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There is considerable concern that many adolescents do not attain the writing competence needed to be successful in school, their personal lives, or the workplace. Ensuring that students acquire this competence is a basic responsibility of schools. In order to meet this objective, teachers need access to effective practices for teaching writing. In this meta-analysis, we examined if teaching writing improved the writing and reading of students in Grades 6-12, and what specific writing treatments enhanced students' writing. Our review included writing treatments tested using an experimental or quasi-experimental design (with pretests) and published and unpublished studies, and computed effect sizes (ESs) for all writing and reading outcomes assessed. Across 406 independent comparisons, yielding 3,514 ESs involving 52,529 students, teaching writing had a positive and statistically detectable impact on students' writing (ES = 0.47) and reading (ES = 0.22). Moreover, a variety of different writing treatments improved students' performance on writing measures. Across all writing outcomes, statistically detectable effects (presented in parentheses) were obtained for comprehensive writing programs (0.47; which included the process approach to writing), strategy instruction (0.76), digital writing tools (0.31), transcription instruction (0.71), computer-assisted instruction (0.32), teaching critical/creative thinking skills for writing (0.27), emulating good models of writing (0.46), feedback (0.34), goal setting (0.44), prewriting activities (0.49), grammar instruction (0.77), sentence instruction (0.73), inquiry (0.92), observing writers/readers, peer assistance (0.41), summarization instruction (0.49), and text structure instruction (0.39). Implications for practice, research, and theory are discussed.

In this study, we examined the relation of observed classroom practices to language and literacy achievement and the moderation of this relation for students from pre-K to sixth grade. A total of 136 studies (N = 107,882 participants) met the inclusion criteria, of which 108 studies were included for meta-analysis and the other 28 studies were narratively synthesized. The average zero-order (r = 0.12) and partial correlations (r[subscript p] = 0.04) were statistically significant but weak in magnitude. The relation was slightly weaker in upper than in lower grade levels, and stronger for observations capturing macro quality and instructional dimension than those capturing micro measurement and emotional or structural dimension, respectively. The relation did not vary by observation duration, frequency, adopted statistical approach, or type of covariates. Taken together with the narrative synthesis, the results highlight the complex nature of classroom observation and a need for more classroom research, particularly on higher grade levels. [For corresponding grantee submission, see ED624703.]

Purpose: Children's ability to adjust one's language according to discourse context is important for success in academic settings. This study examined whether second graders vary in linguistic and discourse features depending on discourse contexts, that is, when describing pictures in contextualized (describing the picture to an examiner while looking at it together) and decontextualized (pretending to describe the picture to a friend while sitting in front of the examiner) conditions. Method: A total of 330 English-speaking second graders in the United States (M[subscript age] = 7.33 years; 53% boys; 55% Caucasian children, 35% African American children) described three pictures in contextualized and decontextualized conditions. Children's picture descriptions were transcribed verbatim and coded for linguistic (e.g., elaborated noun phrase) and discourse (e.g., proper character introduction, degree of decontextualization) features. Results: Type-token ratio was higher in the contextualized condition than in the decontextualized condition, whereas certain types of elaborated noun phrases (e.g., simple descriptive noun phrase, noun phrase with postmodification), coordinating conjunctions, and nonclauses occurred more frequently in the decontextualized condition, controlling for total productivity and student demographics. The proportion of proper character introduction was higher in the decontextualized condition, whereas higher degrees of decontextualization and complex perspective-taking were found in the contextualized condition. Conclusion: Various linguistic and discourse cues illustrated the extent to which primary grade students employ their discourse knowledge when producing oral language. [For the corresponding grantee submission, see ED626814.]

We investigated the relations among theory of mind (ToM), mental state talk, and discourse comprehension. Specifically, we examined the frequency of mental state talk in children's oral recall of narrative texts and informational texts as well as relations among ToM, mental state talk (inclusion of mental state words in the recall of narrative and informational texts), and narrative and informational text comprehension. Results from children in Grade 4 (N = 132; M[subscript age] = 10.39 years) revealed that a greater number of mental state talk instances appeared in children's recall of narrative texts than in their recall of informational texts, but the mean number also differed across texts within a genre. ToM skill predicted the extent of mental state talk in narrative texts and informational texts, and the relation was stronger for narrative texts than for informational texts, after accounting for vocabulary, grammatical knowledge, working memory, and attentional control. Mental state talk in narrative texts was extremely strongly related to narrative comprehension, whereas mental state talk in informational texts was weakly related to informational text comprehension. Results suggest that ToM skill relates to mental state talk in the recall of texts, and both ToM and mental state talk play greater roles in comprehension of narrative texts than in comprehension of informational texts.

