36 results on '"Chen, Dewei"'
Search Results
2. Determination of Characteristic Taste Compounds of Crayfish by 1H Nuclear Magnetic Resonance
- Author
-
YAO Jingyu, LIU Jie, BAI Xueying, MO Xueying, CHEN Dewei
- Subjects
crayfish ,1h nuclear magnetic resonance ,taste-active compounds ,umami ,Food processing and manufacture ,TP368-456 - Abstract
In order to investigate the nonvolatile taste-active components in crayfish, free amino acids, nucleotides, organic acids, soluble sugars and alkaloids in cooked crayfish muscle were qualitatively and quantitatively analyzed by 1H nuclear magnetic resonance (1H NMR). Their taste activity values (TAVs) and equivalent umami concentration (EUC) were calculated to evaluate the key taste-active compounds and umami attributes, respectively. The results suggested that a total of 33 taste-active compounds were detected, among which arginine, histidine, glutamic acid, alanine, lysine, glycine, adenosine 5’-monophosphate (AMP), inosine 5’-mononucleotide (IMP), succinic acid and lactic acid were the key taste-active compounds with TAVs greater than 1.0, and they played an important role in the taste of crayfish. The EUC of crayfish was 9.1 g monosodium glutamate (MSG)/100 g, indicating very intense umami taste.
- Published
- 2023
- Full Text
- View/download PDF
3. Sinomenine alleviates lipopolysaccharide-induced acute lung injury via a PPARβ/δ-dependent mechanism
- Author
-
Zhao, Li, Zhang, Mengjie, Liu, Yang-Wuyue, Tan, Yan, Yin, Jun, Chen, Yuanyuan, Chen, Dewei, and Ni, Bing
- Published
- 2023
- Full Text
- View/download PDF
4. Stanniocalcin-1 Protected Astrocytes from Hypoxic Damage Through the AMPK Pathway
- Author
-
Sun, Binda, He, Shu, Liu, Bao, Xu, Gang, Guoji E, Feng, Lan, Xu, Licong, Chen, Dewei, Zhao, Wenqi, Chen, Jian, Gao, Yuqi, and Zhang, Erlong
- Published
- 2021
- Full Text
- View/download PDF
5. Green synthesis of 1,4-dihydropyridines using cobalt carbon nanotubes as recyclable catalysts
- Author
-
Wu, Kaier, Bai, Yuye, Chen, Dewei, Chen, Lu, Huang, Yubing, Bai, Shuli, and Li, Yibiao
- Published
- 2021
- Full Text
- View/download PDF
6. Review of design and innovative construction of steel truss cable-stayed bridges in China.
- Author
-
Yu, Xiangmin, Chen, Dewei, and Yu, Jinhong
- Subjects
- *
CABLE-stayed bridges , *BRIDGE design & construction , *SUSPENSION bridges , *STEEL - Abstract
Many steel truss cable-stayed bridges have been built in China in the past two decades, providing a wealth of design and construction experience. This paper provides a review of their development, the structural configurations used, the mechanical characteristics of decks, pylons and cables, and the various innovative construction methods adopted. With main spans now exceeding 1 km, the sturdy structures are mainly used for combined rail-and-road crossings in preference to more flexible suspension bridges. The aim of the paper is to provide a comprehensive and useful reference for further design and construction of steel truss cable-stayed bridges worldwide. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
7. Critical effects of epigenetic regulation in pulmonary arterial hypertension
- Author
-
Chen, Dewei, Gao, Wenxiang, Wang, Shouxian, Ni, Bing, and Gao, Yuqi
- Published
- 2017
- Full Text
- View/download PDF
8. Endothelial MRTF-A mediates angiotensin II induced cardiac hypertrophy
- Author
-
Weng, Xinyu, Yu, Liming, Liang, Peng, Chen, Dewei, Cheng, Xian, Yang, Yuyu, Li, Luyang, Zhang, Ting, Zhou, Bisheng, Wu, Xiaoyan, Xu, Huihui, Fang, Mingming, Gao, Yuqi, Chen, Qi, and Xu, Yong
- Published
- 2015
- Full Text
- View/download PDF
9. In silico modeling of functionalized graphene oxide-metal cluster conjugates as Raman probe: Raman activity of pyridine
- Author
-
Chen, Dewei, Copeland, Christopher, Majumdar, D., Roszak, Szczepan, and Leszczynski, Jerzy
- Published
- 2017
- Full Text
- View/download PDF
10. Chemerin Regulates the Proliferation and Migration of Pulmonary Arterial Smooth Muscle Cells via the ERK1/2 Signaling Pathway.
