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468 results on '"Cheng, Seng"'

5. Molecular characterisation of nerol dehydrogenase gene (PmNeDH) from Persicaria minor in response to stress-related phytohormones

Author

Cheng Seng Tan, Nur-Athirah Abd-Hamid, Jin Kiat Chew, Maizom Hassan, Ismanizan Ismail, Chyan Leong Ng, and Zamri Zainal

Subjects
nerol dehydrogenase, defence-related gene, phytohormones, persicaria minor, Plant culture, SB1-1110, Plant ecology, QK900-989
Abstract

Citral is a mixture of neral and geranial, which are of great interest to the fragrance industry due to its lemon-scented aroma. A newly characterized nerol dehydrogenase of Persicaria minor (PmNeDH) from our recent findings has shown a capacity to convert citral from nerol. Differential gene expression analysis revealed that the expression level of PmNeDH was highly upregulated during early treatment of several stress-related phytohormones i.e. methyl jasmonate (MeJA), salicylic acid (SA) and abscisic acid (ABA). SA and ABA were shown to have a prolonged effect on PmNeDH expression level until second day of treatment. The findings were in agreement with the cis-regulatory elements predicted from the gene promoter. The phylogenetic relationship of PmNeDH with its homologs from the medium-chain dehydrogenases/reductases (MDR) superfamily was also mentioned. In this study, we proposed a possible biological function of PmNeDH gene in P. minor, which might play significant roles in plant defence mechanism.

Published
2019
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6. Relicensing Practices of Taxi Drivers and Crane Operators Aged 70 Years and above in Singapore

Author

Mei Leng Chan, Yoko Wong, Karen Tan, Joon Cheng Seng, Stephanie Jia Xian Ho, Claire Jing Wen Wong, and Gerald Choon-Huat Koh

Subjects
taxi driver, crane operator, licensing, evidence-based practice, assessment, ageing, Social Sciences
Abstract

Singapore is a rapidly ageing society, underpinned by national policies to promote successful and active ageing. Whilst older worker participation in the workforce is encouraged, policymakers are challenged to ensure that work competency is not compromised with any normal age- and/or health-related changes. This paper will briefly outline how policymakers responded to the needs of two subgroups of older workers aged 70 years and above (taxi drivers; crane operators) who desire to continue working in the last two decades. Whilst a mandatory retirement age policy exists for older taxi drivers in Singapore, there is none for older crane operators. Despite this, stricter relicensing protocols were introduced for both types of workers, with active collaboration involving additional occupational therapy services for functional work competency assessments to complement the routine medical fitness screening. Comparisons will be made of these two relicensing frameworks, including the mention of any relevant studies to align with the call of evidence-based practices. In mid-2020, the relicensing policy for older taxi drivers was revised based on findings of a retrospective national database study. Currently, a 4-year national database study on older crane operators aged 70 years and above is being undertaken with preliminary findings to be reported.

Published
2022
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7. Fetal gene therapy for neurodegenerative disease of infants

Author

Massaro, Giulia, Mattar, Citra N. Z., Wong, Andrew M. S., Sirka, Ernestas, Buckley, Suzanne M. K., Herbert, Bronwen R., Karlsson, Stefan, Perocheau, Dany P., Burke, Derek, Heales, Simon, Richard-Londt, Angela, Brandner, Sebastian, Huebecker, Mylene, Priestman, David A., Platt, Frances M., Mills, Kevin, Biswas, Arijit, Cooper, Jonathan D., Chan, Jerry K. Y., Cheng, Seng H., Waddington, Simon N., and Rahim, Ahad A.

Published
2018
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8. 'Benign' temporal lobe epilepsy with hippocampal sclerosis: A forgotten entity?

Author

Emmanuel Cheng Seng Leong and Udaya Seneviratne

Subjects
Temporal lobe epilepsy, Hippocampal sclerosis, Late-onset, Benign, Neurology. Diseases of the nervous system, RC346-429, Neurophysiology and neuropsychology, QP351-495
Abstract

Mesial temporal lobe epilepsy, a well-characterized epilepsy syndrome, is most commonly accompanied by the pathological feature of hippocampal sclerosis. Patients with mesial temporal lobe epilepsy with hippocampal sclerosis (MTLE-HS) typically present in childhood to early adolescence. We describe a cohort of patients who presented with their first epileptic seizure in late adulthood with atypical features. We characterized five patients with late-onset MTLE-HS by describing their demographics, electroclinical features, imaging, autoantibody status, and response to antiseizure medication (ASM) therapy. All five patients had their first seizure after the age of 50 with no history of initial precipitating incidents. None of our patients had positive serum or CSF autoantibodies and they have all remained seizure-free for a minimum of two years on ASMs alone. Two patients had motor vehicle crashes associated with seizures whilst three patients experienced seizures in sleep. A milder form of MTLE, previously described as benign TLE, does exist in routine clinical practice. These patients respond well to ASM therapy, but potentially harmful consequences such as motor vehicle crashes can occur in the untreated.

