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NaVPO4F has become a focal point in the realm of sodium-ion battery cathode materials owing to its robust structure, elevated voltage platform, and considerable theoretical capacity. Nonetheless, the limited conductivity of NaVPO4F poses a challenge to its practical utilization. In this study, we introduce a straightforward one-step ball milling process to address this issue by creating NaVPO4F/G (G stands for graphite) and enhancing electronic conductivity through the incorporation of polyethylene glycol 6000 (PEG6K). Graphite has the capability to enhance electrochemical properties and markedly enhance electronic conductivity. Furthermore, the incorporation of PEG6K can enhance the transport stability of sodium ions at the interface of sodium-ion batteries. After optimization, NaVPO4F/G@P (P stands for PEG6K) delivers a high capacity of 128.5 mA h g−1 at 0.2C and a high rate capacity of 105.0 mA h g−1 at 10C, with a capacity retention rate of 116.0 mA h g−1 after 200 cycles. Moreover, when coupled with a HC anode (HC as the negative electrode), the full cell still displays an outstanding performance of 76.0 mA h g−1 at 20C. A simple approach is provided in our study that has the potential to significantly improve the performance of sodium-ion batteries. [ABSTRACT FROM AUTHOR]

A straightforward carbothermal reduction method was used to synthesize nitrogen-doped carbon-coated LiFePO4 with the carbon and nitrogen sources derived from gelatin. Compared to the bare material, the particles of LiFePO4 composites are smaller, the electrical contact between the grains is improved, and lithium ions diffuse more rapidly in the composites. As a consequence, the structural stability and conductivity are enhanced in LiFePO4 compounds. After 500 cycles, the material delivered 138.2 mA h g−1 at a multiplicity of 1C with 90.2% capacity retention. This result demonstrates that a suitable amount of N-doped carbon layer can enhance the electrochemical performance of LiFePO4. This efficient strategy provides a facile approach to develop excellent performance LiFePO4 materials with promising practical applications. [ABSTRACT FROM AUTHOR]

The detailed studies of converted S-waves recorded on the Ocean Bottom Seismometer (OBS) can provide evidence for constraining lithology and geophysical properties. However, the research of converted S-waves remains a weakness, especially the S-waves' inversion. In this study, we applied a travel-time inversion method of converted S-waves to obtain the crustal S-wave velocity along the profile NS5. The velocities of the crust are determined by the following four aspects: (1) modelling the P-wave velocity, (2) constrained sediments Vp/Vs ratios and S-wave velocity using PPS phases, (3) the correction of PSS phases' travel-time, and (4) appropriate parameters and initial model are selected for inversion. Our results show that the vs. and Vp/Vs of the crust are 3.0–4.4 km/s and 1.71–1.80, respectively. The inversion model has a similar trend in velocity and Vp/Vs ratios with the forward model, due to a small difference with ∆Vs of 0.1 km/s and ∆Vp/Vs of 0.03 between two models. In addition, the high-resolution inversion model has revealed many details of the crustal structures, including magma conduits, which further supports our method as feasible. [ABSTRACT FROM AUTHOR]

Prostate cancer (PCa) is the second leading cause of cancer‐related death in males, primarily due to its metastatic potential. The present study aims to identify the expression of microRNA‐539 (miR‐539) in PCa and further investigate its functional relevance in PCa progression both in vitro and in vivo. Initially, microarray analysis was conducted to obtain the differentially expressed gene candidates and the regulatory miRNAs, after which the possible interaction between the two was determined. Next, ectopic expression and knock‐down of the levels of miR‐539 were performed in PCa cells to identify the functional role of miR‐539 in PCa pathogenesis, followed by the measurement of E‐cadherin, vimentin, Smad4, c‐Myc, Snail1 and SLUG expression, as well as proliferation, migration and invasion of PCa cells. Finally, tumour growth was evaluated in nude mice through in vivo experiments. The results found that miR‐539 was down‐regulated and DLX1 was up‐regulated in PCa tissues and cells. miR‐539 was also found to target and negatively regulate DLX1 expression, which resulted in the inhibition of the TGF‐β/Smad4 signalling pathway. Moreover, the up‐regulation of miR‐539 or DLX1 gene silencing led to the inhibition of PCa cell proliferation, migration, invasion, EMT and tumour growth, accompanied by increased E‐cadherin expression and decreased expression of vimentin, Smad4, c‐Myc, Snail1 and SLUG. In conclusion, the overexpression of miR‐539‐mediated DLX1 inhibition could potentially impede EMT, proliferation, migration and invasion of PCa cells through the blockade of the TGF‐β/Smad4 signalling pathway, highlighting a potential miR‐539/DLX1/TGF‐β/Smad4 regulatory axis in the treatment of PCa. [ABSTRACT FROM AUTHOR]

