221), and Hakkari (n = 65) during 2021 - 2022. In AGID and ELISA tests, seropositivity for BLV-specific antibodies was not detected in cattle and buffaloes from the mentioned provinces. This study revealed that BLV was not circulating in cattle and buffaloes in the easternmost border provinces of Türkiye during the sampling period and contributed to determine the status of BLV in the mentioned region. Due to the presence of virus in other regions of Türkiye and neighboring countries, Iran and Iraq, it is recommended to control animal movements, continue efforts to combat the transmission of the virus, and maintain control measures. [ABSTRACT FROM AUTHOR]

Herein, we evaluated the effects of gonadotropin hormone-releasing hormone (GnRH) administration 84 h after medroxyprogesterone acetate (MAP) sponge removal on follicular growth, ovulation timing, and pregnancy per artificial insemination (AI) in cosynchronized postpartum Nili Ravi buffaloes. In this study, 58 Nili Ravi postpartum buffaloes (DIM = 103 ± 1.64) were randomly divided into two treatment groups (n = 29/treatment): GnRH-TAI-84 and TAI-84. All buffaloes were administered a MAP sponge for seven days. Upon MAP sponge removal, all the subjects received prostaglandin F2a (PGF2a) and Timed AI (TAI) was performed 84 h after sponge removal. In the GnRHTAI-84 group, the buffaloes received GnRH alongside insemination, whereas in the TAI-84 group, the buffaloes were inseminated without GnRH administration. Follicle diameter and blood estradiol levels were measured every 6 h from 72-108 h after MAP sponge removal. The animals were checked for pregnancy using ultrasonography 40 days after AI. Animals subjected to the GnRH-TAI-84 protocol had a higher follicular growth rate and preovulatory follicle size than those in the TAI-84 group. The follicular diameter was also larger in animals that received GnRH-TAI-84 than in those that received TAI-84 90 and 96 h after MAP sponge removal. Buffaloes in the GnRH-TAI-84 group had lower estradiol concentrations at 90, 96, 102, and 108 h than those in the TAI-84 group. Ovulation in GnRHTAI-84 buffaloes occurred 11 h earlier than that in buffaloes from the TAI-84 group. A shorter interval between AI and ovulation in GnRH-TAI-84 buffaloes (14 h vs. 25 h) led to greater pregnancies per AI (62% vs. 17%) compared to buffaloes from the TAI-84 group. [ABSTRACT FROM AUTHOR]

Background: Buffalo is a globally important livestock species, but its reproductive performance is relatively low than cattles. At present, dominant follicle development specific process and mechanistic role of follicular growth related genes in water buffaloes are not well understood. Therefore, we comprehensively performed transcriptomics of granulosa cells and oocytes from different-sized follicles in water buffalo to identify key candidate genes that influence follicle development and diameter, and further explored the potential regulatory mechanisms of granulosa cells and oocytes in the process of water buffalo follicle development. Results: In this study, we found918 granulosa cell transcripts and 1401 oocyte transcripts were correlated in follicles of different diameters, and the expression differences were significant. Subsequent enrichment analysis of the co-expressed differentially expressed transcripts identified several genes targeted by long non-coding RNAs (lncRNAs) and associated with follicular development. Notably, the upregulation of BUB1 regulated by MSTRG.41325.4 and interactive action of SMAD2 and SMAD7 might have key regulatory role in follicular development. Additionally, we also detected key differentially expressed genes that potentially influence follicular hormone metabolism and growth, like ID2, CHRD, TGIF2 and MAD2L1, and constructed an interaction network between lncRNA transcripts and mRNAs. Conclusions: In summary, this study preliminarily revealed the differences in gene expression patterns among buffalo follicles of different sizes and their potential molecular regulatory mechanisms. It provides a new perspective for exploring the mechanism of buffalo follicular dominance and improving buffalo reproductive performance. [ABSTRACT FROM AUTHOR]

Mother–offspring communication is especially crucial for social species in order to synchronize activities essential for early survival including nursing, resting, maintaining proximity during group movements between food or water sources, and locating one another if separated in a large social group. One of the most social ungulate species in North America is the American Bison (Bison bison), formerly known as buffalo. Adult female bison associate with their young for over a year and communication between mother and offspring is likely essential for establishing and maintaining a bond upon which the life of a calf depends. One goal of this study was to quantify and compare the acoustic form of vocalizations of adult female, subadult, and calf bison and to determine how age classes differed in call structure. The other goal was to identify the contexts in which bison vocalized. Vocalizations of 101 bison (53 adult females, 15 subadults, 33 calves) in a semi-free-ranging herd in Montana were analyzed and found to be pulsatile sounds, unlike vocalizations of bison bulls or domestic cows and calves. Vocalizations of bison cows, subadults, and calves differed significantly in total duration, numbers of pulses, pulse duration, and pulse rate. Seven distinct call contexts were identified. The majority of calls were "moving-on calls" (39%), when a cow called and her calf ran to her side and the 2 moved on together, and "contact calls" (21%) when a cow called and her calf called back but neither changed their location. "Imprinting calls" and "nursing calls" were also identified. Mother–offspring acoustic communication in bison appears especially critical for coordinating movements. Understanding the role of acoustic communication in maintaining the bond between bison mothers and their offspring can contribute to the humane management and welfare of this iconic species. [ABSTRACT FROM AUTHOR]