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Cardioselective β-adrenergic blocker metoprolol tartrate, is used in the treatment of different diseases such as cardiac arrhythmias, hypertension, heart failure, angina pectoris, migraine, and hyperthyroidism. Beside dosage forms of the parenteral ampoule and conventional tablets of different manufacturers, there are extended release tablets of metoprolol tartrate in the Turkish Drug Market. In this research work, comparative quality control studies of metoprolol tartrate extended release tablets (original and generic) produced by two different pharmaceutical companies in the Turkey were carried out and evaluated according to the related guidelines. Thickness and diameter, hardness, weight variation, friability, content uniformity and dissolution rate were examined as quality control parameters. A new validated HPLC method for the quantification of metoprolol tartrate has been developed. An ACE column (C18, 5 μm, 250x4.6 mm) and acetonitrile:phosphate buffer (30:70, v/v) mobile phase were used for the determination of metoprolol tartrate. The tablets showed extended release for 8 hours (70.74% release from drug A, 76.87% from the drug B). Both products have acceptable hardness, friability and weight variation values. Content of the active ingredient of the tablets was consistent with label claim (99.45% for drug A and 96.45% for drug B). The dissolution data were evaluated by model dependent and model independent methods using DDSolver program. The obtained results showed that release kinetics of both drugs were well fitted with the Korsmeyer-Peppas model. [ABSTRACT FROM AUTHOR]

The potential of capillary electrophoresis-mass spectrometry (CE-MS) for metabolomics is demonstrated through the analysis of metabolites from human HT29 colon cancer cells treated and non-treated with dietary polyphenols. Prior to CE-MS analysis, four different metabolite purification strategies are investigated. Namely, the results obtained after methanol deproteinization, ultrafiltration, and two solid-phase extraction methods using C18 and polymer-based cartridges are described. These generic methods can have broad applications to analyze metabolites in a large variety of matrices and fields, including the new Foodomics area.