The southernmost Mariana margin lacks a mature island arc and thus differs significantly from the central‐north Mariana and Izu‐Bonin margins. This paper presents a new P wave velocity model of the crust and uppermost mantle structure based on a 349‐km‐long profile of wide‐angle reflection/refraction data. The active source seismic experiment was conducted from the subducting Pacific plate to the overriding Philippine plate, passing through the Challenger Deep. The subducting plate has an average crustal thickness of ~6.0 km with Vp of 7.0 ± 0.2 km/s at the base of the crust and low values of only 5.5–6.9 km/s near the trench axis. The uppermost mantle of the subducting plate is characterized by low velocities of 7.0–7.3 km/s. The overriding plate has a maximum crustal thickness of ~18 km beneath the forearc with Vp of ~7.4 km/s at the crustal bottom and 7.5–7.8 km/s in the uppermost mantle. A zone of slight velocity reduction is imaged beneath the Southwest Mariana Rift that is undergoing active rifting. The observed significant velocity reduction in a near‐trench crustal zone of ~20–30 km in the subducting plate is interpreted as a result of faulting‐induced porosity changes and fracture‐filling fluids. The velocity reduction in the uppermost mantle of both subducting and overriding plates is interpreted as mantle serpentinization with fluid sources from dehydration of the subducting plate and/or fluid penetration along faults. Plain Language Summary: The southernmost Mariana margin is a convergence margin that lacks a mature island arc. The trench reaches the deepest point on Earth at the Challenger Deep, the origin of which is still little known. We acquired and analyzed new active source wide‐angle reflection/refraction seismic data along a profile across the Challenge Deep, obtaining a new model of crustal and uppermost mantle velocity structure of both the subducting Pacific and overriding Philippine Sea plates. Significant velocity reduction is observed in the crust of the subducting plate, especially in a 20‐ to 30‐km‐wide zone near the trench axis, which is interpreted as a result of faulting‐induced porosity changes and fracture‐filling fluids. A zone of slight velocity reduction is imaged beneath the tectonically active Southwest Mariana Rift. Significant velocity reduction is also observed in the uppermost mantle of both subducting and overriding plates and is interpreted as a result of mantle serpentinization. Key Points: We conducted an active‐source seismic experiment across the Challenger Deep, revealing a new velocity model of the southernmost Mariana marginReduction in crustal velocity was observed in the subducting plate, especially in a near‐trench zone, possibly due to porosity changes and fluids; Relatively low upper mantle velocities were interpreted as mantle serpentinizationA low‐velocity zone of ~15‐ to 20‐km‐wide was imaged beneath the Southwest Mariana Rift, interpreted as a weak zone of initial rifting [ABSTRACT FROM AUTHOR]