Published
2020
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11. Microarray dataset of transgenic rice overexpressing Abp57

Author

Lay Wen Tan, Cheng Seng Tan, Zuraida A. Rahman, Hoe-Han Goh, Ismanizan Ismail, and Zamri Zainal

Subjects
Microarray, Abp57, Overexpressing, Rice, Computer applications to medicine. Medical informatics, R858-859.7, Science (General), Q1-390
Abstract

The dataset presented in this article describes microarray experiment of Auxin-binding protein 57, Abp57-overexpressing transgenic rice. The gene expression profiles were generated using Affymetrix GeneChip® Rice (Cn) Gene 1.0 ST Arrays. Total RNA from seedlings tissue of transgenic rice and wildtype, which serve as control were used as starting materials for microarray experiment. Detailed experimental methods and data analysis were described here. The raw and normalized microarray data were deposited into Gene Expression Omnibus (GEO) under accession number GSE99055.

Published
2017
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16. Agarwood Induction: Current Developments and Future Perspectives

Author

Cheng Seng Tan, Nurulhikma Md Isa, Ismanizan Ismail, and Zamri Zainal

Subjects
Aquilaria, agarwood, inducer, high-throughput omics, data integration, Plant culture, SB1-1110
Abstract

Agarwood is a resinous part of the non-timber Aquilaria tree, which is a highly valuable product for medicine and fragrance purposes. To protect the endangered Aquilaria species, mass plantation of Aquilaria trees has become a sustainable way in Asian countries to obtain the highly valuable agarwood. As only physiologically triggered Aquilaria tree can produce agarwood, effective induction methods are long sought in the agarwood industry. In this paper, we attempt to provide an overview for the past efforts toward the understanding of agarwood formation, the evolvement of induction methods and their further development prospects by integrating it with high-throughput omics approaches.

Published
2019
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19. The safety profile of a cationic lipid-mediated cystic fibrosis gene transfer agent following repeated monthly aerosol administration to sheep

Author

Alton, Eric W.F.W., Baker, Alison, Baker, Eilidh, Boyd, A. Christopher, Cheng, Seng H., Coles, Rebecca L., Collie, D. David S., Davidson, Heather, Davies, Jane C., Gill, Deborah R., Gordon, Catherine, Griesenbach, Uta, Higgins, Tracy, Hyde, Stephen C., Innes, J. Alastair, McCormick, Dominique, McGovern, Michael, McLachlan, Gerry, Porteous, David J., Pringle, Ian, Scheule, Ronald K., Shaw, Darren J., Smith, Sionagh, Sumner-Jones, Stephanie G., Tennant, Peter, and Vrettou, Christina

Published
2013
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27. Secreted Gaussia luciferase as a sensitive reporter gene for in vivo and ex vivo studies of airway gene transfer

Author

Griesenbach, Uta, Vicente, Catarina C., Roberts, Megan J., Meng, Cuixiang, Soussi, Samia, Xenariou, Stefania, Tennant, Peter, Baker, Alison, Baker, Eilidh, Gordon, Catherine, Vrettou, Christina, McCormick, Dominique, Coles, Rebecca, Green, Anne-Marie, Lawton, Anna E., Sumner-Jones, Stephanie G., Cheng, Seng H., Scheule, Ronald K., Hyde, Stephen C., Gill, Deborah R., Collie, David D., McLachlan, Gerry, and Alton, Eric W.F.W.

Published
2011
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34. The use of carboxymethylcellulose gel to increase non-viral gene transfer in mouse airways

Author

Griesenbach, Uta, Meng, Cuixiang, Farley, Raymond, Wasowicz, Marguerite Y., Munkonge, Felix M., Chan, Mario, Stoneham, Charlotte, Sumner-Jones, Stephanie G., Pringle, Ian A., Gill, Deborah R., Hyde, Stephen C., Stevenson, Barbara, Holder, Emma, Ban, Hiroshi, Hasegawa, Mamoru, Cheng, Seng H., Scheule, Ronald K., Sinn, Patrick L., McCray, Paul B., Jr., and Alton, Eric W.F.W.

Published
2010
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39. Targeting nuclear RNA for in vivo correction of myotonic dystrophy

Author

Wheeler, Thurman M., Leger, Andrew J., Pandey, Sanjay K., MacLeod, A. Robert, Nakamori, Masayuki, Cheng, Seng H., Wentworth, Bruce M., Bennett, C. Frank, and Thornton, Charles A.

