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Simple Summary: In this study, we performed microbial community ecology analyses of bacteria present in the spleen of six species of rodents in Panama in order to identify taxa with zoonotic potential in the country. Genera of bacteria containing species with zoonotic potential detected in this study included Acinetobacter, Bartonella, Cutibacterium, Enterococcus, and Staphylococcus. The results obtained are of value for estimating the prevalence and relative abundance of the bacteria found and the potential of different species of rodents as reservoirs of bacterial zoonosis. This study provides information for comparative studies in the Neotropics and other regions of the world and to generate knowledge on the conditions that may drive zoonosis in different rural vs. suburban environmental settings. Emerging zoonotic diseases are one of the main threats to human and animal health. Among the agents with the potential for zoonoses, those of bacterial origin have great relevance in Public Health. Rodents are considered one of the main reservoirs of pathogens that represent a risk to human health or animal species. We used massive 16S ribosomal RNA gene amplicon sequencing to survey bacteria present in the spleen of six species of rodents in Panama in order to identify bacterial taxa with zoonotic potential in the country. We found 3352 bacterial Amplicon Sequence Variants (ASVs, i.e., phylogenetic species) in the spleen of six rodent species surveyed (Liomys adspersus, Melanomys caliginosus, Mus musculus, Proechimys semispinosus, Rattus rattus, Zygodontomys brevicauda). This bacterial community was represented by 25 phyla, 55 classes, 140 orders, 268 families, and 508 genera. The three predominant phyla were Actinobacteria, Firmicutes, and Proteobacteria, and the five predominant classes were Actinobacteria, Alpha- and Gammaproteobacteria, Bacilli, and Clostridia. There were seven high-abundance genera: Acinetobacter, Bartonella, Cutibacterium, Enterococcus, Sarcina, Staphylococcus, and Wolbachia. Genera found with less abundance included Bradyrhizobium, Chryseobacterium, Clostridium, Corynebacterium, Lactobacillus, Pseudonocardia, Rhodococcus, and Sphingomonas. Some of these genera (high or low abundance) have clinical importance. The identification of bacterial taxa with zoonotic potential in rodent species performed here allows us to have surveillance mechanisms for these pathogens and to be able to recognize localities to be prioritized for prevention of transmission and outbreaks, thus being of value for public health in Panama. [ABSTRACT FROM AUTHOR]

Telmatometra withei (Bergroth, 1908) is a common water strider found across the Neotropics. Although, the taxonomy of T. withei is relatively well known, the specie's life cycle has not been characterized. We use field surveys and laboratory experiments to describe the life cycle of two populations of T. withei in the Pacific coast of Panama. Specifically, we compared the life cycle between fresh and brackish water populations known to show local adaptation to each environment. We also contrast the life cycle of T. withei with that of other water striders previously reported in the literature. We found that the average life cycle of T. withei is approximately 66.7 days, and this period did not vary significantly between fresh (65.0 days) and brackish (68.3 days) populations. However, these estimates were longer than those reported for other species of water strides. In addition, traits associated with reproductive success such as egg length and fecundity varied significantly between populations, with females from freshwater populations showing larger values. This suggests that salinity can have important consequences for reproductive traits in T. withei, even if the overall life cycle may not be affected by salinity. Thus, characterizing the life history of these Neotropical organisms is crucial to understand the rich freshwater biodiversity of this region, as well as its response to both natural and anthropogenic disturbances. [ABSTRACT FROM AUTHOR]

Mangrove ecosystems are threatened worldwide by a wide range of factors including climate change, coastal development, and pollution. The effects of these factors on soil bacterial communities of Neotropical mangroves and their temporal dynamics is largely undocumented. Here we compared the diversity and taxonomic composition of bacterial communities in the soil of two mangrove forest sites of the Panama Bay: Juan Diaz (JD), an urban mangrove forest in Panama City surrounded by urban development, with occurrence of five mangrove species, and polluted with solid waste and sewage; and Bayano (B), a rural mangrove forest without urban development, without solid waste pollution, and with the presence of two mangrove species. Massive amplicon sequencing of the V4 region of the 16S rRNA gene and community analyses were implemented. In total, 20,691 bacterial amplicon sequence variants were identified, and the bacterial community was more diverse in the rural mangrove forest based on Faith's phylogenetic diversity index. The three dominant phyla of bacteria found and shared between the two sites were Proteobacteria, Desulfobacterota, and Chloroflexi. The ammonia oxidizing archaea class Nitrosphaeria was found among the top 10 most abundant. Dominant genera of bacteria that occurred in the two mangrove sites were: BD2-11_terrestrial_group (Gemmatimonadota), EPR3968-O8a-Bc78 (Gammaproteobacteria), Salinimicrobium (Bacteroidetes), Sulfurovum (Campylobacteria), and Woeseia (Gammaproteobacteria) of which the first three and Methyloceanibacter had increased in relative abundance in the transition from rainy to dry to rainy season in the urban mangrove forest. Altogether, our study suggests that factors such as urban development, vegetation composition, pollution, and seasonal changes may cause shifts in bacterial diversity and relative abundance of specific taxa in mangrove soils. In particular, taxa with roles in biogeochemical cycles of carbon, nitrogen, sulfur, and phosphorus, and on rhizosphere taxa, could be important for mangrove plant resilience to environmental stress. [ABSTRACT FROM AUTHOR]