Expression of peroxisome proliferator-activated receptor γ (PPARγ) mRNA in ovarian granulosa cells of patients with polycystic ovary syndrome (PCOS) were explored. Ovarian granulosa cells were extracted from 5 patients with PCOS and 30 normal controls. Expression of PPARγ mRNA in granulosa cells of the 5 PCOS patients (observation group) and 5 normal controls (control group) was detected by RT-qPCR. The remaining 25 cases of normal human ovarian granulosa cells were cultured in vitro for 48 h, followed by cell culture for another 24 h with different concentrations of testosterone, insulin (INS), and rosiglitazone (RGZ). After that, expression of PPARγ mRNA was detected by RT-qPCR. Relative expression level of PPARγ mRNA in the observation group was significantly lower than that in the control group. Compared with testosterone concentration at 10-2 mol/ml, testosterone concentration at 10-3 mol/ml significantly reduced the expression level of PPARγ mRNA. When the INS concentration was 10-5 mol/ml, relative expression level of PPARγ mRNA was significantly higher than that of the control group (P<0.01). Relative expression level of PPARγ mRNA was significantly higher than that of the control group when INS concentration was 10-4 mol/ml. When the concentration of RGZ was 10-4 mol/ml, the relative expression level of PPARγ mRNA was significantly higher than that of the control group (P<0.01). When concentration of RGZ was 10-3 mol/ml, expression level of PPARγ mRNA was significantly increased comparing to that under a RGZ concentration of 10-4 mol/ml or that of the control group (P<0.01). Appropriate concentrations of testosterone can inhibit the expression of PPARγ mRNA in ovarian granulosa cells, and certain concentrations of INS and RGZ can induce the expression of PPARγ mRNA in ovarian granulosa cells. Abnormal expression of PPARγ mRNA in ovarian granulosa cells may be related to the mechanism of PCOS. [ABSTRACT FROM AUTHOR]

The Pearl River Estuary (PRE) is located at the onshore-offshore transition zone between South China and South China Sea Basin, and it is of great significant value in discussing tectonic relationships between South China block and South China Sea block and seismic activities along the offshore active faults in PRE. However, the researches on geometric characteristics of offshore faults in this area are extremely lacking. To investigate the offshore fault distribution and their geometric features in the PRE in greater detail, we acquired thirteen seismic reflection profiles in 2015. Combining the analysis of the seismic reflection and free-air gravity anomaly data, this paper revealed the location, continuity, and geometry of the littoral fault zone and other offshore faults in PRE. The littoral fault zone is composed of the major Dangan Islands fault and several parallel, high-angle, normal faults, which mainly trend northeast to northeast-to-east and dip to the southeast with large displacements. The fault zone is divided into three different segments by the northwest-trending faults. Moreover, the basement depth around Dangan Islands is very shallow, while it suddenly increases along the islands westward and southward. These has resulted in the islands and neighboring areas becoming the places where the stress accumulates easily. The seismogenic pattern of this area is closely related to the comprehensive effect of intersecting faults together with the low velocity layer. [ABSTRACT FROM AUTHOR]

The northern margin of the South China Sea, as a typical extensional continental margin, has relatively strong intraplate seismicity. Compared with the active zones of Nanao Island, Yangjiang, and Heyuan, seismicity in the Pearl River Estuary is relatively low. However, a M4.0 earthquake in 2006 occurred near Dangan Island (DI) offshore Hong Kong, and this site was adjacent to the source of the historical M5.8 earthquake in 1874. To reveal the seismogenic mechanism of intraplate earthquakes in DI, we systematically analyzed the structural characteristics in the source area of the 2006 DI earthquake using integrated 24-channel seismic profiles, onshore-offshore wide-angle seismic tomography, and natural earthquake parameters. We ascertained the locations of NW- and NE-trending faults in the DI sea and found that the NE-trending DI fault mainly dipped southeast at a high angle and cut through the crust with an obvious low-velocity anomaly. The NW-trending fault dipped southwest with a similar high angle. The 2006 DI earthquake was adjacent to the intersection of the NE- and NW-trending faults, which suggested that the intersection of the two faults with different strikes could provide a favorable condition for the generation and triggering of intraplate earthquakes. Crustal velocity model showed that the high-velocity anomaly was imaged in the west of DI, but a distinct entity with low-velocity anomaly in the upper crust and high-velocity anomaly in the lower crust was found in the south of DI. Both the 1874 and 2006 DI earthquakes occurred along the edge of the distinct entity. Two vertical cross-sections nearly perpendicular to the strikes of the intersecting faults revealed good spatial correlations between the 2006 DI earthquake and the low to high speed transition in the distinct entity. This result indicated that the transitional zone might be a weakly structural body that can store strain energy and release it as a brittle failure, resulting in an earthquake-prone area. [ABSTRACT FROM AUTHOR]