Subjects
RNA -- Physiological aspects -- Health aspects, Myotonic dystrophy -- Genetic aspects -- Physiological aspects -- Development and progression, Oligonucleotides -- Physiological aspects -- Health aspects -- Genetic aspects, Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Antisense oligonucleotides (ASOs) hold promise for gene-specific knockdown in diseases that involve RNA or protein gain-offunction effects. In the hereditary degenerative disease myotonic dystrophy type 1 (DM1), transcripts from the [...]

Published
2012

41. In vivo imaging of gene transfer to the respiratory tract

Author

Griesenbach, Uta, Meng, Cuixiang, Farley, Raymond, Cheng, Seng H., Scheule, Ronald K., Davies, Mark H., Wolstenholme-Hogg, Paul C., ten Hove, Willem, van der Hoeven, Paul, Sinn, Patrick L., McCray, Paul B., Jr., Inoue, Makoto, Geddes, Duncan M., Hasegawa, Mamoru, Frankel, Gad, Wiles, Siouxsie, and Alton, Eric W.F.W.

Published
2008
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44. CNS-targeted gene therapy improves survival and motor function in a mouse model of spinal muscular atrophy

Author

Passini, Marco A., Bu, Jie, Roskelley, Eric M., Richards, Amy M., Sardi, S. Pablo, O'Riordan, Catherine R., Klinger, Katherine W., Shihabuddin, Lamya S., and Cheng, Seng H.

Subjects
Neurons -- Genetic aspects -- Physiological aspects -- Methods, Gene expression -- Physiological aspects -- Genetic aspects -- Methods, Gene therapy -- Methods -- Physiological aspects, Spinal muscular atrophy -- Genetic aspects -- Care and treatment, Central nervous system -- Genetic aspects -- Methods -- Physiological aspects, Health care industry
Abstract

Spinal muscular atrophy (SMA) is a neuromuscular disease caused by a deficiency of survival motor neuron (SMN) due to mutations in the SMN1 gene. In this study, an adeno-associated virus (AAV) vector expressing human SMN (AAV8-hSMN) was injected at birth into the CNS of mice modeling SMA. Western blot analysis showed that these injections resulted in widespread expression of SMN throughout the spinal cord, and this translated into robust improvement in skeletal muscle physiology, including increased myofiber size and improved neuromuscular junction architecture. Treated mice also displayed substantial improvements on behavioral tests of muscle strength, coordination, and locomotion, indicating that the neuromuscular junction was functional. Treatment with AAV8-hSMN increased the median life span of mice with SMA-like disease to 50 days compared with 15 days for untreated controls. Moreover, injecting mice with SMA-like disease with a human SMN-expressing self-complementary AAV vector--a vector that leads to earlier onset of gene expression compared with standard AAV vectors--led to improved efficacy of gene therapy, including a substantial extension in median survival to 157 days. These data indicate that CNS-directed, AAV-mediated SMN augmentation is highly efficacious in addressing both neuronal and muscular pathologies in a severe mouse model of SMA., Introduction Spinal muscular atrophy (SMA) is an autosomal recessive neuromuscular disorder caused by mutations in the survival motor neuron 1 (SMN1) gene and loss of encoded SMN protein (1). The [...]

Published
2010
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48. AAV‐mediated expression of galactose‐1‐phosphate uridyltransferase corrects defects of galactose metabolism in classic galactosemia patient fibroblasts.

Author

Brophy, Megan L., Stansfield, John C., Ahn, Youngwook, Cheng, Seng H., Murphy, John E., and Bell, Robert D.

Abstract

Classic galactosemia (CG) is a rare disorder of autosomal recessive inheritance. It is caused predominantly by point mutations as well as deletions in the gene encoding the enzyme galactose‐1‐phosphate uridyltransferase (GALT). The majority of the more than 350 mutations identified in the GALT gene cause a significant reduction in GALT enzyme activity resulting in the toxic buildup of galactose metabolites that in turn is associated with cellular stress and injury. Consequently, developing a therapeutic strategy that reverses both the oxidative and ER stress in CG cells may be helpful in combating this disease. Recombinant adeno‐associated virus (AAV)‐mediated gene therapy to restore GALT activity offers the potential to address the unmet medical needs of galactosemia patients. Here, utilizing fibroblasts derived from CG patients we demonstrated that AAV‐mediated augmentation of GALT protein and activity resulted in the prevention of ER and oxidative stress. We also demonstrate that these CG patient fibroblasts exhibit reduced CD109 and TGFβRII protein levels and that these effectors of cellular homeostasis could be restored following AAV‐mediated expression of GALT. Finally, we show initial in vivo proof‐of‐concept restoration of galactose metabolism in a GALT knockout mouse model following treatment with AAV‐GALT. [ABSTRACT FROM AUTHOR]

Published
2022
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