The evolution of local adaptation is crucial for the in situ persistence of populations in changing environments. However, selection along broad environmental gradients could render local adaptation difficult, and might even result in maladaptation. We address this issue by quantifying fitness trade‐offs (via common garden experiments) along a salinity gradient in two populations of the Neotropical water strider Telmatometra withei—a species found in both fresh (FW) and brackish (BW) water environments across Panama. We found evidence for local adaptation in the FW population in its home FW environment. However, the BW population showed only partial adaptation to the BW environment, with a high magnitude of maladaptation along naturally occurring salinity gradients. Indeed, its overall fitness was ~60% lower than that of the ancestral FW population in its home environment, highlighting the role of phenotypic plasticity, rather than local adaptation, in high salinity environments. This suggests that populations seemingly persisting in high salinity environments might in fact be maladapted, following drastic changes in salinity. Thus, variable selection imposed by salinization could result in evolutionary mismatch, where the fitness of a population is displaced from its optimal environment. Understanding the fitness consequences of persisting in fluctuating salinity environments is crucial to predict the persistence of populations facing increasing salinization. It will also help develop evolutionarily informed management strategies in the context of global change. [ABSTRACT FROM AUTHOR]

Salinization of freshwater ecosystems represents a potential threat to biodiversity, but the distribution of salinity tolerance among freshwater organisms and its functional consequences are understudied. In this study, we reviewed global patterns of salinity tolerance across a broad range of freshwater organisms. Specifically, we compared published data on LC (a metric of salinity tolerance) across climatic regions, taxa, and functional feeding groups (FFGs). We found that microinvertebrates were more sensitive to salinity than vertebrates and macroinvertebrates. Within aquatic insects, there was considerable variability in tolerance across FFGs. Specifically, scrapers, gatherers, and filterers were more sensitive on average than omnivores, shredders, and predators. Thus, we predict that increasing salinization can negatively impact trophic diversity and in turn cause overall changes in the structure and function of freshwater ecosystems. We also identified both historical exposure and taxonomic affinity as potential drivers of contemporary salinity tolerance across freshwater organisms. Finally, we found important gaps in our understanding of the potential impacts of salinization on freshwater biodiversity, particularly in regions expected to be affected by increased salinization due to climate change and secondary salinization. Understanding the differential vulnerability of freshwater taxa is critical to predicting the ecosystem impacts of salinization, and informing conservation and management decisions. [ABSTRACT FROM AUTHOR]

Simple Summary: Insects host a highly diverse bacterial community. Although we have a good understanding of the role that this microbiome plays in insects, the composition and diversity of microbiomes associated with Neotropical freshwater insects is virtually unknown. Here, we describe, for the first time, the microbiome associated with six species of Neotropical water striders in Panama. We also performed phylogenetic analyses to explore potential codiversification or coevolution between water strider species and their associated microbiome. We found a diverse microbiome associated with the six species of water striders, with the dominant bacterial taxa belonging to the phyla Proteobacteria and Tenericutes. Although some bacterial lineages were shared across species, some lineages were also uniquely associated with different water strider species. Our results suggest that both environmental variation and host phylogenetic identity are important drivers of the microbiome associated with water striders. Understanding the evolution of the host-microbiome interaction is crucial to our understanding of Neotropical freshwater ecosystems. Insects host a highly diverse microbiome, which plays a crucial role in insect life. However, the composition and diversity of microbiomes associated with Neotropical freshwater insects is virtually unknown. In addition, the extent to which diversification of this microbiome is associated with host phylogenetic divergence remains to be determined. Here, we present the first comprehensive analysis of bacterial communities associated with six closely related species of Neotropical water striders in Panama. We used comparative phylogenetic analyses to assess associations between dominant bacterial linages and phylogenetic divergence among species of water striders. We found a total of 806 16S rRNA amplicon sequence variants (ASVs), with dominant bacterial taxa belonging to the phyla Proteobacteria (76.87%) and Tenericutes (19.51%). Members of the α- (e.g., Wolbachia) and γ- (e.g., Acinetobacter, Serratia) Proteobacteria, and Mollicutes (e.g., Spiroplasma) were predominantly shared across species, suggesting the presence of a core microbiome in water striders. However, some bacterial lineages (e.g., Fructobacillus, Fluviicola and Chryseobacterium) were uniquely associated with different water strider species, likely representing a distinctive feature of each species' microbiome. These findings indicate that both host identity and environmental context are important drivers of microbiome diversity in water striders. In addition, they suggest that diversification of the microbiome is associated with diversification in water striders. Although more research is needed to establish the evolutionary consequences of host-microbiome interaction in water striders, our findings support recent work highlighting the role of bacterial community host-microbiome codiversification. [ABSTRACT FROM AUTHOR]