We used earthquake catalogs recorded by Guangdong Seismological Network from 2008 to 2014 to resolve the spatial variations of b-values in the coastal area of Guangdong, particularly in three key research areas (Yangjiang, Heyuan, and offshore Nanao Island) with strong seismicity. Our results revealed that b-values exhibited significant spatial variations, and zones with low b-values could indicate the most likely seismogenic area of large earthquakes. We observed three clear low b-value patches in the offshore Nanao Island. We found a distinct high b-value peak at the depth of 11 km and two minimum peaks at about 14 and 7-8 km in the Yangjiang area. The overall b-values generally decrease with depth in the Heyuan area. The spatial variations of b-values reflect tectonic anomalies; that is, the 'low-high-low' distribution of b-values in the offshore Nanao Island and the Yangjiang area may indicate the anomaly of the crustal structure with a weak layer. The b-values of reservoir-induced seismicity are obviously lower than that induced by tectonism. This finding indicates that the reservoir area is generally at high stress state under the condition of high pore pressure. We inferred that large earthquakes might be prone to occur at 10-12 km depth in the offshore Nanao Island, at 12-15 km depth in the Yangjiang area, and at the lower part of the seismic activity zone in the Heyuan area. Moreover, the upstream area of the Xinfengjiang reservoir is the most likely area of future large earthquakes in the Heyuan area. [ABSTRACT FROM AUTHOR]

A cluster of earthquakes occurred in the Taiwan Shoal region on the outer rise of the Manila Trench. Although most were of small to medium magnitudes, one strong earthquake occurred on September 16, 1994. Several previous studies have provided important information to progress our understanding of this single earthquake. However, little is currently known about the earthquake cluster, and it is necessary to investigate the deep crustal structure of the Taiwan Shoal region to understand the mechanisms involved in controlling and generating it. This study presents a two-dimensional seismic tomographic image of the crustal structure along the OBS2012 profile based on ocean-bottom seismograph (OBS) data, which exhibits a high-velocity anomaly flanked by low-velocity anomalies in the upper crust beneath the Taiwan Shoal. In this study, 765 earthquakes (Richter magnitude M > 1.5) occurring between 1991 and 2015 were studied and analyses of earthquake epicenters, regional faults, and the crustal structure provides an improved understanding of the nature of active tectonics in this region. Results of analyses indicate firstly that the high-velocity area represents major asperities that correspond to the location of the earthquake cluster and where stress is concentrated. It is also depicted that the earthquake cluster was influenced by fault interactions. However, the September 1994 earthquake occurred independently of these seismic activities and was associated with reactivation of a preexisting fault. It is also determined that slab pull is resisted by the exposed precollision accretionary prism, and the resistive force is causing accumulation of inplane compressive-stress. This may trigger a future damaging earthquake in the Taiwan Shoal region. [ABSTRACT FROM AUTHOR]

OBJECTIVE. The objective of this study was to prepare, characterize, and determine immunological activities of specific transfer factor (STF) specific to human sperm antigen (HSA) for the preparation of antisperm contraceptive vaccine that can be used as an immunocontraceptive. METHODS. HSA-STF was prepared using the spleens of rabbits vaccinated with HSA. The specific immunological activities were examined by lymphocyte proliferation test (LPT), leukocyte adhesion inhibition test (LAIT), and by determining the concentrations of IL-4, ¿ -IFN, and IL-21. HSA-STF was a helveolous substance, having a pH value of 7.0 ± 0.4 and UV absorption maxima at 258 ± 6 nm. It contained seventeen amino acids; glycine and glutamic acids were the highest in terms of concentrations (38.8 ¿ g/mL and 36.3 ¿ g/mL, resp.). RESULTS. The concentration of polypeptide was 2.34 ± 0.31 mg/mL, and ribose was 0.717 ± 0.043 mg/mL. The stimulation index for lymphocyte proliferation test was 1.84, and the leukocyte adhesion inhibition rate was 37.7%. There was a statistically significant difference between the cultural lymphocytes with HSA-STF and non-HSA-STF for ¿ -IFN and IL-21 (P < 0.05), but there was no statistical significance for IL-4 (P > 0.05). CONCLUSION. HSA-STF was prepared and characterized successfully. It had immunological activity which could transfer the immune response specific to HSA and prove to be a potential candidate for the development of male immunocontraceptive agents. [ABSTRACT FROM AUTHOR]