- Author
-
Peng, Linqian, Chen, Yunwei, Li, Yan, Feng, Panpan, Zheng, Yan, Dong, Yongjie, Yang, Yunjing, Wang, Ruiyu, Li, Ailing, Yan, Jianghong, Shang, Feifei, Tang, Ping, Chen, Dewei, Gao, Yuqi, and Huang, Wei
- Subjects
CHEMERIN ,SMOOTH muscle ,MUSCLE cells ,PULMONARY arterial hypertension ,CELLULAR signal transduction ,RECEIVER operating characteristic curves - Abstract
Pulmonary arterial hypertension (PAH) is an incurable disease with high mortality. Chemerin has been found to be associated with pulmonary hypertension (PH). However, the specific role of chemerin in mediating PH development remains unclear. This study aimed to elucidate the regulatory effects and the underlying mechanism of chemerin on PH and to investigate the expression levels of chemerin protein in plasma in PAH patients. In vivo , two animal models of PH were established in rats by monocrotaline (MCT) injection and hypoxia. We found that the expression levels of chemerin and its receptor, chemokine-like receptor 1 (CMKLR1), were significantly upregulated in the lungs of PH rats. Primary cultured pulmonary arterial smooth muscle cells [(PASMCs) (isolated from pulmonary arteries of normal healthy rats)] were exposed to hypoxia or treated with recombinant human chemerin, we found that CMKLR1 expression was upregulated in PASMCs in response to hypoxia or chemerin stimulation, whereas the exogenous chemerin significantly promoted the migration and proliferation of PASMCs. Notably, the regulatory effects of chemerin on PASMCs were blunted by PD98059 (a selective ERK1/2 inhibitor). Using enzyme linked immunosorbent assay (ELISA), we found that the protein level of chemerin was also markedly increased in plasma from idiopathic pulmonary arterial hypertension (IPAH) patients compared to that from healthy controls. Moreover, the diagnostic value of chemerin expression in IPAH patients was determined through receiver operating characteristic (ROC) curve analysis and the result revealed that area under ROC curve (AUC) for plasma chemerin was 0.949. Taken together, these results suggest that chemerin exacerbates PH progression by promoting the proliferation and migration of PASMCs via the ERK1/2 signaling pathway, and chemerin is associated with pulmonary hypertension. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
11. Brahma-related gene 1 (Brg1) epigenetically regulates CAM activation during hypoxic pulmonary hypertension
- Author
-
Chen, Dewei, Fang, Fei, Yang, Yuyu, Chen, Jian, Xu, Gang, Xu, Yong, and Gao, Yuqi
- Published
- 2013
- Full Text
- View/download PDF
12. A Data-Center FPGA Acceleration Platform for Convolutional Neural Networks
- Author
-
Chen Dewei, Zhang Bo, Ephrem C. Wu, Jie Miao, Wang Yuwei, Meng Yu, Zhang Heng, Yu Xiaoyu, Biao Min, and Gao Jianlin
- Subjects
FOS: Computer and information sciences ,Computer Science - Machine Learning ,Computer science ,Computer Vision and Pattern Recognition (cs.CV) ,Computer Science - Computer Vision and Pattern Recognition ,Cloud computing ,02 engineering and technology ,01 natural sciences ,Convolutional neural network ,Computational science ,Machine Learning (cs.LG) ,Acceleration ,Gate array ,0103 physical sciences ,Hardware Architecture (cs.AR) ,0202 electrical engineering, electronic engineering, information engineering ,Field-programmable gate array ,Computer Science - Hardware Architecture ,Throughput (business) ,010302 applied physics ,business.industry ,Deep learning ,020202 computer hardware & architecture ,Filter (video) ,Artificial intelligence ,business - Abstract
Intensive computation is entering data centers with multiple workloads of deep learning. To balance the compute efficiency, performance, and total cost of ownership (TCO), the use of a field-programmable gate array (FPGA) with reconfigurable logic provides an acceptable acceleration capacity and is compatible with diverse computation-sensitive tasks in the cloud. In this paper, we develop an FPGA acceleration platform that leverages a unified framework architecture for general-purpose convolutional neural network (CNN) inference acceleration at a data center. To overcome the computation bound, 4,096 DSPs are assembled and shaped as supertile units (SUs) for different types of convolution, which provide up to 4.2 TOP/s 16-bit fixed-point performance at 500 MHz. The interleaved-task-dispatching method is proposed to map the computation across the SUs, and the memory bound is solved by a dispatching-assembling buffering model and broadcast caches. For various non-convolution operators, a filter processing unit is designed for general-purpose filter-like/pointwise operators. In the experiment, the performances of CNN models running on server-class CPUs, a GPU, and an FPGA are compared. The results show that our design achieves the best FPGA peak performance and a throughput at the same level as that of the state-of-the-art GPU in data centers, with more than 50 times lower latency.
- Published
- 2019
13. Megakaryocytic leukemia 1 (MKL1) ties the epigenetic machinery to hypoxia-induced transactivation of endothelin-1
- Author
-
Yang, Yuyu, Chen, Dewei, Yuan, Zhibin, Fang, Fei, Cheng, Xian, Xia, Jun, Fang, Mingming, Xu, Yong, and Gao, Yuqi
- Published
- 2013
- Full Text
- View/download PDF
14. Effects of low temperature soaking on color and texture of green eggplants
- Author
-
Zhang, Min and Chen, Dewei
- Published
- 2006
- Full Text
- View/download PDF
15. Comparative Research of Extra-large-span Cable-stayed Bridge with Steel Truss Girder and Steel Box Girder
- Author
-
Tan Manjiang, Bai Zhizhou, and Chen Dewei
- Subjects
steel truss girder ,steel box girder ,large-span cable-stayed bridge ,comparative analysis ,Engineering (General). Civil engineering (General) ,TA1-2040 - Abstract
To research structural performance of extra-large-span cable-stayed bridge under different section forms, with the engineering background of a 800m main-span cable-stayed bridge with steel truss girder, the cable-stayed bridge with steel box girder is designed according to the current bridge regulations when two bridges are designed in an ultimate state of the carrying capacity, so the maximum stress and minimum stress of the stress envelope diagram are substantially the same. A comprehensive comparison is given to two types of bridge on the aspect of static force, natural vibration frequency, stability, economic performance and so on. Analysis results provide future reference for the large-span cable-stayed bridge to select between the steel truss girder and the steel box girder.