This study examined the extent of perspective taking and language features represented in secondary students' text-based analytical writing. We investigated: (1) whether perspective taking is related to writing quality, accounting for language features in writing; (2) whether students' English learner status is related to perspectives represented in their writing; and (3) whether the relation between perspective taking and writing quality differs by the level of language features (e.g., syntactic diversity, appropriate word usage, and tone). Secondary students' text-based analytical essays (N= 195, Grades 7-12) were coded for perspective taking and language features and analyzed using multiple regression. There was a higher frequency of own-side perspectives than dual perspectives. Dual perspective was related to writing quality after accounting for student demographics and grade levels. However, the relation was no longer statistically significant when language features were accounted for. English learners exhibited significantly less own-side perspectives compared to their English-only counterparts, but there was no difference in dual perspectives, which might be due to overall low frequency of dual perspectives represented in students' text-based analytical writing. The findings suggest the roles of both perspective taking and language features in quality writing. [For the corresponding grantee submission, see ED623455.]

Conventional biochemical methods for studying cellular signaling cascades have relied on destructive cell disruption. In contrast, the live cell imaging of fluorescent-tagged transfected proteins offers a non-invasive approach to understanding signal transduction events. One strategy involves monitoring the phosphorylation-dependent shuttling of a fluorescent-labeled kinase between the nucleus and cytoplasm using nuclear localization, export signals, or both. In this paper, we introduce a simple method to visualize intracellular signal transduction in live cells by exploring the translocation properties of PKC from the cytoplasm to the membrane. We fused bait protein to PKC, allowing the bait (RFP-labeled) and target (GFP-labeled) proteins to co-translocate from the cytoplasm to the membrane. However, in non-interacting protein pairs, only the bait protein was translocated to the plasma membrane. To verify our approach, we examined the Raf–MEK–ERK signaling cascade (ERK pathway). We successfully visualized direct Raf1/MEK2 interaction and the KSR1-containing ternary complex (Raf1/MEK2/KSR1). However, the interaction between MEK and ERK was dependent on the presence of the KSR1 scaffold protein under our experimental conditions. [ABSTRACT FROM AUTHOR]

Background: We examined linguistic features in fourth graders' narrative and opinion writing and their relations to writing quality. We analysed narrative and opinion essays in terms of lexical sophistication and diversity as well as syntactic complexity, syntactic accuracy, and morphological complexity. Methods: Data were from English‐speaking students in Grade 4 (N = 129; 50% female) in the United States, majority of whom were from low socioeconomic status background. Paired t tests were used to analyse differences of linguistic features between the two genres. Hierarchical regression models were run to examine how language features are related to writing quality. Results: Words in the narrative task were more diverse and included more diverse verbal inflectional morphemes than those in the opinion task; syntactic complexity was significantly greater in the opinion genre, manifested by longer T‐units and the more frequent use of adverbial and relative clauses. Approximately 80% of T‐units were grammatical and 20% were ungrammatical. Syntactic accuracy and verbal morphological complexity of words were positively related to writing quality in the narrative task. Additionally, syntactic complexity measured by clauses per T‐unit, verbal and nominal morphological complexity of words were uniquely related to writing quality in the opinion genre. Conclusions: The findings highlight the limited extent to which Grade 4 students use complex syntax in their writing. The study also supports the critical role of linguistic features in writing quality and shows both similarities and differences in language use in two important writing genres, narrative and opinion, in elementary grades. Highlights: What is already known about this topic Linguistic features vary across writing genres.Lexical diversity contributes to narrative writing quality for elementary students.The relation between syntactic complexity and writing quality varies by genre. What this paper adds Lexical diversity and nominal morphological complexity were higher in the narrative task; T‐unit length was longer in the opinion task.Syntactic complexity positively and uniquely contributed to writing quality in the opinion task whereas syntactic accuracy positively and uniquely contributed to writing quality in the narrative task.Morphological complexity was uniquely related to writing quality in the narrative and opinion genre. Implications for theory, policy or practice Students in upper elementary grades may benefit from explicit and systematic instruction on salient linguistic features in different genres.Students in upper elementary grades may benefit from explicit and systematic instruction on syntactic accuracy and structures and morphological structures. [ABSTRACT FROM AUTHOR]