Abstract: The Cenozoic tectonic subsidence of the deepwater area in the Pearl River Mouth Basin, northern South China Sea is studied by subsidence analysis via backstripping calculations based on data of newly interpreted sequence boundaries. In the subsidence analysis local porosity–depth parameters were estimated based on well data, the paleo-water depth parameters and lithology were estimated for all grid nodes based on well data and sedimentary system maps, and ages of sequence boundaries were adjusted according to the International Chronostratigraphic Chart v2013/01. Sensitivity analysis shows that these are essential to ensure the quality of the subsidence analysis in the slope areas. Maps of tectonic subsidence rates of 18 Cenozoic sequences were constructed and spatial–temporal variations of the tectonic subsidence were discussed. The subsidence was restricted in the Baiyun and Liwan Sags before 30Ma and extended to uplifts after 30Ma. This indicates that the 30Ma unconformity is the breakup unconformity that separated the syn-rift and post-rift sequences. Four substages of post-rift subsidence were identified by cyclic changes in the rate and pattern of tectonic subsidence at boundaries of 23.3Ma, 17.2Ma and 11.9Ma, respectively. Anomalous post-rifting tectonic subsidence was significant and totaled to ~1200m at the center of the Baiyun Sag, and punctuated by rapid subsidence and uplift events. The Baiyun Event was manifested in the study area by a short-lived uplift event in 23.9–23.3Ma followed by a short-lived rapid subsidence in 23.3–19.8Ma in the central study area. This localized rapid subsidence caused northward jump and anti-clockwise rotation of the shelf break. Two-order teeterboard (seesaw)-like subsidence was observed in the study area in the post-rifting stage, suggesting elastic deformations of the crust and lithosphere. [Copyright &y& Elsevier]

As a typical rifted margin, the coastal region of the northern South China Sea has experienced many large crustal earthquakes in the past 400 years. For example, two destructive earthquakes with ~M7.0 in 1600 and ~M7.5 in 1918 occurred close to the NanAo Island. However, the mechanism of these earthquakes and seismotectonics of the region are still unclear. In this work we make the first study of the detailed three-dimensional crustal structure in the source zone of the 1918 NanAo earthquake (M7.5) using newly acquired wide-angle seismic reflection data. Our results show that current seismicity mostly occurred in a midcrustal low-velocity zone or its nearby high-to-low velocity transitional areas. We ascertain locations of the ENE-strike Littoral fault zone and the NW-strike Huanggangshui fault based on the seismic reflection profiles. These faults have caused a large-scale vertical dislocation of the sedimentary basement. The structure of the upper crust is very heterogeneous, containing many high or low velocity anomalies. However, the lower crustal structure is less heterogeneous, mainly characterized by low velocity mostly in the northern part of the study area and high velocity mainly in the southern part. The crust in the seismicity zone shows strong heterogeneity, reflecting highly cracked structural features possibly caused by destructive earthquakes. We infer that the midcrustal low-velocity zone and the intersection between the Littoral fault zone and the Huanggangshui fault could reflect weak structures in the crust and form a local stress buildup, which strongly affected or controlled the generation of intraplate seismicity. The 1918 NanAo earthquake very likely occurred within the current seismicity zone with a depth range of 15–25 km estimated from our tomographic images and previous geological and geophysical results. • A 3D crustal model constrained by wide-angle seismic data is first imaged in the source area of the 1918 Nan'ao earthquake. • Highly cracked structural featuresinthe seismicity zone is revealed, which possibly caused by destructive earthquakes. • We think the midcrustal LVZ and fault intersection could form a local stress buildup to affect intraplate seismicity. [ABSTRACT FROM AUTHOR]