- Published
- 2015
- Full Text
- View/download PDF
16. SIRT1 links CIITA deacetylation to MHC II activation
- Author
-
Wu, Xiaoyan, Kong, Xiaocen, Chen, Dewei, Li, He, Zhao, Yuhao, Xia, Minjie, Fang, Mingming, Li, Ping, Fang, Fei, Sun, Lina, Tian, Wenfang, Xu, Huihui, Yang, Yuyu, Qi, Xiaohong, Gao, Yuqi, Sha, Jiahao, Chen, Qi, and Xu, Yong
- Published
- 2011
- Full Text
- View/download PDF
17. Increased Frequency of Circulating Classical Monocytes in Patients with Rosacea.
- Author
-
Gao, Cuie, Ge, Lan, Chen, Dewei, Zhang, Mengjie, Zhao, Li, Liu, Wenying, Chen, Shuguang, Wang, Juan, Zhou, Cunjian, Zhao, Xingwang, Li, Shifei, Song, Zhiqiang, and Li, Jian
- Subjects
MONONUCLEAR leukocytes ,MONOCYTES ,ROSACEA ,TUMOR necrosis factors ,SYSTEMIC lupus erythematosus - Abstract
Purpose: Monocyte subsets, including classical, intermediate and non-classical monocytes, are involved in the pathogenesis of inflammatory or autoimmune diseases. The pathogenic role of monocytes in the peripheral blood mononuclear cells (PBMCs) of patients with rosacea remains unclear. This study aimed to assess frequencies of monocyte subsets in PBMCs from rosacea patients before and after clinical treatment. Patients and Methods: We applied flow cytometry to examine frequencies of monocyte subsets in 116 patients with rosacea, while patients with 26 systemic lupus erythematosus (SLE), 28 acne and 42 normal healthy subjects without skin problems (HC) were recruited as controls. Expression of C–C chemokine receptor 2 (CCR2) on monocytes and plasma levels of CC-chemokine ligand 2 (CCL2), high mobility group box-1 (HMGB-1), interleukin-1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) were measured in HC and rosacea patients before and after treatment. Results: The frequency of classical monocytes, but not intermediate or non-classical monocytes, was higher in rosacea as compared with HC, which decreased after treatment. Frequencies of monocyte subsets showed no gender difference, while increased with age in patients but not in HC. Frequencies of classical monocytes in patients with erythematotelangiectatic rosacea (ETR) and ETR-papulopustular rosacea (PPR) overlap were significantly higher than HC or patients with only PPR or phymatous rosacea (PhR). There was a significant higher expression of CCR2 in classical monocytes, with higher plasma levels of CCL2, HMGB-1, IL-1β and TNF-α in patients than in HC, which all significantly decreased after treatment. Conclusion: Our data indicated a possible association between abnormal classical monocytes frequencies and rosacea. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
18. Nogo-B Receptor Directs Mitochondria-Associated Membranes to Regulate Vascular Smooth Muscle Cell Proliferation
- Author
-
Lan Feng, Yuqi Gao, Xu Gang, Zhang Erlong, Chen Dewei, Yidong Yang, Chen Jian, and Man-Man Li
- Subjects
0301 basic medicine ,Male ,Vascular smooth muscle ,030204 cardiovascular system & hematology ,Mitochondrion ,Endoplasmic Reticulum ,Muscle, Smooth, Vascular ,lcsh:Chemistry ,0302 clinical medicine ,Inositol 1,4,5-Trisphosphate Receptors ,Phosphorylation ,Receptor ,Hypoxia ,lcsh:QH301-705.5 ,Spectroscopy ,Chemistry ,General Medicine ,Computer Science Applications ,Cell biology ,MAM ,Mitochondrial Membranes ,medicine.symptom ,Signal Transduction ,Hypertension, Pulmonary ,Nogo Proteins ,NgBR ,Myocytes, Smooth Muscle ,VSMCs ,Down-Regulation ,Pulmonary Artery ,Models, Biological ,Catalysis ,Article ,Cell Line ,Inorganic Chemistry ,03 medical and health sciences ,Downregulation and upregulation ,medicine ,Animals ,Physical and Theoretical Chemistry ,Molecular Biology ,Cell Proliferation ,Cell growth ,Endoplasmic reticulum ,Organic Chemistry ,Hypoxia (medical) ,Rats ,030104 developmental biology ,lcsh:Biology (General) ,lcsh:QD1-999 ,Calcium ,Proto-Oncogene Proteins c-akt - Abstract
Mitochondria-associated membranes (MAM) are a well-recognized contact link between the mitochondria and endoplasmic reticulum that affects mitochondrial biology and vascular smooth muscle cells (VSMCs) proliferation via the regulation of mitochondrial Ca2+(Ca2+m) influx. Nogo-B receptor (NgBR) plays a vital role in proliferation, epithelial-mesenchymal transition, and chemoresistance of some tumors. Recent studies have revealed that downregulation of NgBR, which stimulates the proliferation of VSMCs, but the underlying mechanism remains unclear. Here, we investigated the role of NgBR in MAM and VSMC proliferation. We analyzed the expression of NgBR in pulmonary arteries using a rat model of hypoxic pulmonary hypertension (HPH), in which rats were subjected to normoxic recovery after hypoxia. VSMCs exposed to hypoxia and renormoxia were used to assess the alterations in NgBR expression in vitro. The effect of NgBR downregulation and overexpression on VSMC proliferation was explored. The results revealed that NgBR expression was negatively related with VSMCs proliferation. Then, MAM formation and the phosphorylation of inositol 1,4,5-trisphosphate receptor type 3 (IP3R3) was detected. We found that knockdown of NgBR resulted in MAM disruption and augmented the phosphorylation of IP3R3 through pAkt, accompanied by mitochondrial dysfunction including decreased Ca2+m, respiration and mitochondrial superoxide, increased mitochondrial membrane potential and HIF-1&alpha, nuclear localization, which were determined by confocal microscopy and Seahorse XF-96 analyzer. By contrast, NgBR overexpression attenuated IP3R3 phosphorylation and HIF-1&alpha, nuclear localization under hypoxia. These results reveal that dysregulation of NgBR promotes VSMC proliferation via MAM disruption and increased IP3R3 phosphorylation, which contribute to the decrease of Ca2+m and mitochondrial impairment.