Examining the dimensionality of oral discourse language skills in early childhood is crucial in informing theories of language and literacy development. This study examined the factor structure of linguistic and discourse features in oral text production for second graders. A total of 330 English-speaking second graders (M age = 7.33, 53% boys, 55% White) described three pictures in two discourse conditions. Children's picture descriptions were transcribed verbatim and coded for a total of nine linguistic (e.g. elaborated noun phrase, clausal density) and discourse features (e.g. proper character introduction, perspective taking). Results from confirmatory factor analysis showed that a two-factor structure consisting of linguistic and discourse features factors provided the best fit to the data. Findings suggested that various linguistic and discourse features in oral text production can be best described as having two related but distinct factors. [ABSTRACT FROM AUTHOR]

In this study, the case of Pohang Steelworks, a national infrastructure leading the Korean steel industry, was analyzed. Pohang Works, which is a national infrastructure and a national core technology, requires continuous operation, so a risk assessment is required. The survey involved the participation of 30 safety experts from Pohang Works, and additional interviews were conducted to improve the understanding of the survey results. As a result of the qualitative evaluation, the value of the identified risk was obtained, and the value was variable in probability and damage impact. In addition to being prioritized in order of highest risk, they are also placed according to the size of the risk. As a result, the necessary structural and non-structural treatments were proposed for a total of four hazards. Due to the nature of the qualitative evaluation conducted by internal experts, we were able to confirm that internal experts tend to be very aware of and respond to the risks they often face. It is expected that the objectivity of risk assessment results can be increased by adding quantitative methods in future studies. In addition, it is meaningful in that it practically suggests a treatment that focuses on risk management. [ABSTRACT FROM AUTHOR]

We present a multiplexed microfluidic immunohistochemistry (IHC) technology that enables high-throughput analysis of tissue microarrays (TMAs) using the patterns of biomarker barcodes, which consist of a series of expressed linear patterns of specific biomarkers. A multichannel poly(dimethylsiloxane) microfluidic device was reversibly assembled by the pressure of simple equipment for multiplexed IHC on each core of TMA or cell microarray (CMA) section slides. By injecting primary antibodies from different biomarkers independently into each channel, multiplexed immunostaining can be performed on each core of TMA. We confirmed the equal immunostaining quality regardless of the channel orders and core positions in the slide. Four different biomarkers (ER, PR, HER2, and Ki67) were used for the demonstration of distinctive expression patterns on CMAs which consist of six different breast cancer cell lines, and it was confirmed that these bar-like signals could be a biomarker barcode for the TMA core. A biomarker barcode of breast cancer patient-derived TMA was quickly scanned by a slide scanner and compared to the conventional method for breast cancer diagnosis. This "barcode-IHC" concept, which has been verified by performing multiplexed microfluidic IHC on CMA and TMA samples, provides high reproducibility and the potential of high-throughput screening with molecular diagnostic capability. [ABSTRACT FROM AUTHOR]

Background: Low back pain (LBP) has been linked to the degree of lumbar stability, but evaluating lumbar stability has remained a challenge. Previous research has shown that inertial sensors could be used to quantify motor patterns during the wall plank-and-roll (WPR) test, and that LBP may cause deviations in movement from the general motor patterns observed in healthy individuals.Objective: To generalize the lumbar motor patterns during the WPR test in healthy individuals, and to analyze the effect of aging and LBP on the motor patterns during the WPR test.Design: A descriptive, exploratory research with a convenience sample. This study is registered at the Clinical Research Information Service (Korea) under public trial registration numbers KCT0002481 and KCT0002533.Setting: A biomechanics laboratory of a tertiary university hospital.Participants: 57 healthy individuals (23 men 36.7 ± 15.4 years old and 34 women 42.4 ± 17.7 years old) and 17 patients (5 men 48.4 ± 10.9 years old and 12 women 33.7 ± 9.9 years old) with axial LBP.Methods: Participants performed the WPR test with 2 inertial sensors placed on the thoracic spine and sacrum. Relative angles between the sensors were calculated to quantify and examine lumbar motion in 3 anatomical planes: axial twist, kyphosis-lordosis, and lateral bending.Main Outcome Measures: General motor patterns during the WPR test in healthy participants were examined, stratified based on age, and changes based on age were analyzed. Motor patterns of LBP patients were compared with those from the healthy group.Results: Movement in the kyphosis-lordosis and lateral bending axes showed little variation in healthy participants, whereas in the axial twist axis there were 2 dominant patterns. A χ 2 test revealed that the distributions of 2 motor patterns in the axial twist axis between the younger group and the older group were significantly different (P < .05). Furthermore, the older group had decreased lordosis at the static position (P = .02) and at the maximal rotating position (P = .03). Compared with the healthy group, LBP patients showed increasing lateral bending at the maximal rotating position (P = .007) and increased lateral bending excursion angle (P = .04) during the WPR test.Conclusions: A general lumbar motor pattern was observed during the WPR test in the healthy participants, but age contributed to variations in this general pattern. Comparison of motor patterns between healthy individuals and LBP patients revealed a different type of variation in the LBP patients. The results presented should be scrutinized with further research, characterizing specific variations in different subgroups of LBP patients.Level Of Evidence: III. [ABSTRACT FROM AUTHOR]

