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4 results on '"Daniela Castelletti"'

1. Correction: The Endocytic Adaptor Eps15 Controls Marginal Zone B Cell Numbers.

Author

Benedetta Pozzi, Stefania Amodio, Caterina Lucano, Anna Sciullo, Simona Ronzoni, Daniela Castelletti, Thure Adler, Irina Treise, Ingrid Holmberg Betsholtz, Birgit Rathkolb, Dirk H. Busch, Eckhard Wolf, Helmut Fuchs, Valérie Gailus-Durner, Martin Hrabě de Angelis, Christer Betsholtz, Stefano Casola, Pier Paolo Di Fiore, and Nina Offenhäuser

Subjects
Medicine, Science
Published
2013
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2. The endocytic adaptor Eps15 controls marginal zone B cell numbers.

Author

Benedetta Pozzi, Stefania Amodio, Caterina Lucano, Anna Sciullo, Simona Ronzoni, Daniela Castelletti, Thure Adler, Irina Treise, Ingrid Holmberg Betsholtz, Birgit Rathkolb, Dirk H Busch, Eckhard Wolf, Helmut Fuchs, Valérie Gailus-Durner, Martin Hrabě de Angelis, Christer Betsholtz, Stefano Casola, Pier Paolo Di Fiore, and Nina Offenhäuser

Subjects
Medicine, Science
Abstract

Eps15 is an endocytic adaptor protein involved in clathrin and non-clathrin mediated endocytosis. In Caenorhabditis elegans and Drosophila melanogaster lack of Eps15 leads to defects in synaptic vesicle recycling and synapse formation. We generated Eps15-KO mice to investigate its function in mammals. Eps15-KO mice are born at the expected Mendelian ratio and are fertile. Using a large-scale phenotype screen covering more than 300 parameters correlated to human disease, we found that Eps15-KO mice did not show any sign of disease or neural deficits. Instead, altered blood parameters pointed to an immunological defect. By competitive bone marrow transplantation we demonstrated that Eps15-KO hematopoietic precursor cells were more efficient than the WT counterparts in repopulating B220⁺ bone marrow cells, CD19⁻ thymocytes and splenic marginal zone (MZ) B cells. Eps15-KO mice showed a 2-fold increase in MZ B cell numbers when compared with controls. Using reverse bone marrow transplantation, we found that Eps15 regulates MZ B cell numbers in a cell autonomous manner. FACS analysis showed that although MZ B cells were increased in Eps15-KO mice, transitional and pre-MZ B cell numbers were unaffected. The increase in MZ B cell numbers in Eps15 KO mice was not dependent on altered BCR signaling or Notch activity. In conclusion, in mammals, the endocytic adaptor protein Eps15 is a regulator of B-cell lymphopoiesis.

Published
2012
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3. Serum thymidine kinase activity in patients with HR-positive/HER2-negative advanced breast cancer treated with ribociclib plus letrozole: Results from the prospective BioItaLEE trial

Author

Luca Malorni, Giampaolo Bianchini, Roberta Caputo, Alberto Zambelli, Fabio Puglisi, Giulia V. Bianchi, Lucia Del Mastro, Ida Paris, Filippo Montemurro, Giacomo Allegrini, Marco Colleoni, Stefano Tamberi, Claudio Zamagni, Marina E. Cazzaniga, Michele Orditura, Valentina Guarneri, Daniela Castelletti, Matteo Benelli, Mariacristina Di Marino, Grazia Arpino, and Michelino De Laurentiis

Subjects
Cancer Research, Thymidine kinase, Oncology, Ribociclib, Letrozole, Advanced breast cancer, Biomarker
Published
2023

4. The endocytic adaptor Eps15 controls marginal zone B cell numbers

Author

Ingrid Holmberg Betsholtz, Benedetta Pozzi, Nina Offenhäuser, Anna Sciullo, Simona Ronzoni, Irina Treise, Daniela Castelletti, Helmut Fuchs, Thure Adler, Eckhard Wolf, Stefano Casola, Caterina Lucano, Martin Hrabě de Angelis, Pier Paolo Di Fiore, Dirk H. Busch, Stefania Amodio, Birgit Rathkolb, Valerie Gailus-Durner, and Christer Betsholtz

Subjects
Male, B Cells, Mouse, Endocytic cycle, Gene Expression, lcsh:Medicine, Cell Count, Gene Knockout Techniques, Mice, 0302 clinical medicine, Marginal zone B-cell, Molecular Cell Biology, Lymphoid Organs, lcsh:Science, Bone Marrow Transplantation, 0303 health sciences, B-Lymphocytes, Multidisciplinary, Receptors, Notch, Signal transducing adaptor protein, Animal Models, Endocytosis, Cell biology, medicine.anatomical_structure, Medicine, Female, Research Article, Immune Cells, Immunology, Bone Marrow Cells, Thymus Gland, Biology, Clathrin, 03 medical and health sciences, Model Organisms, medicine, Genetics, Synaptic vesicle recycling, Animals, Lymphopoiesis, B cell, 030304 developmental biology, Adaptor Proteins, Signal Transducing, lcsh:R, Immune System, biology.protein, Clinical Immunology, lcsh:Q, Bone marrow, Gene Function, 030215 immunology
Abstract

Eps15 is an endocytic adaptor protein involved in clathrin and non-clathrin mediated endocytosis. In Caenorhabditis elegans and Drosophila melanogaster lack of Eps15 leads to defects in synaptic vesicle recycling and synapse formation. We generated Eps15-KO mice to investigate its function in mammals. Eps15-KO mice are born at the expected Mendelian ratio and are fertile. Using a large-scale phenotype screen covering more than 300 parameters correlated to human disease, we found that Eps15-KO mice did not show any sign of disease or neural deficits. Instead, altered blood parameters pointed to an immunological defect. By competitive bone marrow transplantation we demonstrated that Eps15-KO hematopoietic precursor cells were more efficient than the WT counterparts in repopulating B220(+) bone marrow cells, CD19(-) thymocytes and splenic marginal zone (MZ) B cells. Eps15-KO mice showed a 2-fold increase in MZ B cell numbers when compared with controls. Using reverse bone marrow transplantation, we found that Eps15 regulates MZ B cell numbers in a cell autonomous manner. FACS analysis showed that although MZ B cells were increased in Eps15-KO mice, transitional and pre-MZ B cell numbers were unaffected. The increase in MZ B cell numbers in Eps15 KO mice was not dependent on altered BCR signaling or Notch activity. In conclusion, in mammals, the endocytic adaptor protein Eps15 is a regulator of B-cell lymphopoiesis.

Published
2012

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