- Published
- 2019
19. Design and construction of the Tahya Misr cable-stayed bridge in Cairo, Egypt.
- Author
-
Yu, Xiangmin and Chen, Dewei
- Subjects
- *
CABLE-stayed bridges , *BRIDGE maintenance & repair , *COMPOSITE structures , *TRAFFIC lanes - Abstract
The 540 m Tahya Misr Bridge over the Nile in Cairo, Egypt was completed in 2019. At 67.3 m wide, it is the widest cable-stayed crossing in the world. The crossing has an innovative configuration, with separate decks for each carriageway carried by merged pylons that share their inner legs. This paper highlights the unique design features and special construction considerations of the shared-pylon design. The geometry and site control strategies are explained along with the bridge closure process, which was based on the natural temperature method. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
20. Yachihe Bridge, China: engineering the world's longest cable-stayed steel truss.
- Author
-
Yu, Xiangmin, Chen, Dewei, and Xue, Menggui
- Subjects
- *
CABLE-stayed bridges , *ANCHORAGE (Structural engineering) , *TECHNOLOGICAL innovations ,QIANXINAN (Guizhou, China) - Abstract
Yachihe Bridge in China is the longest steel-truss, cable-stayed bridge in the world and the tenth longest overall. Completed in 2016, its 800 m main span carries the new Guiyang-Qianxi dual carriageway over the Yachihe River gorge. It is also the first cable-stayed bridge to be erected using a cable crane, and its concrete-box-girder side spans feature the first use of cable anchorages in the middle of the outer web. Furthermore, geometry alignment during deck closure was achieved by adjusting cable forces rather than by using counterweights. This paper describes the configuration and numerical analysis of the innovative cable anchorage, discusses the challenges and solutions involved in using a cable crane for construction and details the novel closure techniques adopted for the steel-truss deck. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
21. Innovative Method for the Construction of Cable-Stayed Bridges by Cable Crane.
- Author
-
Yu, Xiangmin and Chen, Dewei
- Subjects
CABLE-stayed bridge design & construction ,TECHNOLOGICAL innovations ,CRANES (Machinery) ,MECHANICAL loads ,BRIDGES - Abstract
Yachihe Bridge in China is a hybrid cable-stayed bridge with concrete decks at the side spans and a steel truss deck at the mid-span. The 800-m-long truss deck breaks a new record as the longest steel truss cable-stayed bridge in the world. Constrained by site terrain, poor transportation conditions and limited time, a cable crane was adopted for the erection of the 16-m-long truss deck segments, marking the first time that a cable crane has been used in the deck erection of a cable-stayed bridge. This paper primarily deals with the challenges faced and solutions formulated during the bridge construction process, and also presents field load tests for the cable crane. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
22. Pro-Inflammatory Stimuli Engage Brahma Related Gene 1 (Brg1) and Brahma (Brm) in Endothelial Injury
- Author
-
Fang, Fei, Chen, Dewei, Yu, Limin, Dai, Xin, Yang, Yuyu, Tian, Wenfang, Cheng, Xian, Xu, Huihui, Fang, Mingming, Zhou, Jiliang, Gao, Yuqi, Chen, Qi, and Xu, Yong
- Subjects
Transcriptional Activation ,Time Factors ,Transfection ,Article ,Epigenesis, Genetic ,Mice ,Apolipoproteins E ,Cell Line, Tumor ,Human Umbilical Vein Endothelial Cells ,Animals ,Humans ,Promoter Regions, Genetic ,Mice, Knockout ,Binding Sites ,Estradiol ,DNA Helicases ,NF-kappa B ,Endothelial Cells ,Nuclear Proteins ,Vascular System Injuries ,Disease Models, Animal ,HEK293 Cells ,RNA Interference ,Endothelium, Vascular ,Inflammation Mediators ,Cell Adhesion Molecules ,Signal Transduction ,Transcription Factors - Abstract
Endothelial dysfunction inflicted by inflammation is found in a host of cardiovascular pathologies. One hallmark event in this process is the aggregation and adhesion of leukocyte to the vessel wall mediated by the upregulation of adhesion molecules (CAM) in endothelial cells at the transcriptional level. The epigenetic modulator(s) of CAM transactivation and its underlying pathophysiological relevance remain poorly defined.Our goal was to determine the involvement of Brahma related gene 1 (Brg1) and Brahma (Brm) in CAM transactivation and its relevance in the pathogenesis of atherosclerosis.In the present study, we report that proinflammatory stimuli augmented the expression of Brg1 and