• A large-scale fault zone was identified on the northern South China Sea. • The fault zone accommodated intra-plate deformation during Cenozoic extension. • The fault zone was inherited from a lithospheric-scale suture zone. • The fault zone played a significant role in segmenting the northern SCS margin. Multibeam bathymetric, seismic and borehole data are used to investigate a large-scale strike-slip structure, the Baiyun-Liwan Fault Zone, in the northern South China Sea. This fault zone comprises NW- to NE-striking faults and negative flower structures that were generated by oblique extensional displacement. Notably, the interpreted data reveals that the Baiyun-Liwan Fault Zone was active during the Cenozoic, recording intense magmatism, and accommodating significant intraplate deformation during progressive continental rifting and ocean spreading. It bounds two distinct crustal segments and played a significant role in segmenting the northern margin of the South China Sea. The geometry of faults and strata within the Baiyun-Liwan Fault Zone also controlled local sediment routing and depocentre evolution during the Cenozoic. As basement and syn-rift structures change markedly across the Baiyun-Liwan Fault Zone, we propose this structure to be inherited from a lithospheric-scale fault zone separating the Mesozoic arc from forearc-related terrains. We therefore stress the importance of pre-existing structures in the development of rifted margins, with the example provided by the Baiyun-Liwan Fault Zone having profound implications for palaeogeographic reconstructions in the South China Sea. At present, the Baiyun-Liwan Fault Zone is incised by the Pearl River Canyon and eroded by recurrent submarine landslides, forming a major area of sediment bypass towards the abyssal plain. [ABSTRACT FROM AUTHOR]

Spinal cord injury (SCI) is a serious nervous system disease that usually leads to the impairment of the motor, sensory, and autonomic nervous functions of the spinal cord, and it places a heavy burden on families and healthcare systems every year. Due to the complex pathophysiological mechanism of SCI and the poor ability of neurons to regenerate, the current treatment scheme has very limited effects on the recovery of spinal cord function. In addition, due to their unique advantages, exosomes can be used as carriers for cargo transport. In recent years, some studies have confirmed that treatment with mesenchymal stem cells (MSCs) can promote the recovery of SCI nerve function. The therapeutic effect of MSCs is mainly related to exosomes secreted by MSCs, and exosomes may have great potential in SCI therapy. In this review, we summarized the repair mechanism of mesenchymal stem cells-derived exosomes (MSCs-Exos) in SCI treatment and discussed the microRNAs related to SCI treatment based on MSCs-Exos and their mechanism of action, which is helpful to further understand the role of exosomes in SCI.

Cancer stem cells (CSCs) are considered tumor-initiating cells and the main drivers of disease progression. Targeting these rare cancer cells, however, remains challenging with respect to therapeutic benefit. Here, we report the up-regulation of IL-13RA2 expression in colorectal cancer (CRC) tissues and spheroid cells. The expression of IL-13RA2 was positively correlated with canonical stemness markers in CRC. We further demonstrated that the level of IL-13 was up-regulated in the serum of CRC patients. Biologically, recombinant IL-13 (rIL-13) stimulation promoted the sphere formation, proliferation, and migration of CRC cells in vitro and enhanced tumorigenesis in vivo . This phenotype could be reversed by knocking down IL-13RA2. Mechanistically, IL-13 activated autophagy by inducing LC3I/LC3II transformation in CRC-CSCs, which was crucial for the biological functions of IL-13. We further demonstrated that IL-13RA2 acted as a modular link of the E3 ligase UBE3C and the substrate p53 protein, enhancing the interaction of UBE3C and p53, thereby inducing the K48-linked ubiquitination of p53. In conclusion, the IL-13/IL-13RA2 signaling cascade promotes CRC-CSC self-renewal and tumorigenesis by inducing p53 ubiquitination, adding an important layer to the connection between IL-13 and p53, which can be translated into novel targeted therapies., (© 2023 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.)