Surface electromyogram (sEMG) is often used by to objectively measure muscular activity during rehabilitation exercises. sEMG is accurate, but it is unsuitable for uses outside the clinic, and patients can benefit from an unobtrusive device which can be readily used to ubiquitously measure abdominal muscle activation. In this study, we present a pressure sensor system which can be latched onto a belt to measure abdominal muscle activation. sEMG and pressure sensor output were measured in 15 healthy young males during isometric trunk flexion exercise (public trials registration number, KCT0002351), and the results were highly correlated (median R > 0.939). As initial contact force can change the pressure sensor sensitivity, the experiment was performed at two different levels of belt tightness, but the correlations did not significantly improve after tightening the belt, suggesting that the system can be used to ubiquitously and unobtrusively monitor abdominal muscle activity with minimal discomfort. [ABSTRACT FROM AUTHOR]

An integrated microdevice, consisting of a micropump, a passive mixer, a magnetic separation chamber, and a microcapillary electrophoretic channel, was constructed for biobarcode assay based multiplex biological agent detection in a sample-to-answer-out manner within 30 min with high sensitivity. [ABSTRACT FROM AUTHOR]

Inflammation and the immune response in atherosclerosis are complex processes involving local hemodynamics, the interaction of dysfunctional cells, and various pathological environments. Here, a modular multichannel system that mimics the human artery to demonstrate stenosis and inflammation and to study physical and chemical effects on biomimetic artery models is presented. Smooth muscle cells and endothelial cells were cocultured in the wrinkled surface in vivo-like circular channels to recapitulate the artery. An artery-mimicking multichannel module comprised four channels for the fabrication of coculture models and assigned various conditions for analysis to each model simultaneously. The manipulation became reproducible and stable through modularization, and each module could be replaced according to analytical purposes. A chamber module for culture was replaced with a microfluidic concentration gradient generator (CGG) module to achieve the cellular state of inflamed lesions by providing tumor necrosis factor (TNF)-α, in addition to the stenosis structure by tuning the channel geometry. Different TNF-α doses were administered in each channel by the CGG module to create functional inflammation models under various conditions. Through the tunable channel geometry and the microfluidic interfacing, this system has the potential to be used for further comprehensive research on vascular diseases such as atherosclerosis and thrombosis. [ABSTRACT FROM AUTHOR]

Objective: This study aimed to determine the clinical advantage of spindle-view intracytoplasmic sperm injection (SVICSI; a novel technology) over conventional intracytoplasmic sperm injection (cICSI) in patients with poor ovarian response (POR) and previous implantation failure., Methods: The study included 37 patients who underwent SVICSI followed by fresh embryo transfer (FET) at a single fertility clinic from January to December 2022, 58 patients who underwent cICSI followed by FET at the same fertility clinic from January to December 2021 as a control group. All study participants met the Bologna criteria for POR and had at least three or more previous failed embryo transfers., Results: The number of blastocyst transfers was significantly higher in the SVICSI group than in the cICSI group. A good-quality cleavage embryo rate, blastocyst rate, and good-quality blastocyst rate were also significantly higher in the SVICSI group than in the cICSI group. There were no significant differences in the rates of fertilization, implantation, clinical pregnancy, or clinical abortion between the two groups., Conclusion: In patients with POR, those who underwent SVICSI appeared to have better embryos than those who underwent cICSI. However, whether SVICSI improved clinical outcomes such as implantation and pregnancy rates cannot be proven.