Brm in vitro in cultured endothelial cells and in vivo in arteries isolated from rodents. Overexpression of Brg1 and Brm promoted while knockdown of Brg1 and Brm abrogated transactivation of adhesion molecules and leukocyte adhesion induced by inflammatory signals. Brg1 and Brm interacted with and were recruited to the CAM promoters by nuclear factor κB/p65. Conversely, depletion of Brg1 and Brm disrupted the kinetics of p65 binding on CAM promoters and crippled CAM activation. Silencing of Brg1 and Brm also altered key epigenetic changes associated with CAM transactivation. Of intrigue, 17β-estradiol antagonized both the expression and activity of Brg1/Brm. Most importantly, endothelial-targeted elimination of Brg1/Brm conferred atheroprotective effects to Apoe(-/-) mice on a Western diet.Our data suggest that Brg1 and Brm integrate various proinflammatory cues into CAM transactivation and endothelial malfunction and, as such, may serve as potential therapeutic targets in treating inflammation-related cardiovascular diseases.
- Published
- 2013
23. Virtual Prototype Design Technique of the CJ20-100 Model Alternating Current Contactor.
- Author
-
Chen, Dewei, Zhuang, Yuqi, Xie, Yangxing, and Huang, Yuanpeng
- Published
- 2014
- Full Text
- View/download PDF
24. Query-Focused Summarization by Combining Topic Model and Affinity Propagation.
- Author
-
Chen, Dewei, Tang, Jie, Yao, Limin, Li, Juanzi, and Zhou, Lizhu
- Abstract
The goal of query-focused summarization is to extract a summary for a given query from the document collection. Although much work has been done for this problem, there are still many challenging issues: (1) The length of the summary is predefined by, for example, the number of word tokens or the number of sentences. (2) A query usually asks for information of several perspectives (topics); however existing methods cannot capture topical aspects with respect to the query. In this paper, we propose a novel approach by combining statistical topic model and affinity propagation. Specifically, the topic model, called qLDA, can simultaneously model documents and the query. Moreover, the affinity propagation can automatically discover key sentences from the document collection without predefining the length of the summary. Experimental results on DUC05 and DUC06 data sets show that our approach is effective and the summarization performance is better than baseline methods. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
25. SEWSIP: semantic based Web services integration in P2P.
- Author
-
Li Juanzi, Xu Bin, Yang Wenjun, Chen Dewei, Zhang Po, and Wang Kehong
- Published
- 2005
- Full Text
- View/download PDF
26. Megakaryocytic Leukemia 1 (MKL1) Regulates Hypoxia Induced Pulmonary Hypertension in Rats.
- Author
-
Yuan, Zhibin, Chen, Jian, Chen, Dewei, Xu, Gang, Xia, Minjie, Xu, Yong, and Gao, Yuqi
- Subjects
LEUKEMIA ,HYPOXEMIA ,PULMONARY hypertension ,LABORATORY rats ,HAIRPIN (Genetics) ,CHEMOKINES ,MUSCLE cells - Abstract
Hypoxia induced pulmonary hypertension (HPH) represents a complex pathology that involves active vascular remodeling, loss of vascular tone, enhanced pulmonary inflammation, and increased deposition of extracellular matrix proteins. Megakaryocytic leukemia 1 (MKL1) is a transcriptional regulator known to influence cellular response to stress signals in the vasculature. We report here that in response to chronic hypobaric hypoxia, MKL1 expression was up-regulated in the lungs in rats. Short hairpin RNA (shRNA) mediated depletion of MKL1 significantly ameliorated the elevation of pulmonary arterial pressure in vivo with a marked alleviation of vascular remodeling. MKL1 silencing also restored the expression of NO, a key vasoactive molecule necessary for the maintenance of vascular tone. In addition, hypoxia induced pulmonary inflammation was dampened in the absence of MKL1 as evidenced by normalized levels of pro-inflammatory cytokines and chemokines as well as reduced infiltration of pro-inflammatory immune cells in the lungs. Of note, MKL1 knockdown attenuated fibrogenesis in the lungs as indicated by picrosirius red staining. Finally, we demonstrate that MKL1 mediated transcriptional activation of type I collagen genes in smooth muscle cells under hypoxic conditions. In conclusion, we data highlight a previously unidentified role for MKL1 in the pathogenesis of HPH and as such lay down groundwork for future investigation and drug development. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