Wilms tumor gene (WT1) is used as a marker for the diagnosis and prognosis of ovarian cancer. However, the molecular mechanisms involving WT1 in ovarian cancer require further study. Herein, we used bioinformatics and other methods to identify important pathways and hub genes in ovarian cancer affected by WT1. The results showed that WT1 is highly expressed in ovarian cancer and is closely related to the overall survival and progression-free survival (PFS) of ovarian cancer. In ovarian cancer cell line SKOV3, WT1 downregulation increased the mRNA expression of 638 genes and decreased the mRNA expression of 512 genes, which were enriched in the FoxO, AMPK, and the Hippo signaling pathways. The STRING online tool and Cytoscape software were used to construct a Protein-protein interaction (PPI) network and for Module analysis, and 18 differentially expressed genes (DEGs) were selected. Kaplan-Meier plotter analysis revealed that 16 of 18 genes were related to prognosis. Analysis of GEPIA datasets indicated that 7 of 16 genes were differentially expressed in ovarian cancer tissues and in normal tissues. The expression of IGFBP1 and FBN1 genes increased significantly after WT1 interference, while the expression of the SERPINA1 gene decreased significantly. The correlation between WT1 expression and that of these three genes was consistent with that of ovarian cancer tissues and normal tissues. According to the GeneMANIA online website analysis, there were complex interactions between WT1, IGFBP1, FBN1, SERPINA1, and 20 other genes. In conclusion, we have identified important signaling pathways involving WT1 that affect ovarian cancer, and distinguished three differentially expressed genes regulated by WT1 associated with the prognosis of ovarian cancer. Our findings provide evidence outlining mechanisms involving WT1 gene expression in ovarian cancer and provides a rational for novel treatment of ovarian cancer., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Meng, Cao, Dong, Zhang, Ji and Wang.)

Ovarian cancer is one of the most common types of gynecological malignancy worldwide, and is the fourth leading cause of cancer-associated mortality among women. Despite improvements in therapeutic treatments, the prognosis for epithelial ovarian cancer (EOC) remains poor, mainly due to the rapid growth and metastasis of ovarian cancer tumors. An increasing number of studies have indicated that microRNAs (miRNAs) are involved in the carcinogenesis and progression of human cancer, suggesting that miRNAs may be used in clinical prognosis and as a therapeutic target in EOC. The aim of the present study was to investigate the expression levels of miRNA-494 in EOC tissues and cell lines. The clinical significance of miRNA-494 in patients with EOC was also evaluated. The results demonstrated that miRNA-494 was significantly downregulated in EOC tissues and cell lines. Low expression levels of miRNA-494 were associated with poor prognostic features, including International Federation of Gynecology and Obstetrics stage, tumor size and lymph node metastasis. In vitro functional studies demonstrated that overexpression of miRNA-494 inhibited proliferation, migration and invasion in EOC cells. By contrast, knockdown of miRNA-494 enhanced cell growth, migration and invasion in EOC cells. Notably, sirtuin 1 (SIRT1) was identified as a direct target of miRNA-494 in EOC. Furthermore, MTT, cell migration and invasion assays verified that EOC cell proliferation, migration and invasion were completely restored with forced miRNA-494 expression and SIRT1 restoration. Together, these findings suggest that miRNA-494 is a potential prognostic marker, and may provide novel therapeutic regimens of targeted therapy for EOC.

Background: We compared the T cell antigen receptor (TCR-BV) gene families of peripheral blood mononuclear cells (PMBC) between children with tuberculosis (TB) and those inoculated with the Bacille Calmette Guerin (BCG) vaccine., Methods: The total RNA was extracted from PMBC of 15 TB children, 15 BCG-vaccinated children and 15 healthy controls. The RNAs were reverse-transcribed and amplified by polymerase chain reaction (PCR). PCR products were separated on 1.5% agarose gel and analyzed with the Genescan technique., Results: Some TCR-BV gene families in TB children and BCG-vaccinated children exhibited a blur band in the predicted position on 1.5% agarose gel, some showed a distinct or fainted band. In general, many shared predominant clonal TCR-BV gene families (Vβ2, Vβ16, Vβ21, Vβ22) and the restricted-expression families (Vβ14 and Vβ17). All the gene families of the control children only exhibited blur bands and polyclonal., Conclusions: The skewed profile of TCR-BV gene families in TB children and BCG-vaccinated children are similar, which may probably explain the protective effects of BCG-vaccine against TB in children.