Objective: The aim of the present study was to evaluate the associations between hematologic parameters related to systemic inflammation and insulin resistance-associated metabolic parameters in women with polycystic ovary syndrome (PCOS)., Methods: Eighty-two women between the ages of 18 and 35 years who were diagnosed with PCOS were included in this study. A 2-hour 75-g oral glucose tolerance test (OGTT) was administered to all study participants; fasting and postprandial glucose and insulin levels were measured simultaneously during the 2-hour OGTT. Hematologic parameters were derived from a standard complete blood count and a differential count of fasting-state blood samples. The correlations between hematologic parameters and insulin resistance-associated clinical and metabolic parameters were evaluated using the Spearman rank correlation and partial correlation coefficients. Hematologic parameters related to systemic inflammation were compared between the two groups, categorized by the presence or absence of insulin resistance., Results: Significant differences in the absolute neutrophil count, absolute monocyte count, platelet count, and neutrophil-lymphocyte ratio were found between the insulin-resistant group and insulin-nonresistant group. Correlation analysis found that all hematological parameters, except for the platelet-lymphocyte ratio, were associated with at least one insulin resistance-associated metabolic parameter. However, these significant correlations between hematological and metabolic parameters were attenuated after controlling for the effects of other covariates using partial correlation analysis., Conclusion: The association between hematologic parameters indicative of systemic inflammation and insulin resistance-associated metabolic parameters seems to be strongly influenced by other anthropometric covariates in women with PCOS.

Fabrication of a 3D in vitro model that mimics the artery takes an important role in understanding pathological cell behaviors and mechanisms of vascular diseases by proposing an advanced model that can recapitulate a native vessel condition in a controlled manner. Because a model geometry and the structure of cells are significant for the recapitulation of the hemodynamics of arterial and cell functions, it is necessary to mimic geometries and to induce the proper morphology and orientation of the cells when fabricating a model. In this study, smooth muscle cells (SMCs) and endothelial cells (ECs), which were the main elements in the arterial wall, were cocultured in a multichannel device connected with polydimethylsiloxane (PDMS) fluidic chamber modules to parallelly fabricate a pefusable 3D in vitro human artery-mimicking multichannel system. In the coculture model, a circular PDMS channel with a wrinkled-surface guided directionality and contractile morphology to SMCs, and media perfusion induced directionality to a confluent EC layer as in vivo. Protein markers of cells and synthesized extracellular matrices were demonstrated. Because multichannels were connected to a microfluidic module in a device, it was possible to easily control the microenvironmental conditions and to fabricate coculture models in parallel with a single flow system. Coculture models that can be tuned in designs such as diameter, wall shear stress, and geometry of artery disease were constructed by 3D-printed molds to recapitulate various cellular microenvironments and to model vessels effectively. Finally, the effect of wall shear stress on cells was compared using a device with four different degrees of stenosis channels and investigated in parallel.

Background: Low back pain (LBP) has been linked to the degree of lumbar stability, but evaluating lumbar stability has remained a challenge. Previous research has shown that inertial sensors could be used to quantify motor patterns during the wall plank-and-roll (WPR) test, and that LBP may cause deviations in movement from the general motor patterns observed in healthy individuals., Objective: To generalize the lumbar motor patterns during the WPR test in healthy individuals, and to analyze the effect of aging and LBP on the motor patterns during the WPR test., Design: A descriptive, exploratory research with a convenience sample. This study is registered at the Clinical Research Information Service (Korea) under public trial registration numbers KCT0002481 and KCT0002533., Setting: A biomechanics laboratory of a tertiary university hospital., Participants: 57 healthy individuals (23 men 36.7 ± 15.4 years old and 34 women 42.4 ± 17.7 years old) and 17 patients (5 men 48.4 ± 10.9 years old and 12 women 33.7 ± 9.9 years old) with axial LBP., Methods: Participants performed the WPR test with 2 inertial sensors placed on the thoracic spine and sacrum. Relative angles between the sensors were calculated to quantify and examine lumbar motion in 3 anatomical planes: axial twist, kyphosis-lordosis, and lateral bending., Main Outcome Measures: General motor patterns during the WPR test in healthy participants were examined, stratified based on age, and changes based on age were analyzed. Motor patterns of LBP patients were compared with those from the healthy group., Results: Movement in the kyphosis-lordosis and lateral bending axes showed little variation in healthy participants, whereas in the axial twist axis there were 2 dominant patterns. A χ 2 test revealed that the distributions of 2 motor patterns in the axial twist axis between the younger group and the older group were significantly different (P < .05). Furthermore, the older group had decreased lordosis at the static position (P = .02) and at the maximal rotating position (P = .03). Compared with the healthy group, LBP patients showed increasing lateral bending at the maximal rotating position (P = .007) and increased lateral bending excursion angle (P = .04) during the WPR test., Conclusions: A general lumbar motor pattern was observed during the WPR test in the healthy participants, but age contributed to variations in this general pattern. Comparison of motor patterns between healthy individuals and LBP patients revealed a different type of variation in the LBP patients. The results presented should be scrutinized with further research, characterizing specific variations in different subgroups of LBP patients., Level of Evidence: III., (© 2018 American Academy of Physical Medicine and Rehabilitation.)