27. Proinflammatory Stimuli Engage Brahma Related Gene 1 and Brahma in Endothelial Injury.
- Author
-
Fang, Fei, Chen, Dewei, Yu, Liming, Dai, Xin, Yang, Yuyu, Tian, Wenfang, Cheng, Xian, Xu, Huihui, Weng, Xinyu, Fang, Mingming, Zhou, Jiliang, Gao, Yuqi, Chen, Qi, and Xu, Yong
- Published
- 2013
- Full Text
- View/download PDF
28. Research on the architecture and modeling method of a fixture intelligent computer-aided design system.
- Author
-
Chen, Dewei, Cheng, Yaodong, Pang, Xiaohong, Chen, Ting, and Li, Chunguang
- Published
- 1995
- Full Text
- View/download PDF
29. Discovering the staring people from social networks.
- Author
-
Chen, Dewei, Tang, Jie, Li, Juanzi, and Zhou, Lizhu
- Published
- 2009
- Full Text
- View/download PDF
30. Corrigendum to "Interaction between plant phenolics and rice protein improved oxidative stabilities of emulsion" [J. Cereal Sci. 89 (2019) 102818].
- Author
-
Jia, Xiao, Zhao, Mouming, Xia, Ning, Teng, Jianwen, Jia, Chunxiao, Wei, Baoyao, Huang, Li, and Chen, Dewei
- Subjects
- *
RICE proteins , *PHENOLS , *EMULSIONS , *MOLECULAR structure , *FOOD chemistry - Published
- 2020
- Full Text
- View/download PDF
31. Interaction between plant phenolics and rice protein improved oxidative stabilities of emulsion.
- Author
-
Jia, Xiao, Zhao, Mouming, Xia, Ning, Teng, Jianwen, Jia, Chunxiao, Wei, Baoyao, Huang, Li, and Chen, Dewei
- Subjects
- *
RICE proteins , *FERULIC acid , *HYDROPEROXIDES , *HYDROPHOBIC interactions , *CORN oil , *EMULSIONS , *PHENOLS - Abstract
The interaction between rice protein isolate (RPI) and ferulic acid (FA) was systematically investigated. The results showed that fluorescence intensity of the RPI decreased gradually upon increasing the concentration of ferulic acid, and the maximum emission shifted from 352.0 to 359.2 nm. It was proposed that a static quenching of RPI-FA complex occurred, due to the hydrophobic interactions. Moreover, CD spectra and FT-IR spectroscopy data suggested that the concentration of β-turn and α-helix decreased while those of random coil and β-sheet increased in RPI-FA complex. The decrease of intensity of the amide I band and amide II band in the RPI-FA complex implied a significant reduction of protein α-helical structure and the presence of non-polar hydrophobic interactions. In addition, SDS-PAGE results demonstrated that ferulic acid reacted with glutelin acidic subunits (34–37 KDa) as well as globulin (26 KDa), and ferulic acid might bind with aromatic amino acid residues of RPI. Furthermore, the RPI-FA complex exhibited high DPPH• scavenging ability, ABTS+• scavenging ability and ORAC value. Finally, emulsion stabilized by RPI-FA complex could decrease the concentration of hydroperoxide, TBARS, and hexanal, thereby effectively restraining fat oxidation degradation. Image 1 • Rice protein isolate and ferulic acid were interacted by hydrophobic interactions. • Ferulic acid reacted with gluten acid subunits and globulin of RPI. • RPI-FA complex could improve the oxidative stability of corn oil in emulsion. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
32. Nitric Oxide Ameliorates the Effects of Hypoxia in Mice by Regulating Oxygen Transport by Hemoglobin.
- Author
-
Zhou X, Su W, Bao Q, Cui Y, Li X, Yang Y, Yang C, Wang C, Jiao L, Chen D, and Huang J
- Abstract
Xiaoying Zhou, Wenting Su, Quanwei Bao, Yu Cui, Xiaoxu Li, Yidong Yang, Chengzhong Yang, Chengyuan Wang, Li Jiao, Dewei Chen, and Jian Huang. Nitric oxide ameliorates the effects of hypoxia in mice by regulating oxygen transport by hemoglobin. High Alt Med Biol . 00:00-00, 2024.-Hypoxia is a common pathological and physiological phenomenon in ischemia, cancer, and strenuous exercise. Nitric oxide (NO) acts as an endothelium-derived relaxing factor in hypoxic vasodilation and serves as an allosteric regulator of hemoglobin (Hb). However, the ultimate effects of NO on the hematological system in vivo remain unknown, especially in extreme environmental hypoxia. Whether NO regulation of the structure of Hb improves oxygen transport remains unclear. Hence, we examined whether NO altered the oxygen affinity of Hb (Hb-O
2 affinity) to protect extremely hypoxic mice. Mice were exposed to severe hypoxia with various concentrations of NO, and the survival time, exercise capacity, and other physical indexes were recorded. The survival time was prolonged in the 5 ppm NO (6.09 ± 1.29 minutes) and 10 ppm NO (6.39 ± 1.58 minutes) groups compared with the 0 ppm group (4.98 ± 1.23 minutes). Hypoxia of the brain was relieved, and the exercise exhaustion time was prolonged when mice inhaled 20 ppm NO (24.70 ± 6.87 minutes vs. 20.23 ± 6.51 minutes). In addition, the differences in arterial oxygen saturation (SO2 %) (49.64 ± 7.29% vs. 42.90 ± 4.30%) and arteriovenous SO2 % difference (25.14 ± 8.95% vs. 18.10 ± 6.90%) obviously increased. In ex vivo experiments, the oxygen equilibrium curve (OEC) left shifted as P50 decreased from 43.77 ± 2.49 mmHg (0 ppm NO) to 40.97 ± 1.40 mmHg (100 ppm NO) and 38.36 ± 2.78 mmHg (200 ppm NO). Furthermore, the Bohr effect of Hb was enhanced by the introduction of 200 ppm NO (-0.72 ± 0.062 vs.-0.65 ± 0.051), possibly allowing Hb to more easily offload oxygen in tissue at lower pH. The crystal structure reveals a greater distance between Asp94β-His146β in nitrosyl -Hb(NO-Hb), NO-HbβCSO93, and S-NitrosoHb(SNO-Hb) compared to tense Hb(T-Hb, 3.7 Å, 4.3 Å, and 5.8 Å respectively, versus 3.5 Å for T-Hb). Moreover, hydrogen bonds were less likely to form, representing a key limitation of relaxed Hb (R-Hb). Upon NO interaction with Hb, hydrogen bonds and salt bridges were less favored, facilitating relaxation. We speculated that NO ameliorated the effects of hypoxia in mice by promoting erythrocyte oxygen loading in the lung and offloading in tissues.- Published
- 2024
- Full Text
- View/download PDF
33. Hypoxia-Induced Myocardial Hypertrophy Companies with Apoptosis Enhancement and p38-MAPK Pathway Activation.
- Author
-
Li X, Pu Z, Xu G, Yang Y, Cui Y, Zhou X, Wang C, Zhong Z, Zhou S, Yin J, Shan F, Yang C, Jiao L, Chen D, and Huang J
- Abstract
Li, Xiaoxu, Zhijun Pu, Gang Xu, Yidong Yang, Yu Cui, Xiaoying Zhou, Chenyuan Wang, Zhifeng Zhong, Simin Zhou, Jun Yin, Fabo Shan, Chengzhong Yang, Li Jiao, Dewei Chen, and Jian Huang. Hypoxia-induced myocardial hypertrophy companies with apoptosis enhancement and p38-MAPK pathway activation. High Alt Med Biol. 00:00-00, 2024. Background: Right ventricular function and remodeling are closely associated with symptom severity and patient survival in hypoxic pulmonary hypertension. However, the detailed molecular mechanisms underlying hypoxia-induced myocardial hypertrophy remain unclear. Methods: In Sprague-Dawley rats, hemodynamics were assessed under both normoxia and hypobaric hypoxia at intervals of 7 (H7), 14 (H14), and 28 (H28) days. Morphological changes in myocardial tissue were examined using hematoxylin and eosin (HE) staining, while myocardial hypertrophy was evaluated with wheat germ agglutinin (WGA) staining. Apoptosis was determined through TUNEL assays. To further understand the mechanism of myocardial hypertrophy, RNA sequencing was conducted, with findings validated via Western blot analysis. Results: The study demonstrated increased hypoxic pulmonary hypertension and improved right ventricular diastolic and systolic function in the rat models. Significant elevations in pulmonary arterial systolic pressure (PASP), mean pulmonary arterial pressure (mPAP), right ventricular mean pressure (RVMP), and the absolute value of +dp/dt
max were observed in the H14 and H28 groups compared with controls. In addition, right ventricular systolic pressure (RVSP), -dp/dtmax , and the mean dp/dt during isovolumetric relaxation period were notably higher in the H28 group. Heart rate increased in the H14 group, whereas the time constant of right ventricular isovolumic relaxation (tau) was reduced in both H14 and H28 groups. Both the right heart hypertrophy index and the heart weight/body weight ratio (HW/BW) were elevated in the H14 and H28 groups. Myocardial cell cross-sectional area also increased, as shown by HE and WGA staining. Western blot results revealed upregulated HIF-1α levels and enhanced HIF-2α expression in the H7 group. In addition, phosphorylation of p38 and c-fos was augmented in the H28 group. The H28 group showed elevated levels of Cytochrome C (Cyto C), whereas the H14 and H28 groups exhibited increased levels of Cleaved Caspase-3 and the Bax/Bcl-2 ratio. TUNEL analysis revealed a rise in apoptosis with the extension of hypoxia duration in the right ventricle. Conclusions: The study established a link between apoptosis and p38-MAPK pathway activation in hypoxia-induced myocardial hypertrophy, suggesting their significant roles in this pathological process.- Published
- 2024
- Full Text
- View/download PDF
34. EPAS1 (Endothelial PAS Domain Protein 1) Orchestrates Transactivation of Endothelial ICAM1 (Intercellular Adhesion Molecule 1) by Small Nucleolar RNA Host Gene 5 (SNHG5) to Promote Hypoxic Pulmonary Hypertension.
- Author
-
Wang S, Wang Y, Liu C, Xu G, Gao W, Hao J, Zhang M, Wu G, Yang Y, Huang J, Ni B, Chen D, and Gao Y
- Subjects
- Animals, Humans, Indans pharmacology, Male, Mice, Mice, Inbred C57BL, MicroRNAs physiology, Sulfones pharmacology, Basic Helix-Loop-Helix Transcription Factors physiology, Hypertension, Pulmonary etiology, Hypoxia complications, Intercellular Adhesion Molecule-1 genetics, RNA, Small Nucleolar physiology, Transcriptional Activation
- Abstract
[Figure: see text].
- Published
- 2021
- Full Text
- View/download PDF
35. UPLC‑QTOFMS‑based metabolomic analysis of the serum of hypoxic preconditioning mice.
- Author
-
Liu J, Zhan G, Chen D, Chen J, Yuan ZB, Zhang EL, Gao YX, Xu G, Sun BD, Liao W, and Gao YQ
- Subjects
- Amino Acids blood, Animals, Discriminant Analysis, Disease Models, Animal, Ischemic Preconditioning, Least-Squares Analysis, Lysophosphatidylcholines blood, Male, Metabolomics, Mice, Mice, Inbred BALB C, Palmitoylcarnitine blood, Principal Component Analysis, Chromatography, High Pressure Liquid, Hypoxia, Spectrometry, Mass, Electrospray Ionization
- Abstract
Hypoxic preconditioning (HPC) is well‑known to exert a protective effect against hypoxic injury; however, the underlying molecular mechanism remains unclear. The present study utilized a serum metabolomics approach to detect the alterations associated with HPC. In the present study, an animal model of HPC was established by exposing adult BALB/c mice to acute repetitive hypoxia four times. The serum samples were collected by orbital blood sampling. Metabolite profiling was performed using ultra‑performance liquid chromatography‑quadrupole time‑of‑flight mass spectrometry (UPLC‑QTOFMS), in conjunction with univariate and multivariate statistical analyses. The results of the present study confirmed that the HPC mouse model was established and refined, suggesting significant differences between the control and HPC groups at the molecular levels. HPC caused significant metabolic alterations, as represented by the significant upregulation of valine, methionine, tyrosine, isoleucine, phenylalanine, lysophosphatidylcholine (LysoPC; 16:1), LysoPC (22:6), linoelaidylcarnitine, palmitoylcarnitine, octadecenoylcarnitine, taurine, arachidonic acid, linoleic acid, oleic acid and palmitic acid, and the downregulation of acetylcarnitine, malate, citrate and succinate. Using MetaboAnalyst 3.0, a number of key metabolic pathways were observed to be acutely perturbed, including valine, leucine and isoleucine biosynthesis, in addition to taurine, hypotaurine, phenylalanine, linoleic acid and arachidonic acid metabolism. The results of the present study provided novel insights into the mechanisms involved in the acclimatization of organisms to hypoxia, and demonstrated the protective mechanism of HPC.
- Published
- 2017
- Full Text
- View/download PDF
36. Megakaryocytic leukemia 1 directs a histone H3 lysine 4 methyltransferase complex to regulate hypoxic pulmonary hypertension.
- Author
-
Chen D, Yang Y, Cheng X, Fang F, Xu G, Yuan Z, Xia J, Kong H, Xie W, Wang H, Fang M, Gao Y, and Xu Y
- Subjects
- Animals, Disease Models, Animal, Endothelium, Vascular metabolism, Endothelium, Vascular physiopathology, Epigenesis, Genetic, Hypertension, Pulmonary etiology, Hypertension, Pulmonary metabolism, Hypoxia etiology, Hypoxia metabolism, Leukemia, Megakaryoblastic, Acute genetics, Leukemia, Megakaryoblastic, Acute metabolism, Mice, Mice, Knockout, Polymerase Chain Reaction, Pulmonary Wedge Pressure, Trans-Activators biosynthesis, Transcriptional Activation, Vasodilation, Gene Expression Regulation, Hypertension, Pulmonary genetics, Hypoxia genetics, Leukemia, Megakaryoblastic, Acute complications, RNA genetics, Trans-Activators genetics
- Abstract
Enhanced interaction between vascular endothelial cells and circulating leukocytes, as a result of transcriptional activation of cell adhesion molecules (CAM), helps establish a proinflammatory milieu contributing to the pathogenesis of chronic hypoxia-induced pulmonary hypertension. The molecular switch that dictates CAM transactivation is not clearly defined. Our goal was to determine the involvement of the transcriptional modulator megakaryocytic leukemia 1 (MKL1), also known as myocardin-related transcription factor A (MRTF-A), in CAM transactivation and the underlying mechanism. We report here that compared with wild-type littermates, MKL1/MRTF-A knockout mice were more resistant to the development of hypoxia-induced pulmonary hypertension when exposed to low oxygen pressure. Notably, CAM induction in knockout mice was significantly attenuated with a concomitant reduction of leukocyte adhesion. In cultured vascular endothelial cells, overexpression of MKL1/MRTF-A enhanced, whereas depletion of MKL1/MRTF-A dampened, hypoxia-induced CAM transactivation. In response to hypoxia, MKL1/MRTF-A formed a complex with NF-κB on the CAM promoters. Of interest, MKL1/MRTF-A was responsible for recruiting a histone H3 lysine 4 methyltransferase complex to the CAM promoters. Finally, endothelial-specific silencing of ASH2 and WDR5, 2 key components of the histone H3 lysine 4 methyltransferase complex, ameliorated hypoxia-induced pulmonary hypertension in mice. In conclusion, our data suggest that MKL1/MRTF-A, by coordinating key epigenetic alterations on CAM promoters, provides a critical link to hypoxia-induced endothelial malfunction and contributes to the pathogenesis of hypoxia-induced pulmonary hypertension., (© 2015 American Heart Association, Inc.)
- Published
- 2015
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.